锥形玻璃纳米孔中溶液浓度和pH值对整流的影响

为了探索锥形纳米孔整流的机理，以硼硅酸盐玻璃毛细管为材料，采用微电极拉制仪P 2000拉制了内径20～50 nm的玻璃锥形纳米孔。孔内注满氯化钠盐溶液后，采用膜片钳对该类纳米孔的整流效应进行了实验研究。实验发现，锥形玻璃纳米孔的整流比随浓度先增大后减小，在溶液浓度为10～50 mmol/L范围内出现最大整流比。当溶液pH值高于玻璃等电点时，随着溶液pH值的增大，整流比随之增大；当溶液pH值低于玻璃等电点时，玻璃表面发生壁面电荷倒置，整流方向发生改变。实验结果表明，玻璃锥形纳米孔整流效应受到溶液浓度和溶液pH值共同影响，纳米孔内壁不经过任何化学修饰就可以发生电荷倒置。

新型有序纳米介孔生物活性玻璃对成骨细胞IGF-Ⅱ基因调控的影响

目的研究新型有序纳米介孔生物活性玻璃的体外生物活性及其对鼠成骨细胞的IGF-Ⅱ基因表达效果,探讨其可能的调控机制。方法定量选取新型的生物活性玻璃MBG(80S15C),浸泡于MEM培养液中,取离子浸出液培养大鼠成骨细胞,使用MTT法和PNPP法测定成骨细胞增殖和ALP的活性,RT-PCR法测定IGF-Ⅱ的mRNA表达,ELISA法测定IGF-Ⅱ蛋白和IGFBP的浓度。结果实验组成骨细胞的ALP活性为对照组的126%,IGF-Ⅱ基因表达为对照组的126%,IGF-Ⅱ蛋白和IGFBP的浓度分别是对照组175%和237%,细胞增殖为对照组的92%。结论MBG的离子浸出液能够明显促进成骨细胞的分化,其机制可能是通过诱导激活IGF-Ⅱ基因的表达,增加IGF-Ⅱ蛋白,IGFBP的合成和分泌,进而促进成骨细胞的分化。

纳米微孔氧化硅玻璃在与Nd^(3+)-ZBLAN复合过程中的失透(英文)

在将玻璃片置于Nd3+-ZBLAN熔体中对微孔灌注复合时,纳米微孔氧化硅玻璃片中部发生失透,失透部分出现50-100μm晶态鳞片,而鳞片中仍然存在纳米微孔。复合层仍处于非晶态。氧化硅被孔中氢氧化钠腐蚀形成晶籽,而孔中的高气压有助于结晶的发展。

Glass capillary nanopore for single molecule detection

Single molecule detection based on nanopore technology is a very promising approach for medical diagnostics, drug therapy and even DNA sequencing. Compared with other biological nanopores and solid-state nanopores, the glass capillary nanopore has low cost, easy availability and stable mechanical characteristics, thus it has been widely used in the nanopore technology for single molecule detection. In this review, we will focus on the studies of the glass nanopore sensors. The popular glass nanopore fabrication methods would be introduced, and the applications of glass nanopores in the detection of nanoparticles, proteins and DNA molecules would be presented. We hope this review will help widen field of vision and promote the development of the nanopore technology based on the glass capillary nanopores.