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鼠伤寒沙门菌三型分泌系统效应蛋白SseK3生物学底物的鉴定及其功能的初步研究
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作者 孟昆 石柳柳 +5 位作者 潘兴 杨瑾 薛娟 朱平 吕均 李姗 《中华微生物学和免疫学杂志》 CAS CSCD 北大核心 2021年第11期860-866,共7页
目的鉴定SseK3蛋白宿主中新的生物学底物以及对其功能的影响。方法通过酵母双杂交系统(yeast-two-hybrid,Y2H)从HeLa cDNA文库中筛选SseK3潜在结合蛋白;通过细胞共表达和体外活性试验检测SseK3对底物蛋白的精氨酸N-乙酰葡萄糖胺化(Arg-G... 目的鉴定SseK3蛋白宿主中新的生物学底物以及对其功能的影响。方法通过酵母双杂交系统(yeast-two-hybrid,Y2H)从HeLa cDNA文库中筛选SseK3潜在结合蛋白;通过细胞共表达和体外活性试验检测SseK3对底物蛋白的精氨酸N-乙酰葡萄糖胺化(Arg-GlcNAc)修饰;通过点突变检测SseK3对底物蛋白的修饰位点;通过免疫共沉淀试验检测底物蛋白的Arg-GlcNAc修饰对其互作蛋白结合能力的影响。结果酵母双杂交和基因测序结果表明,SseK3的一个全新底物是Snapin;SseK3在细胞内和体外均可以Arg-GlcNAc修饰Snapin;修饰位点位于Snapin蛋白C端的119和120位精氨酸;Arg-GlcNAc修饰Snapin抑制了Snapin-SNAP25的结合。结论本研究成功筛选到SseK3的一个新的宿主底物蛋白Snapin,初步研究了SseK3对Snapin的Arg-GlcNAc修饰及该修饰对Snapin功能的影响,为后续进一步理解细菌的Arg-GlcNAc修饰功能以及在病原微生物感染过程中的作用机制提供理论依据。 展开更多
关键词 沙门菌 三型分泌系统效应蛋白 SseK3 生物学底物
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Inocula selection in microbial fuel cells based on anodic biofilm abundance of Geobacter sulfurreducens 被引量:1
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作者 Guotao Sun Diogo de Sacadura Rodrigues +3 位作者 Anders Thygesen Geoffrey Daniel Dinesh Fernando Anne S.Meyer 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2016年第3期379-387,共9页
Microbial fuel cells (MFCs) rely on microbial conversion of organic substrates to electricity. The optimal perfor- mance depends on the establishment of a microbial community rich in electrogenic bacteria. Usually t... Microbial fuel cells (MFCs) rely on microbial conversion of organic substrates to electricity. The optimal perfor- mance depends on the establishment of a microbial community rich in electrogenic bacteria. Usually this micro- bial community is established from inoculation of the MFC anode chamber with naturally occurring mixed inocula. In this study, the electrochemical performance of MFCs and microbial community evolution were eval- uated for three inocula including domestic wastewater (DW), lake sediment (LS) and biogas sludge (BS) with varying substrate loading (Lsub) and external resistance (Rext) on the MFC. The electrogenic bacterium Geobacter sulfurreducens was identified in all inocula and its abundance during MFC operation was positively linked to the MFC performance. The IS inoculated MFCs showed highest abundance (18% ± 1%) of G. sulfurreducens, maximum current density [Imax = (690 ± 30) mA.m 2] and coulombic efficiency (CE = 29% ±1%) with acetate as the substrate./max and CE increased to (1780 ± 30) mA.m-2 and 58%± 1%, respectively, after decreasing the Rext from 1000 Ωto 200 Ω, which also correlated to a higher abundance ofG. sulfurreducens (21% ±0.7%) on the MFC anodic biofilm. The data obtained contribute to understanding the microbial community response to Lsub and Roy, for of timizing electricity eneration in MFCs. 展开更多
关键词 Lake sediment Coulombic efficiency Denaturing gradient gel electrophoresis Geobacter sulfurreducens Anode polarisation resistance
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Biomechanical study of human dura and its substitutes 被引量:1
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作者 周良辅 宋冬雷 丁祖荣 《Chinese Medical Journal》 SCIE CAS CSCD 2002年第11期1657-1659,150,共3页
OBJECTIVE: To investigate the biomechanical characters of human dura and its substitutes and to establish guidelines for selection of optimal repair materials for reconstruction of skull base defects. METHODS: A measu... OBJECTIVE: To investigate the biomechanical characters of human dura and its substitutes and to establish guidelines for selection of optimal repair materials for reconstruction of skull base defects. METHODS: A measurement of creep properties of human dura and its substitutes were conducted using biomechanical tests. The dynamic changes of biomechanical properties of canine dura obtained from skull base defects were observed as well. RESULTS: The creep properties of human dura presented a linear-relationship between initial strain and stress, and the creep strain increased slowly with time. The creep compliance formula for human dura and its substitutes was as follows: J (t) = J(0) + Kf (t). The initial compliance of canine dura in skull base defects was reduced by 35%, 46% and 50%, respectively 1, 3 and 6 months after surgery. CONCLUSION: The optimal material for the repair of skull base defects can be estimated using creep compliance analysis. The less the compliance, the better the property of anti-protrusion. Fresh human dura is the least compliant and then in increasing order are lyophilized human dura, fresh human pericranium, Terylene and silicon membranes. The pattern of biomechanical characteristics of the dura mater at skull base defects shows that the strain ability of the dura mater decreases distinctly for 1 - 3 months and then remains almost unchanged for 3 - 6 months after surgery. 展开更多
关键词 ANIMALS BIOMECHANICS DOGS Dura Mater Humans Research Support Non-U.S. Gov't Skull Base
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Effects of Biochar and Super Absorbent Polymer on Substrate Properties and Water Spinach Growth 被引量:16
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作者 FAN Ruqin LUO Jia +2 位作者 YAN Shaohua ZHOU Yunlai ZHANG Zhenhua 《Pedosphere》 SCIE CAS CSCD 2015年第5期737-748,共12页
Most previous researches have focused on biochar application in agricultural soils; however, limited information is available concerning the effects of biochar amendment on greenhouse substrate properties. A greenhous... Most previous researches have focused on biochar application in agricultural soils; however, limited information is available concerning the effects of biochar amendment on greenhouse substrate properties. A greenhouse experiment was conducted to investigate effects of wheat straw biochar (0-160 mL L-1) and super absorbent polymer (SAP, 0.8 g L-1) on physical and chemical properties of a substrate based on spent pig litter compost and the growth of water spinach (Ipomoea aquatica Forsk). Total porosity, water-holding capacity~ pH and electrical conductivity (EC) of the substrate significantly increased with increasing biochar rates, especially in the substrate without SAP. The values of pH and EC were significantly lower in the substrate with SAP than those without SAP at the high biochar application rates (100-160 mL L-l). The germination rates of water spinach decreased with increasing biochar rates when biochar was added alone (76.9%-83.7%), whereas the rates increased to 83.6%-85.8% when biochar was added in combination with SAP. Growth parameters of water spinach and nutrient uptake by shoots and roots increased with increasing biochar rates and reached the maximum values at the biochar rate of 100 mL L-1. There were significant cubic relationships between the uptake of nutrients (N, P, and K) and biochar rates, both with and without SAP addition. In order to avoid negative effects on plant growth, the biochar application rate should be controlled at an optimal level (100 mL L-1 ). The SAP addition not only enhanced the positive effects of biochar application on the properties of the substrate, but also inhibited the excessive rise of pH and EC following biochar additions, which led to better plant growth and enhanced nutrient uptakes by water spinach. 展开更多
关键词 AMENDMENT electrical conductivity nutrient uptake PH total porosity water-holding capacity water spinach wheat straw
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