Objective: Elf-1 is a member of the proto-oncogenes Ets-related transcription factor family and over-expressed in many human tumors, Ki-67 is an important nuclear antigen associated with cell proliferation. This study...Objective: Elf-1 is a member of the proto-oncogenes Ets-related transcription factor family and over-expressed in many human tumors, Ki-67 is an important nuclear antigen associated with cell proliferation. This study investigated the expression of Elf-1 and Ki-67 in non-small-cell lung cancer(NSCLC) and studied their correlation with the clinicopathological features. Methods: Tissue microarray from 64 cases lung cancer tissue and 10 cases normal lung tissue was constructed, immunohistochemical method was used to evaluate the protein expressions of Elf-1 and Ki-67, correlations of the expression of Elf-1 and Ki-67 to clinicopathological features of NSCLC were analyzed. Results: Expression of Elf-1 and Ki-67 in NSCLC tissues were significantly higher than in normal lung tissues(P < 0.05), the positive rate of Elf-1 and Ki-67 was 73.44% and 64.06% in NSCLC group, Overexpression of Elf-1 in NSCLC was significantly related to histopathological grading, different clinical staging and the intensity of ELF-1 expression was significantly higher in the group with lymph node metastasis than that without(P < 0.05). Overexpression of Ki-67 was also closely related to tumor differentiation, clinical stages and lymph node metastasis(P < 0.05). In addition positive correlation was found between the expressive intensity of Elf-1 and Ki-67(τ = 0.295, P = 0.018). Conclusion: The high expression and positive correlation of Elf-1 and Ki-67 in NSCLC suggest that they probably play a role in onset and progression of lung cancer, united detecting their expression could be used as an valuable molecular biological index for predicting the malignant behavior and early diagnosis of NSCLC.展开更多
Biological and synthetic surfactants were compared in terms of their ability to reduce interfacial tension, change the thermodynamic characteristics of a pre-conditioned surface, and to modify the rheological properti...Biological and synthetic surfactants were compared in terms of their ability to reduce interfacial tension, change the thermodynamic characteristics of a pre-conditioned surface, and to modify the rheological properties of their respective formulations at two different temperatures. Both classes of suffactants were able to reduce the inteffacial tension of their formulations to a similar level. However, the biosurfactants were more effective than the synthetics surfactants. Biosurfactants also altered the surface properties of stainless steel, rendering it hydrophilic. Microbial adhesion to stainless steel conditioned with biosurfactants was found to be thermodynamically unfavorable for all microbial strains tested. A linear relationship between shear stress and shear rate was obtained across a range of experimental conditions for all surfactant mixtures, indicating that all formulations behaved as Newtonian fluids.展开更多
基金Supported by grants from the Science and Technology Development Project of Yantai(No.2008162)Natural Sciences Foundation of Shandong(No.ZR2013HL004)Science and Technology Important Project of Binzhou Medical College(No.BY20bkjzd04)
文摘Objective: Elf-1 is a member of the proto-oncogenes Ets-related transcription factor family and over-expressed in many human tumors, Ki-67 is an important nuclear antigen associated with cell proliferation. This study investigated the expression of Elf-1 and Ki-67 in non-small-cell lung cancer(NSCLC) and studied their correlation with the clinicopathological features. Methods: Tissue microarray from 64 cases lung cancer tissue and 10 cases normal lung tissue was constructed, immunohistochemical method was used to evaluate the protein expressions of Elf-1 and Ki-67, correlations of the expression of Elf-1 and Ki-67 to clinicopathological features of NSCLC were analyzed. Results: Expression of Elf-1 and Ki-67 in NSCLC tissues were significantly higher than in normal lung tissues(P < 0.05), the positive rate of Elf-1 and Ki-67 was 73.44% and 64.06% in NSCLC group, Overexpression of Elf-1 in NSCLC was significantly related to histopathological grading, different clinical staging and the intensity of ELF-1 expression was significantly higher in the group with lymph node metastasis than that without(P < 0.05). Overexpression of Ki-67 was also closely related to tumor differentiation, clinical stages and lymph node metastasis(P < 0.05). In addition positive correlation was found between the expressive intensity of Elf-1 and Ki-67(τ = 0.295, P = 0.018). Conclusion: The high expression and positive correlation of Elf-1 and Ki-67 in NSCLC suggest that they probably play a role in onset and progression of lung cancer, united detecting their expression could be used as an valuable molecular biological index for predicting the malignant behavior and early diagnosis of NSCLC.
文摘Biological and synthetic surfactants were compared in terms of their ability to reduce interfacial tension, change the thermodynamic characteristics of a pre-conditioned surface, and to modify the rheological properties of their respective formulations at two different temperatures. Both classes of suffactants were able to reduce the inteffacial tension of their formulations to a similar level. However, the biosurfactants were more effective than the synthetics surfactants. Biosurfactants also altered the surface properties of stainless steel, rendering it hydrophilic. Microbial adhesion to stainless steel conditioned with biosurfactants was found to be thermodynamically unfavorable for all microbial strains tested. A linear relationship between shear stress and shear rate was obtained across a range of experimental conditions for all surfactant mixtures, indicating that all formulations behaved as Newtonian fluids.
