On the basis of ecological principles including holistic optimization, cycling and regeneration, and regional differentiation, land treatment systems (LTSs) for municipal wastewater were continuously explored and upda...On the basis of ecological principles including holistic optimization, cycling and regeneration, and regional differentiation, land treatment systems (LTSs) for municipal wastewater were continuously explored and updated in the western Shenyang area and the Huolinhe area, China. Intensified pretreatment, addition of a man-made soil filtration layer, and use of an ecologically diversified secondary plant cover were proved to be technically feasible. Hydraulic loading was determined according to the assimilation capacity of soil ecosystems, thus ensuring safe operation of wastewater treatment. This modernized and alternative approach to wastewater treatment had been widely applied in middle-sized and small cities and towns of Northeast China, and these innovative systems in some areas had indicated favorable ecological, social, and economic benefits.展开更多
One influenza H3N2 virus, A/swine/Shandong/3/2005 (Sw/SD/3/2005), was isolated from pigs with respiratory disease on a farm in eastern China. Genetic analysis revealed that Sw/SD/3/2005 was a triple-reassortant virus ...One influenza H3N2 virus, A/swine/Shandong/3/2005 (Sw/SD/3/2005), was isolated from pigs with respiratory disease on a farm in eastern China. Genetic analysis revealed that Sw/SD/3/2005 was a triple-reassortant virus with a PB2 gene from human-like H1N1, NS from classical swine H1N1, and the remaining genes from human-like H3N2 virus. These findings further support the concept that swine can serve as reservoir or mixing vessels of influenza virus strains and maintain genetic and antigenic stability of viruses. Furthermore, we have successfully established a reverse genetics system based on eight plasmids and rescued Sw/SD/3/2005 through cell transfection. HI tests and RT-PCR confirmed that the rescued virus maintained the biological properties of the wild type Sw/SD/3/2005. The successful establishment of the reverse genetics system of Sw/SD/3/2005 will enable us to conduct extensive studies of the molecular evolution of H3N2 influenza viruses in swine.展开更多
this paper mainly discusses the design of distributed effluenttreatment systems with single contami- nant. A new method is putforward and four basic rules are provided. The key point of themethod is that global optima...this paper mainly discusses the design of distributed effluenttreatment systems with single contami- nant. A new method is putforward and four basic rules are provided. The key point of themethod is that global optimality is obtained by guaranteeing theoptimality of each step taken in the design. Costs per unit mass ofremoved contaminant are used as a scale to choose the nextcombination of an effluent stream and a treatment process. Theremaining problem is updated after each choice. As for multiplecontaminants, a two-stage method is adopted.展开更多
Remote sensing techniques and geographic information systems offer a good means of collecting and manipulating the data required for assessing conservation practices. A method for automatic generation of most of the d...Remote sensing techniques and geographic information systems offer a good means of collecting and manipulating the data required for assessing conservation practices. A method for automatic generation of most of the data required by the Agricultural Non point Source erosion model is developed from three sources:(1) file with contour lines from the topographic maps(TM), (2) soil mapping units from the soil surveys, and (3)land cover from land sat TM image classification.展开更多
A sequential three-step programming method is proposed for determining the minimum flowrate of fresh water and corresponding regenerated water in water-using system of single contaminant with regeneration reuse. In st...A sequential three-step programming method is proposed for determining the minimum flowrate of fresh water and corresponding regenerated water in water-using system of single contaminant with regeneration reuse. In step 1, a programming with the objective of min fws is used to determine the minimum flowrate of fresh water, in which the mathematical representation is a mixed integer nonlinear programming (MINLP1). Then under the same constraints with step 1, a programming with the objective of min freg in step 2 and a programming with the objective of min Cr in step 3 are subsequently used to determine the minimum flowrate of regenerated water and the minimum inlet concentration to regeneration process corresponding to the minimum flowrate of fresh water based on step 1. The method is easy to apply because we only need to change the objective function but keep the constraints constant to go along the following steps after step 1. In addition, the relationship between the fresh water flowrate required, fws and inlet concentration to regeneration process, Cr, is investigated. It is found that there exist three relationships between fws and Cr, which indicate three possibilities for C\>: below the pinch, above the pinch or at the pinch. Therefore, a new conclusion is drawn, which differs from that 'regeneration of water at pinch minimizes fresh water flowrate' derived in literature and indicates that in some cases, regeneration at other point also minimizes fresh water flowrate.展开更多
Coral reefs in the Negril Marine Park (NMP), Jamaica, have been increasingly impacted by nutrient pollution and macroalgal blooms following decades of intensive development as a major tourist destination. A baseline...Coral reefs in the Negril Marine Park (NMP), Jamaica, have been increasingly impacted by nutrient pollution and macroalgal blooms following decades of intensive development as a major tourist destination. A baseline survey of DIN and SRP concentrations, C:N:P and stable nitrogen isotope ratios (δ^15N) of abundant reef macroalgae on shallow and deep reefs of the NMP in 1998 showed strong P-limitation and evidence of increasing sewage pollution. In 1999, a sewage collection and treatment project began diverting wastewater from the resort and urban areas to a pond system that discharged partially-treated effluent into the South Negril River (SNR). These sewage discharges significantly increased concentrations of NH2 and SRP (N:P -13) in the SNR, which flows into Long Bay and around Negril's "West End". Concentrations of SRP, the primary limiting nutrient, were higher on shallow reefs of the West End in 2001 compared to 1998. Stable nitrogen isotope ratios (δ^15N) of abundant reef macroalgae on both shallow and deep reefs of the West End in 2002 were significantly higher than baseline values in 1998, indicating an escalating impact of sewage nitrogen pollution over this timeframe. The increased nutrient concentrations and δ^15N enrichment of reef macroalgae correlated with blooms of the chlorophyte Chaetornorpha linum in shallow waters of Long Bay and Codium isthrnocladum and Caulerpa cupressoides on deep reefs of the West End. Sewage treatment systems adjacent to coral reefs must include nutrient removal to ensure that DIN and SRP concentrations, after dilution, are below the low thresholds noted for these oligotrophic ecosystems.展开更多
Diatomite is a kind of natural low-cost mineral material. It has a number of unique physical properties and has been widely used as an adsorbent in wastewater treatment. This study was conducted to investigate the aer...Diatomite is a kind of natural low-cost mineral material. It has a number of unique physical properties and has been widely used as an adsorbent in wastewater treatment. This study was conducted to investigate the aerobic biodegradation of coal gasification wastewater with and without diatomite addition. Experimental results indicated that diatomite added in the activated sludge system could promote the biomass and also enhance the performance of the sludge settling. The average mixed-liquor volatile suspended solids (MLVSS) is increased from 4055 mg.L^-1 to 4518 mg.L^-1 and the average settling volume (SV) are changed only from 45.9% to 47.1%. Diatomite additive could enhance the efficiency of chemical oxygen demand (COD) and total phenols removal from the wastewater. The COD removal increased from 73.3% to near 80% and the total phenols removal increased from 81.4% to 85.8%. The mechanisms of the increase of biomass and pollutants removal may correlates to the improvement of bioavailability and sludge settlement characteristics by diatomite added. Micrograph of the sludge in the diatomite-activated sludge system indicated that the diatomite added could be the carrier of the microbe and also affect the biomass and pollutant removal.展开更多
Biofilm-associated microorganisms play crucial roles in terrestrial and aquatic nutrient cycling and in the biodegradation of environmental pollutants. Biofilm formation was determined for a total of 18 bacterial isol...Biofilm-associated microorganisms play crucial roles in terrestrial and aquatic nutrient cycling and in the biodegradation of environmental pollutants. Biofilm formation was determined for a total of 18 bacterial isolates obtained from the biofilms of wastewater treatment systems and of little carpolite in soil. Among these isolates, seven showed strong biofilm-forming capacity. The phylogenetic affiliation of the isolates showing high biofilm formation capacity was determined through 16S rDNA sequencing and the isolates were grouped into 7 bacterial species including Pseudornonas sp., Pseudomonas putida, Aeromonas caviae, Bacillus cereus, Pseudornonas plecoglossicida, Aeromonas hydrophila, and Comamonas testosteroni. The biofilm-forming capacity was closely related with flagella, exopolysaccharide, and extracellular protein. According to the coefficient of determination, the relative importance of the five biological characteristics to biofilm formation was, in order from greatest to least, exopolysaccharide 〉 flagella 〉 N-acyl-homoserine lactones (AHLs) signaling molecules 〉 extracellular protein 〉 swarming motility.展开更多
CO2 removal from biogas by water washing system was investigated with various parameters, including liquid/ gas ratio, pressure, temperature, and CO2 content. The results indicate that CO2 removal ratio could reach 34...CO2 removal from biogas by water washing system was investigated with various parameters, including liquid/ gas ratio, pressure, temperature, and CO2 content. The results indicate that CO2 removal ratio could reach 34.6%- 94.2% as liquid/gas ratio increased from 0.14 to 0.50. Increasing pressure (from 0.8 to 1.2 MPa) could improve gas purification with a constant inflow rate of gas. Temperature played a key role in the process and lower temper- ature in absorption tower was beneficial for reducing CO2 content. CO2 removal ratio could reach 24.4%-83.2% when CO2 content in the simulated gas was 25%-45%. The lowest CO2 content after absorption was 2.6% at 1.2 MPa with 400 L·h-1 gas flow and 200 L·h-1 water flow, which meets the requirement of CO2 content in natural Ras for vehicle fuel.展开更多
In this study, we evaluated the effects of CO_2-induced seawater acidification on fertilization, embryogenesis and early larval development in the sea urchin Glyptocidaris crenularis, that inhabits subtidal coastal ar...In this study, we evaluated the effects of CO_2-induced seawater acidification on fertilization, embryogenesis and early larval development in the sea urchin Glyptocidaris crenularis, that inhabits subtidal coastal areas in northern China. The range in seawater p H used in experiments was based on the projections of the Intergovernmental Panel on Climate Change(IPCC), to the year 2100. A natural seawater treatment(p H_(nbs) =7.98±0.03) and three laboratory-controlled acidified treatments(OA_1, ΔpH_(nbs) =-0.3 units; OA_2, ΔpH_(nbs) =-0.4 units; OA_3, ΔpH_(nbs) =-0.5 units) were used in experiments. Results show that:(1) there was a negative effect of seawater acidification on fertilization and on the percentage of abnormal fertilized eggs;(2) the size of early cleavage stage embryos decreased in a dose-dependent manner with decreasing p H;(3) both the hatching rate of blastulae and the survival rate of four-armed pluteus larvae decreased as pH declined;(4) larval abnormalities including asymmetrical development, changes in the length of skeletal elements, and corroded spicules were observed in all seawater acidified-treatments compared with the control. These data indicate that seawater acidification has a negative impact on the early development of G. crenularis, and supports the hypothesis that the response of echinoderms to ocean acidification(OA) varies among species. Further research is required to clarify the specific cellular mechanisms involved.展开更多
Water leaving the treatment works is usually of a high quality but its properties change during the transportation stage. Increasing awareness of the quality of the service provided within the water industry today and...Water leaving the treatment works is usually of a high quality but its properties change during the transportation stage. Increasing awareness of the quality of the service provided within the water industry today and assessing the reliability of the water quality in a distribution system has become a major significance for decision on system operation based on water quality in distribution networks. Using together a water age model, a chlorine decay model and a model of acceptable maximum water age can assess the reliability of the water quality in a distribution system. First, the nodal water age values in a certain complex distribution system can be calculated by the water age model. Then, the acceptable maximum water age value in the distribution system is obtained based on the chlorine decay model. The nodes at which the water age values are below the maximum value are regarded as reliable nodes. Finally, the reliability index on the percentile weighted by the nodal demands reflects the reliability of the water quality in the distribution system. The approach has been applied in a real water distribution network. The contour plot based on the water age values determines a surface of the reliability of the water quality. At any time, this surface is used to locate high water age but poor reliability areas, which identify parts of the network that may be of poor water quality. As a result, the contour water age provides a valuable aid for a straight insight into the water quality in the distribution system.展开更多
This paper deals with a new system of aquaculture, i.e., a closed recirculating system for shrimp-mollusk polyculture. The culture system consisted of several shrimp ponds, a mollusk water-purifying pond and a reservo...This paper deals with a new system of aquaculture, i.e., a closed recirculating system for shrimp-mollusk polyculture. The culture system consisted of several shrimp ponds, a mollusk water-purifying pond and a reservoir. During the production cycle, water circulated between the shrimp and mollusk ponds, and the reservoir compensated for water loss from seepage and evaporation. Constricted tagelus, Sinonovacula con- stricta, was selected as the cultured mollusk, and Pacific white shrimp, Litopenaeus vannamei, as the cultured shrimp. The main managing measures during the production cycle were: setting and using the aerators; introduc- ing the probiotic products timely into the shrimp ponds; adopting a "pen-closing" method for controlling shrimp viral epidemics; setting the flow diversion barriers in the mollusk pond to keep the circulating water flowing through the pond along a sine-like curve and serve as substrate for biofilm; no direct feeding was necessary for the cultured mollusk until the co-cultured shrimp was harvested; natural foods in the water from the shrimp ponds was used for their foods. Two sets of the system were used in the experiment in 2002 and satisfactory re- sults were achieved. The average yield of the shrimp was 11 943.5 kg/hm2, and that of the mollusk was 16 965 kg/hm2. After converting the mollusk yield into shrimp yield at their market price ratio, the food coefficient of the entire system averaged at as low as 0.81. The water quality in the ponds was maintained at a desirable level and no viral epidemics were discovered during the production cycle.展开更多
Groundwater is one of the most important resources, its monitoring and optimized management has now become the priority to satisfy the demand of rapidly increasing population. In many developing countries, optimized g...Groundwater is one of the most important resources, its monitoring and optimized management has now become the priority to satisfy the demand of rapidly increasing population. In many developing countries, optimized groundwater level monitoring networks are rarely designed to build up a strong groundwater level data base, and to reduce operation time and cost. The paper presents application of geostatistical method to optimize existing network of observation wells for 18 sub-watersheds within the Wainganga Sub-basin located in the central part of India. The average groundwater level fluctuation(GWLF) from 37 observation wells is compared with parameters like lineament density, recharge, density of irrigation wells, land use and hydrogeology(LiRDLH) of Wainganga Sub-basin and analyzed stochastically in Geographic Information System(GIS) environment using simple, ordinary, disjunctive and universal kriging methods. Semivariogram analyses have been performed separately for all kriging methods to fit the best theoretical model with experimental model. Results from gaussian, spherical, exponential and circular theoretical models were compared with those of experimental models obtained from the groundwater level data. Spatial analyses conclude that the exponential semivariogram model obtained from ordinary kriging gives the best fit model. Study demonstrates that ordinary kriging gives the optimal solution and additional number of observation wells can be added utilizing the error variance for optimal design of groundwater level monitoring networks. This study describes the use of Geostatistics methods in GIS to predict the groundwater level and upgrade groundwater level monitoring networks from the randomly distributed observation wells considering multiple parameters such as GWLF and LiRDLH. The method proposed in the present study is observed to be an efficient method for selecting observation well locations in a complex geological set up. The study concludes that minimum 82 wells are required for proper monitoring of groundwater level in the study area.展开更多
Peritrich ciliates are highly diverse and can be important bacterial grazers in aquatic ecosystems. Morphological identifi cations of peritrich species and assemblages in the environment are time-consuming and experti...Peritrich ciliates are highly diverse and can be important bacterial grazers in aquatic ecosystems. Morphological identifi cations of peritrich species and assemblages in the environment are time-consuming and expertise-demanding. In this study, two peritrich-specifi c PCR primers were newly designed to amplify a fragment including the internal transcribed spacer(ITS) region of ribosomal rDNA from environmental samples. The primers showed high specifi city in silico, and in tests with peritrich isolates and environmental DNA. Application of these primers in clone library construction and sequencing yielded exclusively sequences of peritrichs for water and sediment samples. We also found the ITS1, ITS2, ITS, D1 region of 28 S rDNA, and ITS+D1 region co-varied with, and generally more variable than, the V9 region of 18 S rDNA in peritrichs. The newly designed specifi c primers thus provide additional tools to study the molecular diversity, community composition, and phylogeography of these ecologically important protists in dif ferent systems.展开更多
Miyun Reservoir was designated as the water source of Beijing City in 1982. Since that time, socio economic development in Miyun Area has been slowing due to the restriction of severe environmental standards. More and...Miyun Reservoir was designated as the water source of Beijing City in 1982. Since that time, socio economic development in Miyun Area has been slowing due to the restriction of severe environmental standards. More and more attention from the public and government has been paid to the regional sustainable development. And an effective planning for the local society management system is urgently desired. In this study, a regional sustainable development system dynamics model, named MiyunSD, is developed for supporting this planning task. MiyunSD consists of dynamic simulation models that explicitly consider information feedback that governs interactions in the system. Such models are capable of simulating the system′s behavior and predicting its developing situation of the future. For the study case, interactions among a number of system components within a time frame of fifteen years are examined dynamically. Three planning alternatives are carefully considered. The base run is based on an assumption that the existing pattern of human activities will prevail in the entire planning horizon, and the other alternatives are based on previous and present planning studies. The different alternatives will get different system′s environmental and socio economic results. Through analyzing these dynamic results, local authorities may find an optimal way to realize the objectives that the regional environment will be well protected and at the same time the economy will be rapidly developed.展开更多
Tunnels in an urban area, in many cases, are constructed in soft ground which contains underground water, near existing facilities and structures. Structural stability for the tunnel and also the nearby structures and...Tunnels in an urban area, in many cases, are constructed in soft ground which contains underground water, near existing facilities and structures. Structural stability for the tunnel and also the nearby structures and facilities is vital in this kind of work. Slurry pipe jacking was firmly established as a special method for the non-disruptive construction of underground pipelines of sewage systems. This method utilizes mud slurry that is formed around the pipes in order to stabilize the surrounding soil. In the pipe roof method the tubing elements that are constructed by slurry pipe jacking are near each other longitudinally, and create a rigid and stable lining before the excavation of the main tunnel. This paper discusses 'the application of a slurry pipe jacking system on 'the pipe roof method by means of numerical analysis. Because of the rigid behavior of the lining, the results show little subsidence, making this method a reliable method of constructing large tunnels with small cover in an urban area.展开更多
High salinity industrial wastewater is difficult to treat using biological treatment system because of the high concentrations of salt.The potential of a sequencing batch biofilm reactor(SBBR)process in treating synth...High salinity industrial wastewater is difficult to treat using biological treatment system because of the high concentrations of salt.The potential of a sequencing batch biofilm reactor(SBBR)process in treating synthetic high salinity wastewater was evaluated at laboratory scale during a 110-day operation.The reactor was operated in a 12 h cycle,and each cycle consisted of 0.25 h influent addition,8 h aeration,3 h anoxic reaction,0.5 h sedimentation and 0.25 h effluent withdrawal.Gradual increase in salinity gradient was applied during the acclimatization period.The acclimated SBBR system was demonstrated to be an effective process to remove organic compounds and ammonia nitrogen under high salinity conditions with chemical oxygen demand(COD)and ammonia nitrogen(NH3-N)removal efficiencies of 88% and 80%,respectively.The microscopic examination indicated that rather than rotifers or vorticella,the zoogloea,filamentous fungus mingled with a small quantity of swimming infusorians were dominant bacteria in SBBR system.The removal efficiencies close to 80% in COD and 75% in NH3-N were achieved at an organic loading rate(OLR)of 0.96 kg COD/(m3·d),pH of 7.0,salinity of 14 g/L and NH3-N of 30 mg/L.展开更多
基金Project supported by the National Natural Science Founds for Distinguished Young Scholars, China (No. 20225722)the Key Programme of the National Natural Science Foundation of China (No. 20337010)
文摘On the basis of ecological principles including holistic optimization, cycling and regeneration, and regional differentiation, land treatment systems (LTSs) for municipal wastewater were continuously explored and updated in the western Shenyang area and the Huolinhe area, China. Intensified pretreatment, addition of a man-made soil filtration layer, and use of an ecologically diversified secondary plant cover were proved to be technically feasible. Hydraulic loading was determined according to the assimilation capacity of soil ecosystems, thus ensuring safe operation of wastewater treatment. This modernized and alternative approach to wastewater treatment had been widely applied in middle-sized and small cities and towns of Northeast China, and these innovative systems in some areas had indicated favorable ecological, social, and economic benefits.
文摘One influenza H3N2 virus, A/swine/Shandong/3/2005 (Sw/SD/3/2005), was isolated from pigs with respiratory disease on a farm in eastern China. Genetic analysis revealed that Sw/SD/3/2005 was a triple-reassortant virus with a PB2 gene from human-like H1N1, NS from classical swine H1N1, and the remaining genes from human-like H3N2 virus. These findings further support the concept that swine can serve as reservoir or mixing vessels of influenza virus strains and maintain genetic and antigenic stability of viruses. Furthermore, we have successfully established a reverse genetics system based on eight plasmids and rescued Sw/SD/3/2005 through cell transfection. HI tests and RT-PCR confirmed that the rescued virus maintained the biological properties of the wild type Sw/SD/3/2005. The successful establishment of the reverse genetics system of Sw/SD/3/2005 will enable us to conduct extensive studies of the molecular evolution of H3N2 influenza viruses in swine.
文摘this paper mainly discusses the design of distributed effluenttreatment systems with single contami- nant. A new method is putforward and four basic rules are provided. The key point of themethod is that global optimality is obtained by guaranteeing theoptimality of each step taken in the design. Costs per unit mass ofremoved contaminant are used as a scale to choose the nextcombination of an effluent stream and a treatment process. Theremaining problem is updated after each choice. As for multiplecontaminants, a two-stage method is adopted.
文摘Remote sensing techniques and geographic information systems offer a good means of collecting and manipulating the data required for assessing conservation practices. A method for automatic generation of most of the data required by the Agricultural Non point Source erosion model is developed from three sources:(1) file with contour lines from the topographic maps(TM), (2) soil mapping units from the soil surveys, and (3)land cover from land sat TM image classification.
基金Supported by the National Fundamental Research Development Program of China(No.2000026308).
文摘A sequential three-step programming method is proposed for determining the minimum flowrate of fresh water and corresponding regenerated water in water-using system of single contaminant with regeneration reuse. In step 1, a programming with the objective of min fws is used to determine the minimum flowrate of fresh water, in which the mathematical representation is a mixed integer nonlinear programming (MINLP1). Then under the same constraints with step 1, a programming with the objective of min freg in step 2 and a programming with the objective of min Cr in step 3 are subsequently used to determine the minimum flowrate of regenerated water and the minimum inlet concentration to regeneration process corresponding to the minimum flowrate of fresh water based on step 1. The method is easy to apply because we only need to change the objective function but keep the constraints constant to go along the following steps after step 1. In addition, the relationship between the fresh water flowrate required, fws and inlet concentration to regeneration process, Cr, is investigated. It is found that there exist three relationships between fws and Cr, which indicate three possibilities for C\>: below the pinch, above the pinch or at the pinch. Therefore, a new conclusion is drawn, which differs from that 'regeneration of water at pinch minimizes fresh water flowrate' derived in literature and indicates that in some cases, regeneration at other point also minimizes fresh water flowrate.
文摘Coral reefs in the Negril Marine Park (NMP), Jamaica, have been increasingly impacted by nutrient pollution and macroalgal blooms following decades of intensive development as a major tourist destination. A baseline survey of DIN and SRP concentrations, C:N:P and stable nitrogen isotope ratios (δ^15N) of abundant reef macroalgae on shallow and deep reefs of the NMP in 1998 showed strong P-limitation and evidence of increasing sewage pollution. In 1999, a sewage collection and treatment project began diverting wastewater from the resort and urban areas to a pond system that discharged partially-treated effluent into the South Negril River (SNR). These sewage discharges significantly increased concentrations of NH2 and SRP (N:P -13) in the SNR, which flows into Long Bay and around Negril's "West End". Concentrations of SRP, the primary limiting nutrient, were higher on shallow reefs of the West End in 2001 compared to 1998. Stable nitrogen isotope ratios (δ^15N) of abundant reef macroalgae on both shallow and deep reefs of the West End in 2002 were significantly higher than baseline values in 1998, indicating an escalating impact of sewage nitrogen pollution over this timeframe. The increased nutrient concentrations and δ^15N enrichment of reef macroalgae correlated with blooms of the chlorophyte Chaetornorpha linum in shallow waters of Long Bay and Codium isthrnocladum and Caulerpa cupressoides on deep reefs of the West End. Sewage treatment systems adjacent to coral reefs must include nutrient removal to ensure that DIN and SRP concentrations, after dilution, are below the low thresholds noted for these oligotrophic ecosystems.
基金Supported by the Shanghai Committee of Education (07ZZ158)
文摘Diatomite is a kind of natural low-cost mineral material. It has a number of unique physical properties and has been widely used as an adsorbent in wastewater treatment. This study was conducted to investigate the aerobic biodegradation of coal gasification wastewater with and without diatomite addition. Experimental results indicated that diatomite added in the activated sludge system could promote the biomass and also enhance the performance of the sludge settling. The average mixed-liquor volatile suspended solids (MLVSS) is increased from 4055 mg.L^-1 to 4518 mg.L^-1 and the average settling volume (SV) are changed only from 45.9% to 47.1%. Diatomite additive could enhance the efficiency of chemical oxygen demand (COD) and total phenols removal from the wastewater. The COD removal increased from 73.3% to near 80% and the total phenols removal increased from 81.4% to 85.8%. The mechanisms of the increase of biomass and pollutants removal may correlates to the improvement of bioavailability and sludge settlement characteristics by diatomite added. Micrograph of the sludge in the diatomite-activated sludge system indicated that the diatomite added could be the carrier of the microbe and also affect the biomass and pollutant removal.
基金supported by the National Natural Science Foundation of China (No.30600016)the Environment Protection Department of Jiangsu Province,China (No.2004007)
文摘Biofilm-associated microorganisms play crucial roles in terrestrial and aquatic nutrient cycling and in the biodegradation of environmental pollutants. Biofilm formation was determined for a total of 18 bacterial isolates obtained from the biofilms of wastewater treatment systems and of little carpolite in soil. Among these isolates, seven showed strong biofilm-forming capacity. The phylogenetic affiliation of the isolates showing high biofilm formation capacity was determined through 16S rDNA sequencing and the isolates were grouped into 7 bacterial species including Pseudornonas sp., Pseudomonas putida, Aeromonas caviae, Bacillus cereus, Pseudornonas plecoglossicida, Aeromonas hydrophila, and Comamonas testosteroni. The biofilm-forming capacity was closely related with flagella, exopolysaccharide, and extracellular protein. According to the coefficient of determination, the relative importance of the five biological characteristics to biofilm formation was, in order from greatest to least, exopolysaccharide 〉 flagella 〉 N-acyl-homoserine lactones (AHLs) signaling molecules 〉 extracellular protein 〉 swarming motility.
基金Supported by the National Technology Research and Development Program of China(2008AA062402)the China-US International Cooperation Project(2011DFA90800)the Ministry of Science and Technology,China
文摘CO2 removal from biogas by water washing system was investigated with various parameters, including liquid/ gas ratio, pressure, temperature, and CO2 content. The results indicate that CO2 removal ratio could reach 34.6%- 94.2% as liquid/gas ratio increased from 0.14 to 0.50. Increasing pressure (from 0.8 to 1.2 MPa) could improve gas purification with a constant inflow rate of gas. Temperature played a key role in the process and lower temper- ature in absorption tower was beneficial for reducing CO2 content. CO2 removal ratio could reach 24.4%-83.2% when CO2 content in the simulated gas was 25%-45%. The lowest CO2 content after absorption was 2.6% at 1.2 MPa with 400 L·h-1 gas flow and 200 L·h-1 water flow, which meets the requirement of CO2 content in natural Ras for vehicle fuel.
基金Supported by the National Natural Science Foundation of China(No.41206128)the Program for Liaoning Excellent Talents in University(No.LJQ2013079)the National High Technology Research and Development Program of China(863 Program)(No.2012AA10A412)
文摘In this study, we evaluated the effects of CO_2-induced seawater acidification on fertilization, embryogenesis and early larval development in the sea urchin Glyptocidaris crenularis, that inhabits subtidal coastal areas in northern China. The range in seawater p H used in experiments was based on the projections of the Intergovernmental Panel on Climate Change(IPCC), to the year 2100. A natural seawater treatment(p H_(nbs) =7.98±0.03) and three laboratory-controlled acidified treatments(OA_1, ΔpH_(nbs) =-0.3 units; OA_2, ΔpH_(nbs) =-0.4 units; OA_3, ΔpH_(nbs) =-0.5 units) were used in experiments. Results show that:(1) there was a negative effect of seawater acidification on fertilization and on the percentage of abnormal fertilized eggs;(2) the size of early cleavage stage embryos decreased in a dose-dependent manner with decreasing p H;(3) both the hatching rate of blastulae and the survival rate of four-armed pluteus larvae decreased as pH declined;(4) larval abnormalities including asymmetrical development, changes in the length of skeletal elements, and corroded spicules were observed in all seawater acidified-treatments compared with the control. These data indicate that seawater acidification has a negative impact on the early development of G. crenularis, and supports the hypothesis that the response of echinoderms to ocean acidification(OA) varies among species. Further research is required to clarify the specific cellular mechanisms involved.
文摘Water leaving the treatment works is usually of a high quality but its properties change during the transportation stage. Increasing awareness of the quality of the service provided within the water industry today and assessing the reliability of the water quality in a distribution system has become a major significance for decision on system operation based on water quality in distribution networks. Using together a water age model, a chlorine decay model and a model of acceptable maximum water age can assess the reliability of the water quality in a distribution system. First, the nodal water age values in a certain complex distribution system can be calculated by the water age model. Then, the acceptable maximum water age value in the distribution system is obtained based on the chlorine decay model. The nodes at which the water age values are below the maximum value are regarded as reliable nodes. Finally, the reliability index on the percentile weighted by the nodal demands reflects the reliability of the water quality in the distribution system. The approach has been applied in a real water distribution network. The contour plot based on the water age values determines a surface of the reliability of the water quality. At any time, this surface is used to locate high water age but poor reliability areas, which identify parts of the network that may be of poor water quality. As a result, the contour water age provides a valuable aid for a straight insight into the water quality in the distribution system.
基金This work was supported by the Science and Technology Commissionof Ningbo under the grant No. Yong-ke-ji: [2001]235
文摘This paper deals with a new system of aquaculture, i.e., a closed recirculating system for shrimp-mollusk polyculture. The culture system consisted of several shrimp ponds, a mollusk water-purifying pond and a reservoir. During the production cycle, water circulated between the shrimp and mollusk ponds, and the reservoir compensated for water loss from seepage and evaporation. Constricted tagelus, Sinonovacula con- stricta, was selected as the cultured mollusk, and Pacific white shrimp, Litopenaeus vannamei, as the cultured shrimp. The main managing measures during the production cycle were: setting and using the aerators; introduc- ing the probiotic products timely into the shrimp ponds; adopting a "pen-closing" method for controlling shrimp viral epidemics; setting the flow diversion barriers in the mollusk pond to keep the circulating water flowing through the pond along a sine-like curve and serve as substrate for biofilm; no direct feeding was necessary for the cultured mollusk until the co-cultured shrimp was harvested; natural foods in the water from the shrimp ponds was used for their foods. Two sets of the system were used in the experiment in 2002 and satisfactory re- sults were achieved. The average yield of the shrimp was 11 943.5 kg/hm2, and that of the mollusk was 16 965 kg/hm2. After converting the mollusk yield into shrimp yield at their market price ratio, the food coefficient of the entire system averaged at as low as 0.81. The water quality in the ponds was maintained at a desirable level and no viral epidemics were discovered during the production cycle.
基金Under the auspices of the Visvesvaraya National Institute of Technology(Nagpur)Centrally Funded Technical Institution Under the Ministry of Human Resource Development(No.l7-2/2014-TS.I)Department of Science and Technology,Government of India(No.SR/S9/Z-09/2012)
文摘Groundwater is one of the most important resources, its monitoring and optimized management has now become the priority to satisfy the demand of rapidly increasing population. In many developing countries, optimized groundwater level monitoring networks are rarely designed to build up a strong groundwater level data base, and to reduce operation time and cost. The paper presents application of geostatistical method to optimize existing network of observation wells for 18 sub-watersheds within the Wainganga Sub-basin located in the central part of India. The average groundwater level fluctuation(GWLF) from 37 observation wells is compared with parameters like lineament density, recharge, density of irrigation wells, land use and hydrogeology(LiRDLH) of Wainganga Sub-basin and analyzed stochastically in Geographic Information System(GIS) environment using simple, ordinary, disjunctive and universal kriging methods. Semivariogram analyses have been performed separately for all kriging methods to fit the best theoretical model with experimental model. Results from gaussian, spherical, exponential and circular theoretical models were compared with those of experimental models obtained from the groundwater level data. Spatial analyses conclude that the exponential semivariogram model obtained from ordinary kriging gives the best fit model. Study demonstrates that ordinary kriging gives the optimal solution and additional number of observation wells can be added utilizing the error variance for optimal design of groundwater level monitoring networks. This study describes the use of Geostatistics methods in GIS to predict the groundwater level and upgrade groundwater level monitoring networks from the randomly distributed observation wells considering multiple parameters such as GWLF and LiRDLH. The method proposed in the present study is observed to be an efficient method for selecting observation well locations in a complex geological set up. The study concludes that minimum 82 wells are required for proper monitoring of groundwater level in the study area.
基金Supported by the National Natural Science Foundation of China(Nos.31572255,41522604,31301867)the Strategic Priority Research Program of CAS(No.XDA11020702)the Science and Technology Development Program of Yantai(No.2014ZH073)
文摘Peritrich ciliates are highly diverse and can be important bacterial grazers in aquatic ecosystems. Morphological identifi cations of peritrich species and assemblages in the environment are time-consuming and expertise-demanding. In this study, two peritrich-specifi c PCR primers were newly designed to amplify a fragment including the internal transcribed spacer(ITS) region of ribosomal rDNA from environmental samples. The primers showed high specifi city in silico, and in tests with peritrich isolates and environmental DNA. Application of these primers in clone library construction and sequencing yielded exclusively sequences of peritrichs for water and sediment samples. We also found the ITS1, ITS2, ITS, D1 region of 28 S rDNA, and ITS+D1 region co-varied with, and generally more variable than, the V9 region of 18 S rDNA in peritrichs. The newly designed specifi c primers thus provide additional tools to study the molecular diversity, community composition, and phylogeography of these ecologically important protists in dif ferent systems.
文摘Miyun Reservoir was designated as the water source of Beijing City in 1982. Since that time, socio economic development in Miyun Area has been slowing due to the restriction of severe environmental standards. More and more attention from the public and government has been paid to the regional sustainable development. And an effective planning for the local society management system is urgently desired. In this study, a regional sustainable development system dynamics model, named MiyunSD, is developed for supporting this planning task. MiyunSD consists of dynamic simulation models that explicitly consider information feedback that governs interactions in the system. Such models are capable of simulating the system′s behavior and predicting its developing situation of the future. For the study case, interactions among a number of system components within a time frame of fifteen years are examined dynamically. Three planning alternatives are carefully considered. The base run is based on an assumption that the existing pattern of human activities will prevail in the entire planning horizon, and the other alternatives are based on previous and present planning studies. The different alternatives will get different system′s environmental and socio economic results. Through analyzing these dynamic results, local authorities may find an optimal way to realize the objectives that the regional environment will be well protected and at the same time the economy will be rapidly developed.
文摘Tunnels in an urban area, in many cases, are constructed in soft ground which contains underground water, near existing facilities and structures. Structural stability for the tunnel and also the nearby structures and facilities is vital in this kind of work. Slurry pipe jacking was firmly established as a special method for the non-disruptive construction of underground pipelines of sewage systems. This method utilizes mud slurry that is formed around the pipes in order to stabilize the surrounding soil. In the pipe roof method the tubing elements that are constructed by slurry pipe jacking are near each other longitudinally, and create a rigid and stable lining before the excavation of the main tunnel. This paper discusses 'the application of a slurry pipe jacking system on 'the pipe roof method by means of numerical analysis. Because of the rigid behavior of the lining, the results show little subsidence, making this method a reliable method of constructing large tunnels with small cover in an urban area.
基金Projects(ZR2013BL010,ZR2012DL05)supported by the Natural Science Foundation of Shandong Province,ChinaProject(4041412016)supported by the Research Excellence Award of Shandong University of Technology,ChinaProjects(2013GG03116,2011GG02115)supported by the Science and Technology Development Planning Project of Zibo,China
文摘High salinity industrial wastewater is difficult to treat using biological treatment system because of the high concentrations of salt.The potential of a sequencing batch biofilm reactor(SBBR)process in treating synthetic high salinity wastewater was evaluated at laboratory scale during a 110-day operation.The reactor was operated in a 12 h cycle,and each cycle consisted of 0.25 h influent addition,8 h aeration,3 h anoxic reaction,0.5 h sedimentation and 0.25 h effluent withdrawal.Gradual increase in salinity gradient was applied during the acclimatization period.The acclimated SBBR system was demonstrated to be an effective process to remove organic compounds and ammonia nitrogen under high salinity conditions with chemical oxygen demand(COD)and ammonia nitrogen(NH3-N)removal efficiencies of 88% and 80%,respectively.The microscopic examination indicated that rather than rotifers or vorticella,the zoogloea,filamentous fungus mingled with a small quantity of swimming infusorians were dominant bacteria in SBBR system.The removal efficiencies close to 80% in COD and 75% in NH3-N were achieved at an organic loading rate(OLR)of 0.96 kg COD/(m3·d),pH of 7.0,salinity of 14 g/L and NH3-N of 30 mg/L.