Nutrition is an important factor for the growth and development of plants. Among the main nutritional elements, there are nitrogen (N) and calcium (Ca). The N comes from two forms of inorganic ions, ammonium (NH...Nutrition is an important factor for the growth and development of plants. Among the main nutritional elements, there are nitrogen (N) and calcium (Ca). The N comes from two forms of inorganic ions, ammonium (NH4+) and nitrate (NO3) whose functions in the plant are the increase in leaf area and improving the succulence of many crops, among other essential physiological processes. Both the deficit and excess NO3- have a negative impact on plants increasing susceptibility to insect pests and pathogens, while Ca as divalent ion absorbed Ca2+ plays an important role in control of physiological disorders and the resistance of plants to diseases to increase the mechanical strength. The N and Ca have a close relationship in the nutritional role of the plant, because the Ca absorption acting nitrates, so that a proper balance affects plants better growth and higher strength.展开更多
Photosynthetic production is a major determinant of final yield in crop plants. A simulation model was developed for canopy photosynthesis and dry matter accumulation in oilseed rape (Brassica napus L.) based on the e...Photosynthetic production is a major determinant of final yield in crop plants. A simulation model was developed for canopy photosynthesis and dry matter accumulation in oilseed rape (Brassica napus L.) based on the ecophysiological processes and using a three-layer radiation balance scheme for calculating the radiation interception and absorption by the layers of flowers, pods, and leaves within the canopy. Gaussian integration method was used to calculate photosynthesis of the pod and leaf layers, and the daily total canopy photosynthesis was determined by the sum of photosynthesis from the two layers of green organs. The effects of physiological age, temperature, nitrogen, and water deficit on maximum photosynthetic rate were quantified. Maintenance and growth respiration were estimated to determine net photosynthetic production. Partition index of the shoot in relation to physiological development time was used to calculate shoot dry matter from plant biomass and shoot biomass loss because of freezing was quantified by temperature effectiveness. Testing of the model for dynamic dry matter accumulation through field experiments of different genotypes, sowing dates, and nitrogen levels showed good fit between the observed and simulated data, with an average root mean square error of 10.9% for shoot dry matter. Thus, the present model appears to be reliable for the prediction of photosynthetic production in oilseed rape.展开更多
The article considers the econophysical analysis of the relationship between monopoly and competition by using the methods, terms of physics. And it was investigated the philosophy of progress. It has been shown that ...The article considers the econophysical analysis of the relationship between monopoly and competition by using the methods, terms of physics. And it was investigated the philosophy of progress. It has been shown that in the transition from absolute monopoly to imperfect monopoly, the system becomes more complex, and its output characteristics depend on time, because monopoly is a natural, competitive is derivative process. Competition is created as a result of the interaction of at least two ~monopoly firms" through the ~market field" that they create to increase the production which is necessary of non-linear products over time. To do this, it is sufficient to have a multitude of firms interacting with each other under the influence force of ~market field". To create the necessary conditions, it is sufficient to have a high level of university education and a legal field for competition and unbreakable antitrust legislation. By acquiring technology and creating conditions for competition in the market, it is possible to achieve progress even without having a strong science. The term ~progress" has received a new content and is defined as the value of a numerically equal increase in the rate of production per unit time or production per squared time. It has been shown that the relationship between monopoly and competition is very simple and there is no contradiction between them. Initially, the market is born as a monopoly, and then analogical firms were created, competition between firms begins展开更多
Deep-sea environment,characterized by high pressures,extremely high/low temperatures,limited photosynthesis-generated organic matter,darkness,and high levels of corrosion,is home to flourishing special ecosystems in t...Deep-sea environment,characterized by high pressures,extremely high/low temperatures,limited photosynthesis-generated organic matter,darkness,and high levels of corrosion,is home to flourishing special ecosystems in the world.Here,we illustrate how the deep-sea equipment offers insights into the study of life in the deep sea based on the work in the past five decades.We first describe how organisms in the deep sea are studied,even though it is highly difficult to get access to such extreme environments.We then explain the role of deep-sea technologies in advancing research on the evolution of organisms in hydrothermal vents,cold seeps,seamounts,oceanic trenches,and whale falls from the following perspectives:biological diversity,mechanisms of environmental adaptation,biological evolution,and ecosystem connectivity.Finally,to better understand the function and service of deep-sea organisms,and further conserve the special creatures under anthropologic activity and climate change,we highlight the importance of innovative deep-sea technologies to promote cutting-edge research on deep-sea organisms,and note the remaining challenges and developing directions for deep-sea equipment.展开更多
Membrane proteins are involved in various critical biological processes,and studying membrane proteins represents a major challenge in protein biochemistry.As shown by both structural and functional studies,the membra...Membrane proteins are involved in various critical biological processes,and studying membrane proteins represents a major challenge in protein biochemistry.As shown by both structural and functional studies,the membrane environment plays an essential role for membrane proteins.In vitro studies are reliant on the successful reconstitution of membrane proteins.This review describes the interaction between detergents and lipids that aids the understanding of the reconstitution processes.Then the techniques of detergent removal and a few useful techniques to refine the formed proteoliposomes are reviewed.Finally the applications of reconstitution techniques to study membrane proteins involved in Ca2+ signaling are summarized.展开更多
Most E26 transformation-specific (ETS) transcription factors are involved in the pathogenesis and progression of cancer. This is in part due to the roles of ETS transcription factors in basic biological processes su...Most E26 transformation-specific (ETS) transcription factors are involved in the pathogenesis and progression of cancer. This is in part due to the roles of ETS transcription factors in basic biological processes such as growth, proliferation, and differentiation, and also because of their regulatory functions that have physiological relevance in tumorigenesis, immunity, and basal cellular homoeostasis. A member of the E74-1ike factor (ELF) subfamily of the ETS transcription factor family--myeloid elf-l-Uke factor (MEF), designated as ELF4~has been shown to be critically involved in immune response and signalling, osteogenesis, adipo- genesis, cancer, and stem cell quiescence. ELF4 carries out these functions as a transcriptional activator or through interactions with its partner proteins. Mutations in ELF4 cause aberrant interactions and induce downstream processes that may lead to dis- eased cells. Knowing how ELF4 impinges on certain cellular processes and how it is regulated in the cells can lead to a better understanding of the physiological and pathological consequences of modulated ELF4 activity.展开更多
microRNAs regulate diverse biological processes such as development and aging by promoting degradation or inhibiting translation of their target mRNAs. In this study, we have found that the miR-58 family microRNAs reg...microRNAs regulate diverse biological processes such as development and aging by promoting degradation or inhibiting translation of their target mRNAs. In this study, we have found that the miR-58 family microRNAs regulate lifespan in C.elegans. Intriguingly, members of the miR-58 family affect lifespan differently, sometimes in opposite directions, and have complex genetic interactions. The abundances of the miR-58 family miRNAs are up-regulated in the long-lived daf-2 mutant in a daf-16-dependent manner, indicating that these miRNAs are effectors of insulin signaling in C. elegans. We also found that miR-58 is regulated by insulin signaling and partially required for the lifespan extension mediated by reduced insulin signaling,germline ablation, dietary restriction, and mild mitochondrial dysfunction. We further identified the daf-21, ins-1, and isw-1 mRNAs as endogenous targets of miR-58. Our study shows that miRNAs function in multiple lifespan extension mechanisms,and that the seed sequence is not the dominant factor defining the role of a miRNA in lifespan regulation.展开更多
Mammals and other complex organisms can transcribe an abundance of long non-coding RNAs(lncRNAs)that fulfill a wide variety of regulatory roles in many biological processes.These roles,including as scaffolds and as gu...Mammals and other complex organisms can transcribe an abundance of long non-coding RNAs(lncRNAs)that fulfill a wide variety of regulatory roles in many biological processes.These roles,including as scaffolds and as guides for protein-coding genes,mainly depend on the structure and expression level of lncRNAs.In this review,we focus on the current methods for analyzing lncRNA structure and expression,which is basic but necessary information for in-depth,large-scale analysis of lncRNA functions.展开更多
文摘Nutrition is an important factor for the growth and development of plants. Among the main nutritional elements, there are nitrogen (N) and calcium (Ca). The N comes from two forms of inorganic ions, ammonium (NH4+) and nitrate (NO3) whose functions in the plant are the increase in leaf area and improving the succulence of many crops, among other essential physiological processes. Both the deficit and excess NO3- have a negative impact on plants increasing susceptibility to insect pests and pathogens, while Ca as divalent ion absorbed Ca2+ plays an important role in control of physiological disorders and the resistance of plants to diseases to increase the mechanical strength. The N and Ca have a close relationship in the nutritional role of the plant, because the Ca absorption acting nitrates, so that a proper balance affects plants better growth and higher strength.
基金Project supported by the National High Technology Research and Development Program (863 Program) of China(No. 2006AA10A303)the Post-Doctoral Program of Jiangsu Province, China (No. 0602027C)
文摘Photosynthetic production is a major determinant of final yield in crop plants. A simulation model was developed for canopy photosynthesis and dry matter accumulation in oilseed rape (Brassica napus L.) based on the ecophysiological processes and using a three-layer radiation balance scheme for calculating the radiation interception and absorption by the layers of flowers, pods, and leaves within the canopy. Gaussian integration method was used to calculate photosynthesis of the pod and leaf layers, and the daily total canopy photosynthesis was determined by the sum of photosynthesis from the two layers of green organs. The effects of physiological age, temperature, nitrogen, and water deficit on maximum photosynthetic rate were quantified. Maintenance and growth respiration were estimated to determine net photosynthetic production. Partition index of the shoot in relation to physiological development time was used to calculate shoot dry matter from plant biomass and shoot biomass loss because of freezing was quantified by temperature effectiveness. Testing of the model for dynamic dry matter accumulation through field experiments of different genotypes, sowing dates, and nitrogen levels showed good fit between the observed and simulated data, with an average root mean square error of 10.9% for shoot dry matter. Thus, the present model appears to be reliable for the prediction of photosynthetic production in oilseed rape.
文摘The article considers the econophysical analysis of the relationship between monopoly and competition by using the methods, terms of physics. And it was investigated the philosophy of progress. It has been shown that in the transition from absolute monopoly to imperfect monopoly, the system becomes more complex, and its output characteristics depend on time, because monopoly is a natural, competitive is derivative process. Competition is created as a result of the interaction of at least two ~monopoly firms" through the ~market field" that they create to increase the production which is necessary of non-linear products over time. To do this, it is sufficient to have a multitude of firms interacting with each other under the influence force of ~market field". To create the necessary conditions, it is sufficient to have a high level of university education and a legal field for competition and unbreakable antitrust legislation. By acquiring technology and creating conditions for competition in the market, it is possible to achieve progress even without having a strong science. The term ~progress" has received a new content and is defined as the value of a numerically equal increase in the rate of production per unit time or production per squared time. It has been shown that the relationship between monopoly and competition is very simple and there is no contradiction between them. Initially, the market is born as a monopoly, and then analogical firms were created, competition between firms begins
基金supported by the National Natural Science Foundation of China(42022046)the National Key Research and Development Program(2021YFF0502300)+1 种基金the Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou)(GML2019ZD0401 and GML2019ZD0403)Guangdong Natural Resources Foundation(GDNRC[2022]45)。
文摘Deep-sea environment,characterized by high pressures,extremely high/low temperatures,limited photosynthesis-generated organic matter,darkness,and high levels of corrosion,is home to flourishing special ecosystems in the world.Here,we illustrate how the deep-sea equipment offers insights into the study of life in the deep sea based on the work in the past five decades.We first describe how organisms in the deep sea are studied,even though it is highly difficult to get access to such extreme environments.We then explain the role of deep-sea technologies in advancing research on the evolution of organisms in hydrothermal vents,cold seeps,seamounts,oceanic trenches,and whale falls from the following perspectives:biological diversity,mechanisms of environmental adaptation,biological evolution,and ecosystem connectivity.Finally,to better understand the function and service of deep-sea organisms,and further conserve the special creatures under anthropologic activity and climate change,we highlight the importance of innovative deep-sea technologies to promote cutting-edge research on deep-sea organisms,and note the remaining challenges and developing directions for deep-sea equipment.
基金supported by US National Institutes of Health(R01GM096458)to Wang LiGuo
文摘Membrane proteins are involved in various critical biological processes,and studying membrane proteins represents a major challenge in protein biochemistry.As shown by both structural and functional studies,the membrane environment plays an essential role for membrane proteins.In vitro studies are reliant on the successful reconstitution of membrane proteins.This review describes the interaction between detergents and lipids that aids the understanding of the reconstitution processes.Then the techniques of detergent removal and a few useful techniques to refine the formed proteoliposomes are reviewed.Finally the applications of reconstitution techniques to study membrane proteins involved in Ca2+ signaling are summarized.
文摘Most E26 transformation-specific (ETS) transcription factors are involved in the pathogenesis and progression of cancer. This is in part due to the roles of ETS transcription factors in basic biological processes such as growth, proliferation, and differentiation, and also because of their regulatory functions that have physiological relevance in tumorigenesis, immunity, and basal cellular homoeostasis. A member of the E74-1ike factor (ELF) subfamily of the ETS transcription factor family--myeloid elf-l-Uke factor (MEF), designated as ELF4~has been shown to be critically involved in immune response and signalling, osteogenesis, adipo- genesis, cancer, and stem cell quiescence. ELF4 carries out these functions as a transcriptional activator or through interactions with its partner proteins. Mutations in ELF4 cause aberrant interactions and induce downstream processes that may lead to dis- eased cells. Knowing how ELF4 impinges on certain cellular processes and how it is regulated in the cells can lead to a better understanding of the physiological and pathological consequences of modulated ELF4 activity.
基金supported by the NIH Office of Infrastructure Programs (P40 OD010440), for providing worm strainssupported by Beijing Municipal Science and Technology Commission (a fund for cultivation and development of innovation base) and the Ministry of Science and Technology of China (2014CB84980001)
文摘microRNAs regulate diverse biological processes such as development and aging by promoting degradation or inhibiting translation of their target mRNAs. In this study, we have found that the miR-58 family microRNAs regulate lifespan in C.elegans. Intriguingly, members of the miR-58 family affect lifespan differently, sometimes in opposite directions, and have complex genetic interactions. The abundances of the miR-58 family miRNAs are up-regulated in the long-lived daf-2 mutant in a daf-16-dependent manner, indicating that these miRNAs are effectors of insulin signaling in C. elegans. We also found that miR-58 is regulated by insulin signaling and partially required for the lifespan extension mediated by reduced insulin signaling,germline ablation, dietary restriction, and mild mitochondrial dysfunction. We further identified the daf-21, ins-1, and isw-1 mRNAs as endogenous targets of miR-58. Our study shows that miRNAs function in multiple lifespan extension mechanisms,and that the seed sequence is not the dominant factor defining the role of a miRNA in lifespan regulation.
基金supported by the National High Technology Research and Development Program of China(2012AA020402)National Natural Science Foundation of China(11074084,30970558)
文摘Mammals and other complex organisms can transcribe an abundance of long non-coding RNAs(lncRNAs)that fulfill a wide variety of regulatory roles in many biological processes.These roles,including as scaffolds and as guides for protein-coding genes,mainly depend on the structure and expression level of lncRNAs.In this review,we focus on the current methods for analyzing lncRNA structure and expression,which is basic but necessary information for in-depth,large-scale analysis of lncRNA functions.