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不同生物型烟粉虱体内Wolbachia共生菌的检测及其系统树分析 被引量:14
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作者 褚栋 丛斌 +3 位作者 张友军 徐宝云 吴青君 朱国仁 《昆虫学报》 CAS CSCD 北大核心 2005年第4期518-525,共8页
Wolbachia是广泛分布于节肢动物体内一类共生细菌,它能够通过多种机制调控宿主的生殖方式。近年来的研究表明,Wolbachia与许多外来生物的成功入侵相关。本文利用长PCR的方法特异扩增了不同生物型烟粉虱(共24个种群)体内Wolbachia的wsp基... Wolbachia是广泛分布于节肢动物体内一类共生细菌,它能够通过多种机制调控宿主的生殖方式。近年来的研究表明,Wolbachia与许多外来生物的成功入侵相关。本文利用长PCR的方法特异扩增了不同生物型烟粉虱(共24个种群)体内Wolbachia的wsp基因,结果发现B型和Q型烟粉虱入侵种群体内均未检测到Wolbachia,而在非B/Q型的浙江种群和肯尼亚种群体内检测到了Wolbachia。对该wsp基因进行测序并和已知序列进行同源性分析发现,浙江烟粉虱种群的Wolbachia属于B组Con/Rug亚种群,而肯尼亚种群属于B组Btab1亚种群。Wolbachia的存在与否可能与烟粉虱的成功入侵有一定的关系。 展开更多
关键词 生物型烟粉虱 WOLBACHIA 共生菌 检测 系统树 生物入侵 支序分析 害虫
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USING RAPD\|PCR TO DISTINGUISH BIOTYPES OF BEMISIA TABACI (HOMOPTERA:ALEYRODIDAE) IN CHINA 被引量:10
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作者 吴杏霞 胡敦孝 +1 位作者 李正西 沈佐锐 《Entomologia Sinica》 CSCD 2002年第3期1-8,共8页
Ten populations of the whitefly Bemisia tabaci (Gennadius) collected from eight fieed and ornamental crops from seven provinces/cities in China were determined using RAPD PCR. Results showed that the 10 populati... Ten populations of the whitefly Bemisia tabaci (Gennadius) collected from eight fieed and ornamental crops from seven provinces/cities in China were determined using RAPD PCR. Results showed that the 10 populations of B.tabaci could be classified into three distinct groups: a Biotype B group, a Guangxi group, and a Fujian group. The whiteflies in the Biotype B group were collected from sweetpotato and cotton in Beijing, tomato in Shandong, poinsettia in Shanghai and Guangdong, cabbage in Fujian, and hemp and eggplant in Xinjiang. The Guangxi group were collected from pumpkin in Nanning, Guangxi, and the Fujian group, from sweetpotato in Fujian. It was notable that B.tabaci specimens collected on cabbage in Fujian belonged to the Biotype B group. It is clear that Biotype B does now occur in China, in Beijing, Shandong, Xinjiang, Shanghai, Fujian and Guangdong. It is likely that the Guangxi and Fujian groups are native to China. 展开更多
关键词 Bemisia tabaci B Biotype RAPD PCR
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