[ Objective] The aim was to construct drought and saline-alkaline resistance plant expression vector with mannose as selective agent, and further breed unmarked resilient varieties. [ Method] The plant expression vect...[ Objective] The aim was to construct drought and saline-alkaline resistance plant expression vector with mannose as selective agent, and further breed unmarked resilient varieties. [ Method] The plant expression vector was constructed by using Chimonanthus praecox（ L. ）Link aquapor.in CpTIP cDNA and Escherichia coli pmi gene, combined stress resistance gene with mannose positive selection system. [ Result] The test successfully constructed the plant expression vector pPMI：：CpTIP. [ Conclusion] The constructed vector linked advantages of stress resistance gene and mannose positive selection system.展开更多
To check the applicabilities of the simple density equation and viscosity equation in the semi-ideal solution theory to nonelectrolyte solutions, the densities and viscosities were measured for the quaternary system m...To check the applicabilities of the simple density equation and viscosity equation in the semi-ideal solution theory to nonelectrolyte solutions, the densities and viscosities were measured for the quaternary system mannitol-sorbitol-D-glucose-HzO and its ternary subsystems mannitol-D-glucose-H2O and sorbitol-D-glucose-H2O at 298.15K. The results were used to test the applicability of the simple equations for the density and viscosity of the multicomponent nonelectrolyte solution. The agreements between the predicted and measured results are good.展开更多
Abstract： A simple optimized microplate-based method to assay endo-1,4-β-mannosidase activity was described as an improved high-throughput screening method. A series of experimental conditions were optimized. It is ...Abstract： A simple optimized microplate-based method to assay endo-1,4-β-mannosidase activity was described as an improved high-throughput screening method. A series of experimental conditions were optimized. It is revealed that the optimum measurement procedure is as follows： adding 50μL of diluted enzyme sample and 50 μL substrate, incubating at 45 ℃ for exactly 5 min in micro-plate, mixing with 100 μL 3,5-dinitrosalicylic acid （DNS） reagent, maintaining at boiling point for 15 rain, cooling down to room temperature before determining the ABS value at 540 nm using an ELISA micro-plate reader. The reaction volume of the optimized microplate-assay is reduced to 200μL from 2 500 μL used in the standard β-mannanase macro-assay. The optimized micro-assay is significantly more sensitive in all of the 643 candidates during endo-1,4-β-mannosidase screening. Statistical analyses show that the sensitivity of the optimized micro-method is significantly greater than that of the macro-assay. The optimized method is convenient, fast, and cheap for high throughput enzyme screening.展开更多
A short and efficient stereoselective synthesis of a styryllactone (-)-isoaltholactone has been achieved in seven steps and 33% overall yield, starting from the readily available carbohydrate D-mannose. The key steps ...A short and efficient stereoselective synthesis of a styryllactone (-)-isoaltholactone has been achieved in seven steps and 33% overall yield, starting from the readily available carbohydrate D-mannose. The key steps of our synthesis involve intramolecular tetrahydrofuran cyclization and one-pot acetonide deprotection-lactonization.展开更多
基金Supported by Sub-project of Special Fund in Ministry of Agriculture of Transgenic Plants " Cultivation of New Varieties of Anti-adversity Transgenic Soybeans"(2008ZX08004-2)~~
文摘[ Objective] The aim was to construct drought and saline-alkaline resistance plant expression vector with mannose as selective agent, and further breed unmarked resilient varieties. [ Method] The plant expression vector was constructed by using Chimonanthus praecox（ L. ）Link aquapor.in CpTIP cDNA and Escherichia coli pmi gene, combined stress resistance gene with mannose positive selection system. [ Result] The test successfully constructed the plant expression vector pPMI：：CpTIP. [ Conclusion] The constructed vector linked advantages of stress resistance gene and mannose positive selection system.
基金Supported by the National Natural Science Foundation of China （No.20276037）
文摘To check the applicabilities of the simple density equation and viscosity equation in the semi-ideal solution theory to nonelectrolyte solutions, the densities and viscosities were measured for the quaternary system mannitol-sorbitol-D-glucose-HzO and its ternary subsystems mannitol-D-glucose-H2O and sorbitol-D-glucose-H2O at 298.15K. The results were used to test the applicability of the simple equations for the density and viscosity of the multicomponent nonelectrolyte solution. The agreements between the predicted and measured results are good.
基金Project(31000350)supported by the National Natural Science Foundation of China
文摘Abstract： A simple optimized microplate-based method to assay endo-1,4-β-mannosidase activity was described as an improved high-throughput screening method. A series of experimental conditions were optimized. It is revealed that the optimum measurement procedure is as follows： adding 50μL of diluted enzyme sample and 50 μL substrate, incubating at 45 ℃ for exactly 5 min in micro-plate, mixing with 100 μL 3,5-dinitrosalicylic acid （DNS） reagent, maintaining at boiling point for 15 rain, cooling down to room temperature before determining the ABS value at 540 nm using an ELISA micro-plate reader. The reaction volume of the optimized microplate-assay is reduced to 200μL from 2 500 μL used in the standard β-mannanase macro-assay. The optimized micro-assay is significantly more sensitive in all of the 643 candidates during endo-1,4-β-mannosidase screening. Statistical analyses show that the sensitivity of the optimized micro-method is significantly greater than that of the macro-assay. The optimized method is convenient, fast, and cheap for high throughput enzyme screening.
基金supported by the MOST of China (2012ZX09502001-001)the National Natural Science Foundation of China (2012CB822101,21072217 and 21202193)
文摘A short and efficient stereoselective synthesis of a styryllactone (-)-isoaltholactone has been achieved in seven steps and 33% overall yield, starting from the readily available carbohydrate D-mannose. The key steps of our synthesis involve intramolecular tetrahydrofuran cyclization and one-pot acetonide deprotection-lactonization.
