To investigate the roles of maspin and kai1 expression in tumorigenesis and progression of gastric cancer. Methods Maspin and kai1 expressions were detected in normal gastric mucosa (n = 182), gastric dysplasia (n = 6...To investigate the roles of maspin and kai1 expression in tumorigenesis and progression of gastric cancer. Methods Maspin and kai1 expressions were detected in normal gastric mucosa (n = 182), gastric dysplasia (n = 69), and gastric cancer (n = 113) by immunohisto-chemistry. Their expressions were compared with clinicopathological parameters of tumors. Relationship between maspin and kai1 expression was also concerned in gastric cancer. Results The positive rates of maspin expression were 79.8% (145/182), 75.4% (52/69), and 50.4% (57/113) in normal gastric mucosa, gastric dysplasia, and gastric cancer, while those of kai1 expression were 81.9% (149/182), 65.2% (49/69), and 58.4% (66/113) in corresponding tissues respectively. Gastric cancer less frequently expressed maspin than the normal gastric mucosa and gastric dysplasia (P < 0.05), while dysplasia and cancer showed less frequent expression of kai1 than normal mucosa (P < 0.05). Maspin expression showed negative association with invasive depth, metastasis, Lauren’s and histological classifications (P < 0.05), but not with tumor size, Borrmann’s classification, growth pattern or TNM staging (P > 0.05). Kai1 expression was negatively correlated with invasive depth, metastasis, growth pattern, Lauren’s and histo-logical classifications (P < 0.05), but not with tumor size, Borrmann’s classification or TNM staging (P > 0.05). Maspin and kai1 were collaboratively expressed in gastric cancer (P < 0.05). Conclusions Down-regulated expressions of maspin and kai1 play an important role in gastric carcinogenesis. Abnormal expression of maspin and kai1 might have inhibitory effects on invasion and metastasis of gastric cancer and act as an effe-ctive and objective marker to indicate the pathobiological behaviors of gastric cancer.展开更多
Long-term field experiment was established in 1978 on a coastal paddy soil to determine the effect of applicationof pig manure, rice straw and chemical N fertilizer on the physical property and humus characteristics o...Long-term field experiment was established in 1978 on a coastal paddy soil to determine the effect of applicationof pig manure, rice straw and chemical N fertilizer on the physical property and humus characteristics of soil . Resultsshowed that the porosity, the microstructural coefficient, the reactivities of organic C and N, the ΔlogK value, thedegree of oxidation stability, the contents of O-alkyl C and alkyl C, and the ratio of aliphatic C to aromatic C ofhumic acid from soils received organic manure increased, whereas, the ratio of < 10 μm to >10 μm ofmicroaggregates, the humification degree of humus, the degree of organo-mineral complexation, the number-averagemolecular weight, the C/ H ratio. the contents of carboxyl and aromatic C of HAs in them decreased. These resultsindicated that the application of organic manure not only improved the physical property of the paddy soil but alsomade the HA more aliphatic in structure and younger in origin.展开更多
Nowadays, Computer Assisted Instruction is widespread in universities while the teaching effect is far from satisfying. Therefore. it is necessary to explore the current situation of Application of Computer Assisted I...Nowadays, Computer Assisted Instruction is widespread in universities while the teaching effect is far from satisfying. Therefore. it is necessary to explore the current situation of Application of Computer Assisted Instruction, analyze irreplaceable advantages as well as causes of ineffectiveness in teaching, and and bring forward the approach to solving them.展开更多
Purpose: This paper is a report about the concept analysis of continuity of care, to get further understanding of continuity of care. Methods: The Walker and Avant concept analysis approach was applied in this paper...Purpose: This paper is a report about the concept analysis of continuity of care, to get further understanding of continuity of care. Methods: The Walker and Avant concept analysis approach was applied in this paper. Results: The three attributes include: seamless transfer, flexible reaction and effective interaction. The antecedents were changed needs and patient centred care. In addition, the continuity of care had a significant effect on patients’ outcome, satisfaction and costs. Conclusions: This analysis provides nurses an understanding about continuity of care in both patient’s and care provider’s perspective. Continuity of care is a tailored services provided by care givers, which is an essential element of healthcare system.展开更多
The presented work is based on laboratory testing of seed germination speed, emergence and seedling growth under different stress conditions simulated by subnormal water revel, extreme high and low temperatures. It al...The presented work is based on laboratory testing of seed germination speed, emergence and seedling growth under different stress conditions simulated by subnormal water revel, extreme high and low temperatures. It allows one to eliminate the plant materials (initial breeding materials and cultivars) which do not tolerate extreme temperatures and temperature changes during germination, have low water use efficiency and is intolerant to abiotic stressors all right at the seed level. It was confirmed that these genotypes also have poor t^eld emergence and initial root growth implications for further vegetation periods, mainly for over wintering and spring regeneration which has significant influence on the yield. The method also represents the tool for screening genetic resources with the resistance to the abiotic stressors and this technology process is also acceptable for other crops. The results confirm the importance of the seed and root characteristics for crop production. The deteriorating quality of soil in recent years, increasing variability of weather and long periods of drought directly support the need to intensify activities in this research. Obtained results also show the influence of seed quality characteristics on roots and above ground parts of the plant. A relationship exists also vice versa.展开更多
A case of hailstorm process occurring on 24 June 2006 in northwestern China was studied using satellite retrieval methodology. The particle effective radius (re) in the cloud tops was calculated by the reflectance in ...A case of hailstorm process occurring on 24 June 2006 in northwestern China was studied using satellite retrieval methodology. The particle effective radius (re) in the cloud tops was calculated by the reflectance in the 3.7 μm channel, and cloud-top microphysical properties were vividly represented using the RGB visual multispectral classification scheme. The microphysical zones of clouds and the processes of hail formation and develop-ment are inferred using the relations of cloud-top temperature (T) versus re for the tops of convective clouds. The results show that particle effective radius was smaller near the cloud base of hailstorm. There was a deep zone of diffusional droplet growth at the low level where the particles grew slowly with height, and there existed an evident area of small ice particles in the cloud top, suggesting the existence of a strong updraft in the clouds. The low glaciated temperature indicated a great depth from the cloud base to the glaciation height, which provided a deep layer of supercooled water for hail growth.展开更多
Restricted genetic diversity can supply only a limited number of elite genes for modern plant cultivation and transgenesis.In this study,we demonstrate that rational design enables the engineering of geranyl-geranyl d...Restricted genetic diversity can supply only a limited number of elite genes for modern plant cultivation and transgenesis.In this study,we demonstrate that rational design enables the engineering of geranyl-geranyl diphosphate synthase(NtGGPPS),an enzyme of the methylerythritol phosphate pathway(MEP)in the model plant Nicotiana tabacum.As the crucial bottleneck in carotenoid biosynthesis,NtGGPPS1 interacts with phytoene synthase(NtPSY1)to channel GGPP into the production of carotenoids.Loss of this enzyme in the ntggpps1 mutant leads to decreased carotenoid accumulation.With the aim of enhanc-ing NtGGPPS1 activity,we undertook structure-guided rational redesign of its substrate binding pocket in combination with sequence alignment.The activity of the designed NtGGPPS1(a pentuple mutant of five sites V154A/I161L/F218Y/I209S/V233E,d-NtGGPPS1)was measured by a high-throughput colorimetric assay.d-NtGGPPS1 exhibited significantly higher conversion of IPP and each co-substrate(DMAPP~1995.5-fold,GPP~25.9-fold,and FPP~16.7-fold)for GGPP synthesis compared with wild-type NtGGPPS1.Importantly,the transient and stable expression of d-NtGGPPS1 in the ntggpps1 mutant increased carotenoid levels in leaves,improved photosynthetic efficiency,and increased biomass relative to NtGGPPS1.These findings provide a firm basis for the engineering of GGPPS and will facilitate the development of quality and yield traits.Our results open the door for the structure-guided rational design of elite genes in higher plants。展开更多
This paper tries to find interaction rules between members and self-reform rules of members in some scientific research laboratories at a graduate school. The candidate of rules are extracted from the personality desc...This paper tries to find interaction rules between members and self-reform rules of members in some scientific research laboratories at a graduate school. The candidate of rules are extracted from the personality description sentences about factors: extroversion, agreeableness, conscientiousness, neuroticism and openness in the big five theory of personality psychology. In this paper, interaction and self-reform rules are not described by personalities themselves; instead, they are described by corresponding phenomena: activity, empathy, persistence, autonomy, and analytical ability which appear in the behavior side. Each member's initial value is determined from the main 5-factor personality investigation to the member, and the target value is given by the member's self-declaration. The rule selection is carried out by the genetic algorithm, where a sigmoid function is introduced in the renewal algorithm of the value, whereby a difficulty of setting the renewal parameters is avoided. An analysis is added about the obtained rules, and the validity of this approach and the subjects for future study are discussed. Finally, consideration is extended to the effect of environment on the behavior of members to reinforce the set of rules.展开更多
We explore 10-nm wide Si nanowire (SiNW) field-effect transistors (FETs) for logic applications, via the fabrication and testing of SiNW-based ring oscillators. We report on SiNW surface treatments and dielectric ...We explore 10-nm wide Si nanowire (SiNW) field-effect transistors (FETs) for logic applications, via the fabrication and testing of SiNW-based ring oscillators. We report on SiNW surface treatments and dielectric annealing, for producing SiNW FETs that exhibit high performance in terms of large on/off-state current ratio (-10s), low drain-induced barrier lowering (-30 mV) and low subthreshold swing (-80 mV/decade). The performance of inverter and ring-oscillator circuits fabricated from these nanowire FETs are also explored. The inverter demonstrates the highest voltage gain (-148) reported for a SiNW-based NOT gate, and the ring oscillator exhibits near rail-to-rail oscillation centered at 13.4 MHz. The static and dynamic characteristics of these NW devices indicate that these SiNW-based FET circuits are excellent candidates for various high-performance nanoelectronic applications.展开更多
With the inclusion of the isovector scalar channel in the meson-nucleon couplings, taking DD-MEδ as an effective interaction, the moments of inertia of neutron stars possessing various stellar masses are studied with...With the inclusion of the isovector scalar channel in the meson-nucleon couplings, taking DD-MEδ as an effective interaction, the moments of inertia of neutron stars possessing various stellar masses are studied within the density dependent relativistic mean field (RMF) theory. The isovector scalar channel contributes to the softening of the neutron-star matter equation of state (EOS) and therefore the reduction of the maximum mass and radius of neutron stars. Smaller values of the total moment of inertia 1 and the crustal moment of inertia AI are then obtained in DD-ME~ via numerical procedure in comparison with those in other selected RMF functionals. In addition, the involvement of the isovector scalar channel lowers the thickness of the neutron star crust and its mass fi'action as well. The sensitivity to both the crustal mass and stellar radius causes the crustal moment of inertia to be more obviously reduced than the total one, eventually leading to a suppression on the fraction of crustal moment of inertia △I/I in DD-MFδ. The results indicate the crustal moment of inertia as a more sensitive probe of the neutron-star matter EOS than the total one, and demonstrate that the isovector scalar meson-nucleon couplings in the RMF theory could exert influence over the physics of pulsar glitches.展开更多
文摘To investigate the roles of maspin and kai1 expression in tumorigenesis and progression of gastric cancer. Methods Maspin and kai1 expressions were detected in normal gastric mucosa (n = 182), gastric dysplasia (n = 69), and gastric cancer (n = 113) by immunohisto-chemistry. Their expressions were compared with clinicopathological parameters of tumors. Relationship between maspin and kai1 expression was also concerned in gastric cancer. Results The positive rates of maspin expression were 79.8% (145/182), 75.4% (52/69), and 50.4% (57/113) in normal gastric mucosa, gastric dysplasia, and gastric cancer, while those of kai1 expression were 81.9% (149/182), 65.2% (49/69), and 58.4% (66/113) in corresponding tissues respectively. Gastric cancer less frequently expressed maspin than the normal gastric mucosa and gastric dysplasia (P < 0.05), while dysplasia and cancer showed less frequent expression of kai1 than normal mucosa (P < 0.05). Maspin expression showed negative association with invasive depth, metastasis, Lauren’s and histological classifications (P < 0.05), but not with tumor size, Borrmann’s classification, growth pattern or TNM staging (P > 0.05). Kai1 expression was negatively correlated with invasive depth, metastasis, growth pattern, Lauren’s and histo-logical classifications (P < 0.05), but not with tumor size, Borrmann’s classification or TNM staging (P > 0.05). Maspin and kai1 were collaboratively expressed in gastric cancer (P < 0.05). Conclusions Down-regulated expressions of maspin and kai1 play an important role in gastric carcinogenesis. Abnormal expression of maspin and kai1 might have inhibitory effects on invasion and metastasis of gastric cancer and act as an effe-ctive and objective marker to indicate the pathobiological behaviors of gastric cancer.
文摘Long-term field experiment was established in 1978 on a coastal paddy soil to determine the effect of applicationof pig manure, rice straw and chemical N fertilizer on the physical property and humus characteristics of soil . Resultsshowed that the porosity, the microstructural coefficient, the reactivities of organic C and N, the ΔlogK value, thedegree of oxidation stability, the contents of O-alkyl C and alkyl C, and the ratio of aliphatic C to aromatic C ofhumic acid from soils received organic manure increased, whereas, the ratio of < 10 μm to >10 μm ofmicroaggregates, the humification degree of humus, the degree of organo-mineral complexation, the number-averagemolecular weight, the C/ H ratio. the contents of carboxyl and aromatic C of HAs in them decreased. These resultsindicated that the application of organic manure not only improved the physical property of the paddy soil but alsomade the HA more aliphatic in structure and younger in origin.
文摘Nowadays, Computer Assisted Instruction is widespread in universities while the teaching effect is far from satisfying. Therefore. it is necessary to explore the current situation of Application of Computer Assisted Instruction, analyze irreplaceable advantages as well as causes of ineffectiveness in teaching, and and bring forward the approach to solving them.
文摘Purpose: This paper is a report about the concept analysis of continuity of care, to get further understanding of continuity of care. Methods: The Walker and Avant concept analysis approach was applied in this paper. Results: The three attributes include: seamless transfer, flexible reaction and effective interaction. The antecedents were changed needs and patient centred care. In addition, the continuity of care had a significant effect on patients’ outcome, satisfaction and costs. Conclusions: This analysis provides nurses an understanding about continuity of care in both patient’s and care provider’s perspective. Continuity of care is a tailored services provided by care givers, which is an essential element of healthcare system.
文摘The presented work is based on laboratory testing of seed germination speed, emergence and seedling growth under different stress conditions simulated by subnormal water revel, extreme high and low temperatures. It allows one to eliminate the plant materials (initial breeding materials and cultivars) which do not tolerate extreme temperatures and temperature changes during germination, have low water use efficiency and is intolerant to abiotic stressors all right at the seed level. It was confirmed that these genotypes also have poor t^eld emergence and initial root growth implications for further vegetation periods, mainly for over wintering and spring regeneration which has significant influence on the yield. The method also represents the tool for screening genetic resources with the resistance to the abiotic stressors and this technology process is also acceptable for other crops. The results confirm the importance of the seed and root characteristics for crop production. The deteriorating quality of soil in recent years, increasing variability of weather and long periods of drought directly support the need to intensify activities in this research. Obtained results also show the influence of seed quality characteristics on roots and above ground parts of the plant. A relationship exists also vice versa.
基金supported jointly by Chinese Ministry of Science and Technology (Grant 2005DIB3J099)Science Foundation of Shaanxi Province (Grant 2007D11)
文摘A case of hailstorm process occurring on 24 June 2006 in northwestern China was studied using satellite retrieval methodology. The particle effective radius (re) in the cloud tops was calculated by the reflectance in the 3.7 μm channel, and cloud-top microphysical properties were vividly represented using the RGB visual multispectral classification scheme. The microphysical zones of clouds and the processes of hail formation and develop-ment are inferred using the relations of cloud-top temperature (T) versus re for the tops of convective clouds. The results show that particle effective radius was smaller near the cloud base of hailstorm. There was a deep zone of diffusional droplet growth at the low level where the particles grew slowly with height, and there existed an evident area of small ice particles in the cloud top, suggesting the existence of a strong updraft in the clouds. The low glaciated temperature indicated a great depth from the cloud base to the glaciation height, which provided a deep layer of supercooled water for hail growth.
基金the Natural Science Foundation of Henan Province(182300410053)the China Postdoctoral Science Foundation(2020M672308)+3 种基金Henan Postdoctoral Science Foundation(227462)Science Project(902019AA0140)the National Key Research and Development Program of China(2019YFA0905100)the National Natural Science Foundation of China(U2004143)。
文摘Restricted genetic diversity can supply only a limited number of elite genes for modern plant cultivation and transgenesis.In this study,we demonstrate that rational design enables the engineering of geranyl-geranyl diphosphate synthase(NtGGPPS),an enzyme of the methylerythritol phosphate pathway(MEP)in the model plant Nicotiana tabacum.As the crucial bottleneck in carotenoid biosynthesis,NtGGPPS1 interacts with phytoene synthase(NtPSY1)to channel GGPP into the production of carotenoids.Loss of this enzyme in the ntggpps1 mutant leads to decreased carotenoid accumulation.With the aim of enhanc-ing NtGGPPS1 activity,we undertook structure-guided rational redesign of its substrate binding pocket in combination with sequence alignment.The activity of the designed NtGGPPS1(a pentuple mutant of five sites V154A/I161L/F218Y/I209S/V233E,d-NtGGPPS1)was measured by a high-throughput colorimetric assay.d-NtGGPPS1 exhibited significantly higher conversion of IPP and each co-substrate(DMAPP~1995.5-fold,GPP~25.9-fold,and FPP~16.7-fold)for GGPP synthesis compared with wild-type NtGGPPS1.Importantly,the transient and stable expression of d-NtGGPPS1 in the ntggpps1 mutant increased carotenoid levels in leaves,improved photosynthetic efficiency,and increased biomass relative to NtGGPPS1.These findings provide a firm basis for the engineering of GGPPS and will facilitate the development of quality and yield traits.Our results open the door for the structure-guided rational design of elite genes in higher plants。
文摘This paper tries to find interaction rules between members and self-reform rules of members in some scientific research laboratories at a graduate school. The candidate of rules are extracted from the personality description sentences about factors: extroversion, agreeableness, conscientiousness, neuroticism and openness in the big five theory of personality psychology. In this paper, interaction and self-reform rules are not described by personalities themselves; instead, they are described by corresponding phenomena: activity, empathy, persistence, autonomy, and analytical ability which appear in the behavior side. Each member's initial value is determined from the main 5-factor personality investigation to the member, and the target value is given by the member's self-declaration. The rule selection is carried out by the genetic algorithm, where a sigmoid function is introduced in the renewal algorithm of the value, whereby a difficulty of setting the renewal parameters is avoided. An analysis is added about the obtained rules, and the validity of this approach and the subjects for future study are discussed. Finally, consideration is extended to the effect of environment on the behavior of members to reinforce the set of rules.
基金The authors acknowledge H. Ahmad and Y. -S. Shin for graphics assistance. This work was funded by the National Science Foundation under Grant CCF-0541461 and the Department of Energy (DE-FG02-04ER46175). D. Tham gratefully acknowledges support by the KAUST Scholar Award.
文摘We explore 10-nm wide Si nanowire (SiNW) field-effect transistors (FETs) for logic applications, via the fabrication and testing of SiNW-based ring oscillators. We report on SiNW surface treatments and dielectric annealing, for producing SiNW FETs that exhibit high performance in terms of large on/off-state current ratio (-10s), low drain-induced barrier lowering (-30 mV) and low subthreshold swing (-80 mV/decade). The performance of inverter and ring-oscillator circuits fabricated from these nanowire FETs are also explored. The inverter demonstrates the highest voltage gain (-148) reported for a SiNW-based NOT gate, and the ring oscillator exhibits near rail-to-rail oscillation centered at 13.4 MHz. The static and dynamic characteristics of these NW devices indicate that these SiNW-based FET circuits are excellent candidates for various high-performance nanoelectronic applications.
基金supported by the National Natural Science Foundation of China(Grant No.11375076)the Fundamental Research Funds for the Central Universities(Grant No.lzujbky-2016-30)
文摘With the inclusion of the isovector scalar channel in the meson-nucleon couplings, taking DD-MEδ as an effective interaction, the moments of inertia of neutron stars possessing various stellar masses are studied within the density dependent relativistic mean field (RMF) theory. The isovector scalar channel contributes to the softening of the neutron-star matter equation of state (EOS) and therefore the reduction of the maximum mass and radius of neutron stars. Smaller values of the total moment of inertia 1 and the crustal moment of inertia AI are then obtained in DD-ME~ via numerical procedure in comparison with those in other selected RMF functionals. In addition, the involvement of the isovector scalar channel lowers the thickness of the neutron star crust and its mass fi'action as well. The sensitivity to both the crustal mass and stellar radius causes the crustal moment of inertia to be more obviously reduced than the total one, eventually leading to a suppression on the fraction of crustal moment of inertia △I/I in DD-MFδ. The results indicate the crustal moment of inertia as a more sensitive probe of the neutron-star matter EOS than the total one, and demonstrate that the isovector scalar meson-nucleon couplings in the RMF theory could exert influence over the physics of pulsar glitches.