Algal allelopathy is a manifold ecological/physiological phenomenon that is focused on chemical interactions and autotoxicity. We investigated the allelopathic interactions between Karenia mikimotoi and Dunaliella sal...Algal allelopathy is a manifold ecological/physiological phenomenon that is focused on chemical interactions and autotoxicity. We investigated the allelopathic interactions between Karenia mikimotoi and Dunaliella salina in laboratory cultures based on different temperature (15℃, 20℃, and 25℃) and lighting (40,80, and 160 umol/(m2·s)) conditions. The growth of D. salina in bi-algae culture (1:1 size/density) was significantly restrained. The results of cell-free filtrate culture indicate that direct cell-to- cell contact was not necessary in interspecific competition. Further experimental results demonstrated that allelochemicals released from K. miMmotoi were markedly influenced by both temperature (P=0.013) and irradiance (P=0.003), resulting in different growth characteristics olD. salina in filtrate mediums. Compared with the plateau period, K. mikimotoi exudates in the exponential phase had a stronger short-term inhibition effect on D. salina in normal conditions. A clear concentration-dependent relationship was observed in the effect of allelochemicals released from K. mikimotoi with low-promoting and high-repressing effects on D. Salina in a short time-scale. In addition, allelopathic substances remain stable and effective under high temperature and pressure stress. Many flocculent sediments adhering with D. salina cells were observed in all filtrate mediums, while the quantity and color depended on the original culture conditions.展开更多
A new biomass-gasification power plant, of medium-size downdraft type, is presented and discussed in its design features and performance characteristics. Its configuration and overall dimensions, initially conceived f...A new biomass-gasification power plant, of medium-size downdraft type, is presented and discussed in its design features and performance characteristics. Its configuration and overall dimensions, initially conceived for 800 kW, were recently re-tuned, from a functional point of view and on the base of a parallel theoretical analysis, by decreasing to about 400 kW the former nominal power level. This provision, jointly with the basic design choice of adopting a long and amply dimensioned inlet-biomass thermal pretreatment section, turned out quite effective in achieving high gasification temperatures and a low-tar content in the produced gas at fuel-to-air ratios well below the usually imposed ones, to the advantage of the heat value of the product-gas. The paper discusses the numerical analysis results which helped to properly re-adjust the operational parameters of the gasifier and then presents the experimental performance data of the overall power plant including biomass consumption, gasification temperatures, gas production, composition and pollutants content, cold-gas conversion efficiency and global electric efficiency. Special care is devoted to investigating the issue of a significant production of carbon-containing particulate matter in the product gas, which turns out made up of char and fixed carbon much more than of tar species.展开更多
In order to define the combined effects of spinosad and plant allelochemicals on Helicoverpa armigera(Lepidoptera:Noctuvidae), the research fed them on artificial diets with different doses of spinosad, gossypol, quer...In order to define the combined effects of spinosad and plant allelochemicals on Helicoverpa armigera(Lepidoptera:Noctuvidae), the research fed them on artificial diets with different doses of spinosad, gossypol, quercetin and tannicacid, and the changes of pupal and imaginal biological characteristics were observed and recorded. The results showed that spinosad at sublethal concentrations decreased pupation ratio and pupal weight, prolonged prepupal and pupal periods, and decreased emergence ratio, fecundity and longevity of adults. Combined with plant allelochemicals, spinosad significantly prolonged pupal period and shorten adult longevity. Other life-cycle parameters such as pupation ratio, pupal weights, emergence ratio, eggs laid per female and hatch ratio did not change significantly. The results clearly indicated that plant allelochemicals did not change spinosad efficiency on H. armigera, but the combination of spinosad and plant allelochemicals might affect pest population dynamics significantly by changing its development.展开更多
The present study achieves the biochemistry partial characterization of the chitinolytic extract produced by an endophytic Streptomyces sp. strain (A8). This extract was also tested against Anthonomus grandis, the c...The present study achieves the biochemistry partial characterization of the chitinolytic extract produced by an endophytic Streptomyces sp. strain (A8). This extract was also tested against Anthonomus grandis, the cotton boll weevil aiming its control. The chitinase crude extract from the A8 strain was cultured for five days in a minimum liquid media supplemented with chitin. The extract was partially characterized by standard methods. The chitinolytic extract had an optimum temperature of 66 "C and an optimum pH between 4 to 9 (around 80% of relative activity). We also characterized the temperature and pH stability and measured the effects of enzyme inhibitors. The filtered chitinolytic extract was added to an artificial boll weevil diet. Boll weevil development from the egg stage to the adult stage was prolonged, and the percentage of adults that emerged was approximately 66% less than on control diet. This study showed that the.larval development of A. grandis was inhibited by the presence of characterized chitinolytic extract in artificial diet. This work provides an experimental basis for using the chitinase from an endophytic bacterium Streptomyces sp. as a biocontrol alternativeto controlling the plant pest A. grandis.展开更多
基金Supported by the State Key Laboratory of Satellite Ocean Environment Dynamics(Second Institute of Oceanography,SOA)(No.SOED1418)the Public Science and Technology Research Funds Projects of Ocean(No.201305027)+1 种基金the National Natural Science Foundation of China(No.91128212)the Research Fund for the Doctoral Program of Higher Education of China(No.20110132120025)
文摘Algal allelopathy is a manifold ecological/physiological phenomenon that is focused on chemical interactions and autotoxicity. We investigated the allelopathic interactions between Karenia mikimotoi and Dunaliella salina in laboratory cultures based on different temperature (15℃, 20℃, and 25℃) and lighting (40,80, and 160 umol/(m2·s)) conditions. The growth of D. salina in bi-algae culture (1:1 size/density) was significantly restrained. The results of cell-free filtrate culture indicate that direct cell-to- cell contact was not necessary in interspecific competition. Further experimental results demonstrated that allelochemicals released from K. miMmotoi were markedly influenced by both temperature (P=0.013) and irradiance (P=0.003), resulting in different growth characteristics olD. salina in filtrate mediums. Compared with the plateau period, K. mikimotoi exudates in the exponential phase had a stronger short-term inhibition effect on D. salina in normal conditions. A clear concentration-dependent relationship was observed in the effect of allelochemicals released from K. mikimotoi with low-promoting and high-repressing effects on D. Salina in a short time-scale. In addition, allelopathic substances remain stable and effective under high temperature and pressure stress. Many flocculent sediments adhering with D. salina cells were observed in all filtrate mediums, while the quantity and color depended on the original culture conditions.
文摘A new biomass-gasification power plant, of medium-size downdraft type, is presented and discussed in its design features and performance characteristics. Its configuration and overall dimensions, initially conceived for 800 kW, were recently re-tuned, from a functional point of view and on the base of a parallel theoretical analysis, by decreasing to about 400 kW the former nominal power level. This provision, jointly with the basic design choice of adopting a long and amply dimensioned inlet-biomass thermal pretreatment section, turned out quite effective in achieving high gasification temperatures and a low-tar content in the produced gas at fuel-to-air ratios well below the usually imposed ones, to the advantage of the heat value of the product-gas. The paper discusses the numerical analysis results which helped to properly re-adjust the operational parameters of the gasifier and then presents the experimental performance data of the overall power plant including biomass consumption, gasification temperatures, gas production, composition and pollutants content, cold-gas conversion efficiency and global electric efficiency. Special care is devoted to investigating the issue of a significant production of carbon-containing particulate matter in the product gas, which turns out made up of char and fixed carbon much more than of tar species.
基金Supported by Technology Innovation Team of Pesticide Innovation and High efficiency Application of Pesticide(13C1109)Discipline Construction of Anhui Agricultural Sciences(15A1119)Major Science and Technology of Anhui Province(15czz03132)
文摘In order to define the combined effects of spinosad and plant allelochemicals on Helicoverpa armigera(Lepidoptera:Noctuvidae), the research fed them on artificial diets with different doses of spinosad, gossypol, quercetin and tannicacid, and the changes of pupal and imaginal biological characteristics were observed and recorded. The results showed that spinosad at sublethal concentrations decreased pupation ratio and pupal weight, prolonged prepupal and pupal periods, and decreased emergence ratio, fecundity and longevity of adults. Combined with plant allelochemicals, spinosad significantly prolonged pupal period and shorten adult longevity. Other life-cycle parameters such as pupation ratio, pupal weights, emergence ratio, eggs laid per female and hatch ratio did not change significantly. The results clearly indicated that plant allelochemicals did not change spinosad efficiency on H. armigera, but the combination of spinosad and plant allelochemicals might affect pest population dynamics significantly by changing its development.
文摘The present study achieves the biochemistry partial characterization of the chitinolytic extract produced by an endophytic Streptomyces sp. strain (A8). This extract was also tested against Anthonomus grandis, the cotton boll weevil aiming its control. The chitinase crude extract from the A8 strain was cultured for five days in a minimum liquid media supplemented with chitin. The extract was partially characterized by standard methods. The chitinolytic extract had an optimum temperature of 66 "C and an optimum pH between 4 to 9 (around 80% of relative activity). We also characterized the temperature and pH stability and measured the effects of enzyme inhibitors. The filtered chitinolytic extract was added to an artificial boll weevil diet. Boll weevil development from the egg stage to the adult stage was prolonged, and the percentage of adults that emerged was approximately 66% less than on control diet. This study showed that the.larval development of A. grandis was inhibited by the presence of characterized chitinolytic extract in artificial diet. This work provides an experimental basis for using the chitinase from an endophytic bacterium Streptomyces sp. as a biocontrol alternativeto controlling the plant pest A. grandis.