This paper develops a high time-resolution optimal power generation mix model in its time resolution of 10 minutes on 365 days by linear programming technique. The model allows us to analyse the massive deployment of ...This paper develops a high time-resolution optimal power generation mix model in its time resolution of 10 minutes on 365 days by linear programming technique. The model allows us to analyse the massive deployment of photovoltaic system and wind power generation in power system explicitly considering those short-term output variation. PV （photovoltaic） and wind output are estimated, employing meteorological database. Simulation results reveal that variable fluctuation derived from a high penetration level of those renewables is controlled by quick load following operation of natural gas combined cycle power plant, pumped-storage hydro power, stationary NAS （sodium and sulfur） battery and the output suppression control of PV and wind. It additionally turns out that the operational configuration of those technologies for the renewable variability differs significantly depending on those renewable output variations in each season and solving the seasonal electricity imbalance as well as the daily imbalance is important if variable renewables are massively deployed.展开更多
Objective To construct the recombinant adenovirus vector carrying human growth hormone secretagogue receptor type 1a （GHS-R1a） ,for genetic transfection.Methods The full-length human GHS-R1a gene was obtained by PCR...Objective To construct the recombinant adenovirus vector carrying human growth hormone secretagogue receptor type 1a （GHS-R1a） ,for genetic transfection.Methods The full-length human GHS-R1a gene was obtained by PCR amplification and then cloned into the shuttle plasmid pAdTrack-CMV.The linearized plasmid pAdTrack-CMV-GHS-R1a was co-transformed into Escherichia coli （E.coli） BJ5183 cells along with an adenoviral backbone plasmid pAdEasy1.The HEK293 cells were then infected with adenoviruses.The expression of GHS-R1a was indicated by green fluorescent protein （GFP） ,and confirmed by Reverse Transcription Polymerase Chain Reaction （RT-PCR） and Western blot.Results Enzymatic digestion of pAdGHS-R1a yielded a large fragment （approximately 30 kb） and a small fragment （4.5 kb） ,indicating the success-ful construction of recombinant adenovirus expression vector.Expression of GFP was observed by confocal laser scanning microscopy at 24 h after infection.RT-PCR and Western blot further confirmed that GHS-R1a was efficiently expressed in 293 cells.Conclusion Recombinant adenovirus （AdGHS-R1a） is successfully constructed,and the target gene can be expressed efficiently in 293 cells,which provide a valuable tool for further studying the function of GHS-R1a.展开更多
Water is a crucial factor influencing eco-environment conservation in the Loess Area in China. Soilmoisture is also an indispensable factor to plant growth because of limited water supply. In this paper,previous studi...Water is a crucial factor influencing eco-environment conservation in the Loess Area in China. Soilmoisture is also an indispensable factor to plant growth because of limited water supply. In this paper,previous studies of soil moisture are summarized from methods and contents. Meanwhile, some problems inthe relevant researches are pointed out and discussed. Some solutions are brought forward.展开更多
Aquatic and semi-aquatic mammals, while resting at the water surface or ashore, breathe with a low frequency （f） by comparison to terrestrial mammals of the same body size, the difference increasing the larger the s...Aquatic and semi-aquatic mammals, while resting at the water surface or ashore, breathe with a low frequency （f） by comparison to terrestrial mammals of the same body size, the difference increasing the larger the species. Among various interpretations, it was suggested that the low-f breathing is a consequence of the end-inspiratory breath-holding pattern adopted by aquatic mammals to favour buoyancy at the water surface, and evolved to be part of the genetic makeup. If this interpretation was correct it could be expected that, differently from f, the heart rate （HR, beats/min） of aquatic and semi-aquatic mammals at rest would not need to differ from that of terrestrial mammals and that their HR-fratio would be higher than in terrestrial species. Literature data for HR （beats/min） in mammals at rest were gathered for 56 terrestrial and 27 aquatic species. In aquatic mammals the allometric curve （HR=191 .M^18; M= body mass, kg） did not differ from that of terrestrial species （HR=212.M^-0.22） and their HR-fratio （on average 32±5） was much higher than in terrestrial species （5±1） （P〈0.0001）. The comparison of these HR allometric curves to those forfpreviously published indicated that the HR-fratio was body size-independent in terrestrial species while it increased significantly with M in aquatic species. The similarity in HR and differences in f between aquatic and terrestrial mammals agree with the possibility that the lowfof aquatic and semi-aquatic mammals may have evolved for a non-respiratory function, namely the regulation of buoyancy at the water surface [Current Zoology 61（4）： 569-577, 2015].展开更多
文摘This paper develops a high time-resolution optimal power generation mix model in its time resolution of 10 minutes on 365 days by linear programming technique. The model allows us to analyse the massive deployment of photovoltaic system and wind power generation in power system explicitly considering those short-term output variation. PV （photovoltaic） and wind output are estimated, employing meteorological database. Simulation results reveal that variable fluctuation derived from a high penetration level of those renewables is controlled by quick load following operation of natural gas combined cycle power plant, pumped-storage hydro power, stationary NAS （sodium and sulfur） battery and the output suppression control of PV and wind. It additionally turns out that the operational configuration of those technologies for the renewable variability differs significantly depending on those renewable output variations in each season and solving the seasonal electricity imbalance as well as the daily imbalance is important if variable renewables are massively deployed.
基金supported by the grants from the National Basic Research Development Program of China(No.2007CB516701,2006CB500704)the National Natural Science Foundation of China(No.30770757)
文摘Objective To construct the recombinant adenovirus vector carrying human growth hormone secretagogue receptor type 1a （GHS-R1a） ,for genetic transfection.Methods The full-length human GHS-R1a gene was obtained by PCR amplification and then cloned into the shuttle plasmid pAdTrack-CMV.The linearized plasmid pAdTrack-CMV-GHS-R1a was co-transformed into Escherichia coli （E.coli） BJ5183 cells along with an adenoviral backbone plasmid pAdEasy1.The HEK293 cells were then infected with adenoviruses.The expression of GHS-R1a was indicated by green fluorescent protein （GFP） ,and confirmed by Reverse Transcription Polymerase Chain Reaction （RT-PCR） and Western blot.Results Enzymatic digestion of pAdGHS-R1a yielded a large fragment （approximately 30 kb） and a small fragment （4.5 kb） ,indicating the success-ful construction of recombinant adenovirus expression vector.Expression of GFP was observed by confocal laser scanning microscopy at 24 h after infection.RT-PCR and Western blot further confirmed that GHS-R1a was efficiently expressed in 293 cells.Conclusion Recombinant adenovirus （AdGHS-R1a） is successfully constructed,and the target gene can be expressed efficiently in 293 cells,which provide a valuable tool for further studying the function of GHS-R1a.
基金Graduate Fund of Beijing Forestry University (183).
文摘Water is a crucial factor influencing eco-environment conservation in the Loess Area in China. Soilmoisture is also an indispensable factor to plant growth because of limited water supply. In this paper,previous studies of soil moisture are summarized from methods and contents. Meanwhile, some problems inthe relevant researches are pointed out and discussed. Some solutions are brought forward.
文摘Aquatic and semi-aquatic mammals, while resting at the water surface or ashore, breathe with a low frequency （f） by comparison to terrestrial mammals of the same body size, the difference increasing the larger the species. Among various interpretations, it was suggested that the low-f breathing is a consequence of the end-inspiratory breath-holding pattern adopted by aquatic mammals to favour buoyancy at the water surface, and evolved to be part of the genetic makeup. If this interpretation was correct it could be expected that, differently from f, the heart rate （HR, beats/min） of aquatic and semi-aquatic mammals at rest would not need to differ from that of terrestrial mammals and that their HR-fratio would be higher than in terrestrial species. Literature data for HR （beats/min） in mammals at rest were gathered for 56 terrestrial and 27 aquatic species. In aquatic mammals the allometric curve （HR=191 .M^18; M= body mass, kg） did not differ from that of terrestrial species （HR=212.M^-0.22） and their HR-fratio （on average 32±5） was much higher than in terrestrial species （5±1） （P〈0.0001）. The comparison of these HR allometric curves to those forfpreviously published indicated that the HR-fratio was body size-independent in terrestrial species while it increased significantly with M in aquatic species. The similarity in HR and differences in f between aquatic and terrestrial mammals agree with the possibility that the lowfof aquatic and semi-aquatic mammals may have evolved for a non-respiratory function, namely the regulation of buoyancy at the water surface [Current Zoology 61（4）： 569-577, 2015].
