葡甘露聚糖抑菌护理凝胶(Glucomannan antibacteria care gel,GACG)是本课题组开发的一款已上市的治疗女性阴道炎症的抑菌护理剂。本文介绍了GACG的制备工艺,通过建立高危型HPV 16假病毒感染人胚肾293FT细胞模型,重点研究GACG的主要活...葡甘露聚糖抑菌护理凝胶(Glucomannan antibacteria care gel,GACG)是本课题组开发的一款已上市的治疗女性阴道炎症的抑菌护理剂。本文介绍了GACG的制备工艺,通过建立高危型HPV 16假病毒感染人胚肾293FT细胞模型,重点研究GACG的主要活性成分白芨葡甘露聚糖、积雪草总苷和蛇床子精油对高危型人乳头瘤病毒16(Human papillomavirus type 16,HPV 16)的抗病毒作用。研究发现积雪草总苷和蛇床子精油对高危型HPV 16假病毒具有明显的抗病毒活性,分子对接进一步探究了积雪草苷、羟基积雪草苷以及蛇床子素对HPV 16抑制的可能机制。展开更多
Lectins play a crucial role in the innate immunity of invertebrates and vertebrates by recognizing and disposing of pathogens. We obtained the complete cDNA of a C-type lectin （EALecl） from Epinephelus akaara using ...Lectins play a crucial role in the innate immunity of invertebrates and vertebrates by recognizing and disposing of pathogens. We obtained the complete cDNA of a C-type lectin （EALecl） from Epinephelus akaara using RACE. The complete EALecl cDNA sequence was 827 bp. The 5-UTR and 3-UTR were 28 bp and 151 bp, respectively, in length. The sequence also contained a polyadenylation signal AATAAA and a poly（A） tail. The EALecl cDNA encodes polypeptides with 215 amino acids, including a signal peptide of 31 amino acids. The protein has a cysteine-rich region at the N terminal, a collagenous region characterized by G-X-Y repeats, a neck region, and a typical carbohydrate-recognition domain （CRD）, indicating that EALecl is a collectin. The key recognition positions of this CRD are EPD, isolated for the first time in fish. These are likely the interim types, between mannan-binding lectin and galactose-binding lectin. We evaluated the expression pattern of EALecl in 12 different tissues using RT-PCR. EALecl was expressed in all tissues, though at different levels. In addition, we inserted EALecl into an expression vector （pET-28a） for transformation into the BL21 engineering bacteria. Based on enzyme digestion and sequencing of the positive clone, we successfully constructed the EALecl recombinant expression vector.展开更多
基金Supported by the Natural Science Foundation of Fujian Province of China (No 2060203)New Century Excellent Talents supporting funding of Fujian Province
文摘Lectins play a crucial role in the innate immunity of invertebrates and vertebrates by recognizing and disposing of pathogens. We obtained the complete cDNA of a C-type lectin （EALecl） from Epinephelus akaara using RACE. The complete EALecl cDNA sequence was 827 bp. The 5-UTR and 3-UTR were 28 bp and 151 bp, respectively, in length. The sequence also contained a polyadenylation signal AATAAA and a poly（A） tail. The EALecl cDNA encodes polypeptides with 215 amino acids, including a signal peptide of 31 amino acids. The protein has a cysteine-rich region at the N terminal, a collagenous region characterized by G-X-Y repeats, a neck region, and a typical carbohydrate-recognition domain （CRD）, indicating that EALecl is a collectin. The key recognition positions of this CRD are EPD, isolated for the first time in fish. These are likely the interim types, between mannan-binding lectin and galactose-binding lectin. We evaluated the expression pattern of EALecl in 12 different tissues using RT-PCR. EALecl was expressed in all tissues, though at different levels. In addition, we inserted EALecl into an expression vector （pET-28a） for transformation into the BL21 engineering bacteria. Based on enzyme digestion and sequencing of the positive clone, we successfully constructed the EALecl recombinant expression vector.
