In order to meet the requirement for crystalline growth of O-2-susceptible proteins in space, crystallization conditions on the earth was optimized for the proteins using a simple and suitable device for anaerobic add...In order to meet the requirement for crystalline growth of O-2-susceptible proteins in space, crystallization conditions on the earth was optimized for the proteins using a simple and suitable device for anaerobic addition of the protein samples. Nitrogenase is susceptible to O-2. Delta nifZ MoFe protein from a nifZ deleted strain and MnFe protein from mutant strain UW3 grown on a medium containing Mn were crystallized at the first time in the world using an anaerobic device equipped with plastic bags or using a small simplified box, as a replacement for the cumbersome dry box. And the proteins could be also crystallized far from laboratory by sitting-drop method using a much lighter device. It was equipped with a smaller plastic food bag and a first-aid bag filled with Ar, as a substitute for the cumbersome dry box and the Ar cylinder, respectively. The results showed that the device could meet the requirement for studies on crystal growth of the above anaerobic proteins in space.展开更多
This paper presents the effects of alternative nitrogen management regimes on production of Eucalyptus in tropics and sub-tropics carried out in autumn, 2011. The aim of the study was to review alternative nitrogen ma...This paper presents the effects of alternative nitrogen management regimes on production of Eucalyptus in tropics and sub-tropics carried out in autumn, 2011. The aim of the study was to review alternative nitrogen management regimes that could enhance the positive effects and reduce negative effects of different Eucalyptus species in the perspective of smallholders. Literature review was used to survey the alternative nitrogen management regimes on eucalyptus trees. There was a significant improvement （P ≤ 0.01） in mean annual increment （MAI） as measured at seven years of Eucalyptus tereticornis in the plots planted with only legume, pueraria and stylosanthes, compared to that of the control on altitude 150 m and rainfall of 2,000 mm at the age of seven years, The MAI of Eucalyptus urophylla and Eucalyptus camaldulensis in plots planted with beans and rice shows a significant improvement （P ≤ 0.05） over the control treatment. Only the plots treated with nitrogen fertilizer 550 kg/ha showed significant improvement （P ≤ 0.05） in terms of MAI of Eucalyptus nitens, compared to the control treatment. In general, it can be concluded that as these alternative management regimes enhance productivity of Eucalyptus woodlots, the challenge for the small-scale farmers is on how to manage them sustainably. Therefore, forest policies that take consideration of increasing production of Eucalyptus and managing biodiversity with reference to these alternative management regimes should be developed in tropics and sub-tropics.展开更多
Dear Editor,The nitrogen-fixing symbiotic bacteria, rhizobia are the most important beneficial bacteria in soil, as they form nodules with host legume plants to fix nitrogen to maintain soil fertility and facilitate p...Dear Editor,The nitrogen-fixing symbiotic bacteria, rhizobia are the most important beneficial bacteria in soil, as they form nodules with host legume plants to fix nitrogen to maintain soil fertility and facilitate plant growth. Although these bacteria are critically important to agriculture, bacteriophages of rhizobia (rhizobiophages) are commonly present in soils, rhizosphere,and nodules, which have major influences on the composition and population of rhizobia in soils and affect nodulation and nitrogen fixation. Despite the importance of rhizobiophages,only 23 phage full genomes have been sequenced.展开更多
文摘In order to meet the requirement for crystalline growth of O-2-susceptible proteins in space, crystallization conditions on the earth was optimized for the proteins using a simple and suitable device for anaerobic addition of the protein samples. Nitrogenase is susceptible to O-2. Delta nifZ MoFe protein from a nifZ deleted strain and MnFe protein from mutant strain UW3 grown on a medium containing Mn were crystallized at the first time in the world using an anaerobic device equipped with plastic bags or using a small simplified box, as a replacement for the cumbersome dry box. And the proteins could be also crystallized far from laboratory by sitting-drop method using a much lighter device. It was equipped with a smaller plastic food bag and a first-aid bag filled with Ar, as a substitute for the cumbersome dry box and the Ar cylinder, respectively. The results showed that the device could meet the requirement for studies on crystal growth of the above anaerobic proteins in space.
文摘This paper presents the effects of alternative nitrogen management regimes on production of Eucalyptus in tropics and sub-tropics carried out in autumn, 2011. The aim of the study was to review alternative nitrogen management regimes that could enhance the positive effects and reduce negative effects of different Eucalyptus species in the perspective of smallholders. Literature review was used to survey the alternative nitrogen management regimes on eucalyptus trees. There was a significant improvement （P ≤ 0.01） in mean annual increment （MAI） as measured at seven years of Eucalyptus tereticornis in the plots planted with only legume, pueraria and stylosanthes, compared to that of the control on altitude 150 m and rainfall of 2,000 mm at the age of seven years, The MAI of Eucalyptus urophylla and Eucalyptus camaldulensis in plots planted with beans and rice shows a significant improvement （P ≤ 0.05） over the control treatment. Only the plots treated with nitrogen fertilizer 550 kg/ha showed significant improvement （P ≤ 0.05） in terms of MAI of Eucalyptus nitens, compared to the control treatment. In general, it can be concluded that as these alternative management regimes enhance productivity of Eucalyptus woodlots, the challenge for the small-scale farmers is on how to manage them sustainably. Therefore, forest policies that take consideration of increasing production of Eucalyptus and managing biodiversity with reference to these alternative management regimes should be developed in tropics and sub-tropics.
基金supported by the Strategic Priority Research Program of Chinese Academy of Sciences (XDB15010103)National Natural Science Foundation of China (41301259, 41671251)Youth Innovation Promotion Association CAS
文摘Dear Editor,The nitrogen-fixing symbiotic bacteria, rhizobia are the most important beneficial bacteria in soil, as they form nodules with host legume plants to fix nitrogen to maintain soil fertility and facilitate plant growth. Although these bacteria are critically important to agriculture, bacteriophages of rhizobia (rhizobiophages) are commonly present in soils, rhizosphere,and nodules, which have major influences on the composition and population of rhizobia in soils and affect nodulation and nitrogen fixation. Despite the importance of rhizobiophages,only 23 phage full genomes have been sequenced.
