生态环境数据的组织和管理是生态环境信息管理和生态环境保护的基础。3S技术的发展为生态环境数据的信息化提供了强有力的技术支持和解决方案。文章以东江流域为例分析和设计了生态环境数据库的总体架构,根据生态环境数据的特点,采用Arc...生态环境数据的组织和管理是生态环境信息管理和生态环境保护的基础。3S技术的发展为生态环境数据的信息化提供了强有力的技术支持和解决方案。文章以东江流域为例分析和设计了生态环境数据库的总体架构,根据生态环境数据的特点,采用ArcSDE9.3+SQL Server 2005的集成模式构建空间数据库,基于ArcServer REST服务所构建的WebGIS在数据发布方面具有优势。通过提出数据库的"三级结构"概念模型、数据质量控制模型,解决生态环境数据的分布式存储、规范化管理和快速发布等问题,制定出了一套数据库分类与编码、数据库逻辑设计、关系类设计、数据质量控制、数据库存储设计和数据库发布的技术方案,构建出东江流域生态环境数据库与WebGIS,结果证明,此种方法适应于低成本、低硬件配置、开发周期短的数据库构建环境,且运行效率较高。为流域等大尺度区域的生态环境数据库的构建和发布提供参考实例和技术路线。展开更多
Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecula...Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology,quantitative genetics and genomics. Therefore,it is extremely necessary to select several versatile,low-cost,efficient and time-and labor-saving methods to develop a large panel of microsatellite markers. In this study,we used Zhikong scallop(Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency,while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time-and cost-saving,it is difficult to obtain a large number of microsatellite markers,mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study,we recommend two methods,microsatellite-enriched library construction method and FIASCO-colony hybridization method,for large-scale microsatellite marker development. Both methods were derived from the microsatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes.展开更多
The article studies tourism eco-environment of 14 cities of Gangsu Province, China, based on GIS with many kinds of multi-subject spatial database, such as remote sensing data, observation data and literature data. Th...The article studies tourism eco-environment of 14 cities of Gangsu Province, China, based on GIS with many kinds of multi-subject spatial database, such as remote sensing data, observation data and literature data. The research results were as follows. First, spatial features of 14 cities' tourism eco-environment are displayed with five levels of vulnerability respectively. The vulnerability in Gansu becomes worse from Gannan City, located in southern Gansu to Hexi Corridor which lies in northwestern Gansu. Second, the areas of above the middle vulnerability level make up 75% of the total areas of Gansu Province. Third, more than 70% of high-level human and natural tourism resources are in the areas with high vulnerability eco-environment. Fourth, it is crucial to develop comprehensive tourism industry in order to improve the harmonious development between tourism industry and eco-environment in Gansu Province.展开更多
文摘生态环境数据的组织和管理是生态环境信息管理和生态环境保护的基础。3S技术的发展为生态环境数据的信息化提供了强有力的技术支持和解决方案。文章以东江流域为例分析和设计了生态环境数据库的总体架构,根据生态环境数据的特点,采用ArcSDE9.3+SQL Server 2005的集成模式构建空间数据库,基于ArcServer REST服务所构建的WebGIS在数据发布方面具有优势。通过提出数据库的"三级结构"概念模型、数据质量控制模型,解决生态环境数据的分布式存储、规范化管理和快速发布等问题,制定出了一套数据库分类与编码、数据库逻辑设计、关系类设计、数据质量控制、数据库存储设计和数据库发布的技术方案,构建出东江流域生态环境数据库与WebGIS,结果证明,此种方法适应于低成本、低硬件配置、开发周期短的数据库构建环境,且运行效率较高。为流域等大尺度区域的生态环境数据库的构建和发布提供参考实例和技术路线。
基金supported by ‘863’ Program (2006AA10A408 and 2006AA10A411), NSFC30571417, NYHYZX07-047, 2005DKA30470, 2006BAD09A10 and NCET-06-0594.
文摘Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology,quantitative genetics and genomics. Therefore,it is extremely necessary to select several versatile,low-cost,efficient and time-and labor-saving methods to develop a large panel of microsatellite markers. In this study,we used Zhikong scallop(Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency,while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time-and cost-saving,it is difficult to obtain a large number of microsatellite markers,mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study,we recommend two methods,microsatellite-enriched library construction method and FIASCO-colony hybridization method,for large-scale microsatellite marker development. Both methods were derived from the microsatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes.
基金supported by National Natural Science Foundation of China (Grant No.40671062) the Third Knowledge Innovation Project of ‘Study on the Regional Eco-economic Development Theory and Patterns',supported by Institute of Geo-graphical Sciences and Natural Resources Research,CAS
文摘The article studies tourism eco-environment of 14 cities of Gangsu Province, China, based on GIS with many kinds of multi-subject spatial database, such as remote sensing data, observation data and literature data. The research results were as follows. First, spatial features of 14 cities' tourism eco-environment are displayed with five levels of vulnerability respectively. The vulnerability in Gansu becomes worse from Gannan City, located in southern Gansu to Hexi Corridor which lies in northwestern Gansu. Second, the areas of above the middle vulnerability level make up 75% of the total areas of Gansu Province. Third, more than 70% of high-level human and natural tourism resources are in the areas with high vulnerability eco-environment. Fourth, it is crucial to develop comprehensive tourism industry in order to improve the harmonious development between tourism industry and eco-environment in Gansu Province.
