Microbial transformation of gastrodin by Mucor spinosus strain 3.3450, resulted in a product with a transformation rate close to 100 per cent. This product was identified as p-hydroxy benzyl alcohol on the basis of it...Microbial transformation of gastrodin by Mucor spinosus strain 3.3450, resulted in a product with a transformation rate close to 100 per cent. This product was identified as p-hydroxy benzyl alcohol on the basis of its 1H, 13C NMR and EI-MS spectral data. It could be inferred that the enzyme responsible for the biotransforma-tion reaction was a kind of extracellular and constitutive enzyme since the transformation reaction of the substrate could be carried out in cell free extracts of the fermentation broth of the Mucor spinosus.展开更多
The effects of the tree species and the dosages of Armillariella mellea rhizomoph wood on asexual propagation of wild Gastrodia elata Bl. f. glauca S. Chow were studied in Ganzi prefecture. The results showed that tre...The effects of the tree species and the dosages of Armillariella mellea rhizomoph wood on asexual propagation of wild Gastrodia elata Bl. f. glauca S. Chow were studied in Ganzi prefecture. The results showed that tree species had a very significant effect on asexual reproduction yield of wild Gastrodia elata Bl. f. glauca S. Chow. The drying rate and yield of Gastrodia elata Bl. f. glauca S. Chow cultured by close grained woods such as Quercus spinosa David ex Fr. was extremely significant higher than that cultured by coarse grained wood such as Betula platyphylla Suk. The dosage had a very significant effect on the yield of wild Gastrodia elata Bl. f. glauca S. Chow whereas it had no influence on the drying rate.展开更多
[Objective] The influences of different habitats on asexual propagation of wild Geg(abbreviation for Gastrodia elata Bl. f. glauca S. Chow) being domesticated in Ganzi prefecture was studied. [Method] The research tri...[Objective] The influences of different habitats on asexual propagation of wild Geg(abbreviation for Gastrodia elata Bl. f. glauca S. Chow) being domesticated in Ganzi prefecture was studied. [Method] The research trial was carried out in the following three kinds of habitats at the same time: the plastic greenhouses, the birch forest of shady slope and the shrubbery of sunny slope, and the results were analyzed with group data average hypothesis test method. [Result] The overall tuber(the juvenile tuber, the immature tuber and the mature tuber) yield and the mature tuber yield were both very significantly higher in the plastic greenhouses than in the other two types of habitats, and respectively reached 9.52 and 6.70 kg/m2; the mature tuber drying rate was dramatically or very dramatically lower in the plastic greenhouses than in the other two types of habitats, and was 23.84%; the stabilities of the overall tuber yield, the mature tuber yield and the drying rate were all reduced in the order of greenhouses, birch forest and shrubbery; and the dry mature tuber yield of the plastic greenhouses was the highest in the three kinds of habitats, and was 1.60 kg/m2. [Conclusion] A good habitat had to be created or chosen in the domesticating cultivation of the wild Geg in Ganzi prefecture. The preferred choice was the plastic greenhouse, the alternative one was the deciduous broadleaved forest of shady slope. The imitating wild planting of the wild Geg should be done in the warm moist deciduous broad-leaved forest of shady slope.展开更多
Gastrodia elata Bl.is a traditional Chinese herbal medicine with special pharmacological effects on headache and numbness.However,its wild sources are now in short supply because of over-excavation.A total of 14 endop...Gastrodia elata Bl.is a traditional Chinese herbal medicine with special pharmacological effects on headache and numbness.However,its wild sources are now in short supply because of over-excavation.A total of 14 endophytic fungi were isolated from Gastrodia elata Bl collected from Yinjing,in which 10 strains were identified,including Neonectria,Ascomycota,Fusarium,Oidiodendron and Dothiorella.All crude extracts showed some degrees of antioxidant activities by T-AOC method and DPPH methods,especially those from Oidiodendron GeR6 and Ascomycota sp.GeR2,which might be attributed due to their high phenolic contents.However,only Oidiodendron GeR6 contained gastrodin(39.2μg/g)as the host plant in the crude extracts.In conclusion,the notable activities of GeR6 indicated its medical use as a natural antioxidant agent,as well as substitutable use for Gastrodia elata Bl.in the future.展开更多
基金The National Outstanding Youth Foundation by NSF of ChinaTrans-Century Training Program Foundation for Talents by the Ministry of Education for financial support.
文摘Microbial transformation of gastrodin by Mucor spinosus strain 3.3450, resulted in a product with a transformation rate close to 100 per cent. This product was identified as p-hydroxy benzyl alcohol on the basis of its 1H, 13C NMR and EI-MS spectral data. It could be inferred that the enzyme responsible for the biotransforma-tion reaction was a kind of extracellular and constitutive enzyme since the transformation reaction of the substrate could be carried out in cell free extracts of the fermentation broth of the Mucor spinosus.
基金Supported by Key Project of Natural Sciences of Sichuan Provincial Department of Education(12ZA103)
文摘The effects of the tree species and the dosages of Armillariella mellea rhizomoph wood on asexual propagation of wild Gastrodia elata Bl. f. glauca S. Chow were studied in Ganzi prefecture. The results showed that tree species had a very significant effect on asexual reproduction yield of wild Gastrodia elata Bl. f. glauca S. Chow. The drying rate and yield of Gastrodia elata Bl. f. glauca S. Chow cultured by close grained woods such as Quercus spinosa David ex Fr. was extremely significant higher than that cultured by coarse grained wood such as Betula platyphylla Suk. The dosage had a very significant effect on the yield of wild Gastrodia elata Bl. f. glauca S. Chow whereas it had no influence on the drying rate.
基金Supported by Key Natural Science Program of the Education Department of Sichuan province(12ZA103)
文摘[Objective] The influences of different habitats on asexual propagation of wild Geg(abbreviation for Gastrodia elata Bl. f. glauca S. Chow) being domesticated in Ganzi prefecture was studied. [Method] The research trial was carried out in the following three kinds of habitats at the same time: the plastic greenhouses, the birch forest of shady slope and the shrubbery of sunny slope, and the results were analyzed with group data average hypothesis test method. [Result] The overall tuber(the juvenile tuber, the immature tuber and the mature tuber) yield and the mature tuber yield were both very significantly higher in the plastic greenhouses than in the other two types of habitats, and respectively reached 9.52 and 6.70 kg/m2; the mature tuber drying rate was dramatically or very dramatically lower in the plastic greenhouses than in the other two types of habitats, and was 23.84%; the stabilities of the overall tuber yield, the mature tuber yield and the drying rate were all reduced in the order of greenhouses, birch forest and shrubbery; and the dry mature tuber yield of the plastic greenhouses was the highest in the three kinds of habitats, and was 1.60 kg/m2. [Conclusion] A good habitat had to be created or chosen in the domesticating cultivation of the wild Geg in Ganzi prefecture. The preferred choice was the plastic greenhouse, the alternative one was the deciduous broadleaved forest of shady slope. The imitating wild planting of the wild Geg should be done in the warm moist deciduous broad-leaved forest of shady slope.
基金The Foundation of Industrialization Demonstration and Promotion of Standardized Planting of Dominant Species of Gastrodia elata in Yingjing(General Service Station)the Foundation of Sichuan Sci ence and Technology Plan Project(Grant No.2019YFS0170).
文摘Gastrodia elata Bl.is a traditional Chinese herbal medicine with special pharmacological effects on headache and numbness.However,its wild sources are now in short supply because of over-excavation.A total of 14 endophytic fungi were isolated from Gastrodia elata Bl collected from Yinjing,in which 10 strains were identified,including Neonectria,Ascomycota,Fusarium,Oidiodendron and Dothiorella.All crude extracts showed some degrees of antioxidant activities by T-AOC method and DPPH methods,especially those from Oidiodendron GeR6 and Ascomycota sp.GeR2,which might be attributed due to their high phenolic contents.However,only Oidiodendron GeR6 contained gastrodin(39.2μg/g)as the host plant in the crude extracts.In conclusion,the notable activities of GeR6 indicated its medical use as a natural antioxidant agent,as well as substitutable use for Gastrodia elata Bl.in the future.
