Poly(ε-caprolactone)(PCL)holds unique bioresorbability and competent biomechanical properties for tissueengineering application.However,PCL is hydrophobic intrinsically and poor in cell-biomaterial interaction.In thi...Poly(ε-caprolactone)(PCL)holds unique bioresorbability and competent biomechanical properties for tissueengineering application.However,PCL is hydrophobic intrinsically and poor in cell-biomaterial interaction.In this study,we prepared a composite based on PCL and bioactive tantalum(Ta)to understand the effects of direct laser micropatterning on composite surface properties.The PCL/Ta composite after preparation was surface-patterned by femtosecond laser and characterized with surface morphology,crystal structure,chemical composition,wettability and cellular response of fibroblast.It was found that laser micropatterning enlarged the difference of wetting properties(~15°)on PCL and PCL/Ta surfaces.The wetting changes was dependent on both material composition and lasermachined geometry.The blending of Ta enhanced surface wettability with prolonged contact time on the laser-machined line and rectangle microarrays.In vitro culture results showed beneficial effects of laser micropatterning on cell morphology of the fibroblasts.On the PCL/Ta surfaces with line and rectangle microarrays,the cells were more likely to bridge the sidewalls of the microgrooves,showing adaptive 3D morphologies to the micro/nano topographies on the sidewalls.These findings are envisaged to facilitate surface design and micropattern optimization for favorable tuning the cell response to biomedical PCL/Ta composites.展开更多
Plant organs are derived from stem cells.Once a leaf primordium initiates growth from the shoot apical meristem（SAM）,it establishes adaxial-abaxial（dorsal-ventral）polarity.This polarity essentially allows the leaf...Plant organs are derived from stem cells.Once a leaf primordium initiates growth from the shoot apical meristem（SAM）,it establishes adaxial-abaxial（dorsal-ventral）polarity.This polarity essentially allows the leaf to become a flat structure with the lamina expanding along the juxtaposition,i.e.,the middle domain,between the adaxial and abaxial domains.展开更多
基金Project(LY19A040001)supported by the Natural Science Foundation of Zhejiang Province,ChinaProjects(12147219,12035006)supported by the National Natural Science Foundation of China+1 种基金Project(531107050927)supported by the Fundamental Research Funds for the Central Universities,ChinaProject(JY-Q/008/2016)supported by the Hunan University for the Yuelu Young Scholars,China。
文摘Poly(ε-caprolactone)(PCL)holds unique bioresorbability and competent biomechanical properties for tissueengineering application.However,PCL is hydrophobic intrinsically and poor in cell-biomaterial interaction.In this study,we prepared a composite based on PCL and bioactive tantalum(Ta)to understand the effects of direct laser micropatterning on composite surface properties.The PCL/Ta composite after preparation was surface-patterned by femtosecond laser and characterized with surface morphology,crystal structure,chemical composition,wettability and cellular response of fibroblast.It was found that laser micropatterning enlarged the difference of wetting properties(~15°)on PCL and PCL/Ta surfaces.The wetting changes was dependent on both material composition and lasermachined geometry.The blending of Ta enhanced surface wettability with prolonged contact time on the laser-machined line and rectangle microarrays.In vitro culture results showed beneficial effects of laser micropatterning on cell morphology of the fibroblasts.On the PCL/Ta surfaces with line and rectangle microarrays,the cells were more likely to bridge the sidewalls of the microgrooves,showing adaptive 3D morphologies to the micro/nano topographies on the sidewalls.These findings are envisaged to facilitate surface design and micropattern optimization for favorable tuning the cell response to biomedical PCL/Ta composites.
文摘Plant organs are derived from stem cells.Once a leaf primordium initiates growth from the shoot apical meristem（SAM）,it establishes adaxial-abaxial（dorsal-ventral）polarity.This polarity essentially allows the leaf to become a flat structure with the lamina expanding along the juxtaposition,i.e.,the middle domain,between the adaxial and abaxial domains.
