葫芦瓶,顾名思义就是造型如葫芦的瓶子,其形状特点有三个：小口,束腰,上小下大。将葫芦瓶列入吉祥宝座的理由可以有一大堆：葫芦与＂福禄＂谐音（2015HAPPY NEW YEAR,图1）;＂瓶＂字暗合＂平安＂;葫芦瓶分上、下两段,不就是一个＂吉＂...葫芦瓶,顾名思义就是造型如葫芦的瓶子,其形状特点有三个：小口,束腰,上小下大。将葫芦瓶列入吉祥宝座的理由可以有一大堆：葫芦与＂福禄＂谐音（2015HAPPY NEW YEAR,图1）;＂瓶＂字暗合＂平安＂;葫芦瓶分上、下两段,不就是一个＂吉＂字吗？葫芦又沾了仙气,在道教中葫芦有法器的作用,道教神仙——八仙中的铁拐李,随身携带的法宝就是葫芦（2004—15M）。因此葫芦瓶在我国传统工艺品中比较常见,陶瓷质地的更多。它还常被当作陈设品,以符合＂趋吉避凶＂、＂迎祥纳福＂的民俗。展开更多
Mexico is a large producer of table grape （Vitis vinifera L.） and therefore it is important to develop protocols to store the grape varieties germplasm. The objective of the present work was to design a protocol for...Mexico is a large producer of table grape （Vitis vinifera L.） and therefore it is important to develop protocols to store the grape varieties germplasm. The objective of the present work was to design a protocol for the cryopreservation by vitrification of zygotic embryos of V. vinifera cv. ＂Red Globe＂ and evaluate possible epigenetics changes. The plant vitrification solution 2 （PVS2） was utilized before the utilization of liquid nitrogen （LN）. The effect of this protocol on embryo viability was tested by the triphenyl-tetrazolium chloride solution, as well as by the in vitro development of grape embryos into plantlet. A cDNA expression library of grape zygotic embryos was created to isolate expressed sequence tags of several DNA methyltrasferases. Gene expression of domains rearranged methyltransferase type 1 （DMR1） and DNA （cytosine-5）-methyltransferase 1 （MET1-2） isozymes was analyzed by quantitative reverse transcriptase PCR. The optimal conditions for vitrification were 10 min in 50% PVS2, followed by I0 min in 100% PVS2. Under these conditions, about 30% of plantlet was obtained from embryos after cryopreservation. It was recorded a reduction in the MET1-2 gene expression, which plays a role in the maintenance of DNA methylation. It is possible to cryopreserve viable grape zygotic embryos, although the treatment seems to induce alterations in the normal DNA methylation pattern of the zygotic embryo genome.展开更多
文摘葫芦瓶,顾名思义就是造型如葫芦的瓶子,其形状特点有三个：小口,束腰,上小下大。将葫芦瓶列入吉祥宝座的理由可以有一大堆：葫芦与＂福禄＂谐音（2015HAPPY NEW YEAR,图1）;＂瓶＂字暗合＂平安＂;葫芦瓶分上、下两段,不就是一个＂吉＂字吗？葫芦又沾了仙气,在道教中葫芦有法器的作用,道教神仙——八仙中的铁拐李,随身携带的法宝就是葫芦（2004—15M）。因此葫芦瓶在我国传统工艺品中比较常见,陶瓷质地的更多。它还常被当作陈设品,以符合＂趋吉避凶＂、＂迎祥纳福＂的民俗。
文摘Mexico is a large producer of table grape （Vitis vinifera L.） and therefore it is important to develop protocols to store the grape varieties germplasm. The objective of the present work was to design a protocol for the cryopreservation by vitrification of zygotic embryos of V. vinifera cv. ＂Red Globe＂ and evaluate possible epigenetics changes. The plant vitrification solution 2 （PVS2） was utilized before the utilization of liquid nitrogen （LN）. The effect of this protocol on embryo viability was tested by the triphenyl-tetrazolium chloride solution, as well as by the in vitro development of grape embryos into plantlet. A cDNA expression library of grape zygotic embryos was created to isolate expressed sequence tags of several DNA methyltrasferases. Gene expression of domains rearranged methyltransferase type 1 （DMR1） and DNA （cytosine-5）-methyltransferase 1 （MET1-2） isozymes was analyzed by quantitative reverse transcriptase PCR. The optimal conditions for vitrification were 10 min in 50% PVS2, followed by I0 min in 100% PVS2. Under these conditions, about 30% of plantlet was obtained from embryos after cryopreservation. It was recorded a reduction in the MET1-2 gene expression, which plays a role in the maintenance of DNA methylation. It is possible to cryopreserve viable grape zygotic embryos, although the treatment seems to induce alterations in the normal DNA methylation pattern of the zygotic embryo genome.
