[Objective] The aim of this study was to identify the roles of an aquaporin gene GhNIP5.1 in upland cotton （Gossypium hirsutum） by bioinformatics method, so as to provide theoretical basis for further research on aq...[Objective] The aim of this study was to identify the roles of an aquaporin gene GhNIP5.1 in upland cotton （Gossypium hirsutum） by bioinformatics method, so as to provide theoretical basis for further research on aquaporins in upland cotton. [Method] In silico molecular cloning was adopted to obtain an ORF sequence of GhNIP5.1 gene, which was then analyzed by the methods of bioinformatics. The coding region of GhNIP5.1 gene was obtained by analyzing the cotton genome se-quence published in NCBI. [Result] This cDNA sequence had a complete open reading frame of 897 bp and encoded 298 amino acid residues, including the con-served domain NPA （Asn-Pro-Ala） of MIP superfamily. The similarities of GhNIP5.1 deduced amino acid sequences from upland cotton with grape and Arabidopsis, were up to 89.3% and 83.2%, respectively. GhNIP5.1 was most similar in homology and 3-D structure of proteins to AtNIP5.1 among the nine members of NIP family in Arabidopsis. The coding region length of GhNIP5.1 gene was 2 067 bp, and it con-tained three introns and four exons. Al the exon-intron junctions of the gene con-tained the consensus splicing site pair GT-AG. [Conclusion] GhNIP5.1 gene probably has similar physiological functions with Arabidopsis AtNIP5.1.展开更多
Objective: To investigate the effect of substance P (SP) on gene expression of transforming growth factor β-1 (TGFβ-1), transforming growth factor receptor-1 (TGFR-1) and transforming growth factor receptor-2 (TGFR-...Objective: To investigate the effect of substance P (SP) on gene expression of transforming growth factor β-1 (TGFβ-1), transforming growth factor receptor-1 (TGFR-1) and transforming growth factor receptor-2 (TGFR-2) in fibroblasts cultured in vitro from rat’s granulation tissues. Methods: The fibroblasts from the granulation tissues in the skeletal muscle of rat’s hind limbs injured by formaldehyde were cultured in vitro. When different concentrations (10 -9-10 -5 mol/L) of SP were added into the culture medium, the changes of gene expression of TGFβ-1, TGFR-1 and TGFR-2 in the cultured fibroblasts were observed with reverse transcription polymerase chain reaction at different intervals (0, 3, 6, 12 and 24 hours after incubation). Results: The gene expression of TGFβ-1, TGFR-1 and TGFR-2 in the fibroblasts cultured from rat’s granulation tissues was up-regulated by SP. The peak level of the mRNA expression was found at 10 -8 mol/L SP and the up-regulation effect was not found at 10 -5 mol/L and 10 -6 mol/L. The peak levels of gene expression of TGFβ-1, TGFR-1 and TGFR-2 in the fibroblasts treated with SP were achieved at 6 and 12 hours, respectively. Conclusions: SP has up-regulation effect on the gene expression of TGFβ-1, TGFR-1 and TGFR-2 in fibroblasts from rat’s granulation tissues in vitro, and the effect is related to different stimulating concentrations of SP. It may be concerned with proliferation and differentiation of fibroblasts and formation of scar tissues during wound healing.展开更多
基金Supported by National Natural Science Foundation of China(31301682)AgriculturalScience and Technology Innovation Program of Jiangsu Province[CX(12)3068]SpecialProject for Breeding and Cultivation of GMO Varieties(2011ZX08005-001)~~
文摘[Objective] The aim of this study was to identify the roles of an aquaporin gene GhNIP5.1 in upland cotton （Gossypium hirsutum） by bioinformatics method, so as to provide theoretical basis for further research on aquaporins in upland cotton. [Method] In silico molecular cloning was adopted to obtain an ORF sequence of GhNIP5.1 gene, which was then analyzed by the methods of bioinformatics. The coding region of GhNIP5.1 gene was obtained by analyzing the cotton genome se-quence published in NCBI. [Result] This cDNA sequence had a complete open reading frame of 897 bp and encoded 298 amino acid residues, including the con-served domain NPA （Asn-Pro-Ala） of MIP superfamily. The similarities of GhNIP5.1 deduced amino acid sequences from upland cotton with grape and Arabidopsis, were up to 89.3% and 83.2%, respectively. GhNIP5.1 was most similar in homology and 3-D structure of proteins to AtNIP5.1 among the nine members of NIP family in Arabidopsis. The coding region length of GhNIP5.1 gene was 2 067 bp, and it con-tained three introns and four exons. Al the exon-intron junctions of the gene con-tained the consensus splicing site pair GT-AG. [Conclusion] GhNIP5.1 gene probably has similar physiological functions with Arabidopsis AtNIP5.1.
基金ThisworkwassupportedbyMajorStateBasicResearchDevelopmentProgramofChina (No .G19990 5 4 2 0 4 )
文摘Objective: To investigate the effect of substance P (SP) on gene expression of transforming growth factor β-1 (TGFβ-1), transforming growth factor receptor-1 (TGFR-1) and transforming growth factor receptor-2 (TGFR-2) in fibroblasts cultured in vitro from rat’s granulation tissues. Methods: The fibroblasts from the granulation tissues in the skeletal muscle of rat’s hind limbs injured by formaldehyde were cultured in vitro. When different concentrations (10 -9-10 -5 mol/L) of SP were added into the culture medium, the changes of gene expression of TGFβ-1, TGFR-1 and TGFR-2 in the cultured fibroblasts were observed with reverse transcription polymerase chain reaction at different intervals (0, 3, 6, 12 and 24 hours after incubation). Results: The gene expression of TGFβ-1, TGFR-1 and TGFR-2 in the fibroblasts cultured from rat’s granulation tissues was up-regulated by SP. The peak level of the mRNA expression was found at 10 -8 mol/L SP and the up-regulation effect was not found at 10 -5 mol/L and 10 -6 mol/L. The peak levels of gene expression of TGFβ-1, TGFR-1 and TGFR-2 in the fibroblasts treated with SP were achieved at 6 and 12 hours, respectively. Conclusions: SP has up-regulation effect on the gene expression of TGFβ-1, TGFR-1 and TGFR-2 in fibroblasts from rat’s granulation tissues in vitro, and the effect is related to different stimulating concentrations of SP. It may be concerned with proliferation and differentiation of fibroblasts and formation of scar tissues during wound healing.
