斜带石斑鱼雌鱼卵巢发育与血清性类固醇激素的生殖周期变化

斜带石斑鱼Epinepheluscoioides卵母细胞发育可分为6个时相,以卵母细胞在卵巢中组成的差异可以把卵巢发育划分为相应6个时期。测定了成熟雌鱼血清中雌二醇和睾酮含量的生殖周期变化,在生殖季节成熟雌鱼血清中雌二醇和睾酮含量最高。生殖季节成熟雌鱼的平均性腺成熟系数GSI显著高于其它月份,并且血清性类固醇激素水平的季节变化与性腺成熟系数的变化基本一致,都是在生殖季节达到最高峰。

长臀脑垂体和血清中促性腺激素的生殖周期变化

Changes in the pituitary and serum gonadotropin levels of Cranoglanis bouderius were studied at different stages of ovarian development. Based on African catfish gonadotropin (cfGTH) and its anti-serum (Racf), heterologous radio-immunoassay (RIA) for the gonadotropin (GTH) of Cranoglanis bouderius was developed. The pituitary GTH contents at the late development stage were significantly higher than that at the mature, ovulation or regressed stages, while serum GTH levels at maturation and ovulation were significantly higher than that at late development and regressed stages. Injection of gonadotropin-releasing hormone analogue (GnRH-A) stimulated GTH secretion. Domperidone (DOM) had no effect on the basal serum GTH level. Injection of GnRH-A and DOM significantly enhanced the GTH levels stimulated by GnRH-A alone. These results indicate that GTH release in Cranoglanis bouderius is under the dual regulation of gonadotropin releasing hormone (GnRH) and dopamine (DA) from the hypothalamus, but DA can only inhibit GTH release induced by GnRH and not basal GTH release.

围绝经期卵巢血流动力的彩色多普勒超声研究

目的:研究围绝经期卵巢的血流信号及频谱规律。方法:无盆腔疾患45～55岁围绝经期妇女90名,分三组,观察血流形态,测量平均彩色象素密度(MCD),收缩期峰值流速(PS),阻力指数(RI)并进行统计学处理。结果:(1)卵巢门及内部血流PS值较围绝经前期减低(P<0.05)。RI值增高(P<0.01)。 (2)卵巢的MCD与PS及RI均无相关性(P>0.05)。结论:围绝经期卵巢的血流参数,血管形态与围绝经前期均有不同程度的改变。