[Objective] This study was conducted to establish a microbial limit test method for Bupi Qiangli Ointment. [Method] The conventional method and medium dilution method were used for bacterial, mold and yeast counting i...[Objective] This study was conducted to establish a microbial limit test method for Bupi Qiangli Ointment. [Method] The conventional method and medium dilution method were used for bacterial, mold and yeast counting in sample recovery test. [Result] The medium dilution method （1：10 test solution, 0.5 ml/plate） could effectively eliminate the inhibition effect of the Bupi Qiangli Ointment, and the recovery of Staphylococcus aureus was greater than 70% in the 3 batches of samples; and the conventional method exhibited the recoveries of E. coil, Bacillus subtilis, Candida albicans and Aspergillus greater than 70% in the 3 batches of samples. [Conclusion] Due to Bupi Qiangli Ointment has strongly antibacterial effect on Staphylococcus au- reus, the medium dilution method was used for bacterial counting, and the conventional method was used for mold and yeast counting; and the conventional method was used for controlled bacterium examination of E. coll.展开更多
Eutrophication, which is the enrichment of a water mass with inorganic and organic nutrients that support plant growth, is a key factor in stimulating phytoplankton growth. In this study, we determined the effects of ...Eutrophication, which is the enrichment of a water mass with inorganic and organic nutrients that support plant growth, is a key factor in stimulating phytoplankton growth. In this study, we determined the effects of various nitrogen sources, different nitrogen concentrations in the culture medium, and two culture methods on the growth of the green alga, Enteromorpha prolifera. The relationship between the specific growth rate of E. prolifera and NO3-N concentration was consistent with that estimated using the Monod equation （R2=0.9713, P〈0.01）. In the N O3-N medium, the maximum specific growth rate was calculated to be 0.1634/d and the semi-saturation constant was calculated to be 16.86 μmol/L. Our results show that E. prolijkra can effectively utilize NH4^＋-N, NO3^-N, and NO^2-N and urea-N in the range of 5 to 50 μmol/L. NH4^＋-N was preferentially assimilated by E. prolifera, and urea-N was favorable for long-term growth.展开更多
Our field experiments showed that the cultivation of pearl mussels formed a new biocoene composed of filamentous algae, protozoa, porifera and coelenterate. It effectively reduced nitrogen, phosphorus, chemical oxygen...Our field experiments showed that the cultivation of pearl mussels formed a new biocoene composed of filamentous algae, protozoa, porifera and coelenterate. It effectively reduced nitrogen, phosphorus, chemical oxygen demand and biochemical oxygen demand in the water by 67.3%, 73.2%, 38.1% and 15.5%, respectively, during May to September 1998 when the water eutrophication was developing. This could control water eutrophication and produce pearls, shellfish meat and shells. This is an economical and effective way to control water eutrophication by using the ultra strong filtering capability of freshwater pearl mussels.展开更多
基金Supported by Traditional Chinese Medicine and Ethnic Minority Medicine Technology Research Subject of Guizhou Administrative Bureau(QZYY2013-70,QZYY-2014-026)Joint Application of Basic Research Fund of Science and Technology Department o Yunnan Province-Guiyang University of Chinese Medicine(QKHLHZ[2014]7323)~~
文摘[Objective] This study was conducted to establish a microbial limit test method for Bupi Qiangli Ointment. [Method] The conventional method and medium dilution method were used for bacterial, mold and yeast counting in sample recovery test. [Result] The medium dilution method （1：10 test solution, 0.5 ml/plate） could effectively eliminate the inhibition effect of the Bupi Qiangli Ointment, and the recovery of Staphylococcus aureus was greater than 70% in the 3 batches of samples; and the conventional method exhibited the recoveries of E. coil, Bacillus subtilis, Candida albicans and Aspergillus greater than 70% in the 3 batches of samples. [Conclusion] Due to Bupi Qiangli Ointment has strongly antibacterial effect on Staphylococcus au- reus, the medium dilution method was used for bacterial counting, and the conventional method was used for mold and yeast counting; and the conventional method was used for controlled bacterium examination of E. coll.
基金Supported by the Major Projects of Knowledge Innovation Program of Chinese Academy of Sciences (No. KZCX2-SW-208-01)the National Natural Science Foundation of China (No. 40976047)National Basic Research Program of China (973 Program) (No. 2010CB428701)
文摘Eutrophication, which is the enrichment of a water mass with inorganic and organic nutrients that support plant growth, is a key factor in stimulating phytoplankton growth. In this study, we determined the effects of various nitrogen sources, different nitrogen concentrations in the culture medium, and two culture methods on the growth of the green alga, Enteromorpha prolifera. The relationship between the specific growth rate of E. prolifera and NO3-N concentration was consistent with that estimated using the Monod equation （R2=0.9713, P〈0.01）. In the N O3-N medium, the maximum specific growth rate was calculated to be 0.1634/d and the semi-saturation constant was calculated to be 16.86 μmol/L. Our results show that E. prolijkra can effectively utilize NH4^＋-N, NO3^-N, and NO^2-N and urea-N in the range of 5 to 50 μmol/L. NH4^＋-N was preferentially assimilated by E. prolifera, and urea-N was favorable for long-term growth.
文摘Our field experiments showed that the cultivation of pearl mussels formed a new biocoene composed of filamentous algae, protozoa, porifera and coelenterate. It effectively reduced nitrogen, phosphorus, chemical oxygen demand and biochemical oxygen demand in the water by 67.3%, 73.2%, 38.1% and 15.5%, respectively, during May to September 1998 when the water eutrophication was developing. This could control water eutrophication and produce pearls, shellfish meat and shells. This is an economical and effective way to control water eutrophication by using the ultra strong filtering capability of freshwater pearl mussels.
