The glucose mass transfer in the biosynthesis of succinic acid with immobilized Actinobacillus succinogenes cells has been comparatively analyzed for a bioreactor with mobile bed vs. a stationary basket bioreactor. Th...The glucose mass transfer in the biosynthesis of succinic acid with immobilized Actinobacillus succinogenes cells has been comparatively analyzed for a bioreactor with mobile bed vs. a stationary basket bioreactor. The process has been considered to occur under substrate and product inhibitory effects. The results indicated that the bioreactor with mobile bed is more efficient for biocatalyst particles with a diameter over 3 mm, while the basket bioreactor is more efficient for smaller biocatalyst particles and basket bed thickness below 5 mm. The performances of both configurations of immobilized A. succinogenes cell beds were found to be superior to the column packed bed bioreactor.展开更多
Exosomes are promising biological biomarkers for monitoring a number of diseases, especially cancers. Here, we developed a double gold nanoparticles （GNPs） conjugates based lateral flow assay （D-LFA） for rapidly a...Exosomes are promising biological biomarkers for monitoring a number of diseases, especially cancers. Here, we developed a double gold nanoparticles （GNPs） conjugates based lateral flow assay （D-LFA） for rapidly and sensitively detecting and molecular profiling of exosomes. Based on these two GNPs conjugates, the signal amplification can be achieved without any additional operation. The antibody on the 1st GNPs conjugate could recognize exosomes and form a sandwich format on the test zone. The 2nd GNPs conjugate was designed to bind to the 1st GNPs conjugate to realize signal amplification. This biosensor enabled visual and quantitative detection of exosomes by the accumulation of GNPs on the test zone and showed a low detection limit of 1.3x10^3 particles/laL, which has been improved 13-fold compared with the normal lateral flow assay. The D-LFA exhibited good sensitivity and reproducibility and has been successfully used for the detection ofexosomes in fetal bovine serum, which proved its potential application in practical diagnostics.展开更多
基金Supported by the Grant PN-II-PT-PCCA-2011-3.1-1268 authorized by The National Council for Scientific Research-Executive Unit for Financing Higher Education,Research,Development and Innovation(CNCS-UEFISCDI)
文摘The glucose mass transfer in the biosynthesis of succinic acid with immobilized Actinobacillus succinogenes cells has been comparatively analyzed for a bioreactor with mobile bed vs. a stationary basket bioreactor. The process has been considered to occur under substrate and product inhibitory effects. The results indicated that the bioreactor with mobile bed is more efficient for biocatalyst particles with a diameter over 3 mm, while the basket bioreactor is more efficient for smaller biocatalyst particles and basket bed thickness below 5 mm. The performances of both configurations of immobilized A. succinogenes cell beds were found to be superior to the column packed bed bioreactor.
基金supported by the National Natural Science Foundation of China (21475009, 21475008)Beijing Municipal Science & Technology Commission (Z161100000116037)
文摘Exosomes are promising biological biomarkers for monitoring a number of diseases, especially cancers. Here, we developed a double gold nanoparticles （GNPs） conjugates based lateral flow assay （D-LFA） for rapidly and sensitively detecting and molecular profiling of exosomes. Based on these two GNPs conjugates, the signal amplification can be achieved without any additional operation. The antibody on the 1st GNPs conjugate could recognize exosomes and form a sandwich format on the test zone. The 2nd GNPs conjugate was designed to bind to the 1st GNPs conjugate to realize signal amplification. This biosensor enabled visual and quantitative detection of exosomes by the accumulation of GNPs on the test zone and showed a low detection limit of 1.3x10^3 particles/laL, which has been improved 13-fold compared with the normal lateral flow assay. The D-LFA exhibited good sensitivity and reproducibility and has been successfully used for the detection ofexosomes in fetal bovine serum, which proved its potential application in practical diagnostics.
