降低灯泡贯流式机组主轴密封水管清洗频次的措施研究

运用PDCA循环来降低灯泡贯流式机组主轴密封水管清洗频次,并针对要因制定相应的对策及措施,以供参考。

Expression of 6 MicroRNAs in Prostate Cancer and Its Significance

OBJECTIVE Numerous microRNAs (miRNAs) are deregulatedin human cancers. The experimental evidence supports thatmiRNAs plays a role in the initiation and progression of humanmalignancies.The present study was undertaken to evaluatethe differential expression of 6 miRNAs as biomarker for earlydetection of prostate cancer, and then to determine whether theexpression profiling of these miRNAs could predict the prognosisof prostate cancer.METHODS The expression profilings of these 6 miRNAs wereinvestigated using the method of locked nucleic acid (LNA)-modified oligonucleotide in situ hybridization (ISH). And thetechnology of tissue microarray (TMA) was employed using theformalin-fixed, paraffin-embedd (FFPE) specimens taken from52 patients with prostate carcinoma (PCa) and 38 patients withbenign prostatic hyperplasia (BPH).RESULTS The rates of positive expression for 6 miRNAs (miR-15b, miR-16, let-7g, miR- 96,miR-182 and miR-183) were 26.92%,15.38%, 15.38%, 67.31%, 61.54% and 71.15% in the specimens ofprostate cancer, and 57.89%, 76.32%, 68.42%, 44.74%, 31.58%,47.37% in the tissues of benign prostatic hyperplasia, respectively.The expressions of all 6 miRNAs between the prostate cancer andbenign prostatic hyperplasia tissues were significantly different(P < 0.05). The positive rate of these 6 miRNAs was significantlyrelated to the Gleason Grading of prostate cancer (P < 0.01). Therewas no significant correlation between the expression of thesemiRNAs and age and the concentration of serum PSA of thepatient (P >0.05). We also found that the expression of miR-15b,miR-96 and miR-182 correlated with clinical stages of tumor (P <0.05). The expression of miR-96 correlated with lobus prostatae oftumor invasion (P < 0.01), but the expressions of the remaining fivemiRNAs were not correlated with that (P >0.05). In addition, theexpression of miR-15b was negatively related to that of miR-96,miR-182 and miR-183, respectively (P < 0.01, r < 0.00).There wasa positive correlation among the expressions of miR-96, miR-182and miR-183 in prostate cancer (P < 0.01, r >0.00). The expressionof miR-16 was positively related to that of miR-let-7g (P < 0.01, r >0.00).CONCLUSION The results suggest that miRNA expressionprofiling could have relevance to the biological and clinicalbehavior of prostate cancer,and they might be importantbiomarkers for early detection and prognostic assessment ofprostate cancer.

Expression of DLK1 Gene in the Bone Marrow Cells of Patients with Myelodysplastic Syndromes and Its Clinical Significance

Objective This study aims to investigate the expression of delta-like 1 （DLK1） gene in the bone marrow cells of patients with myelodysplastic syndromes （MDS） and to explore its molecular characteristics for the early diagnosis of MDS. Methods The expression of DLK1 mRNA in the bone marrow cells of cases with MDS, acute myeloid leukemia （AML）, and normal control groups were measured by real-time polymerase chain reaction and were analyzed for clinical significance. Results Significantly higher expression of DLK1 mRNA was observed in the bone marrow cells of MDS patients （0.7342±0.3652） compared with the normal control group （0.4801±0.1759） （P〈0.05）. The expression of DLK1 mRNA had a positive correlation with the proportion of bone marrow blasts （r=0.467, P〈0.05）. Moreover, DLK1 mRNA expression was significantly increased as MDS progressed （P〈0.05）. Patients with abnormal karyotypes exhibited significantly higher expression of DLK1 mRNA （0.9007±0.4334） than those with normal karyotypes （0.6411±0.2630） （P〈0.05）. Subsequently, patients with highly expressed DLK1 （≥0.8） presented significantly higher malignant clone burden （0.4134±0.3999） than those with lower DLK1 expression （〈0.8）,（0.1517±0.3109）, （P〈0.05）. Conclusions The DLK1 gene was highly expressed in MDS patients, and was increased as MDS progressed. The expression of DLK1 mRNA was positively correlated with the proportion of the bone marrow blasts. A high expression of DLK1 gene suggested a higher malignant clone burden of MDS.

Ecological functions of ciliated protozoa in marine ecosystem： effects of ammonium on the population growth of Euplotes vannus

The effects of ammonium on the population growth of the marine ciliate, Euplotes vannus, were examined using ecotoxicological method, h is showed that ammonium exerts inhibitory effects on the growth of the ciliate populations in a concentration-dependent way. Statistical analvsis reveals that the population growth dynamics exposed to ammoniunt-N concentration over 100mg/L are significantly different from that in the control at P 〈 0.05 level. Linear regression determined that the 24h, 36h, 48h, 60h, 72h and 84h IC50 values of ammonium-N are 19.68, 201.51, 167.49, 47.86, 50.43 and 43.11 concentration over 100mg/L, respectively （ P 〈 0.05; pH 8.2; salinity 28 ppt; temperature, 25℃ ）. The results indicate that the tolerance to ammonium in E. vannus is considerably higher than that of the larvae or juveniles of some metozoa, such as cuhured prawns and oysters. Therefore, it is believed that the high tolerance to ammonium is necessary for eiliated protozoa to play positive roles in maintaining and improving water quality in marine ecosystems, especially in the intensive aquacuhure waters with high-level ammonium. In addition, the correlation between /C50 values and exposure time was discussed.

What Life Is and How It Originated

This paper refutes the mechanistic interpretation of cellular dynamics and contends that the life-giving principle is sustained growth a biological system and is uninterrupted growth balanced in a dynamic state by synthesis and dissolution. The process began by an oxidation/reduction reaction on the surface of pyrite energized photovoltaically by sunlight. Hydrogen sulfide was oxidized, carbon dioxide was reduced, and phosphate on the surface of the pyrite was a reactant. The first organic compounds were sulfides and phosphoglycerates. These organophosphates were at the center of the energy cycle of all life where the dehydration of a relatively unreactive ＂low-energy＂ two-phosphoglycerate transforms it into the ＂high-energy＂ phosphoenolpyruvate. Life began as a growth process and continues to grow ceaselessly out of necessity. It cannot discontinue the life-giving energy flow without irreparable loss of the process. All forms of life past and present were and are stabilized systems in which the growth process is contained in metabolic turnover.

Effects of Different NaCl Doses on Morphological and Physiological Parameters in Mentha piperita L.

Salt stress is one of the most serious limiting factors for crop growth and production. This study was carried out to evaluate the effects of salt stress in four Mentha piperita L. varieties, in greenhouse conditions. The seedlings were irrigated with 1/2 Hoagland nutrition solutions. Following 10 d of growth in the pots, the seedlings were subjected to salt stress with different concentrations of NaCl （0, 50 and 100 mM, respectively）. Responses of the mint varieties to salt stress were evaluated using some plant growth and physiological parameters, such as plant height, root length, shoot and root fresh and dry weight, and ion concentrations in leaves, such as Na, K, Ca and Cl. Salt stress caused reduction in physiological parameters. However, this reduction was significant at 100 mM NaCl compared to 50 mM NaCl. These two concentrations of NaCl increased Na and Cl contents in leaves. However, K and Ca contents were decreased with salinity. The results showed that Mentha piperita cv. Sewiss and Granada were found more tolerant to salt stress than cv. Marokko and Citaro.

Population growth, trophic level, and reproductive biology of two congeneric archer fishes (Toxotes chatareus, Hamilton 1822 and Toxotes jaculatrix, Pallas 1767) inhabiting Malaysian coastal waters

Population growth, trophic level, and some aspects of reproductive biology of two congeneric archer fish species, Toxotes chatareus and Toxotes jaculatrix, collected from Johor coastal waters, Malaysia, were studied. Growth pattern by length-weight relationship (W=aLb) for the sexes differed, and exhibited positive allometric growth (male, female and combined sexes of T. chatareus; female and combined sexes of T. jaculatrix) and isometric growth (male samples of T. jaculatrix only). Trophic levels of both species were analyzed based on 128 specimens. The results show that, in both species, crustaceans and insects were the most abundant prey items, and among crustaceans the red clawed crab Sesarma bidens and Formicidae family insects were the most represented taxa. The estimated mean trophic levels for T. chatareus and T. jaculatrix were 3.422±0.009 and 3.420±0.020, respectively, indicating that they are largely carnivores. Fecundity of T. chatareus ranged from 38 354 to 147 185 eggs for females with total length ranging from 14.5 to 22.5 cm and total body weight from 48.7 to 270.2 g, and T. jaculatrix 25 251 to 150 456 eggs for females with total length ranging from 12.2 to 23.0 cm and total body weight from 25.7 to 275.0 g. Differences in values of gonadosomatic and hepatosomatic indexes calculated for both species in this study may have resulted from uneven sample size ranges.

Effects of latitudinal variation on field and common garden comparisons between native and introduced groundsel(Senecio vulgaris)populations

Aims Field and common garden comparisons are commonly performed to test the rapid evolution of increased vigor in introduced plant populations.Latitudinal dines in phenotypic traits can obscure such evolutionary inferences,particularly when native or introduced populations are distributed across large geographic ranges.We tested whether the latitudinal dines influence comparisons between introduced and native populations of Senecio vulgaris.Methods Senecio vulgaris is native to Europe but has been introduced in northeastern and southwestern China.To evaluate the performance in terms of growth and reproduction between native European populations and introduced Chinese populations,we compared plant height,number of branches and number of capitula in field populations in native and introduced ranges and in a common garden in Switzerland.Important Findings The introduced Chinese populations performed better than the native European populations in the field in terms of plant height and number of capitula,which was consistent with the prediction of the evolution of the increased competitive ability(EICA)hypothesis.The Chinese populations produced more capitula than the European populations when the latitudinal cline was considered in the common garden comparison.When we compared the traits of the northeastern Chinese,southwestern Chinese and European populations in both the field and common garden,the northeastern Chinese populations,at latitudes similar to those of the European populations,exhibited greater plant size and more capitula than the European populations in both the field and common garden.However,the southwestern Chinese populations,at latitudes much lower than those of the European populations,did not perform better than the native populations in terms of reproduction.In conclusion,our results suggest that latitudinal dines in phenotypic traits should be considered in field and common garden comparisons when introduced populations are geographically structured.