This study aimed to investigate the influence of Glycyl-glutamine on the development of immune functions of Yuehuang chicken, and to investigate its effect on proliferation and secretion of chicken lymphocytes. In exp...This study aimed to investigate the influence of Glycyl-glutamine on the development of immune functions of Yuehuang chicken, and to investigate its effect on proliferation and secretion of chicken lymphocytes. In experiment one ,360 1-day-old Yuehuang chicken were randomly divided into 4 groups, with 90 birds in each group. Group 1 served as control, while group 2 to 4 were respectively supplemented ad libitum with 100,500 and 1 000 μg/L Gly-Gln via drinking water. On 45 and 80 days of age, 18 birds in each group were slaughtered and the bursa of Fabricius , spleen and thymus were weighted and blood samples were collected. At the same times, 3 birds in each group were selected and lymphocytes from blood and spleen were collected for measuring proliferation and secretion of IL-2 and IL-6. Experiment two was conducted to test the proliferation and secretion of lymphocytes when cultured with the addition of Gly-Gln. Lymphocytes from blood and spleen were isolated from 8-week-old chicken without treatment of Gly-Gln, and culture in media added with 0, 0.6,1.2 and 2.4 mmol/L Glycyl-glutamine solution respertively. The results showed : (1) Supplementation of Gly-Gln had no significant effect on the development of Fabricius Bursa; but Gly-Gln significantly decreased the thymus indices at 1 000 μg/L level and improved the spleen indices at 500 μg/L level in 80-day-old chickens. (2) The additions of both 500 μg/L and 1 000 (μg/L ) Gly-Gln significantly increased concentrations of IL-2 and IL-6 in the sera of Yuehuang chicken on 45 and 80 days of age, and enhanced blood lymphocyte proliferation , but inhibited spleen lymphocyte proliferation in 45-day-old chicken.(3) The addition of Gly-Gln at concentration of 2.4 mmol/L in the culture medium significantly enhanced lymphocyte proliferation ,but Gly-Gln did not affect the secretion of IL-2 and IL-6 form cultured lymphocytes.展开更多
文摘This study aimed to investigate the influence of Glycyl-glutamine on the development of immune functions of Yuehuang chicken, and to investigate its effect on proliferation and secretion of chicken lymphocytes. In experiment one ,360 1-day-old Yuehuang chicken were randomly divided into 4 groups, with 90 birds in each group. Group 1 served as control, while group 2 to 4 were respectively supplemented ad libitum with 100,500 and 1 000 μg/L Gly-Gln via drinking water. On 45 and 80 days of age, 18 birds in each group were slaughtered and the bursa of Fabricius , spleen and thymus were weighted and blood samples were collected. At the same times, 3 birds in each group were selected and lymphocytes from blood and spleen were collected for measuring proliferation and secretion of IL-2 and IL-6. Experiment two was conducted to test the proliferation and secretion of lymphocytes when cultured with the addition of Gly-Gln. Lymphocytes from blood and spleen were isolated from 8-week-old chicken without treatment of Gly-Gln, and culture in media added with 0, 0.6,1.2 and 2.4 mmol/L Glycyl-glutamine solution respertively. The results showed : (1) Supplementation of Gly-Gln had no significant effect on the development of Fabricius Bursa; but Gly-Gln significantly decreased the thymus indices at 1 000 μg/L level and improved the spleen indices at 500 μg/L level in 80-day-old chickens. (2) The additions of both 500 μg/L and 1 000 (μg/L ) Gly-Gln significantly increased concentrations of IL-2 and IL-6 in the sera of Yuehuang chicken on 45 and 80 days of age, and enhanced blood lymphocyte proliferation , but inhibited spleen lymphocyte proliferation in 45-day-old chicken.(3) The addition of Gly-Gln at concentration of 2.4 mmol/L in the culture medium significantly enhanced lymphocyte proliferation ,but Gly-Gln did not affect the secretion of IL-2 and IL-6 form cultured lymphocytes.
