Laccases are blue copper oxidases （E.C. 1.10.3.2 benzenediol：oxygen oxidoreductase） that catalyze the one-electron oxidation of phenolics, aromatic amines and other electron-rich substrates with the concomitant red...Laccases are blue copper oxidases （E.C. 1.10.3.2 benzenediol：oxygen oxidoreductase） that catalyze the one-electron oxidation of phenolics, aromatic amines and other electron-rich substrates with the concomitant reduction of 02 to H20. They are currently seen as highly interesting industrial enzymes because of their broad substrate specificity. The Phanerochaete flavido-alba laccase is expressed and secreted as a soluble active enzyme by Aspergillus niger （rLac-LPFA）. rLac-LPFA is easily purified to homogeneity. Metal ions like HgCI2, KC12, FeSO4 and MgSO4 at a concentration of 2 mM have inhibiting effect on recombinant and native laccase, whereas, CuSO4 and MnSO4 moderately increase both enzyme activities. Two potential inhibitors （sodium azide and EDTA） inhibited enzyme activity, whereas, urea and SDS have no effect on enzyme activity. The Km and V,,ax values for recombinant laccase are 0.65 mM and 300 U/mg respectively for 2,6-DMP as substrate.展开更多
文摘Laccases are blue copper oxidases （E.C. 1.10.3.2 benzenediol：oxygen oxidoreductase） that catalyze the one-electron oxidation of phenolics, aromatic amines and other electron-rich substrates with the concomitant reduction of 02 to H20. They are currently seen as highly interesting industrial enzymes because of their broad substrate specificity. The Phanerochaete flavido-alba laccase is expressed and secreted as a soluble active enzyme by Aspergillus niger （rLac-LPFA）. rLac-LPFA is easily purified to homogeneity. Metal ions like HgCI2, KC12, FeSO4 and MgSO4 at a concentration of 2 mM have inhibiting effect on recombinant and native laccase, whereas, CuSO4 and MnSO4 moderately increase both enzyme activities. Two potential inhibitors （sodium azide and EDTA） inhibited enzyme activity, whereas, urea and SDS have no effect on enzyme activity. The Km and V,,ax values for recombinant laccase are 0.65 mM and 300 U/mg respectively for 2,6-DMP as substrate.
