Continuing the previous report(1),seventeen compounds were isolated from the roots and rhizoma of Glycyrrhiza inflata Bat. On the basis of spectroscopic methods five of them were identified as sucrose(1),4’,5,7-trihy...Continuing the previous report(1),seventeen compounds were isolated from the roots and rhizoma of Glycyrrhiza inflata Bat. On the basis of spectroscopic methods five of them were identified as sucrose(1),4’,5,7-trihydroxy-8-prenyl-flavonc(2),liquiritigenin(3),formononetin(4),and glabrone(5)(1),(4)and(5)were obtained from this plant for the first time. Their antioxidant activities were observed in three oxidizing systems in vitro.(2),(3)and(5)showed remarkable activities in scavenging(5 exhibited good activity in inhibiting hemolysis induced by H_2O_2(3)and(5)displayed higher inhibitory action on (HpD + hv)-induced hemolysis than som typical antioxidants.展开更多
Prenylated flavonoids are mainly distributed in Leguminosae and Moraceae plants, and they have been reported to possess various biological activities. Previously, we have reported a prenylated isoflavonoid, isoangusto...Prenylated flavonoids are mainly distributed in Leguminosae and Moraceae plants, and they have been reported to possess various biological activities. Previously, we have reported a prenylated isoflavonoid, isoangustone A（IAA） from licorice（Glycyrrhiza uralensis）, which induces apoptosis in colorectal cancer cells by disrupting mitochondrial functions. In the present study, we compared a group of flavonoids from licorice with IAA for their anti-proliferation activities and effects on intracellular signaling. The results indicated that the isoprenyl groups on the A and B rings, the hydroxyl groups at the ortho position of isoprenyl on A ring and the conjugated plane of C ring might contribute to the anti-cancer activity of prenylated flavonoids. Based on the above structure-activity relationship, we further identified four prenylated flavonoids with similar anti-cancer activities from licorice. Taken together, our present study established a preliminary structure-activity relationship of anti-cancer prenylated flavonoids, and our data provided important leading compounds from licorice, which deserved further research and development.展开更多
To determine 13 flavonoids and glycyrrhizic acid in licorice （Glycyrrhiza spp.）, several samples from different areas were examined by HPLC-DAD analysisThe analysis was performed on a Zorbax Extend-C18 （250 mm×...To determine 13 flavonoids and glycyrrhizic acid in licorice （Glycyrrhiza spp.）, several samples from different areas were examined by HPLC-DAD analysisThe analysis was performed on a Zorbax Extend-C18 （250 mm× 4.6 mm, 5 μm） column connected with a Zorbax Extend guard column （20 mm × 4.6 mm, 5 μm）. The mobile phase consisted of （A） acetonitrile and （B） 0.026% aqueous H3PO4 （V/V） using a gradient elution of 20%-25% A at 0-20 min, 25%-33% A at 20-30 min, 33%-50% A at 30-55 min, 50%-60% A at 55-65 min, and 60% A between 65 min and 80 min, and peaks were detected at 280 rim. The fourteen compounds were assigned by HPLC-Orbitrap MS methods. The regression coefficient for the linear equations for the 14 compounds ranged between 0.9998 and 1. The limits of detection and quantification lay in the range of 0.032-2.461 and 0.154-8.202 μg·mL^-1, respectively. The relative recovery rates for the 14 compounds were in the range of 93.90%-106.73% with RSDs being less than 5%. Coefficient variations for intra-day and inter-day precisions were in the range of 0.27%-2.38% and 0.31%-3.51%, respectively. In summary, the validated method was applied to the simultaneous determination of the 14 components in 29 different licorice samples and was proven to be suitable for quality evaluation of licorices and their active fractions.展开更多
In the present study, we aimed to perform a phytochemical investigation of the whole herb of Matricaria chamomilla L., a Uygur herbal medicine. A total of 18 phenolic compounds were isolated by silica gel and Sephadex...In the present study, we aimed to perform a phytochemical investigation of the whole herb of Matricaria chamomilla L., a Uygur herbal medicine. A total of 18 phenolic compounds were isolated by silica gel and Sephadex LH-20 chromatography, together with preparative HPLC methods. By analysis of the MS and NMR spectroscopic data and comparison with those in literature, these 18 compounds were identified as apigenin （1）, galangin （2）, luteolin （3）, kempherol （4）, quercetin （5）, hispidulin （6）, 6-methoxykaempferol （7）, eupafolin （8）, 3-methylquercetin （9）, ermanin （10）, 5,7,4＇-trihydroxy-3,6-dimethoxyflavonone （11）, bracteoside （12）, 7-O-（β-D-glucopyranosyl）-galactin （13）, isochlorogenic acid B （14）, isochlorogenic acid C （15）, 4-hydroxybenzoic acid （16）, 5-pentadecylbenzene-l,3-diol （17） and scopoletin （18）. Among them, compounds 1-13 were identified as flavonoids, Compounds 2 and 6-17 were isolated from both the nlant and the Matricaria genus for the first time.展开更多
文摘Continuing the previous report(1),seventeen compounds were isolated from the roots and rhizoma of Glycyrrhiza inflata Bat. On the basis of spectroscopic methods five of them were identified as sucrose(1),4’,5,7-trihydroxy-8-prenyl-flavonc(2),liquiritigenin(3),formononetin(4),and glabrone(5)(1),(4)and(5)were obtained from this plant for the first time. Their antioxidant activities were observed in three oxidizing systems in vitro.(2),(3)and(5)showed remarkable activities in scavenging(5 exhibited good activity in inhibiting hemolysis induced by H_2O_2(3)and(5)displayed higher inhibitory action on (HpD + hv)-induced hemolysis than som typical antioxidants.
基金National Natural Science Foundation of China(Grant No.81472657 and 81272468)
文摘Prenylated flavonoids are mainly distributed in Leguminosae and Moraceae plants, and they have been reported to possess various biological activities. Previously, we have reported a prenylated isoflavonoid, isoangustone A（IAA） from licorice（Glycyrrhiza uralensis）, which induces apoptosis in colorectal cancer cells by disrupting mitochondrial functions. In the present study, we compared a group of flavonoids from licorice with IAA for their anti-proliferation activities and effects on intracellular signaling. The results indicated that the isoprenyl groups on the A and B rings, the hydroxyl groups at the ortho position of isoprenyl on A ring and the conjugated plane of C ring might contribute to the anti-cancer activity of prenylated flavonoids. Based on the above structure-activity relationship, we further identified four prenylated flavonoids with similar anti-cancer activities from licorice. Taken together, our present study established a preliminary structure-activity relationship of anti-cancer prenylated flavonoids, and our data provided important leading compounds from licorice, which deserved further research and development.
基金supported by the National Natural Science Foundation of China(No.81001630)Natural Science Foundation of Shanghai(No.10ZR1436400)+1 种基金Major Projects of Knowledge Innovation Program of the Chinese Academy of Sciences(No.KSCX2-YW-R-166)the Twelfth Five-Year National Science&Technology Support Program(No.2012BAI29B06)
文摘To determine 13 flavonoids and glycyrrhizic acid in licorice （Glycyrrhiza spp.）, several samples from different areas were examined by HPLC-DAD analysisThe analysis was performed on a Zorbax Extend-C18 （250 mm× 4.6 mm, 5 μm） column connected with a Zorbax Extend guard column （20 mm × 4.6 mm, 5 μm）. The mobile phase consisted of （A） acetonitrile and （B） 0.026% aqueous H3PO4 （V/V） using a gradient elution of 20%-25% A at 0-20 min, 25%-33% A at 20-30 min, 33%-50% A at 30-55 min, 50%-60% A at 55-65 min, and 60% A between 65 min and 80 min, and peaks were detected at 280 rim. The fourteen compounds were assigned by HPLC-Orbitrap MS methods. The regression coefficient for the linear equations for the 14 compounds ranged between 0.9998 and 1. The limits of detection and quantification lay in the range of 0.032-2.461 and 0.154-8.202 μg·mL^-1, respectively. The relative recovery rates for the 14 compounds were in the range of 93.90%-106.73% with RSDs being less than 5%. Coefficient variations for intra-day and inter-day precisions were in the range of 0.27%-2.38% and 0.31%-3.51%, respectively. In summary, the validated method was applied to the simultaneous determination of the 14 components in 29 different licorice samples and was proven to be suitable for quality evaluation of licorices and their active fractions.
文摘In the present study, we aimed to perform a phytochemical investigation of the whole herb of Matricaria chamomilla L., a Uygur herbal medicine. A total of 18 phenolic compounds were isolated by silica gel and Sephadex LH-20 chromatography, together with preparative HPLC methods. By analysis of the MS and NMR spectroscopic data and comparison with those in literature, these 18 compounds were identified as apigenin （1）, galangin （2）, luteolin （3）, kempherol （4）, quercetin （5）, hispidulin （6）, 6-methoxykaempferol （7）, eupafolin （8）, 3-methylquercetin （9）, ermanin （10）, 5,7,4＇-trihydroxy-3,6-dimethoxyflavonone （11）, bracteoside （12）, 7-O-（β-D-glucopyranosyl）-galactin （13）, isochlorogenic acid B （14）, isochlorogenic acid C （15）, 4-hydroxybenzoic acid （16）, 5-pentadecylbenzene-l,3-diol （17） and scopoletin （18）. Among them, compounds 1-13 were identified as flavonoids, Compounds 2 and 6-17 were isolated from both the nlant and the Matricaria genus for the first time.
