The comparative analysis of the biological characterization and the genetic background study of EV71 circulating strains is commonly recognized as basic work necessary for development of an effective EV71 vaccine. In ...The comparative analysis of the biological characterization and the genetic background study of EV71 circulating strains is commonly recognized as basic work necessary for development of an effective EV71 vaccine. In this study, we sequenced five EV71 circulating strains, isolated from Fuyang, Hefei, Kunming and Shenzhen city of China and named them FY-23, FY-22, H44, K9 and S1 respectively. The sequence alignment demonstrated their genotypes be C4. The genetic distance of the VP1 gene from these isolates suggested that they were highly co-related with genetic identity similar to other previously reported EV71 strains in China. Additionally, these strains were identified to display some obvious proliferation dynamics and plaque morphology when propagated in Vero cells. However, a distinctive difference in pathogenic ability in neonatal mice was found. Some differences in cross neutralization test & immunogenic analysis were also found. All these results are related to the biological characterization of circulating EV71 strains in China and aid in the development of an EV71 vaccine in the future.展开更多
第12届St．Gallen国际乳腺癌会议于2011-03—19在瑞士闭幕，共有来自96个国家和地区的4300名学者与会。早期乳腺癌的2011年St．Gallen国际专家治疗共识已于2011—06—27发布于欧洲肿瘤内科学会会刊“knnal of Oncology”。St．gallen国...第12届St．Gallen国际乳腺癌会议于2011-03—19在瑞士闭幕，共有来自96个国家和地区的4300名学者与会。早期乳腺癌的2011年St．Gallen国际专家治疗共识已于2011—06—27发布于欧洲肿瘤内科学会会刊“knnal of Oncology”。St．gallen国际乳腺癌治疗专家共识作为指导临床实践的重要参考，每两年更新一次。2007年该共识提出根据病人年龄、肿瘤大小、组织学分级、展开更多
基金National Science and Technology Major Project (2008ZX10004-014)
文摘The comparative analysis of the biological characterization and the genetic background study of EV71 circulating strains is commonly recognized as basic work necessary for development of an effective EV71 vaccine. In this study, we sequenced five EV71 circulating strains, isolated from Fuyang, Hefei, Kunming and Shenzhen city of China and named them FY-23, FY-22, H44, K9 and S1 respectively. The sequence alignment demonstrated their genotypes be C4. The genetic distance of the VP1 gene from these isolates suggested that they were highly co-related with genetic identity similar to other previously reported EV71 strains in China. Additionally, these strains were identified to display some obvious proliferation dynamics and plaque morphology when propagated in Vero cells. However, a distinctive difference in pathogenic ability in neonatal mice was found. Some differences in cross neutralization test & immunogenic analysis were also found. All these results are related to the biological characterization of circulating EV71 strains in China and aid in the development of an EV71 vaccine in the future.
文摘第12届St．Gallen国际乳腺癌会议于2011-03—19在瑞士闭幕，共有来自96个国家和地区的4300名学者与会。早期乳腺癌的2011年St．Gallen国际专家治疗共识已于2011—06—27发布于欧洲肿瘤内科学会会刊“knnal of Oncology”。St．gallen国际乳腺癌治疗专家共识作为指导临床实践的重要参考，每两年更新一次。2007年该共识提出根据病人年龄、肿瘤大小、组织学分级、
