期刊文献+
共找到5篇文章
< 1 >
每页显示 20 50 100
Association Between Genital Ulcer Disease and HIV Seroprevalence among STD Patients in Guangzhou
1
作者 朱慧兰 苏向阳 +5 位作者 韩尔阳 程文海 武明昌 林霭 丘小珊 张锡宝 《Chinese Journal of Sexually Transmitted Infections》 2004年第1期32-35,64,共5页
Objectives: To understand genital ulcer disease(GUD) among patients attending sexually transmitteddisease (STD) clinics in Guangzhou, China, and itsassociation with HIV infection.Methods: Between September 9th, 1997 a... Objectives: To understand genital ulcer disease(GUD) among patients attending sexually transmitteddisease (STD) clinics in Guangzhou, China, and itsassociation with HIV infection.Methods: Between September 9th, 1997 and Octo-ber 30th, 2002, 8 962 patients with STDs wereevaluated. 285 patients were diagnosed with GUD basedon clinical manifestations and microbiologic evalua-tions including dark field microscopy and serologytest for syphilis (RPR, TPPA). Swabs of each genitalulcer were processed in a multiplex PCR assay (M-PCR) for simultaneous detection of Herpes simplexvirus (HSV), Treponema pallium, and Haemophilusducreyi. Other STDs were classified by routine diag-nostic criteria, including microscopy or culture forNeisseria gonorrhoeae, Chlamydia trachomatis,Urea- plasma urealyticum, Human papillomavirus,Trichomonas, etc.Results: Of the 8 962 patients with STDs, the HIVseroprevalence in patients with and without GUD was1.75% (5/285) and 1.53% (133/8677), respectively,with no statistically significant difference (χ 2=0.09,P>0.05; OR=1.15, 95%CI=0.47-2.81) . HIVseroprevalence in patients with syphilis, genital her-pes and other STDs was 2.81% (22/784), 0.74% (6/814) and 1.49% (110/7 364), respectively. Prevalencein patients with syphilis was significantly higher thanthat in patients with genital herpes and other STDs,(χ 2=9.92, P<0.005, OR=3.89, 95%CI=1.67-9.05;χ 2=7.66, P<0.001, OR=1.90, 95%CI=1.21-3.00).Conclusions: The study shows that the HIV sero-prevalence in this population of patients with GUDis very low. The results also indicate an associationbetween syphilis and HIV infection. The relationshipbetween genital herpes and HIV infection needsfurther research. 展开更多
关键词 genital ulcer sexually transmitteddisease HIV
下载PDF
信息
2
《四川省卫生管理干部学院学报》 2008年第3期173-173,175+183+207+217+224,共6页
关键词 生殖器溃疡病 依法利珠单抗 线粒体肌病 传染力 芳香酶抑制剂 髋关节成形术 利伐沙班 包皮环切术 切除术 银屑病关节炎 信息传递 情报传递 依诺肝素 研究者 西洛他唑 HIV 子宫内膜异位症 子宫内膜异位 贴剂
下载PDF
DETECTION OF PATHOGENS CAUSING GENITAL ULCER DISEASE BY MULTIPLEX POLYMERASE CHAIN REACTION 被引量:3
3
作者 Ai-ying Liu Ming-jun Jiang +1 位作者 Yue-ping Yin Jiang-fang Sun 《Chinese Medical Sciences Journal》 CAS CSCD 2005年第4期273-275, ,共3页
Objective To establish a multiplex polymerase chain reaction (M-PCR) assay for simultaneous detection of pathogens causing genital ulcer disease (GUD). Mothods Based on the gene-specific region of the following p... Objective To establish a multiplex polymerase chain reaction (M-PCR) assay for simultaneous detection of pathogens causing genital ulcer disease (GUD). Mothods Based on the gene-specific region of the following pathogens: Chlamydia trachomatis omp l/ompb, herpes simplex virus (HSV) DNA polymerase, Treponema pollidum tpp47, Haemophilus ducreyi 16s rRNA, four sets of primers were designed and an M-PCR assay was developed to detect four pathogens in one test. The assay was evaluated with diagnostic result of golden standard for each pathogen.Results Of the 51 clinical samples, M-PCR showed slightly higher positive rate (47.1%) of HSV than cell culture (23.6%). Meanwhile, the positive rate of T. pallidum detected by M-PCR and dark-field microscopy was 19.6% (10/51) and 15.7% (8/51), respectively. Only one sample was positive for H. ducreyi and no sample was positive for C. trachomatis detected by both M-PCR assay and culture. Conclusion This primary study indicated that M-PCR assay can simultaneously and rapidly detect the four etiologic pathogens causing GUD. 展开更多
关键词 multiplex polymerase chain reaction genital ulcer disease
下载PDF
Facilitating the Implementation of Sexually Transmitted Infection Syndromic Management in China
4
作者 王千秋 邵长庚 《Chinese Journal of Sexually Transmitted Infections》 2002年第4期1-6,共6页
Objerctive: To introduce the current status of implementing sexually transmitted infection (STIs)syndromic management in China. Methods: Data werecollected and analyzed from the literature. Possiblesolutions were sugg... Objerctive: To introduce the current status of implementing sexually transmitted infection (STIs)syndromic management in China. Methods: Data werecollected and analyzed from the literature. Possiblesolutions were suggested for the obstacles encountered inthe implementation process. Results: Validation of revised flowcharts for themanagement of three syndromes (urethritis, vaginaldischarge, and genital ulcers) has been conducted in China.The feasibility, effectiveness and acceptance of using asyndromic STI approach were investigated in a large studyin Hainan, Zhejiang and Hubei Provinces. Chief obstaclesto implementation included the following: lack of supportfrom government authorities; negative reaction by hospitals;lack of critical thinking and innovation; perceivedover-emphasis on treatment at the expense of prevention;and lack of coverage for several important pathogens withintreatment algorithms. Possible solutions may include policyadvocacy, adopting syndromic management into a Chinesecontext, ssemination and promotion, training of providers, encouraging use of syndromic approach, and conductingoperational rescarch. Conclusions: Syndromic STI management provides afeasible approach for the diagnosis and treatment of STIs inChina. Further efforts should be made for scaling up itsapplication in clinical settings. 展开更多
关键词 sexually transmitted infections syndromic approach
下载PDF
Detection of Treponema pallidum,Herpes Simplex Virus,and Haemophilus ducreyi from Genital Ulcers by Multiples Polymerase Chain Reaction
5
作者 周华 傅笑冰 +4 位作者 熊礼宽 杨帆 洪福昌 曾序春 董时富 《Chinese Journal of Sexually Transmitted Infections》 2001年第1期34-39,共6页
Objective: To evaluate the clinical application of multiplex PCR inthe detection of Treponema pallidum, Herpes simplex virus (HSV), andHaemophilus ducreyi. Method: Three standard strains were used to set up a multiple... Objective: To evaluate the clinical application of multiplex PCR inthe detection of Treponema pallidum, Herpes simplex virus (HSV), andHaemophilus ducreyi. Method: Three standard strains were used to set up a multiplexPCR (MPCR) for detecting syphilis, herpes genitalis, and chancroidsimultaneously. Samples from 122 patients with genital ulcer disease(GUD) were subjected to MPCR and the results were compared withthose of dark-field microscopy and TP serology, HSV antigen ELISA,and H. ducreyi culture. Results: In the 122 patients with GUD, MPCR identified 34 casesof T. pallidum infection, 40 cases of HSV infection, and 2 cases of mixedinfection of T. pollidum and herpes. No positive results of H. ducreyiwere found. The sensitivity of MPCR to T. pallidum and herpes was100% and 93.3%, respectively. The sensitivities of dark-field mi-croscopy and TP serology, HSV antigen ELISA, and H. ducreyi cul-ture was 35.3%, 50% and 100%, respectively. Conclusion: MPCR showed a relatively higher sensitivity for T.pallidum as compared with the routine techniques. Although its sensi-tivity for HSV was not as good as that of antigen ELISA, it also yieldeda high detection rate. MPCR can detect more than one pathogen. It issimple, quick, sensitive, and suitable for clinical use or epidemiologicalinvestigation. 展开更多
关键词 Multiplex PCR Treponema pallidum Herpes Simplex Virus Haemophilus ducreyi
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部