This review describes the present hot research areas of mangrove-associated fungi, including its biodiversity, ecological roles, novel metabolites productions and biotechnological potential. Mangrove-associated fungi ...This review describes the present hot research areas of mangrove-associated fungi, including its biodiversity, ecological roles, novel metabolites productions and biotechnological potential. Mangrove-associated fungi were divided into saprophytic, parasitic and true symbiotic fungi based on its ecological roles. Saprophytic fungi are fundamental to decomposition and energy flow of mangrove, additionally, their potential toxicity also exists. Pathogenic fungi have significant effects on mangrove survival, growth, and fitness. Endophytic fungi, the most prolific source of diverse bioactive compounds found among that of mangrove-associated fungi, are found in most species of mangroves. Although a significant number of reports focused on the antimicrobial, insecticidal and other bioactive metabolites as well as many novel enzymes isolatcd from mangrove-associated fungi, and many of those metabolites from endophytic fungi are suspected to be of significant to mangrove, only few studies have provided convincing evidence for symbiotic producers in mangrove. Hence, this paper discusses the present progress of molecular methods used to correlate the ecological roles of endophytic fungi with their bioactive metabolites;, meanwhile, the potential of using metabolic engineering and post-genomic approaches to isolate more novel enzymes and bioactive compounds and to make their possible commercial application was also discussed.展开更多
Machining is a mechanical process where excess material from a work is removed to produce a product. At the moment different ferrous, non-ferrous materials and industrial blue wax have been used for prototype models. ...Machining is a mechanical process where excess material from a work is removed to produce a product. At the moment different ferrous, non-ferrous materials and industrial blue wax have been used for prototype models. However such materials is very expensive. Hence an attempt is made to substitute these materials by the palm oil based bio-wax produced in Malaysia. In this research, the authors will analyze and investigate whether there is a possibility to use the palm oil based bio-wax material to substitute with the petroleum based industrial-wax. Experimental analyses are carried out to investigate the capability and the strength of the palm oil based bio-wax material. The matrix blends were prepared of fatty acids from oleo-chemicals, palm oil wax, natural ash fibre and low linear density polyethylene （LLDPE） by stirring and melt-mixing. Sample b!ends are machined with lathe machining process. The sample blends showed there was no built edge formation and good smooth surface production.展开更多
Equipment plays an important role in open pit mining industry and its cost competence at efficient operation and maintenance techniques centered on reliability can lead to significant cost reduction.The application of...Equipment plays an important role in open pit mining industry and its cost competence at efficient operation and maintenance techniques centered on reliability can lead to significant cost reduction.The application of optimal maintenance process was investigated for minimizing the equipment breakdowns and downtimes in Sungun Copper Mine.It results in the improved efficiency and productivity of the equipment and lowered expenses as well as the increased profit margin.The field operating data of 10 trucks are used to estimate the failure and maintenance profile for each component,and modeling and simulation are accomplished by using reliability block diagram method.Trend analysis was then conducted to select proper probabilistic model for maintenance profile.Then reliability of the system was evaluated and importance of each component was computed by weighted importance measure method.This analysis led to identify the items with critical impact on availability of overall equipment in order to prioritize improvement decisions.Later,the availability of trucks was evaluated using Monte Carlo simulation and it is revealed that the uptime of the trucks is around 11000 h at 12000 operation hours.Finally,uncertainty analysis was performed to account for the uncertainty sources in data and models.展开更多
Phospholipase is an enzyme that hydrolyzes phospholipids releasing a variety of products, like for example lyso-phospholipids, free fatty acids, di-acylglycerols, choline phosphate and phosphatidates, depending on the...Phospholipase is an enzyme that hydrolyzes phospholipids releasing a variety of products, like for example lyso-phospholipids, free fatty acids, di-acylglycerols, choline phosphate and phosphatidates, depending on the site of hydrolysis. In cheese production, lysophospholipids act as surface-active agents in the cheese curd, helping emulsification of water and fat during processing and reducing syneresis. Phospholipases are more specific and have little or no activity toward di- or triglycerides. As a result of phospholipid hydrolysis, flavor defects do not occur due to the main formation of palmitic, oleic, and stearic acids, which are non-volatile short chains fatty acids. According to the scientific studies the use of phospholipase is able to increase the yield of cheese and reduce the environmental impacts of cheese production. Protein and fat largely determine cheese yield. Depending on the milk composition, 75% to 78% of milk protein and 85% to 95% of milk fat are entrapped in the cheese curd. The remaining protein and fat are lost in the whey and, to a lesser extent, in the brine. Crucially in the production of pasta filata cheese fat losses occur in the hot stretching step, where the fresh curd is molded and stretched in hot water. The lysophospholipid-casein complexes should be studied to understand the mechanism leading to cheese yield improvements.展开更多
Peformance enhancing drugs are widely used today in different sport fields, and therefore the successful anti-doping activity should be based not only on regular doping controls, but also it should show the real alter...Peformance enhancing drugs are widely used today in different sport fields, and therefore the successful anti-doping activity should be based not only on regular doping controls, but also it should show the real alternative： how to replace the forbidden doping substances with legal and effective supplements. The paper deals with application proposition of protein concentrates, free AA （amino acids）, HMB （hydroxy-methyle-butyrate）, creatine and camitine. If the athlete is involved in strength and power sport （e.g. throwing events in track and field, olympic weightlifting etc.）, the explosive and maximum strength is of primary importance. For strength athletes, these mentioned substances are those legal preparates, supplements, which can help effectively in performance improvement of sport results. The main reason is that using protein concentrates, AAs, HMB, creatine and camitine, the anabolic （and anticatabolic） effect will enhance the protein biosynthesis of the organism, improve the aerob and anaerob capacity of the athletes, activate and stimulate the own hormonal system. And the final result is creating higher loadability because of the faster recovery, and higher performance level on the competition. Of course, the athletes--as a minimum requirement--need an adequate nutrition （good balanced nourishment）, as well, with appropriate application and supplementation of all vitamins and essential minerals.展开更多
Biologically produced surfactants （SACs） can mobilize and solubilize non-aqueous phase liquids （NAPL） adsorbed onto soil constituents. The interest in microbial surfactants has increased during recent years due to...Biologically produced surfactants （SACs） can mobilize and solubilize non-aqueous phase liquids （NAPL） adsorbed onto soil constituents. The interest in microbial surfactants has increased during recent years due to their lower toxicity, higher biodegradability, selectivity and specific activity under extreme conditions than synthetic SACs. Main output of the project represents preparation of this yeast biosurfactant intended for washing of matrices contaminated by NAPL. The influence of cultivation media composition on biosurfactant production was studied and basic properties （critical micelle concentration （CMC）, minimum surface tension） of isolated biosurfactants were compared with properties of synthetic surfactant with surface tension measurement. The interracial tension of the systems containing aqueous solutions of different concentrations and non-polar substances was measured with petroleum compounds （kerosene Jet A-l）, aromatic and aliphatic hydrocarbons （represented by toluene and hexane）. The solution of biosurfactant Yarrowia lipolytica （YAR） in the concentration range of 0-500 mg/L reduced interracial tension by 80% in all representative systems with model contaminants; biosurfactant Candida bombicola （CAN） was less efficient. Solubilization properties were proved with toluene and hexachlorocyclohexane （HCH） isomers alpha and gamma, and effective concentration of biosurfactants was determined as 100 mg/L for toluene and HCH. SACs produced by lipophilic yeast with non-toxic and non-pathogenic status （Yarrowia lipolytica, Candida sp., etc.） seem to be very promising. The results obtained will be used for the application of biosurfactants in the clean-up technologies as agents for the mobilization of non-polar contaminants as well as for stimulation of bioremediation processes.展开更多
基金Foundation project： This work was supported partly by the Guangzhou Natural Science Foundation （Grant No. 2007Z3-EO581）, the Guangdong Provincial Natural Science Foundation （Grant No. 2007A0200300001-7 05003268）, the Chinese High-Tech 863 Project （Grant No. 2006AA09Z422）, and the National Natural Science Foundation of China （Grant No. 20572136）.
文摘This review describes the present hot research areas of mangrove-associated fungi, including its biodiversity, ecological roles, novel metabolites productions and biotechnological potential. Mangrove-associated fungi were divided into saprophytic, parasitic and true symbiotic fungi based on its ecological roles. Saprophytic fungi are fundamental to decomposition and energy flow of mangrove, additionally, their potential toxicity also exists. Pathogenic fungi have significant effects on mangrove survival, growth, and fitness. Endophytic fungi, the most prolific source of diverse bioactive compounds found among that of mangrove-associated fungi, are found in most species of mangroves. Although a significant number of reports focused on the antimicrobial, insecticidal and other bioactive metabolites as well as many novel enzymes isolatcd from mangrove-associated fungi, and many of those metabolites from endophytic fungi are suspected to be of significant to mangrove, only few studies have provided convincing evidence for symbiotic producers in mangrove. Hence, this paper discusses the present progress of molecular methods used to correlate the ecological roles of endophytic fungi with their bioactive metabolites;, meanwhile, the potential of using metabolic engineering and post-genomic approaches to isolate more novel enzymes and bioactive compounds and to make their possible commercial application was also discussed.
文摘Machining is a mechanical process where excess material from a work is removed to produce a product. At the moment different ferrous, non-ferrous materials and industrial blue wax have been used for prototype models. However such materials is very expensive. Hence an attempt is made to substitute these materials by the palm oil based bio-wax produced in Malaysia. In this research, the authors will analyze and investigate whether there is a possibility to use the palm oil based bio-wax material to substitute with the petroleum based industrial-wax. Experimental analyses are carried out to investigate the capability and the strength of the palm oil based bio-wax material. The matrix blends were prepared of fatty acids from oleo-chemicals, palm oil wax, natural ash fibre and low linear density polyethylene （LLDPE） by stirring and melt-mixing. Sample b!ends are machined with lathe machining process. The sample blends showed there was no built edge formation and good smooth surface production.
基金the support of the Maintenance Department of Mobin Co.Sungun Copper mine
文摘Equipment plays an important role in open pit mining industry and its cost competence at efficient operation and maintenance techniques centered on reliability can lead to significant cost reduction.The application of optimal maintenance process was investigated for minimizing the equipment breakdowns and downtimes in Sungun Copper Mine.It results in the improved efficiency and productivity of the equipment and lowered expenses as well as the increased profit margin.The field operating data of 10 trucks are used to estimate the failure and maintenance profile for each component,and modeling and simulation are accomplished by using reliability block diagram method.Trend analysis was then conducted to select proper probabilistic model for maintenance profile.Then reliability of the system was evaluated and importance of each component was computed by weighted importance measure method.This analysis led to identify the items with critical impact on availability of overall equipment in order to prioritize improvement decisions.Later,the availability of trucks was evaluated using Monte Carlo simulation and it is revealed that the uptime of the trucks is around 11000 h at 12000 operation hours.Finally,uncertainty analysis was performed to account for the uncertainty sources in data and models.
文摘Phospholipase is an enzyme that hydrolyzes phospholipids releasing a variety of products, like for example lyso-phospholipids, free fatty acids, di-acylglycerols, choline phosphate and phosphatidates, depending on the site of hydrolysis. In cheese production, lysophospholipids act as surface-active agents in the cheese curd, helping emulsification of water and fat during processing and reducing syneresis. Phospholipases are more specific and have little or no activity toward di- or triglycerides. As a result of phospholipid hydrolysis, flavor defects do not occur due to the main formation of palmitic, oleic, and stearic acids, which are non-volatile short chains fatty acids. According to the scientific studies the use of phospholipase is able to increase the yield of cheese and reduce the environmental impacts of cheese production. Protein and fat largely determine cheese yield. Depending on the milk composition, 75% to 78% of milk protein and 85% to 95% of milk fat are entrapped in the cheese curd. The remaining protein and fat are lost in the whey and, to a lesser extent, in the brine. Crucially in the production of pasta filata cheese fat losses occur in the hot stretching step, where the fresh curd is molded and stretched in hot water. The lysophospholipid-casein complexes should be studied to understand the mechanism leading to cheese yield improvements.
文摘Peformance enhancing drugs are widely used today in different sport fields, and therefore the successful anti-doping activity should be based not only on regular doping controls, but also it should show the real alternative： how to replace the forbidden doping substances with legal and effective supplements. The paper deals with application proposition of protein concentrates, free AA （amino acids）, HMB （hydroxy-methyle-butyrate）, creatine and camitine. If the athlete is involved in strength and power sport （e.g. throwing events in track and field, olympic weightlifting etc.）, the explosive and maximum strength is of primary importance. For strength athletes, these mentioned substances are those legal preparates, supplements, which can help effectively in performance improvement of sport results. The main reason is that using protein concentrates, AAs, HMB, creatine and camitine, the anabolic （and anticatabolic） effect will enhance the protein biosynthesis of the organism, improve the aerob and anaerob capacity of the athletes, activate and stimulate the own hormonal system. And the final result is creating higher loadability because of the faster recovery, and higher performance level on the competition. Of course, the athletes--as a minimum requirement--need an adequate nutrition （good balanced nourishment）, as well, with appropriate application and supplementation of all vitamins and essential minerals.
文摘Biologically produced surfactants （SACs） can mobilize and solubilize non-aqueous phase liquids （NAPL） adsorbed onto soil constituents. The interest in microbial surfactants has increased during recent years due to their lower toxicity, higher biodegradability, selectivity and specific activity under extreme conditions than synthetic SACs. Main output of the project represents preparation of this yeast biosurfactant intended for washing of matrices contaminated by NAPL. The influence of cultivation media composition on biosurfactant production was studied and basic properties （critical micelle concentration （CMC）, minimum surface tension） of isolated biosurfactants were compared with properties of synthetic surfactant with surface tension measurement. The interracial tension of the systems containing aqueous solutions of different concentrations and non-polar substances was measured with petroleum compounds （kerosene Jet A-l）, aromatic and aliphatic hydrocarbons （represented by toluene and hexane）. The solution of biosurfactant Yarrowia lipolytica （YAR） in the concentration range of 0-500 mg/L reduced interracial tension by 80% in all representative systems with model contaminants; biosurfactant Candida bombicola （CAN） was less efficient. Solubilization properties were proved with toluene and hexachlorocyclohexane （HCH） isomers alpha and gamma, and effective concentration of biosurfactants was determined as 100 mg/L for toluene and HCH. SACs produced by lipophilic yeast with non-toxic and non-pathogenic status （Yarrowia lipolytica, Candida sp., etc.） seem to be very promising. The results obtained will be used for the application of biosurfactants in the clean-up technologies as agents for the mobilization of non-polar contaminants as well as for stimulation of bioremediation processes.
