从科学视角审视健身气功与武术的协同作用

该文从科学视角探讨健身气功与武术的协同作用。在起源上,武术、健身气功融合了技击与养生。明清时,武术为民间养生技术,武术门派兴以气功为基。在日常生活中,健身气功对唱歌、爬山等有益,而武术多以艺术的形式展现,借助新媒体拓宽学习渠道,便于人们参与,有助于提升人们的身心健康水平。健身气功与武术二者在提高人体机能方面相似。在与养生的关系方面,健身气功调身、调心、调息,传统武术可提高内功修炼和身体素质,注重内外修养,并结合阴阳五行学说进行创新。健身气功与武术二者协同为传承文化和提升健康水平开辟了新的路径。

浅谈高考语文对教学“生活化”导向作用的落实

高考语文命题取材贴近生活,极富有时代气息,对高中语文教学具有“生活化”的导向作用。语文教学要落实这个作用,一是课堂教学要有大语文观,将时代活水引入课堂,落实“生活化”导向作用,二是要把课堂扩大到图书馆、社会,落实“生活化”导向作用,三是教学设计教学评价要有弹性,落实“生活化”导向作用。

分析健康教育在冠心病心绞痛患者护理中的应用及对改善不良生活方式的作用

探究将健康教育实施到冠心病心绞痛患者的护理中的应用价值以及对患者不良生活方式的改善作用。方法:取78例冠心病心绞痛患者,截取的时间区间为:2020年3月至2021年4月范围,经由双盲法原则完成组间划分,即对照组(纳入样本39例,所行为常规护理)、观察组(纳入样本39例,所行为健康教育护理),就其不良生活方式改变情况、健康知识掌握度评分以及生活质量展开调查。结果:观察组的不良生活方式发生率调查数据结果相较于对照组更低(P<0.05),健康知识掌握度得分与生活质量评分均同比于对照组更高(P<0.05)。结论:将健康教育护理理念应用于冠心病心绞痛患者的临床护理中可以有效提高患者对疾病的认知,提高其自身对疾病的预防心理,改善其不良生活方式,缓解病情,提高其生活质量,具有较高的临床参考价值。

绿色节能建筑施工对环境污染的改善作用探讨

在中国经济社会发展的主导产业中,建筑业一直扮演着举足轻重的角色,但是以往的建筑总是忽略了环境污染、土地资源耗费等客观因素,而谋求了更大的经济效益。随着社会消费观念的演变和人们对节能环保的日益重视,建筑行业若要实现可持续的稳定发展,就必须进行绿色、节能的创新和改革,因此提出了绿色节能建筑设计的理念。在建筑工程设计中应用绿色理念不仅能有效提升建筑施工,还可以为人们提供更为舒适便捷的居住环境,促进人与自然和谐共处。利用巧妙的施工布局,达到施工资源与结构的充分融合,进而提升资源效率,降低施工过程中对周边环境产生的环境污染。在建筑中加入了绿色概念,并引入更加先进的施工技术设备,以达到低能耗的目标,这也和中国对现代化与可持续建设的要求不谋而合。

难治性癫痫药物治疗患者干预作用效果观察

目的:观察心理干预对卡马西平治疗癫痫患者的临床增效作用。方法:将72例癫痫患者按就诊顺序分为两组,各36例,观察组采用心理干预合并卡马西平治疗,对照组单纯采用卡马西平治疗,6个月后对临床疗效及生活质量进行评定。结果:采用SPSS13.0进行统计分析。结果观察组控制5例、显效9例、有效16例、无效3例、加重1例,有效率88.24%,对照组分别为2例、6例、15例、8例、2例,有效率69.70%,两组比较差异有显著性意义(P<0.05)。两组的生活质量评分与治疗前比较差异均有显著性意义(P<0.01),组间比较差异有显著性意义(P<0.01)。结论:心理干预对卡马西平治疗癫痫患者具有临床增效及提高生活质量的作用。

邹韬奋《生活日报》的办报经历

邹韬奋一生盼望着能够办一份适合于大众需要的日报,但是他的日报理想仅仅通过《生活日报》在香港生存了55天,而这也是邹韬奋一生中办的唯一一份日报。是什么原因让邹韬奋远走香港实现日报理想?又是什么原因让他的理想仅仅存活了55天?这短暂的出版活动又呈现出了何种样态?本文试从当时的社会历史图景中,挖掘出作用于邹韬奋出版活动的因素,并梳理它们之间的相互关系,以此呈现邹韬奋日报理想与客观现实的相互作用。

依达拉奉联合苦碟子对急性脑梗死神经功能缺损症状的改善作用

【目的】观察依达拉奉联合苦碟子治疗急性脑梗死对神经功能缺损症状的改善作用。【方法】将92例急性脑梗死患者随机分为治疗组和对照组各46例。2组均给予常规治疗(包括肠溶阿司匹林和神经营养剂的使用,大面积脑梗死时降颅压及对症处理)和合并症处理,治疗组加用依达拉奉注射液联合苦碟子注射液静脉滴注,对照组仅予苦碟子注射液静脉滴注。2组均以14 d为1个疗程,共治疗2个疗程。于治疗前和治疗第1、第2疗程后分别进行神经功能缺损评定(ESS)和日常生活能力评分(ADL),并随时记录不良反应。【结果】第1疗程结束后,2组的ESS、ADL积分值均有所改善(与治疗前比较,P<0.05或P<0.01),但2组的ESS、ADL积分值和增分率比较,差异均无显著性意义(P>0.05)。第2疗程结束后,2组的ESS、ADL积分值均有明显改善(与治疗前比较,P<0.01),且2组的ESS、ADL积分值和增分率比较,差异均有显著性意义(P<0.05)。【结论】依达拉奉联合苦碟子治疗急性脑梗死在神经功能改善方面优于单用苦碟子。

论共产党人的五种作风建设——学习十五届六中全会《决定》的体会

党的十五届六中全会通过的《关于加强和改进党的作风建设的决定》明确提出:“当前和今后一个时期,要抓住重点,集中解决党的思想作风、学风、工作作风、领导作风和干部生活作风方面的突出问题。”重点抓这“五种作风”建设,抓住了新形势下推进党的建设新的伟大工程的一个十分重要的环节,抓住了提高党的领导水平和执政水平。

Expression and Identification of Mycoplasma penetrans P35 Lipoprotein

Objective: To study the biological activity of Myco-plasma penetrans 35kDa lipoprotein(P35) in vitro, prokaryotic expression vector pQE31//p35 was constructed and recombinant fusion protein P35 (rP35) was expressed in E.coli. Methods: The p35 gene was amplified by polymerase chain reaction(PCR), cloned to pQE31, and a positive clone was screened. PCR-mediated mutagenesis was used to change the two "TGA" triplets to "TGG" triplets within the p35 gene. Production of the recombinant protein was induced by the addition of IPTG to the E.coli culture. rP35 was purified with a Ni-NTA Spin Kit and rP35 purification was analyzed by Western blot. Results: About 1Kb PCR amplification was cloned into pQE31. The two "TGA" triplets within the p35 gene were successfully changed to "TGG" triplets. The pQE31/p35 vector expressed a protein with a calculated molecular mass of 37.4kDa in E.coli. Western blot indicated the 37.4kDa protein was rP35 . Conclusion: PQE31/p35, a prokaryotic expression vector containing p35 gene, was successfully constructed and expressed in E.coli.

新教育理念下的高职德育新方法

高职德育是高职教育阶段推行的德育活动，旨在培养高技能应用型人才所具备的优秀道德品质和良好的职业心理素质的教育活动。当前，高职的德育工作存在着一些问题，德育时效性较差，德育工作陷入困境。而如何将道德教育深入高职学生的心灵并转化为学生的道德自觉是值得德育工作者深思的重要问题。本文提出必须认真学习贯彻科学发展观。以人为本，与时俱进地创新高职德育工作。

Partial Nitrification from Domestic Wastewater by Aeration Control at Ambient Temperature

The objective of this paper was to examine the feasibility of partial nitrification from raw domestic wastewater at ambient temperature by aeration control only. Airflow rate was selected as the sole operational parameter. A 14L sequencing batch reactor was operated at 23℃ for 8 months, with an input of domestic wastewater. There was a prolgrammed decrease of the airflow rate to 28L·h^-1, the corresponding average dissolved oxygen （DO） was 0.32mg·h^-1, and the average nitrite accumulation rate increased to 92.4% in 3 weeks. Subsequently, further increase in the airflow rate to 48L·h^-1 did not destroy the partial nitrification to nitrite, with average DO of 0.60mg·h^-1 and nitrite accumulating rate of 95.6%. The results showed that limited airflow rate to cause oxygen deficiency in the reactor would eventually induce only nitrification to nitrite and not further to nitrate and that this system showed relatively stability at higher airflow rate independent of pH and temperature. About 50% of influent total nitrogen was eliminated coupling with partial nitrification, taking the advantage of low DO during the reaction.

Effect of Pre-oxidation on the Properties of Crushed Bituminous Coal and Activated Carbon Prepared Therefrom

The influence of a pre-oxidation process on the chemical properties of crushed bituminous coal and on adsorption properties of the subsequently formed char and activated carbon is discussed in this paper. Datong bituminous coal samples sized 6 mm were oxidized at different temperatures and for different times and then carbonized and activated by steam to obtain activated carbons. A Uniform Design method was used to arrange the experiments,IR and adsorption experiments were used to characterize these oxidized coals,chars and activated carbon samples. The results show that the carboxyl group disappeared and α-CH2 groups joined to alkenes decreased dramatically but the carbonyl group clearly increased in the coal sample oxidized at 543 K; The chemical composition of coal samples oxidized at lower temperature is different from that of coal oxidized at 543 K. Oxidizing coal samples at higher temperatures for a short time or at lower temperatures for a longer time resulted in activated carbon samples that tended toward the same adsorption properties: Iodine number 1100 mg/g and Methylene blue value 252 mg/g. The yield of activated carbon obtained from the pre-oxidized coal is 10% higher than the yield from parent coal but the activated carbons have the same adsorption properties.

Effects of rice straw on the cell viability, photosynthesis, and growth of Microcystis aeruginosa

Rice straw is supposed to be an environment-friendly biomaterial for inhibiting the growth of harmful blooms of the cyanobacterium Microcystis aeruginosa. However, its potential mechanism is not well known. To explore this mechanism, the growth, cell viability(esterase activity, membrane potential, and membrane integrity), photosynthesis, and cell size of M. aeruginosa were determined using fl ow cytometry and Phyto-PAM after exposure to rice straw extracts(RSE). The results show that doses from 2.0 to 10.0 g/L of RSE effi ciently inhibited the alga for 15 days, while the physiologic and morphologic responses of the cyanobacteria were time-dependent. RSE interfered with the cell membrane potential, cell size, and in vivo chlorophyll- a fl uorescence on the fi rst day. After 7 days of exposure, RSE was transported into the cytosol, which disrupted enzyme activity and photosynthesis. The cyanobacteria then started to repair its physiology(enzyme activity, photosynthesis) and remained viable, suggesting that rice straw act as an algistatic agent.

Regulation of gene expression, growth, and cell survival by IL-4: Contribution of multiple signaling pathways

Interleukin-4 is a cytokine produced by activated T cells, mast cells, and basophils that elicits many important biological responses[1] (see Tab 1). These responses range from the regulation of helper T cell differentiation[2] and the production of IgE[3] to the regulation of the adhesive properties of endothelial cells via VCAM-1[4]. In keeping with these diverse biological effects, high-affinity binding sites for IL-4 (Kd 20 to 300 pM) have been detected on many hematopoietic and non-hematopoietic cell types at levels ranging from 50 to 5000 sites per cell[5],This review will focus on the discrete signal transduction pathways activated by the IL-4 receptor and the coordination of these individual pathways in the regulation of a final biological outcome.

The clinical effects of DC-CIK cells combined with chemotherapy in the treatment of advanced NSCLC

Objective: The aim of the study was to evaluate the safety and therapeutic effects of autologous dendritic cells co-cultured with cytokine-induced killer cells (DC-CIK) combined with chemotherapy in advanced non-small cell lung cancer (NSCLC) patients. Methods: Fifty patients with advanced NSCLC (stages III to IV), who had received therapies in our Center (Department of Biotherapy, Affiliated to Cancer Hospital of Shanxi Medical University, Taiyuan, China) from August 2008 to January 2010, were treated by DC-CIK + chemotherapy as the combined treatment group; fifty advanced NSCLC patients treated with chemotherapy at the same time served as controls. The immunologic function, short-term therapeutic effects, the 1-year survival rate, the life quality, the chemotherapy side effects were compared between the two groups, the safety and therapeutic effects of DC-CIK cells therapy were observed too. Results: There was no obvious change of subsets of T cells in peripheral blood before and after therapy in DC-CIK + chemotherapy group, and IFN-γ was improved after therapy in this group (P < 0.05); in chemotherapy alone group, the ratios of CD3+CD4+, CD3+CD8+, CD3-CD56+ cells and the secretion of IL-2, TNF-α decreased significantly after therapy (P < 0.05); the ratios of CD3+CD8+, CD3+CD56+ were improved after cell culture (P < 0.05). The disease control rate (DCR) of DC-CIK + chemotherapy group was higher than that in the chemotherapy alone group (78.0% vs 56.0%, P < 0.05); the 1-year survival rates of DC-CIK + chemotherapy group and chemotherapy alone group were 50% and 44% respectively, had no significant difference. Compared with chemotherapy alone group, the occurrence of chemotherapy side effects (including bone marrow suppression, nausea and vomiting, peripheral nerve toxicity) was less in the DC-CIK + chemotherapy group (P < 0.05). The physical and appetite were better in DC-CIK + chemotherapy group after therapy. Conclusion: To compare with simple chemotherapy, DC-CIK + chemotherapy for advanced NSCLC is safe and effective, and it can improve patients' life quality and remission rate, and prolong their survival time.

Biological activities of a neutral water-soluble agar polysaccharide prepared by agarase degradation

Depolymerization of agar was performed using agarase, which was extracted from the cell-free medium of a culture of marine bacterial Alterornonas sp. nov. SY 37-12. After ethanol fractionation and lyophilization, the water-soluble agar polysaccharide （WSAP3） was collected. The anti-tumor activity of the product was determined by using Sarcoma 180 tumor in mouse. The tumor inhibition rate of WSAP3 the product was determined by using Sarcoma 180 tumor in mouse. The tumor inhibition rate of WSAP3 reached 48.7% at a dose of 64mg kg^-1 after 15 days treatment. WSAP3 enhanced the aetivities of superoxide dismutase and catalase, which suggests that WSAP3 was effective in promoting the antioxidation ability and eliminating danger from free radicals. The result of flow cytometry showed that the WSAP3 had no activities of cell cycle inhibition or apoptosis-inducing activities. The anti-oxidation of WSAP3 was further confirmed by test in vitro, which might play an important role in anti-tumor activity. The immunological regulation of WSAP3, especially its effect on the phagocytosis ratio and phagocytosis index of rophage was also assayed in test in vivo.

EFFECT OF PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR ACTIVATORS ON TUMOR NECROSIS FACTOR-αEXPRESSION IN NEONATAL RAT CARDIAC MYOCYTES

Objective To investigate the effect of peroxisome proliferator-activated receptor-α(PPARα) and PPARγactivators on tumor necrosis factor-α(TNFα) expression in neonatal rat cardiac myocytes. Methods Primary cultures of cardiac myocytes from 1- to 3-day-old Wistar rats were prepared, and myocytes were ex-posed to lipopolysaccharide (LPS) and varying concentrations of PPARαor PPARγactivator (fenofibrate or pioglitazone).RT-PCR and ELISA were used to measure TNFα, PPARα, and PPARγexpression in cultured cardiac myocytes. Transient tr-ansfection of TNFαpromoter with or without nuclear factor-kappaB (NF-κB) binding site to cardiac myocytes was performed. Results Pretreatment of cardiac myocytes with fenofibrate or pioglitazone inhibited LPS-induced TNFαmRNA and protein expression in a dose-dependent manner. However, no significant changes were observed on PPARαor PPARγmRNA expression when cardiac myocytes were pretreated with fenofibrate or pioglitazone. Proportional suppression of TNFαpromoter activity was observed when myocytes was transiently transfected with whole length of TNFαpromoter (-721/+17) after being stimulated with LPS and fenofibrate or pioglitazone, whereas no change of promoter activity was observed with transfection of TNFαreporter construct in deletion of NF-κB binding site (-182/+17). Conclusions PPARαand PPARγactivators may inhibit cardiac TNFαexpression but not accompanied by change of PPARαor PPARγmRNA expression. Therefore PPARαand PPARγactivators appear to play a role in anti-inflammation. The mechanism may partly be involved in suppression of the NF-κB pathway.

Combination of telomerase antisense oligonucleotides simultaneously targeting hTR and hTERT produces synergism of inhibition of telomerase activity and growth in human colon cancer cell line

AIM: To investigate synergism of inhibition of telomerase activity and proliferation of human colon cancer cells by combination of telomerase antisense oligonucleotides (ASODNs) simultaneously targeting human telomerase RNA (hTR) and human telomerase reverse transcriptase (hTERT) in vitro. METHODS: ASODN of hTR and ASODN of hTERT were transfected into human colon cancer SW480 cells by liposomal transfection reagents. Telomerase activity of SW480 cells was examined using telomeric repeat amplification protocol (TRAP)-enzyme-linked immunosorbent assay (PCR-ELISA). Proliferation activity of SW480 cells was tested by methyl thiazolyl tetrazolium assay. Apoptosis and cell cycle were analyzed by flow cytometry. RESULTS: The telomerase activity and cell survival rate in SW480 cells transfected with 0.2 μmol/L of ASODN of hTR or ASODN of hTERT for 24-72 h were significantly decreased in a time-dependent manner compared with those after treatment with sense oligonucleotides and untreated (telomerase activity: 24 h, 73%, 74% vs99%, 98%; 48 h, 61%, 55% vs98%, 99%; 72 h, 41%, 37% vs 99%, 97%; P<0.01; cell survival rate: 24 h, 88%, 86% vs594%, 98%; 48 h, 49%, 47% vs94%, 97%; 72 h, 44%, 42% vs92%, 96%; P<0.01). Moreover, the telomerase activity and the cell survival rate in SW480 cells treated by the combination of telomerase anti-hTR and anti-hTERT were more significantly suppressed than single anti-hTR or anti-hTERT (telomerase activity: 24 h, 59% vs 73%, 74%; 48 h, 43% vs61%, 55%; 72 h, 18% vs41%, 37%; P<0.01; cell survival rate: 24 h, 64% vs88%, 86%; 48 h, 37% vs49%, 47%; 72 h, 25% vs44%, 42%; P<0.01). Meanwhile, the apoptosis rates in the combination group were markedly increased compared with those in the single group (24 h, 18.0% vs7.2%, 7.4%; 48 h, 23.0% vs13.0%, 14.0%; 72 h, 28.6% vs 13.2%, 13.75; P<0.01). Cells in combination group were arrested at G0/G1 phase. CONCLUSION: Telomerase anti-hRT and anti-hTERT suppress telomerase activity, and inhibit growth of human colon cancer cells probably via induction of apoptosis and retardation of cell cycle. Additionally, combined use of telomerase ASODNs targeting both hTR and hTERT yields synergistic action selective for human colon cancer.

Tetrandrine inhibits activation of rat hepatic stellate cells in vitro via transforming growth factor-β signaling

AIM: To investigate the effect of various concentrations of tetrandrine on activation of quiescent rat hepatic stellate cells (HSCs) and transforming growth factor-β (TGF-β) signaling in vitro.METHODS: HSCs were isolated from rats by in situperfusion of liver and 18% Nycodenz gradient centrifugation, and primarily cultured on uncoated plastic plates for 24 hwith DMEM containing 20% fetal bovine serum (FBS/DMEM) before the culture medium was substituted with 2% FBS/DMEM for another 24 h. Then, the HSCs were cultured in 2% FBS/DMEM with tetrandrine (0.25, 0.5, 1,2 mg/L, respectively). Cell morphological features were observed under an inverted microscope, smooth muscleα-actin (α-SMA) was detected by immunocytochemistry and image analysis system, laminin (LN) and type Ⅲprocollagen (PCⅢ) in supernatants were determined byradioimmunoassay. TGF-β1 mRNA, Smad 7 mRNA and Smad 7 protein were analyzed with RT-PCR and Western blotting, respectively.RESULTS: Tetrandrine at the concentrations of 0.25-2 mg/L prevented morphological transformation of HSC from the quiescent state to the activated one, while α-SMA, LN and PCⅢ expressions were inhibited. As estimated by gray values, the expression of α-SMA in tetrandrine groups (0.25, 0.5, 1, 2 mg/L) was reduced from 21.3% to 42.2%(control: 0.67, tetrandrine groups: 0.82, 0.85, 0.96, or 0.96, respectively, which were statistically different from the control, P＜0.01), and the difference was more significant in tetrandrine at 1 and 2 mg/L. The content of LN in supernatants was significantly decreased in tetrandrine groups to 58.5%, 69.1%, 65.8% or 60.0% that of the control respectively, and that of PCⅢ to 84.6%, 81.5%,75.7% or 80.7% respectively (P＜0.05 vs control), with no significant difference among tetrandrine groups. RTPCR showed that TGF-β1 mRNA expression was reduced by tetrandrine treatments from 56.56% to 87.90% in comparison with the control, while Smad 7 mRNA was increased 1.4-4.8 times. The TGF-β1 mRNA and Smad 7 mRNA expression was in a significant negative correlation (r= -0.755, P＜0.01), and both were significantly correlated with α-SMA protein expression (r = -0.938, P＜0.01;r = 0.938, P＜0.01, respectively). The up-regulation of Smad 7 protein by tetrandrine (1 mg/L)was confirmed by Western blotting as well.CONCLUSION: Tetrandrine has a direct inhibiting effect on the activation of rat HSCs in culture. It up-regulates the expression of Smad 7 which in turn blocks TGF-β1 expression and signaling.

Bioactive compounds of sea cucumbers and their therapeutic effects

Abstract Sea cucumbers belong to the Class Holothuroidea of marine invertebrates. They are commercially valuable and prized as a food and folk medicine in Asia. Nutritionally, sea cucumbers have an impressive profile of valuable nutrients such as vitamins, minerals and amino acids. A number of unique biological and pharmacological activities/properties, including anticancer, anticoagulant/antithrombotic, antimicrobial, antioxidant, antihyperlipidemic, antihyperglycemic, anti-inflammatory, antihypertension and radioprotective, have been ascribed to various compounds isolated from sea cucumbers. The therapeutic properties and medicinal benefits of sea cucumbers can be linked to the presence of a wide array ofbioactives, especially triterpene glycosides, acid mucopolysaccharide, sphingoid bases, glycolipids, fucosylated chondroitin sulfate, polysaccharides, phospholipids, cerebrosides, phosphatidylcholines, and other extracts and hydrolysates. This review highlights the valuable bioactive components as well as the multiple therapeutic properties of sea cucumbers with a view to exploring their potential uses as functional foods and a natural source of new multifunctional drugs.