The plant growth and physiological and biochemical responses of root and leaves of grape Yinhong seedlings to the weak lights of 20 000,16 000,12 000,8 000 lx and the normal illumination of 25 000 lx(CK) respectivel...The plant growth and physiological and biochemical responses of root and leaves of grape Yinhong seedlings to the weak lights of 20 000,16 000,12 000,8 000 lx and the normal illumination of 25 000 lx(CK) respectively,were investigated.There was no significant dfference in growth indexes of root and leaves of grapevine seedlings between the light of 20 000 lx and CK,and the light of 16 000 lx and CK for 30 d.The chlorophyll contents,soluble protein contents,net photosynthetic rates,transpiration rates,stomatal conductance,water use efficiency and protective enzyme(CAT,POD,SOD) activities in the leaves under the lights of 20 000 lx and 16 000 lx for 30 d were all higher than those under the lights of 20 000 lx and 16 000 lx for 1 d.Under the light of 8 000 lx for 30 d,the growth indexes of root and leaves of grapevine seedlings were significantly lower than those of CK,and except for MDA content,most physiological and biochemical indexes of the leaves were significantly lower than those under the light of 8 000 lx for 1 d.Under12 000 lx,the values of most growth indexes in root and leaves and physiological and biochemical indexes in leaves were between the 16 000 lx and 8 000 lx.In conclusion,Yinhong could grow under the lights above 16 000 lx,and would be stunted by the weak light below 8 000 lx.展开更多
AIM:To establish an appropriate primate model of fulminant hepatic failure (FHF).METHODS:We have,for the first time,established a large animal model of FHF in Macaca mulatta by intraperitoneal infusion of amatoxin and...AIM:To establish an appropriate primate model of fulminant hepatic failure (FHF).METHODS:We have,for the first time,established a large animal model of FHF in Macaca mulatta by intraperitoneal infusion of amatoxin and endotoxin.Clinical features,biochemical indexes,histopathology and iconography were examined to dynamically investigate the progress and outcome of the animal model.RESULTS:Our results showed that the enzymes and serum bilirubin were markedly increased and the enzyme-bilirubin segregation emerged 36 h after toxin administration.Coagulation activity was significantly decreased.Gradually deteriorated parenchymal abnormality was detected by magnetic resonance imaging (MRI) and ultrasonography at 48 h.The liver biopsy showed marked hepatocyte steatosis and massive parenchymal necrosis at 36 h and 49 h,respectively.The autopsy showed typical yellow atrophy of the liver.Hepatic encephalopathy of the models was also confirmed by hepatic coma,MRI and pathological changes of cerebral edema.The lethal effects of the extrahepatic organ dysfunction were ruled out by their biochemical indices,imaging and histopathology.CONCLUSION:We have established an appropriate large primate model of FHF,which is closely similar to clinic cases,and can be used for investigation of the mechanism of FHF and for evaluation of potential medical therapies.展开更多
Objective: The aim of this study was to identify the correlation between the clinicopathological characteristics and recurrence in early gastric cancer (EGC), what's more, we attempt to look for a predictive bioma...Objective: The aim of this study was to identify the correlation between the clinicopathological characteristics and recurrence in early gastric cancer (EGC), what's more, we attempt to look for a predictive biomarker to predict and treat for re-currence of EGC. Methods: This study retrospectively analyzed 178 early gastric cancer patients who had the complete post-operative and follow-up medical records in the First Affiliated Hospital of Yangtze University (China) between January 1995 to December 2005. All of them were followed-up to December 2009 regularly. Computer tomography (CT), endoscopy, and single photon emission computed tomography (SPET-CT) were used to diagnose for recurrence of EGC. Immunohistochem-istry (IHC) and fluorescence in situ hybridization (FISH) were used for the detection of cerbB2. Chi-square test was applied to this study for statistics analysis. Results: Fourteen patients had recurrence. Eighteen patients were cerbB2-positive, including twelve recurrence patients and six norecurrence patients. Sex, tumor depth, and lymph node metastasis were related to the recurrence of EGC. Also, cerbB2-positive patients had the higher recurrence rate compared to the cerbB2-negative patients. Conclusion: Recurrence of EGC after curative resection can be predicted by using some clinicopathological characteristics. CerbB2 can be used as a predictive biomarker for recurrence of EGC.展开更多
Jilong is a place behind The Himalayas,which is almost isolated from the world.There is typical vertical ecosystem thanks to its special geological environment. So it is honored as “the most abundant species gene war...Jilong is a place behind The Himalayas,which is almost isolated from the world.There is typical vertical ecosystem thanks to its special geological environment. So it is honored as “the most abundant species gene warehouse in the Plateau Area”and “the most beautiful valley in the world”.To exploit this valleyis necessary along with the construction of Jilong Porton the border of Sino-Nepal. Based on the research on the spot,the ecological resources there are evaluated and an idea that regards hiking tourism asmain form and ecotourismas the theme is put forward in this paper,which explores a way to develop the valley on the basis of sustainability.展开更多
Humus forms, especially the occurrence and the thickness of the horizon of humified residues (OH), provide valuable information on site conditions. In mountain forest soils, humus forms show a high spatial variabili...Humus forms, especially the occurrence and the thickness of the horizon of humified residues (OH), provide valuable information on site conditions. In mountain forest soils, humus forms show a high spatial variability and data on their spatial patterns is often scarce. Our aim was to test the applicability of various vegetation features as proxy for OH thickness. Subalpine coniferous forests dominated by Picea abies (L.) H. Karst. and Larix decidua Mill. were studied in the Province of Trento, Italian Alps, between ca. 900 and 22o0 m a.s.1. Braun-Blanquet vegetation relevds and OH thickness were recorded at 152 plots. The vegetation parameters, tested for their suitability as indicators of OH thickness, encompassed mean Landolt indicator values of the herb layer (both unweighted and cover-weighted means) as well as parameters of vegetation structure (cover values of plant species groups) calculated from the releves. To our knowledge, the predictive power of Landolt indicator values (LIVs) for humus forms had not been tested before. Correlations between OHthickness and mean LIVs were strongest for the soil reaction value, but indicator values for humus, nutrients, temperature and light were also significantly correlated with OH thickness. Generally, weighting with species cover reduced the indicator quality of mean LIVs for OH thickness. The strongest relationships between OH thickness and vegetation structure existed in the following indicators: the cover of forbs (excluding graminoids and ferns) and the cover of Erieaeeae in the herb layer. Regression models predicting OH thickness based on vegetation structure had almost as much predictive power as models based on LIVs. We conclude that LIVs analysis can produce fairly reliable information regarding the thickness of the OH horizon and, thus, the humus form. If no releve data are readily available, a field estimation of the cover values of certain easily distinguishable herb layer species groups is much faster than a vegetation survey with consecutive indicator value analysis, and might be a feasible way of quickly indicating the humus form.展开更多
Objective:The aim of this study was to explore the expression of manganese superoxide dismutase(MnSOD) in esophageal squamous cell carcinoma and its relationship with clinicopathological characteristics and its biolog...Objective:The aim of this study was to explore the expression of manganese superoxide dismutase(MnSOD) in esophageal squamous cell carcinoma and its relationship with clinicopathological characteristics and its biological behavior.Methods:Immunohistochemical method(SP method),reverse transcription-polymerase chain reaction(RT-PCR) and Western blot were combined to detect the MnSOD protein and mRNA expression in 45 cases of esophageal squamous cell carcinoma and the normal tissue that was 5 cm apart from the edge of esophageal cancer lesion and without documented microscopic invasive cancer.Meanwhile,analysis was performed on the relationship between the pathological features of esophageal cancer and its biological behavior.Results:In esophageal squamous cell carcinoma and normal esophageal tissue,MnSOD protein expression was identified in 31.1%(14/45) and 86.7%(31/45)(P = 0.003),respectively,with the relative expression levels of MnSOD mRNA were 0.310 ± 0.036 and 0.482 ± 0.053(P = 0.000).The longer the lesions and the deeper the invasion,the differentiation would become poorer and the expression level of MnSOD would get lower,indicating that the level of MnSOD protein and mRNA expression were closely related to esophageal squamous cell carcinoma in the length of lesion,depth of invasion,and degree of differentiation(P < 0.05).Nevertheless,it showed no association with the presence of the lymph node metastasis,lesion site and the macroscopic classification(P > 0.05).Conclusion:The MnSOD protein and mRNA expression were both decreased in esophageal squamous cell carcinoma tissue.This may be related to the carcinogenesis and development of esophageal cancer.Detection of the expression of MnSOD would be of clinical significance in understanding the prognosis and guiding therapeutic strategy of esophageal cancer.展开更多
基金Supported by Science and Technology Innovation Team Project of Ningbo Province of China(2011B82019)Supported by Natural Science Foundation of Ningbo Province of China(Y13C150014)+1 种基金Supported by Foundation of Zhejiang Educational Committee(2011C32008)Supported by Science and Technology Project of Ningbo Province,China(2010C91049)~~
文摘The plant growth and physiological and biochemical responses of root and leaves of grape Yinhong seedlings to the weak lights of 20 000,16 000,12 000,8 000 lx and the normal illumination of 25 000 lx(CK) respectively,were investigated.There was no significant dfference in growth indexes of root and leaves of grapevine seedlings between the light of 20 000 lx and CK,and the light of 16 000 lx and CK for 30 d.The chlorophyll contents,soluble protein contents,net photosynthetic rates,transpiration rates,stomatal conductance,water use efficiency and protective enzyme(CAT,POD,SOD) activities in the leaves under the lights of 20 000 lx and 16 000 lx for 30 d were all higher than those under the lights of 20 000 lx and 16 000 lx for 1 d.Under the light of 8 000 lx for 30 d,the growth indexes of root and leaves of grapevine seedlings were significantly lower than those of CK,and except for MDA content,most physiological and biochemical indexes of the leaves were significantly lower than those under the light of 8 000 lx for 1 d.Under12 000 lx,the values of most growth indexes in root and leaves and physiological and biochemical indexes in leaves were between the 16 000 lx and 8 000 lx.In conclusion,Yinhong could grow under the lights above 16 000 lx,and would be stunted by the weak light below 8 000 lx.
基金Supported by National Basic Research Program of China,No.2009CB522401grand from Natural Science Foundation ofChina,No. 30870983 and 30971118
文摘AIM:To establish an appropriate primate model of fulminant hepatic failure (FHF).METHODS:We have,for the first time,established a large animal model of FHF in Macaca mulatta by intraperitoneal infusion of amatoxin and endotoxin.Clinical features,biochemical indexes,histopathology and iconography were examined to dynamically investigate the progress and outcome of the animal model.RESULTS:Our results showed that the enzymes and serum bilirubin were markedly increased and the enzyme-bilirubin segregation emerged 36 h after toxin administration.Coagulation activity was significantly decreased.Gradually deteriorated parenchymal abnormality was detected by magnetic resonance imaging (MRI) and ultrasonography at 48 h.The liver biopsy showed marked hepatocyte steatosis and massive parenchymal necrosis at 36 h and 49 h,respectively.The autopsy showed typical yellow atrophy of the liver.Hepatic encephalopathy of the models was also confirmed by hepatic coma,MRI and pathological changes of cerebral edema.The lethal effects of the extrahepatic organ dysfunction were ruled out by their biochemical indices,imaging and histopathology.CONCLUSION:We have established an appropriate large primate model of FHF,which is closely similar to clinic cases,and can be used for investigation of the mechanism of FHF and for evaluation of potential medical therapies.
文摘Objective: The aim of this study was to identify the correlation between the clinicopathological characteristics and recurrence in early gastric cancer (EGC), what's more, we attempt to look for a predictive biomarker to predict and treat for re-currence of EGC. Methods: This study retrospectively analyzed 178 early gastric cancer patients who had the complete post-operative and follow-up medical records in the First Affiliated Hospital of Yangtze University (China) between January 1995 to December 2005. All of them were followed-up to December 2009 regularly. Computer tomography (CT), endoscopy, and single photon emission computed tomography (SPET-CT) were used to diagnose for recurrence of EGC. Immunohistochem-istry (IHC) and fluorescence in situ hybridization (FISH) were used for the detection of cerbB2. Chi-square test was applied to this study for statistics analysis. Results: Fourteen patients had recurrence. Eighteen patients were cerbB2-positive, including twelve recurrence patients and six norecurrence patients. Sex, tumor depth, and lymph node metastasis were related to the recurrence of EGC. Also, cerbB2-positive patients had the higher recurrence rate compared to the cerbB2-negative patients. Conclusion: Recurrence of EGC after curative resection can be predicted by using some clinicopathological characteristics. CerbB2 can be used as a predictive biomarker for recurrence of EGC.
文摘Jilong is a place behind The Himalayas,which is almost isolated from the world.There is typical vertical ecosystem thanks to its special geological environment. So it is honored as “the most abundant species gene warehouse in the Plateau Area”and “the most beautiful valley in the world”.To exploit this valleyis necessary along with the construction of Jilong Porton the border of Sino-Nepal. Based on the research on the spot,the ecological resources there are evaluated and an idea that regards hiking tourism asmain form and ecotourismas the theme is put forward in this paper,which explores a way to develop the valley on the basis of sustainability.
基金funded by the German Research Foundation(DFG)(Grant No.Br1106/23-1)the Swiss National Science Foundation(SNF)(Grant No.205321L_141186)the Austrian Science Fund(FWF)
文摘Humus forms, especially the occurrence and the thickness of the horizon of humified residues (OH), provide valuable information on site conditions. In mountain forest soils, humus forms show a high spatial variability and data on their spatial patterns is often scarce. Our aim was to test the applicability of various vegetation features as proxy for OH thickness. Subalpine coniferous forests dominated by Picea abies (L.) H. Karst. and Larix decidua Mill. were studied in the Province of Trento, Italian Alps, between ca. 900 and 22o0 m a.s.1. Braun-Blanquet vegetation relevds and OH thickness were recorded at 152 plots. The vegetation parameters, tested for their suitability as indicators of OH thickness, encompassed mean Landolt indicator values of the herb layer (both unweighted and cover-weighted means) as well as parameters of vegetation structure (cover values of plant species groups) calculated from the releves. To our knowledge, the predictive power of Landolt indicator values (LIVs) for humus forms had not been tested before. Correlations between OHthickness and mean LIVs were strongest for the soil reaction value, but indicator values for humus, nutrients, temperature and light were also significantly correlated with OH thickness. Generally, weighting with species cover reduced the indicator quality of mean LIVs for OH thickness. The strongest relationships between OH thickness and vegetation structure existed in the following indicators: the cover of forbs (excluding graminoids and ferns) and the cover of Erieaeeae in the herb layer. Regression models predicting OH thickness based on vegetation structure had almost as much predictive power as models based on LIVs. We conclude that LIVs analysis can produce fairly reliable information regarding the thickness of the OH horizon and, thus, the humus form. If no releve data are readily available, a field estimation of the cover values of certain easily distinguishable herb layer species groups is much faster than a vegetation survey with consecutive indicator value analysis, and might be a feasible way of quickly indicating the humus form.
基金Supported by a grant from the National Natural Science Foundation of China (No. 30540005)
文摘Objective:The aim of this study was to explore the expression of manganese superoxide dismutase(MnSOD) in esophageal squamous cell carcinoma and its relationship with clinicopathological characteristics and its biological behavior.Methods:Immunohistochemical method(SP method),reverse transcription-polymerase chain reaction(RT-PCR) and Western blot were combined to detect the MnSOD protein and mRNA expression in 45 cases of esophageal squamous cell carcinoma and the normal tissue that was 5 cm apart from the edge of esophageal cancer lesion and without documented microscopic invasive cancer.Meanwhile,analysis was performed on the relationship between the pathological features of esophageal cancer and its biological behavior.Results:In esophageal squamous cell carcinoma and normal esophageal tissue,MnSOD protein expression was identified in 31.1%(14/45) and 86.7%(31/45)(P = 0.003),respectively,with the relative expression levels of MnSOD mRNA were 0.310 ± 0.036 and 0.482 ± 0.053(P = 0.000).The longer the lesions and the deeper the invasion,the differentiation would become poorer and the expression level of MnSOD would get lower,indicating that the level of MnSOD protein and mRNA expression were closely related to esophageal squamous cell carcinoma in the length of lesion,depth of invasion,and degree of differentiation(P < 0.05).Nevertheless,it showed no association with the presence of the lymph node metastasis,lesion site and the macroscopic classification(P > 0.05).Conclusion:The MnSOD protein and mRNA expression were both decreased in esophageal squamous cell carcinoma tissue.This may be related to the carcinogenesis and development of esophageal cancer.Detection of the expression of MnSOD would be of clinical significance in understanding the prognosis and guiding therapeutic strategy of esophageal cancer.