自主管理的“生产岛”

现代企业管理要求企业内的每一位员工都能发挥出最大能量。然而企业传统的管理机制限制了员工能量的发挥，因为传统的管理模式部门与部门职责交叉，存在责任模糊、部门间相互推诿扯皮的问题。按传统的管理模式，生产、维修、质保、工艺等部门各司其职，在生产过程中，机器故障报设备维修部门修理；产品质量由质保部门检查认可；工艺流程凭工艺部门制定实施，造成了产品制造者不管质量，管质量的不生产；操作设备的不管设备，管设备的不操作设备，使得生产与维修、质保、工艺等职责脱节，不仅造成效益上的巨大损失，同时又使得作为生产实施主体的员工完全处于被动的地位，限制了员工能量的发挥和提升自身价值的积极性。

铝合金铸件低压铸造智能生产岛的设计

以某项目为基础,生产商用车飞轮壳、新能源电机壳铸件,采用铝合金低压铸造工艺,以智能化生产实施为基本出发点,对各设备、各工序流程、各工序之间的衔接均设计数字化系统,完成整个智能生产岛的设计,为实现车间智能制造提供基础设备层条件。

基于生产组织方式变革的职业教育课程模式研究

职业教育借助课程模式为企业培养人才,课程模式的选择必然受企业生产组织方式制约。西方发达国家在不同发展时期主导的生产组织方式不同,职教课程模式也呈现出差异。古代以手工业为基础的工艺生产方式下,学徒制是课程模式的主要存在形态;福特主义和新福特主义经济下,生产线组织方式占主导,学科课程、技能训练课程、任务本位课程是职业教育在课程模式上所进行的探索与实践;后福特主义经济下,生产岛组织方式占主导,项目课程成为职教课程模式的最佳选择,也是现代职教课程改革的基本走向。

丰田公司安灯系统的内部管理启示

过去,很多工业企业提倡学习日本的丰田汽车。这种被美国人称为“精良生产”的经营管理模式,大大提高了生产效率和产品质量,降低了产品成本,不但促进了丰田汽车公司的发展,甚至影响到全日本和全世界。笔者深入剖析丰田公司独创的“安灯系统”,以独特视角揭示其带来的公司管理启示。

Enhancement of insulin-producing cell differentiation from embryonic stem cells using pax4-nucleofection method

AIM： To enhance the differentiation of insulin producing cell （IPC） ability from embryonic stem （ES） cells in vitro. METHODS： Four-day embryoid body （EB）-formatted ES cells were dissociated as single cells for the followed plasmid DNA delivery. The use of NucleofectorTM electroporator （Amaxa biosystems, Germany） in combination with medium-contained G418 provided a high efficiency of gene delivery for advanced selection. Neucleofected cells were plated on the top of fibronectincoated Petri dishes. Addition of Ly294002 and raised the glucose in medium at 24 h before examination.The differentiation status of these cells was monitored by semi-quantitative PCR （SQ-PCR） detection of the expression of relative genes, such as oct-4, sox-17, foxa2, mixll, pdx-1, insulin 1, glucagons and somatostatin. The percentage of IPC population on d 18 of the experiment was investigated by immunohistochemistry （IHC）, and the content/secretion of insulin was estimated by ELISA assay. The mice with severe combined immunodeficiency disease （SCID） pretreated with streptozotocin （STZ） were used to eliminate plasma glucose restoration after pax4^＋ ES implantation. RESULTS： A high efficiency of gene delivery was demonstrated when neucleofection was used in the present study; approximately 70% cells showed DsRed expression 2 d after neucleofection. By selection of medium-contained G418, the percentage of DsRed expressing cells kept high till the end of study. The pancreatic differentiation seemed to be accelerated by pax4 nucleofection. When compared to the group of cells with mock control, foxa2, mixll, pdxl, higher insulin and somatostatin levels were detected by SQ-PCR 4 d after nucleofection in the group of pax4 expressing plasmid delivery. Approximately 55% of neucleofected cells showed insulin expression 18 d after neucleofection, and only 18% of cells showed insulin expression in mock control. The disturbance was shown by nucleofected pax4 RNAi vector; only 8% of cells expressed insulin 18 d after nucleofection. A higher IPC population was also detected in the insulin content by ELISA assay, and the glucose dependency was demonstrated in insulin secretion level. In the animal model, improvement of average plasma glucose concentration was observed in the group of pax-4 expressed ES of SCID mice pretreated with STZ, but no significant difference was observed in the group of STZ-pretreated SCID mice who were transplanted ES with mock plasmid. CONCLUSION： Enhancement of IPC differentiation from EB-dissociated ES cells can be revealed by simply using pax4 expressing plasrnid delivery. Not only more IPCs but also pancreatic differentiation-related genes can be detected by SQ-PCR. Expression of relative genes, such as foxa 2, mixl 1, pdx-1, insulin 1 and somatostatin after nucleofection, suggests that pax4 accelerates the whole differentiation progress. The higher insulin production with glucose dependent modulation suggests that pax4 expression can drive more mature IPCs. Although further determination of the entire mechanism is required, the potential of pax-4-nucleofected cells in medical treatment is promising.

Biomarker Records of Phytoplankton Productivity and Community Structure Changes During the Last 14000 Years in the Mud Area Southwest off Cheju Island, East China Sea

The ecological environment in the East China Sea(ECS)and the Yellow Sea(YS)has changed significantly due to sea-level rising and the Kuroshio incursion since the last deglaciation.In this study,biomarker records of core F10B from the mud area southwest off Cheju Island(MSWCI)were generated to evaluate phytoplankton productivity and community structure changes in response to environmental evolution during the last 14 kyr.The contents of diatom,dinoflagellate and haptophyte biomarkers(brassicasterol,dinosterol and C37alkenones)display similar trends,with increasing phytoplankton productivity during the last 14kyr due to the increased influences of the Kuroshio,and especially due to the eddy-induced upwelling during the late Holocene.On the other hand,the contents of terrestrial biomarkers(C28+C30+C32n-alkanols)and terrestrial organic matter(TOM)proxies(TMBR′and BIT)all reveal decreasing TOM input into the area around the sampling site for the 14 kyr,mostly due to sea-level ris-ing.Phytoplankton biomarker ratios reveal a shift from a haptophyte-dominated community at 6.2 2.5 kyr BP to a diatom-dominated community at 2.5 1.45 kyr BP,likely caused by a stronger cold eddy circulation system at 2.5 1.45 kyr BP in the MSWCI.

Responses of Primary Productivity to Current and Climate Changes in the Mud Area to the Southwest of Cheju Island During the Past 800 Years

The biogenic silica (BSi) ,total organic carbon (TOC) ,total nitrogen (TN) and grain size were analyzed with a gravity core (3250-6) collected from the mud area in the north East China Sea.The average deposition rate of the upper core was about 0.078cm yr1based on the results of 210Pbex.The mean grain size increased with depth in general.The frequency distribution of grain size showed that two marked changes of deposition environment occurred at 30 cm and 50 cm depths (about 1550 AD and 1300 AD,respectively) .The variations of BSi and TOC indicated two distinct major periods of primary productivity over the past 800 years:a stage of low primary productivity corresponding to weak upwelling and low nutrient input below 30 cm depth (about 1200-1550 AD) ,and a stage of high primary productivity with strong currents and upwelling above 30 cm depth (about 1550-1950 AD) .The stage with high primary productive appeared to be due to the northward-expanded muddy area caused by strong Asian Winter Monsoon and enhanced Yellow Sea Warm Current in winter.In conclusion,the BSi and TOC in the muddy sediments,the symbols of marine primary productivity,can be then used to investigate the evolution history of currents and relative climate change in the offshore areas.

Report of Pontoniinae Shrimps (Crustacea: Decapoda) Collected by Joint Chinese-German Marine Biology Expeditions to Hainan Island, South China Sea. Ⅲ. Periclimenes

The present paper reports 12 species of genus Periclimenes, subfamily Pontoniinae, collected from Hainan Island, South China Sea, by the Joint Chinese-German Marine Biology Expeditions, in which, Periclimenes consobrinus and P. tenuipes are new records from Chinese waters, and Periclimenes amymone, P. holthuisi, P. soror, and P. toloensis are recorded for the first time from Hainan Island.

An Advanced Teaching Lab for the Setting up of an Islanded Production Unit

The present paper addresses an advanced teaching lab consisting of setting up an islanded production unit. This teaching lab takes place in the very last semester at master level for students in electrical engineering with energy specialization. The purpose of this teaching lab is to combine knowledge learned in different areas such as power electronics, control, electrical machines and networks, and make use of all of them in practice. The present paper describes in detail the different steps followed by the student to set up an islanded production unit.

Qingdao Refining Project Kicks off

In June 22, a ground breaking ceremony was warmly held for Qingdao Refining Project. It marked that the construction of Qingdao Refining Project have entered into a fast progressing period, and that the construction of a ten-million-ton refinery “being the first level in the world and taking the lead in China”in the 21st century have been completely launched.

Technology Embedded Imagination for Arab Women

With advancing channels of communication easing the availability of technological tools, the young minds of women in the Arabian Peninsula are ready to adopt forthcoming challenges. The embrace of storytelling in the digital realm by these young minds reflects the interplay of traditional culture, western modernism, and changes brought about by media industries. These women have adopted and used new media as a vehicle to maneuver their interesting and fresh ideas and have seen the rectilinear screen of a computer as an exit through which to come out and say something to the masses. The thinking methodology has culminated in pragmatic and dynamic visuals in order to celebrate, inform, and create awareness about the issues that they see in the region and among the people. With this freedom of expression, they are creating a new visual culture and transforming themselves into a meaningful workforce. With emerging technological tools, they blend intuitive, pragmatic, emotional, and contextual imagination with a highly-sophisticated production pipeline in order to incorporate the challenges of synthesizing ＂dialect＂ and ＂codes＂ that have traditionally been segregated in the culture. Their work is an amalgamation of arts/design, animation, life footage, and meaningful communication. With cyclical evaluation, they entwine the two elements, creativity, and technicality with massive exposure of Digital Assists Management （DAM）. This paper explains how current technological tools and contextualized teaching instructions foster meaningful content with the elicitation and mergence of Arab anthropological characteristics.

Changes in Philornis infestation behavior threaten Darwin＇s finch survival

The conservation behavior framework is useful to identify key linkages between behavior and conservation practice. We apply this framework to a novel host-parasite system on the Galapagos Islands and ask if there have been changes in parasite oviposition behavior and host mortality patterns across the first decade （2004-2013） of its known association. The Dipteran parasite Philornis downsi was first discovered in Darwin＇s finch nests in 1997 and is the biggest threat to the survival of Galapagos land birds. Host mortality has increased over the past decade. In Dipterans, pupation and pupae size are determined by access to host resources. Here, we test the hypothesis that P downsi flies are laying eggs in finch nests earlier in the nestling phase to maximize larval feeding time and therefore chance of pupation success before host death. The results show fewer 1st instar larvae later in the host nesting cycle in support of earlier egg laying behavior by female flies. Between 2004 and 2013, parasite intensity increased from -28 to -48 parasites per nest, host mortality increased from -50% to -90%, and host age at death decreased from -11 to -5 days. The earlier age at host death was correlated with fewer pupae （from -50% to -20%） and smaller pupae size （-10% decrease）. Changes in parasite behavior reveal new fitness costs to both the parasite and Darwin＇s finches. These findings un- derscore the need for urgent conservation action to save Darwin＇s finches from extinction due to a novel, lethal and introduced parasite [Current Zoology 60 （4）： 542-550, 2014].