生淀粉糖化酶产生菌Rhizopus OR-1UVN培养基优化的研究

通过对菌株RhizopusOR 1UVN培养基优化 ,确定最佳发酵培养基是 :50g玉米粉 ,30g豆粕粉 ,4gNaNO3,1 0g麸皮 ,0 .5gMgSO4 ·7H2 O ,0 .5gZnSO4 ,1gK2 HPO4 ,1L水 ,从该菌株发酵过程曲线 ,发现大量产酶期在 40～ 48h ,生淀粉糖化酶量与熟淀粉糖化酶量的比率(RDA)为 0

宇宙中可见物质质量起源的接触相互作用模型探究

关于我们所处的宇宙,尚有许多深刻且未知的问题,在这些问题中,可见物质质量起源的研究尤为基本且意义深远.这些可见物质质量绝大部分都由一个单一的质量量级描述,也就是质子的质量m_(p).随着希格斯(Higgs)粒子的发现,我们已经能够充分解释可见物质中≤1%的“基本”粒子的质量起源,即通过希格斯机制(Higgs Mechanism)获得的质量.根据现有的理论,剩下≥99%的质量是标准模型中强相互作用的一个特有的特征——胶子自相互作用的结果,也就是演生强子质量产生机制(Emergence of Hadronic Mass,EHM).通过一个接触相互作用模型,研究了流夸克质量、有效胶子质量、量子色动力学(Quantum Chromodynamics,QCD)的跑动耦合常数、红外截断和紫外截断对组分夸克质量的影响,探讨这些量的改变对可见物质质量的大小或者宇宙中可见物质呈现形式的影响,为现在的宇宙描述了更多可能的存在形式,加深了我们对宇宙在这些量上取特定值的理解.

Techno-economic and Environmental Assessments of SAF from Bioamss:An Review

This review presents a comprehensive techno-economic and life-cycle assessment of sustainable aviation fuel(SAF)production from biomass.The critical need for transitioning towards environmentally sustainable alternatives for liquid fuel and aviation industry is first discussed.Key insights encompass the evolutionary progression of biofuel production from first-generation to second-generation biofuels,with a focus on utilizing non-food sources like woody biomass for enhanced sustainability.Available data from the literature on techno-economic assessments of various SAF production pathways are analyzed including production costs,conversion efficiency,and scalability.Moreover,results of lifecycle assessments associated with different SAF production pathways are presented,providing essential insights for decision-making processes.The challenges of scaling up woody biomass-based SAF production are discussed based on the assessment results,and recommendations are proposed to steer stakeholders towards a greener and more sustainable trajectory for aviation operations.

Interannual Variation in Terrestrial Ecosystem Carbon Fluxes in China from 1981 to 1998

A dynamic biogeochemical model was used to estimate the responses of China's terrestrial net primary productivity (NPP), soil heterotrophic respiration (HR) and net ecosystem productivity (NEP) to changes in climate and atmospheric CO2 from 1981 to 1998. Results show that China's total NPP varied between 2.89 and 3.37 Gt C/a and had an increasing trend by 0.32% per year, HR varied between 2.89 and 3.21 Gt C/a and grew by 0.40% per year, Annual NEP varied between -0.32 and 0.25 Gt C but had no statistically significant interannual trend. The positive mean NEP indicates that China's terrestrial ecosystems were taking up carbon with a total carbon sequestration of 1.22 Gt C during the analysis period. The terrestrial NEP in China related to climate and atmospheric CO2 increases accounted for about 10% of the world's total and was similar to the level of the United States in the same period. The mean annual NEP for the analysis period was near to zero for most of the regions in China, but significantly positive NEP occurred in Northeast China Plain, the southeastern Xizang (Tibet) and Huang-Huai-Hai Plain, and negative NEP occurred in the Da Hinggan Mountains, Xiao Hinggan Mountains, Loess Plateau and Yunnan-Guizhou Plateau. China's climate at the time was warm and dry relative to other periods, so the estimated NEP is probably lower than the average level. China's terrestrial NEP may increase if climate becomes wetter but is likely to continue to decrease if the present warming and drying trend sustains.

Status of urban vegetation in Guangzhou City

According to a survey for the urban vegetation of Guangzhou, urban vegetation has a significantly difference from natural vegetation because of intense human impacts. The research was conducted in a synthetic survey for soil, species di-versity, roadside trees and ecological function of urban vegetation in Guangzhou City. The results showed that: (1) soil densi-ties of urban roadside and park forests were higher than mean density of natural forest soil. The pH values of soil in urban roadside were higher too, and the content of organic matter and the concentration of nitrogen were lower. (2) Species diversity of urban vegetation was lower. The most number of species was only 16 species in tree layers of urban forest. (3) Tree growth was limited by narrow space in high-density urban area, where the trees with defects and disorders were common. (4) Com-paring with mature natural forests, the productivity of urban vegetation was lower. The effect of urban vegetation on balance of carbon and oxygen were influenced by the low primary production of urban vegetation. Therefore, the growth condition for urban vegetation should be improved. Biodiversity, primary production and ecological function should be increased for urban vegetation in order to improve urban eco-environment.

Effects of Milk Replacer on the Performance and Blood Biochemical Indices in Lambs

[Objective] In this study, the performance and blood biochemical indices in lamps fed on milk replacer were researched, the feasibility of early weaned in lamps was discussed. [Method] 37 of 10 days lambs of Suffolk（♂） x XiaoWeiHanYang（ ♀ ） were randomly divided into group A, B, C, D for this experiment. Group A, B, C were fed by 3 milk-replacers containing milk for different protein source respectively, and ten Iambs in each group; group D （seven lambs） were fed by ewes as the control. The performance and blood biochemical indices of the lamps were determined. [ Result] The results showed that the average daily gains of 10 - 15 days of age was not significantly different between group A and D （ P 〉 O. 05 ）, the control were higher significandy than the group B,C （P 〈0.05）. The average daily gains of 16 -25, 26 -35 days of age were not significantly different in group A, B, C, D. The daily weight gains of Group A, B, C in 45 - 55 days of age were higher significantly than the control （P〈0.05 ）. Plasma urea nitrogen （PUN） of group B and C were higher than group A significantly （P〈0.05 ） ;Total protein （ TP）, Plasma albumin （ALB） and Plasma total amino acid were no significant difference among the other 3 groups （P〉0.05 ）. [Conclusion] So, the milk-replacers were available for early-weaned lamb.

Assessment of Climate for Agricultural Suitability and Optimal Allocation of Agricultural Production in the Guanzhong Region, Shaanxi Province

Based on spatial climatic data of agriculture and the experiment data, the models of agro-ecological assessment of climate for agricultural suitability in this study were developed using the fuzzy mathematical method. Three coefficients, in- cluding the resource coefficient （Cr）, the efficiency coefficient （Ce）, and the utility co- efficient （K）, were used in the models, which were calculated based on temperature, moisture, and sunshine duration data of Guanzhong region, Shaanxi Province. The results indicated that resource coefficient was higher in west of the region than that in east, and higher in south （especially in the Central Shaanxi Plain） than that in the Weibei plateau. The value of Cr changed from 6.5 to 9.2 from north to plain area. Spatial change of efficiency coefficient was obvious, lower in the northeast than in the central plain, and the value of Ce changed from 2.3 to 6.5 from the northeast to the central plain. As for utility coefficient, it was lower in northeastern part of the Weibei plateau and in southern mountain areas than that in the central plain, showing significant latitudinal zonality. Furthermore, the value of K increased from 0.35 to 0.78 from northeast to the central plain, and decreased from 0.78 to 0.53 from the central plain to southern mountain areas. These indicated that climate resource in the central plain region was more abundant and potential, compared with other regions. GuanZhong region was classified into three larger agricultural zones and three small independent zones, according to agro-ecological assessment. Light, heat and water resources should be made use of in an efficient way in spatial allo- cation of agricultural production. For example, water facilities should also be im- proved in Weibei plateau region where highly-qualified fruit should be enhanced and fruit processing industrial chain should be shaped. Large-scale production area of wheat should be increased in central irrigation region and more vegetable bases should be developed around large and medium-scale cities. Thanks for outstanding water conservation function, the three-dimensional agriculture including medicine and other sideline production should be developed in Qinling Mountains and the special- ized commercial agriculture should be accelerated in independent small zones, ac- cording to local conditions. In the research, different crop varieties were developed in corresponding regions as per current eco-climatic conditions.

Establishment of Optimized Eco-productive Paradigm in the Farming-Pastoral Zone of Northern China

The farming-pastoral zone of northern China plays a dual role in ecological conditions and production and occupies an important position in the national economy. In this paper, the methodology of system engineering is introduced to construct and optimize an eco-productive paradigm system for the typical areas of the farming-pastoral zone. The system was constructed in the following steps: (1) design the framework of the paradigm system based on the data of physical site characteristics, biocommunities, production and economy, social culture and historical changes; (2) analyze the vegetation patterns, the interactions between vegetation and environmental factors (natural, social, economic, etc.) and the contributions of vegetation to the area, including the synthesis of the existing researches; and (3) provide the spatial arrangements of ecosystems and planning of each area, raise a comprehensive indicators of evaluation, evaluate the feasibility and soundness, and determine the optimum eco-productive paradigms for policy-makers which were land-use patterns within the threshold of ecological conservation. Three primitive paradigms of Maowusu (Mu Us) Ssandy Land, Loess Plateau and Huailai Basin in the farming-pastoral zone of northern China were proposed according to this paradigm system.

Effects of Grassland Eco-Protection Compensation and Reward System

Grassland ecological protection compensation and reward policy is the largest-scale investment concerning themost extensive areas since foundation of the PRC. It will be the long-term implementationpolicy for grassland ecological protection. In this study,based on macro-perspective, the policy effects ofgrasslandproductivity, ecological protection, animal husbandryoutput, pastoralists＇ income were ana- lyzed. The resultsshow that, afterimplementation of the policy, naturalgrass production and grasslandtheoretical stocking rateincreased. The averagenatural grasslandlivestockoverloading ratedecreased significantly, comprehensivenationalgrasslandvegetation coverageis increasing. Besides, adult cattleandbeef yield arefluctuated. Sheep head, adult sheep, sheep production, milk productionincreasedin varying degrees. The per capita netincomeof farmers and pastoralists, livestock income, the proportion oflivestockincomewere higher than those beforeimplementation of the policy.

A Summary of Ratoon Rice Production Technology in Southwest Ecotope of China

This paper focus on the ratoon rice, which with the features of south Chi-na paddy cultivation technology. First, the development of the technology research on ratoon rice in southwest ecotope of China was briefly reviewed. Second, the key technical measures of the ratoon rice production were described in detail. At last, the application effect of the ratoon rice production technology was described accu-rately, which based on the production practice in recent years. The purpose of this article is promoting the research and extension of the ratoon rice technology more widely in world’s main rice-producing areas, especial y in the suitable area where the light and heat resources are rich.

Screening of a Novel Bioflocculant-producing Strain and Research on Its Flocculation

[Objective] This study aimed to screen a bacterial strain capable of producing bioflocculant. [Method] A bacterial strain T-11 capable of producing bioflocculant was isolated from activated sludge. Detailed tests on the morphological, physiological and biochemical characteristics were carried out and identification was performed to identify the strain. Finally, the bioflocculant was isolated and purified, and the flocculating activity and chemical characteristics were measured. [Result] It was identified as Serratia plumuthica based on its morphological, physiological and biochemical characteristics. This strain secreted flocculant best in a culture medium which included sucrose and NaNO3. The maximal cell growth was achieved within 10 h and the flocculating activity paralleled to it. It was found to be effective for flocculation of kaolin suspension, when added at a final concentration of 0.7 mg/L, over a range of pHs （2-7）, and temperature （approximately 30-80 ℃）. Chemical analysis indicated that the bioflocculant was an acidic polysaccharide consisting of glucose, glucuronic acid and galactose, talose and altrose. Infrared spectrum analysis also revealed typical characteristics of polysaccharides. [Conclusion] The biofloccu- lants produced by strain T-11 can greatly improve the ability of activated sludge to settle.

Effects of FCMP Compound Fertilizer on Development and Yield of Early Rice

[Objective] The aim was to explore effects of FCMP compound fertilizer on growth, development and yield of early rice. [Method] Based on lower-graded phos- phate resource, effects of FCMP compound fertilizers on yield and growth of early rice in a field were researched. [Result] FCMP compound fertilizers 0, 1 and 2 en- hanced rice yield, increasing by 21.86%, 20.25% and 13.46%, compared with the rice applied with conventional fertilizer; number of productive ears and grain number per ear in unit area were improved by FCMP compound fertilizer, for example, the two factors achieved the highest with FCMP compound fertilizer 1, increasing by 11.70% and 19.63%. Furthermore, FCMP compound fertilizer promoted plant height and tiller number, maintained high photosynthetic efficiency, enhanced lodging-resis- tance and guaranteed stable and high yield. [Conclusion] The research is of theoret- ical and practical significance for further exploration of production techniques and application of FCMP compound fertilizer.

Discussion on Action and Potential of Fenlong Megascience in the Symbiosis between Human and Nature

The fourth new farming model Fenlong is identified as megascience for the first time. Fenlong can be directly applied to farming of farmland, remoulding of mortar black soil and saline alkali land and development of degraded grassland. Deep loosening can create huge soil reservoirs, reduce fertilization, promote indi- rectly the improvement of river water fisheries and water sources and the upgrading of shaping and hydropower industry, thus making a new round of mobilization and pooling of natural resources. As a result, the nature is able to produce good food needed by human, the spatial dimension of the land is increased, the natural pre- cipitation storage is increased, the flood and drought disasters are reduced, the eco-environment is improved, and the economic benefits are increased. Fenlong is not restricted to global application by ecological region and crop variety and is not subject to the time-space constraints for a hundred thousand years. On the basis of utilizing the heaven and earth resources, it brings about a huge effect of mega- science. Compare with traditional farming, the depth under the mode of Fenlong is increased by 2-3 times, the contents of nutrient, water, oxygen and microorganism in the soil are increased by 10%-200%, the content of pale salt is increased by 20%-40%, the temperature is increased by 2-4 ~C, and the photosynthetic efficiency of crops is improved by 10%. Under the cultivation mode of Fenlong, the yield of crop applied with no fertilizers is increased by more than 10%, crop yield is still in- creased by more than 5% when the application amount of chemical fertilizer is re- duced by 10%-20%. Under the farming mode of Fenlong combined with no in- crease in fertilization, the crop yield, crop quality, farming efficiency, natural precipi- tation storage and air humidity are increased by 10%-50%, 5%, 15%, 100% and 5%, respectively, and the emissions of methane and other gases are reduced by more than 5%. Even in mortar black soil, saline alkali land and degraded grassland, the yield is still increased by 15%-50%. These improvement effects can last for many years, helping achieve the real harmonious coexistence between human and nature.

Effect of Chitinase-Producing Strain V-8 on Controlling Cotton Fusarium Wilt

[Objective] This study aimed to screen endophytic bacteria which is antag- onistic to cotton Fusarium wilt. [Method] Fresh cotton plants collected from cotton- growing areas in Jingzhou City, Hubei Province were used as experimental materials to isolate endophytic bacteria. Through chitinase test and co-culturing both micro-or- ganisms side by side on the same PDA culture plate, antagonistic strains to cotton Fusarium wilt were screened. [Result] A total of 83 bacterial isolates were obtained from cotton plants grown in the fields, six of which were chitinase-productive bacte- ria. Through chitinase test and co-culturing both micro-organisms side by side on the same PDA culture plate, strain V-8 which had the strongest antagonistic effect on Fusarium oxysporum f. sp. vasinfectum was screened. Strain V-8 had a wider anti- fungal spectrum with certain inhibitory effect on all the six important pathogenic fungi including Fusarium oxysporum f. sp niveum; it colonized stably in the rhizospheric soil of cotton, with a colonization density of up to 6.2x10s cfu/g fifty days after inoc- ulation; the relative effect on controlling cotton Fusarium wilt in pot test was 73.2%. The Findings of this study suggested that strain V-8 had great potential for biological control of cotton Fusarium wilt and could be taken as a substantial material for the cloning of chitinase genes. [Conclusion] The results from this study provides bases for the control of cotton fusarium wilt, as well as the exploitation of endophytic bac- teria resources in cotton and the development of novel biological pesticides.

Application Effect of Cassava Starch Anaerobic Fermentation Liquid on Watermelon Production

In order to find out a new way for environment-friendly and resourcelized utilization of cassava starch processing wastewater, the cassava starch anaerobic fermentation liquid was applied in watermelon production, and its effects on the growth and development, yield and fruit quality of watermelon were investigated. The results showed that the cassava starch anaerobic fermentation liquid significant- ly promoted the vegetative and reproductive growth and improved the yield and fruit quality of watermelon. Compared with conventional fertilization, the application of cassava starch anaerobic fermentation both with COD concentration of 1 200 mg/L according to the amount of 150 t/hm2 promoted the growth of vines and leaves of watermelon plants, brought forward the flowering, fruiting and harvest of watermelon and significantly increased the fruit number, fruit weight, yield, fruit size, fruit shape index, soluble solid content, soluble sugar content, soluble protein content and Vita- min C content of watermelon. At the same time of improving the yield and quality of watermelon, cassava starch anaerobic fermentation liquid was turned into treasure as a liquid fertilizer. This study provides a new ideal for the yield and quality im- provement of watermelon and the wastewater treating of starch factories.

Regional Division of Production and Development Strategy of Citrus in Hunan Province

[Objective] The aim was to research regional division of citrus production and development strategies in Hunan Province. [Method] According to Rural Statisti- cal Yearbook in Hunan Province during 2002-2011, Citrus in Hunan province were divided into three production regions, namely, superior region, sub-superior and non- superior region. On the base of the divisions, the ecological regionalization and brand strategy, Optimization of regional distribution and developing strategy were proposed, with consideration of avoiding frozen zones, in this paper. [Result] Fresh and processing bases of mandarin orange （C.unshiu Marc）, and specialty industries of seedless ponkan（C.reticulata Blanco）, Bingtang orange （C.sinensis Osbeck Bing- tangcheng）, Dayongjuhuaxinyou （Cgrandis （L.） Osbeck Dayongjuhuaxinyou） and An- jiangxiangyou （C.grandis （L.） Osbeck Anjiangxiangyou） should be constructed, where fresh fruit is dominant, supplemented by canned fruit and juice. Industry belt of sat- suma orange, fresh or processing food, is mainly built, for proportion of early and earlier ripe satsuma orange is over 50% of total yield in Xiangzhong citrus zone; in- dustry belt of excellent fresh navel orange and processing sweet orange should be highlighted in Xiangnan. [Conclusion] The research provides references for decision- making for governments, especially on optimization of citrus production regions and development of citrus industry.

Study on the Variation of Ningxia Climate Productive Potential under the Returning Farmland to Forestland and Grassland Background

The key zones of returning farmland to forestland and grassland in Ningxia were studied. By using the ＂stepwise revised method＂,the climate productive potential,light and temperature productive potential in the zone in recent 50 years were counted. The light and temperature productive potential of corn in Ningxia irrigated area,the central arid zone and the southern mountain area presented the linear increase trend. But when considered the climate productive potentials of light,temperature and water,the numerical value was very low because of the scarce rainfall,and no agriculture without the irrigation. The light and temperature productive potential,climate productive potential of winter wheat in the central arid zone had no significant trend,but the variation range of climate productive potential was very big. The light and temperature productive potential of winter wheat in the southern mountain area had no significant variation trend,and the climate productive potential presented the weak decline trend. It illustrated that the productive of winter wheat was greatly restricted by the water content. By using the meteorological factor data which were simulated by RegCM3-WOFOST/LINGRA coupled model,the future climate productive potentials of winter wheat in the central south of Ningxia was counted. They both presented the weak increase trend. It illustrated that the climate in Ningxia was favorable to improve the yield of winter wheat after returning farmland to forestland.

Effects of Nitrogen Fertilizer Management on Biological Traits and Physiological Indexes at Seedling Stage and Yield of Directly-sown Rapeseed(Brassica napus L.)

Field experiments were carried out to study the effects of different nitroge- napplication rates and application times on biological traits and physiological indexes of directly-sown rapeseed （Brassica napus L.） at the seedling stage and investigate the relationship between these biological traits or physiological indexes at the seedling stage and yield, so as to provide scientific theoretical support for high yield and efficient fertilization management in production of winter rapeseed. Field trials were conducted in Chengdu plain of Sichuan Province under rice-rapeseed rotation system during the period of 2011-2012. The nitrogen application rate trial consisted of five nitrogen levels （0, 90, 180, 270 and 360 kg/hm2） and the nitrogen application time trial included NTl（single application as base fertilized）, NT2 （bottom application ＋one time of topdressing at seedling stage） and NT3 （bottom application＋two times of topdressing at seedling stage） under the same nitrogen rate （225 kg/hm2）. The results indicated that compared with no nitrogen application （NO） treatment, the in- crease of nitrogen fertilizer is beneficial to the increase of biological traits including plant height, green leaf number, leaf area index and dry weight of rapeseed at the seedling stage, the improvement of physiological indexes including total nitrogen content, chlorophyll content and soluble protein content of functional leaves, and the reduction of soluble sugar content. Nitrogen rate was linearly correlated with various biological traits at the seedling stage and physiological indexes including total nitro- gen content, chlorophyll content and soluble sugar content in functional leaves over- a/I, but in parabolic correlation with soluble protein content. Under the same nitrogen rate, NT2 treatment exhibited biological traits remarkably or significantly higher than NT1 treatment and NT3 treatment. The nitrogen application times were linearly cor- related with the physiological indexes of functional leaves at the seedling stage. The various biological traits and physiological index of functional leaves at the seedlings stage were in quadratic function parabolic correlation with seed yield, and the corre- lation was significant （P〈0.05）. Therefore, under the rice-rapeseed rotation system in Chengdu plain, the economic rational nitrogen rate is 180-225 kg/hm2, and the mode of bottom application ＋ one time of topdressing （NT2） is suitable.

Prospects for Study on High-yielding Cultivation Techniques of Super Hybrid Rice

Great progresses have been made in super hybrid rice in China. From the perspectives of dry matter production, nutrient absorption, sink and source, pho-tosynthesis, graln fiI ing and roots, the eco-physioIogical characteristics and high-yielding cuItivation techniques of super hybrid rice in China were discussed. In addi-tion, the probIems that restricted the high and stabIe yielding of super hybrid rice were analyzed, and the deveIopment directions of high-yielding cuItivation techniques for super hybrid rice were also discussed.

Biomass, litterfall and the nutrient fluxes in Chinese fir stands of different age in subtropical China

Chinese fir (Cunninghamia lanceolata (Lamb.) Hook.), a fast-growing, ever-green conifer tree with high yield and excellent quality, is the most important tree species of timber plantations in subtropical China. We investigated the characteristics of biomass, litterfall and nutrient fluxes in the 8, 14 and 24 year-old stands, representing the young, middle-aged and mature stands. The results showed that Chinese fir plantations in central Fujian province had high productivity, and the proportion of stem mass in total biomass was between 50%-70%. Chinese fir was low nutrient-return tree species with litterfall. Nutrient withdrawal from senescing needles was a strong age-dependence for nitrogen, phosphorous and potassium in Chinese fir. With a management system of such short-rotation and continuously pure-crop planting, harvesting timber can lead to great nutrient loss, which may be one of the causes for site degradation.