[Objective]The aim was to commercialize micropropagation of pistachio and provide good foundations for varieties improvement. [Method]The seeds and stem-segments were used as explants in the tissue culture of Pistacia...[Objective]The aim was to commercialize micropropagation of pistachio and provide good foundations for varieties improvement. [Method]The seeds and stem-segments were used as explants in the tissue culture of Pistacia vera ＇Kerman＇,tissue culture condition and medium and transplant matrix were researched for micropropagation of pistachio,[Result]Mediums of 1/2DKW and 1/2DKW＋3.00 mg/L of 6-BA ＋0.05 mg/L of NAA were suitable medium for seed germination and axillary bud induction of pistachio; Proliferation coefficient was 3.6 on the medium of 1/2DKW ＋4.00 mg/L of 6-BA ＋ 0.05 mg/L of IBA; Rooting rate was up to 75% on the medium of 1/2DKW ＋ 5.00 mg/L of IBA ＋ 1.00 mg/L of NAA; The suitable transplantation matrix was 3/4 sand ＋ 1/4 vermiculite,and survival rate of plant with root was above 79%. [Conclusion]The pistachio （Kerman） tissue culture technology system was established.展开更多
The research explored rapid multiplication on Bletilla striata and the re- suits showed that the medium of MS＋2.5 mg/L6-BA＋0.2 mg/L NAA performed the best in induction, with induction rate at 98%; the medium with MS...The research explored rapid multiplication on Bletilla striata and the re- suits showed that the medium of MS＋2.5 mg/L6-BA＋0.2 mg/L NAA performed the best in induction, with induction rate at 98%; the medium with MS＋IAA2.5mg/L＋ 2.5 mg/L6-BA＋2.0 mg/L NAA was the best in terms of induction and multiplication; the medium with 1/2MS＋0.01 mg/L NAA 1＋0.01 mg/L IAA promoted rooting.The harden- ing temperature was the optimal at 23℃-28 ℃ and relative humidity of 80%-90% the best.展开更多
[Objective] This study was conducted to acquire a large quantity of wide raspberry plantlets and provide a basis for large-scale production. [Method] Re- search was performed on series tissue culture propagation of wi...[Objective] This study was conducted to acquire a large quantity of wide raspberry plantlets and provide a basis for large-scale production. [Method] Re- search was performed on series tissue culture propagation of wide raspberry. [Re- sult] Adventitious shoots were induced with stem segments, and the best induction medium was MS ＋1.5 mg/L BA ＋0.3 mg/L NAA with an induction rate of 96%; and the best differentiation medium was MS ＋2.0 mg/L KT ＋0.5 mg/L NAA, which ex- hibited high root number and quality, with an average root number of 4.5 per plant. The substratum for training plantlets was leaf mould 60%＋perlite 30%＋vermiculite 10%; and the temperature of 23-27℃ and relative air humidity of 80%-90% were beneficial to survival of trained plantlets.[Conclusion] This study laid a foundation for further large-area cultivation of Rubus chingii Hu.展开更多
Black rockfish(Sebastes schlegeli) is an important species for culture; however, its reproductive characteristics have not been fully documented. In this study, we investigated the morphology and developmental process...Black rockfish(Sebastes schlegeli) is an important species for culture; however, its reproductive characteristics have not been fully documented. In this study, we investigated the morphology and developmental process of germ cells in this ovoviviparous rockfish in reproductive season(October 2011–November 2012) with histological methods. We found that the gonad of mature fish showed notable seasonal changes in developmental characteristics and morphological structure. The sperm cells matured during a period lasting from October to December, significantly earlier than the oocytes did. A large number of spermatozoa and other cells occurred in testis at different developmental stages. Vitellogenesis in oocytes began in October, and gestation appeared in April next year. Spermatophores were discovered for the first time in Sebastes, which assembled in testis, main sperm duct, oviduct and genital tract, as well as ovarian cavity in October and April. These organs may serve either as production or hiding places for spermatophores and spermatozoa which were stored and transported in form of spermatophores. Testicular degeneration started from the distal part of testis in April, with spermatophores assembled in degenerating testis and waiting for transportation. The copulation probably lasted for a long period, during which the spermatozoa were discharged in batches as spermatophores. These spermatophores were coated with sticky materials secreted from the interstitial areas of testis and the main sperm duct, then transported into ovary.展开更多
Aim： To examine the expression and regulation of integral membrane protein 2b （Itm2b） in rat male reproductive tissues during sexual maturation and under different treatments by in situ hybridization. Methods： Tes...Aim： To examine the expression and regulation of integral membrane protein 2b （Itm2b） in rat male reproductive tissues during sexual maturation and under different treatments by in situ hybridization. Methods： Testis, epididymis, and vas deferens were collected on days 1-70 to examine Itm2b expression during sexual maturation. To further examine the regulation of Itm2b, adult rats underwent surgical castration and cryptorchidism. Ethylene dimethane sulfonate and busulfan treatments were carried out to test the regulation of Itm2b after destruction of Leydig cells and germ cells. Results： In testis, Itm2b expression was moderately detected in the adluminal area of seminiferous cords on days 1-10, and detected at a low level in the spermatogonia on days 20 and 30. The Itm2b level was markedly increased in Leydig cells from day 20 to day 70. In epididymis and vas deferens, Itm2b was detected from neonate to adults, and the signal gradually increased in accordance with sexual maturation. Itm2b expression was significantly downregulated in epididymis and vas deferens of castrated rats, and strongly stimulated when castrated rats were treated with testosterone. Cryptorchidism led to a significant decline of Itm2b expression in testis and caput epididymis. Itm2b expression in epididymis and vas deferens was significantly decreased after the Leydig ceils were destroyed by ethylene dimethane sulfonate. Busulfan treatment produced no obvious change in Itm2b expression in epididymis or vas deferens. Conelusion： Our data suggested that Itm2b expression is upregulated by testosterone and might play a role in rat male reproduction.展开更多
[Objective] The aim was to optimized and established the regeneration system of Moringa oleifera and provided foundation for its rapid propagation and further research. [Method] Tissue culture techniques and large sca...[Objective] The aim was to optimized and established the regeneration system of Moringa oleifera and provided foundation for its rapid propagation and further research. [Method] Tissue culture techniques and large scale micropropagation of M. oleifera were studied in this paper. By means of a series of experiments, we used the leaf of aseptic seedling from M. oleifera as explants to optimize and establish the regeneration system cultured in vitro by means of direct organogenesis. [Result] It was observed that using the fresh shelled M. oleifera seeds with 0.1% mercuric chloride for 6 minutes could reach the best disinfection effect. The seed germination rate was 85%. The leaf could produce cluster buds well using the medium with MS＋2.0 mg/L 6-BA＋0.05 mg/L NAA, while the best proliferation condition was under MS＋6-BA 1.0 mg/L＋KT 0.1 rag/L＋2, 4-D 2.0 mg/L＋NAA 0.05 mg/L. The best rooting induction culture medium was MS＋0.5 mg/L IBA, with the rooting rate as 100%. [Cenclusien] This protocol might find use in mass production of true- to-type plants and in production of transgenic plants through Agrobacterium/biolisticmediated transformation.展开更多
Primitive neuroectodermal tumor (PNET) of uterus is the rare of the rarest among the tumors of female genital tract. We herein reported a 32 years old female operated for lower abdominal lump,which was diagnosed,as PN...Primitive neuroectodermal tumor (PNET) of uterus is the rare of the rarest among the tumors of female genital tract. We herein reported a 32 years old female operated for lower abdominal lump,which was diagnosed,as PNET later confirmed by immunohistochemistry (IHC). She was lost to follow-up and presented 10 months later with recurrence. She was treated with cyclical combination chemotherapy followed by definitive surgery and is in complete remission at present.展开更多
The buds of ginger grown in Tongdao Dong Autonomous County were used as explants, the tissue culture and rapid propagation of ginger were studied and the CMV (Cucumber mosaic virus) and TMV (Tobacco mosaic virus) of g...The buds of ginger grown in Tongdao Dong Autonomous County were used as explants, the tissue culture and rapid propagation of ginger were studied and the CMV (Cucumber mosaic virus) and TMV (Tobacco mosaic virus) of ginger tissue culture seedlings were detected. The results showed that MS+6-BA 4.0 mg/L+NAA 0.2 mg/L was the suitable medium for the proliferation induction and differentiation of ginger seedlings. This medium can realize the synchronous culture of ginger seedling proliferation and rooting, and one-step seedling re fining and transplanting, and the proliferation multiple can reach 3.00. The suitable rooting medium was 1/2MS+0.4 mg/L NAA. The survival rate of coir and vermiculite was the highest among the 4 culture mediums, reaching 100%. The survival rate of peat and cottonseed shell was very low, among which the ginger seedling transplanted with vermiculite grew more robustly than that transplanted with coir, with developed root system, more adventitious roots and less yellowing of leaves. No CMV and TMV were found in the tissue culture seedlings of ginger. The tissue culture seedlings can be used for the production of non-toxic ginger seedlings.展开更多
The behavior of industrial cluster is similar to the behavior of biological population, that is, the industrial cluster is seemed to be an ecological symbiotic system. The relationship between economic organizations i...The behavior of industrial cluster is similar to the behavior of biological population, that is, the industrial cluster is seemed to be an ecological symbiotic system. The relationship between economic organizations in the industrial cluster is similar to the relationship of species in ecological system, and symbiotic reciprocity, competition and cooperation are the basic. The ecological development is mainstream and direction of industrial cluster. In this paper, firstly, we studied the ecological symbiosis growth model of industry cluster, and tbund it being according to logistic growth model; secondly, we studied the growth and reproduction of economic organizations in industrial cluster in three different cases, which with only competition relations, only beneficial relations, and both competition and beneficial cooperation relationship between economic organizations, and finally, we determined the number of economical organizations when the industrial cluster attained ecological equilibrium.展开更多
The presence of sperm was observed in different organs of the reproductive tract of M. schmitti. These were the club, baffle and terminal zone of the oviducal gland, posterior portion of the uterus and in the cervix. ...The presence of sperm was observed in different organs of the reproductive tract of M. schmitti. These were the club, baffle and terminal zone of the oviducal gland, posterior portion of the uterus and in the cervix. This fact allowed for consideration not only its storage but its interaction with the female reproductive tract epithelia as well. SEM （microscopic observation studies） detected loose and sole spermatozoa in the club and baffle zones in a pregnant female. On the other hand, histochemical techniques showed sperm storage tubules of the terminal zone, which contain bundles of sperm, do not stain AB （alcian blue） or PAS （periodic acid schiff）. Disintegration of some of the male gametes was observed in the posterior part of the uterus with TEM （transmission electron microscopy） studies which would indicate a first uterine sperm selection. The uterine epithelial cells involved in this process showed an important level of secretion vacuoles suggesting an interaction with sperm cells. Sperm in the cervix was found without the matrix that constitutes the spermatozeugmata formed in the male tract. Some heads of the sperm were uncurled while they were interacting with the cervical cells.展开更多
基金Supported by Beijing Natural Science Foundation (6072005)Funding Project for Academic Human Resources Development in Institutions of Higher Learning Under the Jurisdiction of Beijing Municipality(PXM2009-014207-076874)~~
文摘[Objective]The aim was to commercialize micropropagation of pistachio and provide good foundations for varieties improvement. [Method]The seeds and stem-segments were used as explants in the tissue culture of Pistacia vera ＇Kerman＇,tissue culture condition and medium and transplant matrix were researched for micropropagation of pistachio,[Result]Mediums of 1/2DKW and 1/2DKW＋3.00 mg/L of 6-BA ＋0.05 mg/L of NAA were suitable medium for seed germination and axillary bud induction of pistachio; Proliferation coefficient was 3.6 on the medium of 1/2DKW ＋4.00 mg/L of 6-BA ＋ 0.05 mg/L of IBA; Rooting rate was up to 75% on the medium of 1/2DKW ＋ 5.00 mg/L of IBA ＋ 1.00 mg/L of NAA; The suitable transplantation matrix was 3/4 sand ＋ 1/4 vermiculite,and survival rate of plant with root was above 79%. [Conclusion]The pistachio （Kerman） tissue culture technology system was established.
基金Supported by Students’S&T Innovation Project of Zhejiang Colleges and Universities(2015R463001)~~
文摘The research explored rapid multiplication on Bletilla striata and the re- suits showed that the medium of MS＋2.5 mg/L6-BA＋0.2 mg/L NAA performed the best in induction, with induction rate at 98%; the medium with MS＋IAA2.5mg/L＋ 2.5 mg/L6-BA＋2.0 mg/L NAA was the best in terms of induction and multiplication; the medium with 1/2MS＋0.01 mg/L NAA 1＋0.01 mg/L IAA promoted rooting.The harden- ing temperature was the optimal at 23℃-28 ℃ and relative humidity of 80%-90% the best.
基金Supported by Taizhou College Students’Innovative Team Funding Project(classⅠ)~~
文摘[Objective] This study was conducted to acquire a large quantity of wide raspberry plantlets and provide a basis for large-scale production. [Method] Re- search was performed on series tissue culture propagation of wide raspberry. [Re- sult] Adventitious shoots were induced with stem segments, and the best induction medium was MS ＋1.5 mg/L BA ＋0.3 mg/L NAA with an induction rate of 96%; and the best differentiation medium was MS ＋2.0 mg/L KT ＋0.5 mg/L NAA, which ex- hibited high root number and quality, with an average root number of 4.5 per plant. The substratum for training plantlets was leaf mould 60%＋perlite 30%＋vermiculite 10%; and the temperature of 23-27℃ and relative air humidity of 80%-90% were beneficial to survival of trained plantlets.[Conclusion] This study laid a foundation for further large-area cultivation of Rubus chingii Hu.
基金supported financially by Grand Innovating Program of Agriculture Applying Technique in Shandong Province (No. 2008-109)Modern Agricultural Industry Technology System in Shandong Province and the Science and Technology Development Program of Yantai (No. 2013 ZH088)
文摘Black rockfish(Sebastes schlegeli) is an important species for culture; however, its reproductive characteristics have not been fully documented. In this study, we investigated the morphology and developmental process of germ cells in this ovoviviparous rockfish in reproductive season(October 2011–November 2012) with histological methods. We found that the gonad of mature fish showed notable seasonal changes in developmental characteristics and morphological structure. The sperm cells matured during a period lasting from October to December, significantly earlier than the oocytes did. A large number of spermatozoa and other cells occurred in testis at different developmental stages. Vitellogenesis in oocytes began in October, and gestation appeared in April next year. Spermatophores were discovered for the first time in Sebastes, which assembled in testis, main sperm duct, oviduct and genital tract, as well as ovarian cavity in October and April. These organs may serve either as production or hiding places for spermatophores and spermatozoa which were stored and transported in form of spermatophores. Testicular degeneration started from the distal part of testis in April, with spermatophores assembled in degenerating testis and waiting for transportation. The copulation probably lasted for a long period, during which the spermatozoa were discharged in batches as spermatophores. These spermatophores were coated with sticky materials secreted from the interstitial areas of testis and the main sperm duct, then transported into ovary.
基金Acknowledgment This work was supported by grants from National Basic Research Program of China （No. 2006CB504005 and No. 2006CB944009） and National Natural Science Foundation of China （No. 30330060 and No. 30570198）.
文摘Aim： To examine the expression and regulation of integral membrane protein 2b （Itm2b） in rat male reproductive tissues during sexual maturation and under different treatments by in situ hybridization. Methods： Testis, epididymis, and vas deferens were collected on days 1-70 to examine Itm2b expression during sexual maturation. To further examine the regulation of Itm2b, adult rats underwent surgical castration and cryptorchidism. Ethylene dimethane sulfonate and busulfan treatments were carried out to test the regulation of Itm2b after destruction of Leydig cells and germ cells. Results： In testis, Itm2b expression was moderately detected in the adluminal area of seminiferous cords on days 1-10, and detected at a low level in the spermatogonia on days 20 and 30. The Itm2b level was markedly increased in Leydig cells from day 20 to day 70. In epididymis and vas deferens, Itm2b was detected from neonate to adults, and the signal gradually increased in accordance with sexual maturation. Itm2b expression was significantly downregulated in epididymis and vas deferens of castrated rats, and strongly stimulated when castrated rats were treated with testosterone. Cryptorchidism led to a significant decline of Itm2b expression in testis and caput epididymis. Itm2b expression in epididymis and vas deferens was significantly decreased after the Leydig ceils were destroyed by ethylene dimethane sulfonate. Busulfan treatment produced no obvious change in Itm2b expression in epididymis or vas deferens. Conelusion： Our data suggested that Itm2b expression is upregulated by testosterone and might play a role in rat male reproduction.
基金National Natural Science Foundation of China(21165008)~~
文摘[Objective] The aim was to optimized and established the regeneration system of Moringa oleifera and provided foundation for its rapid propagation and further research. [Method] Tissue culture techniques and large scale micropropagation of M. oleifera were studied in this paper. By means of a series of experiments, we used the leaf of aseptic seedling from M. oleifera as explants to optimize and establish the regeneration system cultured in vitro by means of direct organogenesis. [Result] It was observed that using the fresh shelled M. oleifera seeds with 0.1% mercuric chloride for 6 minutes could reach the best disinfection effect. The seed germination rate was 85%. The leaf could produce cluster buds well using the medium with MS＋2.0 mg/L 6-BA＋0.05 mg/L NAA, while the best proliferation condition was under MS＋6-BA 1.0 mg/L＋KT 0.1 rag/L＋2, 4-D 2.0 mg/L＋NAA 0.05 mg/L. The best rooting induction culture medium was MS＋0.5 mg/L IBA, with the rooting rate as 100%. [Cenclusien] This protocol might find use in mass production of true- to-type plants and in production of transgenic plants through Agrobacterium/biolisticmediated transformation.
文摘Primitive neuroectodermal tumor (PNET) of uterus is the rare of the rarest among the tumors of female genital tract. We herein reported a 32 years old female operated for lower abdominal lump,which was diagnosed,as PNET later confirmed by immunohistochemistry (IHC). She was lost to follow-up and presented 10 months later with recurrence. She was treated with cyclical combination chemotherapy followed by definitive surgery and is in complete remission at present.
文摘The buds of ginger grown in Tongdao Dong Autonomous County were used as explants, the tissue culture and rapid propagation of ginger were studied and the CMV (Cucumber mosaic virus) and TMV (Tobacco mosaic virus) of ginger tissue culture seedlings were detected. The results showed that MS+6-BA 4.0 mg/L+NAA 0.2 mg/L was the suitable medium for the proliferation induction and differentiation of ginger seedlings. This medium can realize the synchronous culture of ginger seedling proliferation and rooting, and one-step seedling re fining and transplanting, and the proliferation multiple can reach 3.00. The suitable rooting medium was 1/2MS+0.4 mg/L NAA. The survival rate of coir and vermiculite was the highest among the 4 culture mediums, reaching 100%. The survival rate of peat and cottonseed shell was very low, among which the ginger seedling transplanted with vermiculite grew more robustly than that transplanted with coir, with developed root system, more adventitious roots and less yellowing of leaves. No CMV and TMV were found in the tissue culture seedlings of ginger. The tissue culture seedlings can be used for the production of non-toxic ginger seedlings.
文摘The behavior of industrial cluster is similar to the behavior of biological population, that is, the industrial cluster is seemed to be an ecological symbiotic system. The relationship between economic organizations in the industrial cluster is similar to the relationship of species in ecological system, and symbiotic reciprocity, competition and cooperation are the basic. The ecological development is mainstream and direction of industrial cluster. In this paper, firstly, we studied the ecological symbiosis growth model of industry cluster, and tbund it being according to logistic growth model; secondly, we studied the growth and reproduction of economic organizations in industrial cluster in three different cases, which with only competition relations, only beneficial relations, and both competition and beneficial cooperation relationship between economic organizations, and finally, we determined the number of economical organizations when the industrial cluster attained ecological equilibrium.
文摘The presence of sperm was observed in different organs of the reproductive tract of M. schmitti. These were the club, baffle and terminal zone of the oviducal gland, posterior portion of the uterus and in the cervix. This fact allowed for consideration not only its storage but its interaction with the female reproductive tract epithelia as well. SEM （microscopic observation studies） detected loose and sole spermatozoa in the club and baffle zones in a pregnant female. On the other hand, histochemical techniques showed sperm storage tubules of the terminal zone, which contain bundles of sperm, do not stain AB （alcian blue） or PAS （periodic acid schiff）. Disintegration of some of the male gametes was observed in the posterior part of the uterus with TEM （transmission electron microscopy） studies which would indicate a first uterine sperm selection. The uterine epithelial cells involved in this process showed an important level of secretion vacuoles suggesting an interaction with sperm cells. Sperm in the cervix was found without the matrix that constitutes the spermatozeugmata formed in the male tract. Some heads of the sperm were uncurled while they were interacting with the cervical cells.
