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RNA干扰对阿尔茨海默病家族性瑞典型APP突变的沉默作用邱
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作者 邱昕 潘纪安 +3 位作者 陈宇 张玮莹 杨华静 张苏明 《神经科学通报》 CSCD 2005年第3期219-223,共5页
目的诱导小干涉RNA (small interference RNA, siRNA )对阿尔茨海默病淀粉样前体蛋白家族性瑞典型突变(APPswe)的沉默作用。方法设计一对针对APPswe的寡核苷酸,构建成内源性表达相应siRNA的质粒,与构建的APP-EGFP重组质粒瞬时共转染COS-... 目的诱导小干涉RNA (small interference RNA, siRNA )对阿尔茨海默病淀粉样前体蛋白家族性瑞典型突变(APPswe)的沉默作用。方法设计一对针对APPswe的寡核苷酸,构建成内源性表达相应siRNA的质粒,与构建的APP-EGFP重组质粒瞬时共转染COS-7细胞,利用GFP报告基因来间接观察siRNA对APPswe的沉默作用。结果siRNA可有效地下调突变型APP,相比APPwt而言,针对突变型APP的siRNA对APPswe的沉默作用更强。结论针对APPswe的siRNA可有效地沉默APPswe基因,并有一定的等位基因特异性。因此,针对APPswe的siRNA将在阿尔茨海默病治疗研究领域扮演重要角色。 展开更多
关键词 RNA干扰 阿尔茨海默病 家族性 瑞典型 APP突变 沉默作用 SIRNA AD
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非瑞典型远端肌营养不良1例临床及病理报告
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作者 吴晶莹 樊东升 钟延丰 《神经疾病与精神卫生》 2002年第1期62-62,共1页
进行性肌营养不良为临床常见遗传性疾病,但多为近端起病。远端型肌营养不良临床甚为少见,国内迄今报道不多。现将我院1例非瑞典型患者资料报道如下。
关键词 肌营养不良症 瑞典型远端肌营养不良症 病理 病例报告
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携带淀粉样前体蛋白瑞典型突变基因转基因小鼠的制备及体内表达研究 被引量:1
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作者 刘薇 卢光琇 《中华医学遗传学杂志》 EI CAS CSCD 2002年第2期104-107,共4页
目的 建立携带人类淀粉样前体蛋白瑞典型突变 (Swedish mutation of amyloid precursorprotein,APPSWE)基因的转基因小鼠模型。方法 采用受精卵原核显微注射法 ,将人类 APPSWE转基因导入C5 7及昆明种小鼠受精卵内 ,然后将注射后保持... 目的 建立携带人类淀粉样前体蛋白瑞典型突变 (Swedish mutation of amyloid precursorprotein,APPSWE)基因的转基因小鼠模型。方法 采用受精卵原核显微注射法 ,将人类 APPSWE转基因导入C5 7及昆明种小鼠受精卵内 ,然后将注射后保持完整的受精卵移植到假孕母鼠的输卵管内 ,然后应用聚合酶链反应 (polymerase chain reaction,PCR)、荧光原位杂交及逆转录 PCR分析子代小鼠中外源基因的整合及表达情况。结果 注射后卵的存活率和幼子出生率分别为 76 .6 2 %和 10 .38% ;外源基因整合率为 35 .2 9% ;共获得 6只首建小鼠 ,已稳定传 3代 ,PCR检测共 5 5只阳性 ;提取阳性小鼠心、脑、肝、肾组织及骨骼肌以人类 APPSWE基因外显子特异的引物进行逆转录 PCR分析 ,结果发现其心、脑组织及骨骼肌中具有人类APPSWE基因的表达。结论 说明携带淀粉样前体蛋白瑞典型突变基因的转基因小鼠模型已制备成功。 展开更多
关键词 转基因动物模型 淀粉样前体蛋白瑞典型突变基因 阿尔茨海默病 基因突变
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阿尔茨海默病相关转基因小鼠模型的构建
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作者 刘薇 卢光琇 《生命科学研究》 CAS CSCD 2001年第4期329-332,共4页
将人类淀粉样前体蛋白瑞典型突变基因 (APPSW)与血小板衍生的生长因子 α链 (PDGFα)的顺式调控因子结合 ,构建成 PDGF- APPSW转基因 ;并通过原核显微注射法将之导入 C5 7小鼠受精卵内 ,成功地获得PDAPSW转基因小鼠模型 ,共出生 2 2只仔... 将人类淀粉样前体蛋白瑞典型突变基因 (APPSW)与血小板衍生的生长因子 α链 (PDGFα)的顺式调控因子结合 ,构建成 PDGF- APPSW转基因 ;并通过原核显微注射法将之导入 C5 7小鼠受精卵内 ,成功地获得PDAPSW转基因小鼠模型 ,共出生 2 2只仔鼠 ,其中 5只基因组内有 展开更多
关键词 淀粉样前体蛋白基因 瑞典型突变 转基因小鼠模型 阿尔茨海默病 AD
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Framework for assessing quality of care for inflammatory bowel disease in Sweden 被引量:2
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作者 Martin Rejler Jrgen Tholstrup +2 位作者 Mattias Elg Anna Spngéus Boel Andersson Gre 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第10期1085-1092,共8页
AIM: To create and apply a framework for quality assessment and improvement in care for inflammatory bowel disease (IBD) patients. METHODS A framework for quality assessment and improvement was created for IBD based o... AIM: To create and apply a framework for quality assessment and improvement in care for inflammatory bowel disease (IBD) patients. METHODS A framework for quality assessment and improvement was created for IBD based on two generally acknowledged quality models. The model of Donabedian (Df) offers a logistical and productive perspective and the Clinical Value Compass (CVC) model adds a management and service perspective. The framework creates a pedagogical tool to understand the balance between the dimensions of clinical care (CVC) and the components of clinical outcome (Df). The merged models create a framework of the care process dimensions as a whole, reflecting important parts of the IBD care delivery system in a local setting. Clinical and organizational quality measures were adopted from clinical experience and the literature and were integrated into the framework. Data were collected at the yearly check-up for 481 IBD patients during 2008. The application of the quality assessment framework was tested and evaluated in a local clinical IBD care setting in Jnkping County, Sweden. RESULTS: The main outcome was the presentation of how locally-selected clinical quality measures, integrated into two complementary models to develop a frame-work, could be instrumental in assessing the quality of care delivered to patients with IBD. The selected quality measures of the framework noted less anemia in the population than previously reported, provided information about hospitalization rates and the few surgical procedures reported, and noted good access to the clinic. CONCLUSION: The applied local quality framework was feasible and useful for assessing the quality of care delivered to IBD patients in a local setting. 展开更多
关键词 Quality measures Inflammatory bowel disease Value compass Donabedian Quality improvement
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Female Entrepreneurship in Switzerland: Specificities and Differences
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作者 Mathias Rossi Silna Borter, Marie Sansonnens 《Journal of Modern Accounting and Auditing》 2012年第8期1225-1236,共12页
Women entrepreneurs are increasingly representing an underexploited potential of the Swiss economy. Although entrepreneurship represents a rapidly expanding phenomenon, women meet specific difficulties that explain th... Women entrepreneurs are increasingly representing an underexploited potential of the Swiss economy. Although entrepreneurship represents a rapidly expanding phenomenon, women meet specific difficulties that explain this lack of exploitation by the economy, The Global Entrepreneurship Monitor (GEM), a not-for-profit academic research consortium, published its annual report which mentioned in particular a difference between female and male entrepreneurship. According to these results, it can be noticed that the typical profile of the female entrepreneur differs from that of her male counterpart. In Switzerland, those differences can be situated at three levels: (I) In the first place, a natural difference of the female entrepreneurship at the level of activities (service activities, low-added value, and low potential of job creation); (2) Secondly, a difference in motivation/commitment (part-time work, start-up funds available, skills, received opportunities, and fear of failure); and (3) Thirdly, a difference at the level of insertion (revealing the importance of networking, access to financing and structures of encouragement). The present communication points up a typical profile of the woman entrepreneur in Switzerland. The authors also intend to identify the kind of obstacles female entrepreneur is likely to meet as well as the existing initiatives to exceed stereotypes by taking into account her specificities. There are several supports and measures intended to help women entrepreneurship concerning the following aspects: better time management; adapted following and training structures; access to easier financing; and better visibility and networking. 展开更多
关键词 ENTREPRENEURSHIP female entrepreneurship Swiss entrepreneurship GENDER gender differences
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A multi-state weather generator for daily precipitation for the Torne River basin, northern Sweden/western Finland
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作者 David RAYNER Christine ACHBERGER Deliang CHEN 《Advances in Climate Change Research》 SCIE CSCD 2016年第1期70-81,共12页
This paper describes a new weather generator e the 10-state empirical model e that combines a 10-state, first-order Markov chain with a non-parametric precipitation amounts model. Using a doubly-stochastic transition-... This paper describes a new weather generator e the 10-state empirical model e that combines a 10-state, first-order Markov chain with a non-parametric precipitation amounts model. Using a doubly-stochastic transition-matrix results in a weather generator for which the overall precipitation distribution(including both wet and dry days) and the temporal-correlation can be modified independently for climate change studies. This paper assesses the ability of the 10-state empirical model to simulate daily area-average precipitation in the Torne River catchment in northern Sweden/western Finland in the context of 3 other models: a 10-state model with a parametric(Gamma) amounts model; a wet/dry chain with the empirical amounts model; and a wet/dry chain with the parametric amounts model. The ability to accurately simulate the distribution of multi-day precipitation in the catchment is the primary consideration.Results showed that the 10-state empirical model represented accumulated 2- to 14-day precipitation most realistically. Further, the distribution of precipitation on wet days in the catchment is related to the placement of a wet day within a wet-spell, and the 10-state models represented this realistically, while the wet/dry models did not. Although all four models accurately reproduced the annual and monthly averages in the training data, all models underestimated inter-annual and inter-seasonal variance. Even so, the 10-state empirical model performed best.We conclude that the multi-state model is a promising candidate for hydrological applications, as it simulates multi-day precipitation well, but that further development is required to improve the simulation of interannual variation. 展开更多
关键词 Weather generator MULTI-STATE Torne River PRECIPITATION
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阿尔茨海默病相关PDAP_(SW)转基因小鼠模型的表型研究 被引量:2
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作者 刘薇 卢光秀 《中华神经科杂志》 CAS CSCD 北大核心 2002年第4期201-203,共3页
目的 探讨淀粉样前体蛋白 (APP)基因瑞典型突变 (APPSW)在Alzheimer病 (AD)发病中的作用。方法 对PDAPSW转基因小鼠的毛发、体重及生殖能力进行观察 ;采用免疫组化方法对转基因小鼠大脑组织进行病理改变分析 ;采用国际通用的Y迷宫分... 目的 探讨淀粉样前体蛋白 (APP)基因瑞典型突变 (APPSW)在Alzheimer病 (AD)发病中的作用。方法 对PDAPSW转基因小鼠的毛发、体重及生殖能力进行观察 ;采用免疫组化方法对转基因小鼠大脑组织进行病理改变分析 ;采用国际通用的Y迷宫分析转基因小鼠的行为学异常。结果 3个月龄时首建鼠平均体重为 (31.0± 3 .7)g ,非转基因小鼠为 (34.0± 2 .9)g ,F1代转基因小鼠平均体重为 (32 .0± 3 .3)g ,阴性同胞鼠为 (31.0± 4.2 )g ,两组体重差异无显著意义 ;两组转基因小鼠毛发及生殖能力差异均无显著意义 ,但发现其中 3只阳性小鼠毛发异常 ,1只雄性小鼠不育 ;转基因小鼠脑组织免疫组化分析发现明显淀粉样蛋白沉积 ;Y迷宫内转基因小鼠进入迷宫各臂均数为 (5 3± 7)次 ,非转基因鼠为 (37± 4)次 ;轮回转向频率分别为 48.2 %和 76 .4% ,两组间差异有显著意义。结论 PDAPSW转基因小鼠脑组织内有淀粉样蛋白沉积并且存在行为学异常 ,能够复制人类AD的主要变化 ,可以作为深入研究AD发病机制的动物模型。 展开更多
关键词 阿尔茨海默病 PDAPSW转基因小鼠 淀粉样Β蛋白前体 免疫组织化学 动物模型 基因瑞典型突变
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人参皂苷Re对瞬时转染APPswe N2a细胞内β2-肾上腺受体及β-样淀粉蛋白肽的影响 被引量:2
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作者 姜霞 刘萍 +1 位作者 李凡 王平 《中华中医药杂志》 CAS CSCD 北大核心 2019年第6期2397-2401,共5页
目的:研究人参皂苷Re对瞬时转染瑞典型突变淀粉样前体蛋白(APPswe)cDNA的小鼠神经瘤母细胞(N2a细胞)内β2-肾上腺受体(β2-AR)及β-样淀粉蛋白肽(Aβ)的影响。方法:瞬时转染APPswe质粒入N2a细胞24h后,加入1、10及100μmol/L浓度的人参皂... 目的:研究人参皂苷Re对瞬时转染瑞典型突变淀粉样前体蛋白(APPswe)cDNA的小鼠神经瘤母细胞(N2a细胞)内β2-肾上腺受体(β2-AR)及β-样淀粉蛋白肽(Aβ)的影响。方法:瞬时转染APPswe质粒入N2a细胞24h后,加入1、10及100μmol/L浓度的人参皂苷Re处理细胞24h后收集细胞。应用点印迹检测4G8(识别Aβ17-24片段)的表达,免疫印迹检测APP、β2-AR、β2-AR丝氨酸346位点磷酸化(pS346-β2-AR)和β2-AR苏氨酸68位点磷酸化(pT68-β2-AR)的表达,并采用免疫细胞化学技术分析统计pS346-β2-ARβ及β2-AR的染色强度。结果:瞬时转染APPswe质粒的N2a细胞内pS346-β2-AR的表达较pcDNA组增强(P<0.05),pT68-β2-AR的表达较pcDNA组减弱(P<0.05)。与APPswe组比较,1、10、100μmol/L的人参皂苷Re可降低pS346-β2-AR的表达(P<0.01),人参皂苷Re不能逆转pT68-β2-AR的表达水平。免疫细胞化学统计显示,转染APPswe质粒的N2a细胞内pS346-β2-AR染色强度较对照组显著增强(P<0.05),人参皂苷Re可减弱其表达。β2-AR的表达在各组无显著差异。转染APPswe质粒的N2a细胞内APP及4G8的表达较对照组显著增加(P<0.01);与APPswe组比较,比较,100μmol/L的人参皂苷Re可减少APP蛋白的表达(P<0.05),10μmol/L及100μmol/L的人参皂苷Re降低4G8表达(P<0.05)。结论:人参皂苷Re可降低瞬时转染APPswe质粒的N2a细胞内pS346-β2-AR的表达水平,并显著减少该细胞内APP及Aβ的表达,改善AD的基本病变。 展开更多
关键词 人参皂苷RE N2A细胞 瑞典型突变淀粉样前体蛋白 β-样淀粉蛋白肽 β2-肾上腺受体
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