改良琼脂培养基稀释法在抗真菌药物体外敏感性试验中的应用研究

目的:初步观测改良琼脂培养基稀释法和微量液体培养基稀释法在测定白色念珠菌对伊曲康唑体外敏感性上的差异。方法:采用CLSI M27-A2推荐的微量液体培养基稀释法和改良琼脂培养基稀释法同时测定20株临床近期分离无重复白色念珠菌对伊曲康唑的体外敏感性。结果:两种方法对检测的20株真菌的体外最小抑菌浓度一致。结论:改良琼脂培养基稀释法有可能成为一种评价抗真菌药物体外敏感性的一种直观、简单、快速的方法。

胡萝卜花粉生活力测定方法筛选

采用染色法、琼脂培养基法、液体培养基法对胡萝卜花粉生活力进行测定,在对胡萝卜花粉形态进行初步了解的同时,筛选出较适合胡萝卜花粉生活力测定的方法。结果表明,本试验条件下,较适宜胡萝卜花粉萌发的方法为PEG+BK培养基离体培养法。

不同花型芍药的花粉生活力测定和比较研究

对芍药单瓣型、金环型、台阁型、皇冠型和菊花型等不同花型的10个品种,采用TTC法和琼脂培养基发芽法分别进行花粉生活力的测定和比较研究。显微观察结果表明,单瓣型品种的花粉形态和其他几种花型芍药的花粉有所不同。TTC染色法结果表明,单瓣型品种的花粉生活力与金环型、台阁型、皇冠型、菊花型品种间存在极显著差异,菊花型与台阁型、金环型间有显著差异,金环型、台阁型和皇冠型之间均无显著差异;单瓣型品种花粉生活力最强,菊花型的最弱。琼脂培养基发芽法结果表明,以蔗糖10%、琼脂0.5%、硼酸10 mg/L培养基为佳;花粉生活力测定值较TTC法低,数据更为准确。

17种梅(桃)属观赏树木花粉生活力的比较

用琼脂培养基发芽法,对梅(桃)属(Prunus)观赏树种中的梅(P.mume)、红梅(P.mume f.rubriflo ra)、杏(P.armeniace)、李(P.saliciua)、紫叶李(P.cerasifera var.atropupura)、郁李(P.japonica)、东京樱花(P.yedoensis)、毛樱桃(P.tomentosia)、榆叶梅(P. triloba)、桃(P.persica)、白花桃(P.persica f.alba)、蟠桃(P.persica f.compressa)、紫叶桃(P.persica f.atropurpurea)、红碧桃(P.persica f.rubro plena)、白碧桃(P.persica f.albo plena)、红寿星桃(P.persica f.rubro densa)、白寿星桃(P.persica f.albo densa)计 17 份种质,进行花粉生活力测定研究。统计结果表明:供试梅(桃)属观赏树种中,种(变种)间花粉生活力存在显著性差异,桃种若干栽培变型间也大都存在显著性差异。琼脂培养基 pH处理(5.4,5.8,6.2)间具显著性差异,且以pH 5.8为最佳;琼脂培养基蔗糖处理(质量分数10%,15%)间无显著性差异。

Assessment of Water Microbiologic Pollution in Durres＇s Marine Harbour Basin, Albania

The aim of this study is to assess the water microbiologic pollution in Durres＇s Harbour basin and to compare it with European standards. The comparison of heterotrophs and total coliforms level in sampling are the essence of this research. The object of this study is done in four sampling areas of Durres＇s Harbour basin. In order to compare the level of water microbiologic pollution in four areas of Durres＇s Harbour basin, control area is also studied which is a beach area near the Harbour named Apollonia Beach. The sampling areas were： Ferry Terminal （FT）, Fishery Harbor （FH）, East Zone （EZ）, Fuel Quay （FQ） and Apollonia Beach （AB）, respectively. The period of sample-taking was July-October 2008. The strategy used for this purpose consisted in water insemination with coverage method by means of Petri＇s plates according to respective dilutions in culture media Plate Count Agar （PCA） for heterotrophs and MacConkey for total coliforms. The number of colonies that are formed determines the number of cells at the moment of water insemination, the number of heterotrophs in culture media PCA, respectively. The number of pink and red colonies that were formed determines the number of cells at the moment of water insemination, the number of total coliforms in culture media MacConkey, respectively. The measure ofheterotrophic bacteria and total coliforms used is Colony-Forming Units （CFU）/100 mL seawater. （AB） is within European standards. The richest area with heterotrophs is （FH）, which confirms the fact that it is the most polluted microbiologic area in the Harbour basin of Durres. The richest area with total coliforms is （FT）. The poorest area with heterotrophs and total coliforms is （FQ）.