[Objective] The aim was to optimized and established the regeneration system of Moringa oleifera and provided foundation for its rapid propagation and further research. [Method] Tissue culture techniques and large sca...[Objective] The aim was to optimized and established the regeneration system of Moringa oleifera and provided foundation for its rapid propagation and further research. [Method] Tissue culture techniques and large scale micropropagation of M. oleifera were studied in this paper. By means of a series of experiments, we used the leaf of aseptic seedling from M. oleifera as explants to optimize and establish the regeneration system cultured in vitro by means of direct organogenesis. [Result] It was observed that using the fresh shelled M. oleifera seeds with 0.1% mercuric chloride for 6 minutes could reach the best disinfection effect. The seed germination rate was 85%. The leaf could produce cluster buds well using the medium with MS＋2.0 mg/L 6-BA＋0.05 mg/L NAA, while the best proliferation condition was under MS＋6-BA 1.0 mg/L＋KT 0.1 rag/L＋2, 4-D 2.0 mg/L＋NAA 0.05 mg/L. The best rooting induction culture medium was MS＋0.5 mg/L IBA, with the rooting rate as 100%. [Cenclusien] This protocol might find use in mass production of true- to-type plants and in production of transgenic plants through Agrobacterium/biolisticmediated transformation.展开更多
The root morphology of erect type peanut in deep soil was studied in this paper. In the experiment, erect type peanut showed as most as five-order lateral roots with 13 227 pieces of lateral roots. At the seedling sta...The root morphology of erect type peanut in deep soil was studied in this paper. In the experiment, erect type peanut showed as most as five-order lateral roots with 13 227 pieces of lateral roots. At the seedling stage, the root system of erect type peanut was handstand cone-typed with lateral roots at various orders distributed around the taproot, and among the roots, the taproot was longest. During the late seedling stage to mature stage, the upper part of the root system of erect type peanut was blunt cone-typed, the middle part was three-dimensional network typed, while the lower part was cyUnder-like. The longest first-order lateral root was the longest root. At the mature stage, the taproot was not always the deepest root.展开更多
基金National Natural Science Foundation of China(21165008)~~
文摘[Objective] The aim was to optimized and established the regeneration system of Moringa oleifera and provided foundation for its rapid propagation and further research. [Method] Tissue culture techniques and large scale micropropagation of M. oleifera were studied in this paper. By means of a series of experiments, we used the leaf of aseptic seedling from M. oleifera as explants to optimize and establish the regeneration system cultured in vitro by means of direct organogenesis. [Result] It was observed that using the fresh shelled M. oleifera seeds with 0.1% mercuric chloride for 6 minutes could reach the best disinfection effect. The seed germination rate was 85%. The leaf could produce cluster buds well using the medium with MS＋2.0 mg/L 6-BA＋0.05 mg/L NAA, while the best proliferation condition was under MS＋6-BA 1.0 mg/L＋KT 0.1 rag/L＋2, 4-D 2.0 mg/L＋NAA 0.05 mg/L. The best rooting induction culture medium was MS＋0.5 mg/L IBA, with the rooting rate as 100%. [Cenclusien] This protocol might find use in mass production of true- to-type plants and in production of transgenic plants through Agrobacterium/biolisticmediated transformation.
文摘The root morphology of erect type peanut in deep soil was studied in this paper. In the experiment, erect type peanut showed as most as five-order lateral roots with 13 227 pieces of lateral roots. At the seedling stage, the root system of erect type peanut was handstand cone-typed with lateral roots at various orders distributed around the taproot, and among the roots, the taproot was longest. During the late seedling stage to mature stage, the upper part of the root system of erect type peanut was blunt cone-typed, the middle part was three-dimensional network typed, while the lower part was cyUnder-like. The longest first-order lateral root was the longest root. At the mature stage, the taproot was not always the deepest root.
