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双酚A生殖毒性及胚胎毒性的研究进展 被引量:3
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作者 何东杰 《河南中医》 2010年第6期624-625,共2页
通过对双酚A的生殖毒性,包括雄性生殖毒性、双酚A雄性生殖毒性机制和雌性生殖毒性;对双酚A的胚胎毒性的研究,涉及双酚A对雌鼠的胚胎毒性和双酚A对子一代雄鼠的遗传毒性;为临床建立有效的预防及治疗体系将具有极其重要意义。
关键词 双酶A 生殖毒性 生殖毒性机制 胚胎毒性
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雷公藤内酯醇对雄性大鼠生殖毒性的机制研究(Ⅱ) 被引量:5
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作者 黄郑隽 彭华毅 +3 位作者 阙慧卿 林绥 郭舜民 钱丽萍 《中国医院药学杂志》 CAS 北大核心 2019年第6期566-569,共4页
目的:考察雷公藤内酯醇对大鼠睾丸细胞相关凋亡基因mRNA表达的影响,研究雷公藤内酯醇生殖毒性的分子机制。方法:以低(0.025 mg·kg^(-1))、中(0.05 mg·kg^(-1))、高(0.1 mg·kg^(-1))剂量的雷公藤内酯醇对健康雄性Wistar... 目的:考察雷公藤内酯醇对大鼠睾丸细胞相关凋亡基因mRNA表达的影响,研究雷公藤内酯醇生殖毒性的分子机制。方法:以低(0.025 mg·kg^(-1))、中(0.05 mg·kg^(-1))、高(0.1 mg·kg^(-1))剂量的雷公藤内酯醇对健康雄性Wistar大鼠连续灌胃染毒30 d,每天一次,于末次染毒24 h后处死大鼠,取睾丸组织进行病理学检查,并通过实时荧光定量PCR测定Bcl-2、Bax、Fas、FasL、CREM和Caspase-3基因mRNA的表达情况。结果:与阴性对照组相比,雷公藤内酯醇染毒组睾丸组织生精细胞明显减少,精索内几乎无精子;高剂量组Bcl-2、CREM mRNA表达降低;而Bax mRNA表达水平在中、高剂量组时呈显著地高表达;Fas和FasL mRNA表达水平在高剂量组显著上升;Caspase-3 mRNA表达水平呈现依赖剂量的高表达,中、高剂量时呈现显著性差异。结论:提示在本实验染毒剂量范围内,特别是高剂量的雷公藤内酯醇能够使生殖细胞相关凋亡基因Bcl-2、Bax、Fas、FasL、CREM和Caspase-3不同程度的表达异常,这很可能是雷公藤内酯醇诱导大鼠生殖细胞凋亡的重要原因,为进一步深入阐述雷公藤内酯醇雄性生殖毒性的分子机制提供了依据。 展开更多
关键词 雷公藤内酯醇 睾丸细胞 生殖毒性机制 凋亡基因
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Pollution of Environmental Endocrine Disrupting Chemicals(EDCs) in Water and Its Adverse Reproductive Effect on Fish 被引量:3
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作者 孟顺龙 宋超 +3 位作者 范立民 裘丽萍 陈家长 徐跑 《Agricultural Science & Technology》 CAS 2014年第3期463-469,共7页
Environmental endocrine disrupting chemicals (EDCs), commonly found in the environment, come from industry and agriculture, including pesticides, fungicides, insecticides, herbicides, and other chemicals. Nowadays, ... Environmental endocrine disrupting chemicals (EDCs), commonly found in the environment, come from industry and agriculture, including pesticides, fungicides, insecticides, herbicides, and other chemicals. Nowadays, more and more EDCs were released into the environment. EDCs go into water body via atmosphere sedi-mentation, surface runoff, soil eluviation, etc., so water body becomes the main place for existing. In order to attract scientific and public attention worldwide and to prevent EDCs pol ution, in this study we reviewed the classification of EDCs and their concentrations in natural water bodies, drinking water sources and water plants, and the reproductive toxicity of EDCs to fish were reviewed. EDCs could disturb the endocrine system and make reproductive organs and reproduction abnor-mal, resulting in fertility descending, reproduction function damage, community quan-tity decrease and even species extinction. In addition, EDCs could disrupt the homeostasis maintained by hormones, which would result in defects of neural de-velopment and abnormalities of the endocrine and reproductive systems. The exact molecular mechanisms have not been completely reported, but researches have suggested that multiple mechanisms were involved in the action of EDCs. Although there have been researches on the biohazard of EDCs, there stil exist problems of weakness in fundamental researches, difficulties in recognizing and identifying EDCs and high cost, which restraint the knowledge on them. 展开更多
关键词 Environmental endocrine disrupting chemical Water body Pollution situ-ation FISH Damage of reproduction
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