[Objective]The research aimed to study the calling and mating behavior of Malacosoma rectifascia Lajonquère and provide theoretical basis for utilization of sex pheromone to prevent and control M.rectifascia. [Me...[Objective]The research aimed to study the calling and mating behavior of Malacosoma rectifascia Lajonquère and provide theoretical basis for utilization of sex pheromone to prevent and control M.rectifascia. [Method]Through indoor and outdoor observation,effects of M.rectifascia mating behavior occurrence process,calling period,different sex ratio and population density on mating rate were studied. [Result]The sex ratio of M.rectifascia in nature was 1∶1.2,while in lab was 1∶1.8; the calling peak took place from 19：00 to 21：00;different sex ratio and population density had significant effects on mating behavior,and mating rate would be reduced if the population density was higher; both male and female M.rectifascia mate only once throughout their life. [Conclusion]Controlling M.rectifascia with sex pheromone has good application prospects.展开更多
To extract vegetation pigment concentration and physiological status has been studied in two test areas covered with swamp and flourish vegetation using pushbroom hyperspectral imager (PHI) data which flied in Septemb...To extract vegetation pigment concentration and physiological status has been studied in two test areas covered with swamp and flourish vegetation using pushbroom hyperspectral imager (PHI) data which flied in September of 2000 at Daxing'anling district of Heilongjiang Province, China. The ratio analysis of reflectance spectra (RARS) indices, which were put forward by Chappelle et al (1992), are chosen in this paper owing to their effect and simpleness against both comparison with various methods and techniques for exploration of pigment concentration and characteristics of PHI data. The correlation coefficients between RARS indices and pigment concentration of vegetation were up to 0.8. The new RARS indices modes are established in the two test areas using both PHI data and spectra of different vegetations measured in the field. The indices' parameter images of chlorophyll a (Chl a), chlorophyll b (Chl b) and carotenoids (Cars) of the test areas covered with swamp and flourish vegetation are acquired by the new RARS indices modes. Furthermore, the regional concentration of Chl a and Chl b are extracted and quantified using regression equations between RARS indices and pigment concentrations, which were built by Blackburn (1998). The results showed the physiological status and variety clearly, and are in good agreement with the distribution of vegetation in the field.展开更多
Artemisinin, a sesquiterpene lactone endoperoxide derived from Artemisia annua L, forms the basis of the most important medicines for treating malaria in use today. In the study, a total of 41 full genes coded cytochr...Artemisinin, a sesquiterpene lactone endoperoxide derived from Artemisia annua L, forms the basis of the most important medicines for treating malaria in use today. In the study, a total of 41 full genes coded cytochrome P450 monooxygenases were identified from NCBI. These genes were classified into 11 families in 3 gene clans according to sequence similarity. One of the first interesting features in sequence alignment was that the CYP82, CYP83 and CYP716 families specific in Arabidopsis genome were present in Artemisia, and the CYP92 specific in rice was also present in Artemisia. The physical and chemical properties and structure characteristics of the identified P450s were studied. The results showed their secondary structures were very similar and their senior structures mainly contained α-helix.展开更多
Ethylene plays an extensive role in plant growth and development.. 1-aminocyclopropane-1-carboxylate （ACC） oxidase （ACO） is the key enzyme in ethylene biosynthesis. In this study, a 354 g DNA and a 213 bp cDNA bas...Ethylene plays an extensive role in plant growth and development.. 1-aminocyclopropane-1-carboxylate （ACC） oxidase （ACO） is the key enzyme in ethylene biosynthesis. In this study, a 354 g DNA and a 213 bp cDNA base pair （bp） candidate fragment was amplified from pepper with primers derived from the ACO sequence （AJ011109） reported by Ernesto. The putative new gene was analyzed by bioinformatics tools.展开更多
Cytokinin plays a very important role in plants growth and development,and CRE1 has been identified as a cytokinin receptor.However,the biological function of CRE1 in cotton remains unclear.In this paper,GhCRE1 gene w...Cytokinin plays a very important role in plants growth and development,and CRE1 has been identified as a cytokinin receptor.However,the biological function of CRE1 in cotton remains unclear.In this paper,GhCRE1 gene was cloned and its sequence was analyzed by bioinformatics.The results revealed that GhCRE1 had 11 exons and 10 introns,and its molecular weight,theoretical isoelectric point(pI)and number of amino acids differed from those of the homologous gene in Theobroma cacao and Arabidopsis thaliana,with three different protein domains.15 unidentified proteins were found in potential interaction with CRE1 protein and 2 phosphorylation sites on CRE1 protein sequence were predicted by mutiple bioinformatics websites.Additionally,8 cis-acting regulatory elements were detected on CRE1 promoter sequence,and found related to light signal and hormone.These results were conducive to unfolding the function of GhCRE1 in upland cotton.展开更多
To identify novel genes in castration-resistant prostate cancer(CRPC),we downloaded three microarray datasets containing CRPC and primary prostate cancer in Gene Expression Omnibus(GEO).R packages affy and limma were ...To identify novel genes in castration-resistant prostate cancer(CRPC),we downloaded three microarray datasets containing CRPC and primary prostate cancer in Gene Expression Omnibus(GEO).R packages affy and limma were performed to identify differentially expressed genes(DEGs)between primary prostate cancer and CRPC.After that,we performed functional enrichment analysis including gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)pathway.In addition,protein–protein interaction(PPI)analysis was used to search for hub genes.Finally,to validate the significance of these genes,we performed survival analysis.As a result,we identified 53 upregulated genes and 58 downregulated genes that changed in at least two datasets.Functional enrichment analysis showed significant changes in the positive regulation of osteoblast differentiation pathway and aldosteroneregulated sodium reabsorption pathway.PPI network identified hub genes like cortactin-binding protein 2(CTTNBP2),Rho family guanosine triphosphatase(GTPase)3(RND3),protein tyrosine phosphatase receptor-type R(PTPRR),Jagged1(JAG1),and lumican(LUM).Based on PPI network analysis and functional enrichment analysis,we identified two genes(PTPRR and JAG1)as key genes.Further survival analysis indicated a relationship between high expression of the two genes and poor prognosis of prostate cancer.In conclusion,PTPRR and JAG1 are key genes in the CRPC,which may serve as promising biomarkers of diagnosis and prognosis of CRPC.展开更多
基金Supported by Shanxi Agricultural University Technology Innovation Fund Projects (2005011, 2004003)~~
文摘[Objective]The research aimed to study the calling and mating behavior of Malacosoma rectifascia Lajonquère and provide theoretical basis for utilization of sex pheromone to prevent and control M.rectifascia. [Method]Through indoor and outdoor observation,effects of M.rectifascia mating behavior occurrence process,calling period,different sex ratio and population density on mating rate were studied. [Result]The sex ratio of M.rectifascia in nature was 1∶1.2,while in lab was 1∶1.8; the calling peak took place from 19：00 to 21：00;different sex ratio and population density had significant effects on mating behavior,and mating rate would be reduced if the population density was higher; both male and female M.rectifascia mate only once throughout their life. [Conclusion]Controlling M.rectifascia with sex pheromone has good application prospects.
文摘To extract vegetation pigment concentration and physiological status has been studied in two test areas covered with swamp and flourish vegetation using pushbroom hyperspectral imager (PHI) data which flied in September of 2000 at Daxing'anling district of Heilongjiang Province, China. The ratio analysis of reflectance spectra (RARS) indices, which were put forward by Chappelle et al (1992), are chosen in this paper owing to their effect and simpleness against both comparison with various methods and techniques for exploration of pigment concentration and characteristics of PHI data. The correlation coefficients between RARS indices and pigment concentration of vegetation were up to 0.8. The new RARS indices modes are established in the two test areas using both PHI data and spectra of different vegetations measured in the field. The indices' parameter images of chlorophyll a (Chl a), chlorophyll b (Chl b) and carotenoids (Cars) of the test areas covered with swamp and flourish vegetation are acquired by the new RARS indices modes. Furthermore, the regional concentration of Chl a and Chl b are extracted and quantified using regression equations between RARS indices and pigment concentrations, which were built by Blackburn (1998). The results showed the physiological status and variety clearly, and are in good agreement with the distribution of vegetation in the field.
文摘Artemisinin, a sesquiterpene lactone endoperoxide derived from Artemisia annua L, forms the basis of the most important medicines for treating malaria in use today. In the study, a total of 41 full genes coded cytochrome P450 monooxygenases were identified from NCBI. These genes were classified into 11 families in 3 gene clans according to sequence similarity. One of the first interesting features in sequence alignment was that the CYP82, CYP83 and CYP716 families specific in Arabidopsis genome were present in Artemisia, and the CYP92 specific in rice was also present in Artemisia. The physical and chemical properties and structure characteristics of the identified P450s were studied. The results showed their secondary structures were very similar and their senior structures mainly contained α-helix.
文摘Ethylene plays an extensive role in plant growth and development.. 1-aminocyclopropane-1-carboxylate （ACC） oxidase （ACO） is the key enzyme in ethylene biosynthesis. In this study, a 354 g DNA and a 213 bp cDNA base pair （bp） candidate fragment was amplified from pepper with primers derived from the ACO sequence （AJ011109） reported by Ernesto. The putative new gene was analyzed by bioinformatics tools.
文摘Cytokinin plays a very important role in plants growth and development,and CRE1 has been identified as a cytokinin receptor.However,the biological function of CRE1 in cotton remains unclear.In this paper,GhCRE1 gene was cloned and its sequence was analyzed by bioinformatics.The results revealed that GhCRE1 had 11 exons and 10 introns,and its molecular weight,theoretical isoelectric point(pI)and number of amino acids differed from those of the homologous gene in Theobroma cacao and Arabidopsis thaliana,with three different protein domains.15 unidentified proteins were found in potential interaction with CRE1 protein and 2 phosphorylation sites on CRE1 protein sequence were predicted by mutiple bioinformatics websites.Additionally,8 cis-acting regulatory elements were detected on CRE1 promoter sequence,and found related to light signal and hormone.These results were conducive to unfolding the function of GhCRE1 in upland cotton.
文摘To identify novel genes in castration-resistant prostate cancer(CRPC),we downloaded three microarray datasets containing CRPC and primary prostate cancer in Gene Expression Omnibus(GEO).R packages affy and limma were performed to identify differentially expressed genes(DEGs)between primary prostate cancer and CRPC.After that,we performed functional enrichment analysis including gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)pathway.In addition,protein–protein interaction(PPI)analysis was used to search for hub genes.Finally,to validate the significance of these genes,we performed survival analysis.As a result,we identified 53 upregulated genes and 58 downregulated genes that changed in at least two datasets.Functional enrichment analysis showed significant changes in the positive regulation of osteoblast differentiation pathway and aldosteroneregulated sodium reabsorption pathway.PPI network identified hub genes like cortactin-binding protein 2(CTTNBP2),Rho family guanosine triphosphatase(GTPase)3(RND3),protein tyrosine phosphatase receptor-type R(PTPRR),Jagged1(JAG1),and lumican(LUM).Based on PPI network analysis and functional enrichment analysis,we identified two genes(PTPRR and JAG1)as key genes.Further survival analysis indicated a relationship between high expression of the two genes and poor prognosis of prostate cancer.In conclusion,PTPRR and JAG1 are key genes in the CRPC,which may serve as promising biomarkers of diagnosis and prognosis of CRPC.
