超声及声触诊组织量化技术在囊型肝包虫生物学活力判断中的应用价值

目的探讨超声及声触诊组织量化(VTQ)技术在囊型肝包虫囊肿内容物活性判断中的应用价值。方法通过超声检查和VTQ技术,检测48例经手术病理证实的51个囊型肝包虫囊肿声像图特征及囊内容物的Vs值,并结合病理学检查资料进行比较分析。结果囊型肝包虫超声分型中,内囊分离型和实变型分别与单纯囊肿型和子囊型比较,Vs测量阳性率均增高(P<0.05);无活性原头蚴组Vs测量阳性率比有活性原头蚴组显著增高(P<0.001);病理学分级中,Ⅲ级分别与I级及Ⅱ级比较,Vs测量阳性率均增高(P<0.05);内囊破裂组Vs测量阳性率显著高于内囊完整组(P<0.001)。结论 VTQ技术结合超声声像图,在囊型肝包虫内容物活性预测中具有较高的临床应用价值。

Fas Mcab对病理性瘢痕成纤维细胞生物学活力的影响

目的 探讨FasMcab对病理性瘢痕成纤维细胞生物学活力的影响。方法 以体外培养的病理瘢痕成纤维细胞为研究对象 ,应用MTT法 ,3H -TDR核苷掺入法 ,观察了FasMcab对增生性瘢痕成纤维细胞活力的影响 ;应用流式细胞仪检测FasMcab作用下 ,增生性瘢痕和瘢痕疙瘩两类成纤维细胞的凋亡率。结果 ①低浓度FasMcab对增生性瘢痕成纤维细胞的活力具有促进作用 ;②FasMcab在 4 μg/ml的浓度下可迅速诱导增生性瘢痕成纤维细胞的凋亡 ,但在相同条件下对瘢痕疙瘩成纤维细胞不具有诱导凋亡的作用。结论 瘢痕疙瘩成纤维细胞对FasMcab诱导的凋亡存在抗性。深入研究抗性产生的原因将有助于揭示病理性瘢痕增生的形成机理。

CT在囊型肝包虫活性判断中的应用价值

目的:探讨CT对囊型肝包虫活性特征影像学表现判断的价值。方法:40例经手术切除病理证实的共42个肝细粒棘球蚴包虫囊的影像学及术后记录囊内容物性状(液性,胶胨性,实变钙化)及内囊内原头节活性及内囊HE染色切片的镜下结构。结果:不同类型的包虫囊内其CT值差异有显著性意义(P<0.05);实变钙化组未见活性原头蚴的存在;CT显示囊内容物液态时囊内原头节活力明显高于胶冻性组(P<0.05),包虫母囊内原头蚴的活性随CT值的增大而减小;包虫内囊组织结构损害随内囊的剥离,塌陷而加重。结论:CT在包虫囊的活性程度的判断上具有重要的价值。

Effect of Si on growth kinetics of intermetallic compounds during reaction between solid iron and molten aluminum

The effect of Si on the growth kinetics of intermetallic compounds during the reaction of solid iron and molten aluminum was investigated with a scanning electron microscope coupled with an energy dispersive X-ray spectroscope, and hot-dip aluminized experiments. The results show that the intermetallic layer is composed of major Fe2Al5 and minor FeAl3. The Al-Fe-Si ternary phase, rl/rg, is formed in the Fe2Al5 layer. The tongue-like morphology of the Fe2Als layer becomes less distinct and disappears finally as the content of Si in aluminum bath increases. Si in the bath improves the prohibiting ability to the growth of Fe2Als and FeAl3. When the contents of Si are 0, 0.5%, 1.0%, 1.5%, 2.0% and 3.0%, the activation energies of Fe2Al5 are evaluated to be 207, 186, 169, 168, 167 and 172 kJ/mol, respectively. The reduction of the activation energy might result from the lattice distortion caused by Si atom penetrating into the Fe2Al5 phase. When Si atom occupies the vacancy site, it blocks easy diffusion path and results in the disappearance of tongue-like morphology.

Expression and Identification of Mycoplasma penetrans P35 Lipoprotein

Objective: To study the biological activity of Myco-plasma penetrans 35kDa lipoprotein(P35) in vitro, prokaryotic expression vector pQE31//p35 was constructed and recombinant fusion protein P35 (rP35) was expressed in E.coli. Methods: The p35 gene was amplified by polymerase chain reaction(PCR), cloned to pQE31, and a positive clone was screened. PCR-mediated mutagenesis was used to change the two "TGA" triplets to "TGG" triplets within the p35 gene. Production of the recombinant protein was induced by the addition of IPTG to the E.coli culture. rP35 was purified with a Ni-NTA Spin Kit and rP35 purification was analyzed by Western blot. Results: About 1Kb PCR amplification was cloned into pQE31. The two "TGA" triplets within the p35 gene were successfully changed to "TGG" triplets. The pQE31/p35 vector expressed a protein with a calculated molecular mass of 37.4kDa in E.coli. Western blot indicated the 37.4kDa protein was rP35 . Conclusion: PQE31/p35, a prokaryotic expression vector containing p35 gene, was successfully constructed and expressed in E.coli.

Influence of Prospecting and Crude Oil Production on Geoecological Parameters of Permafrost

The large reserves ofoil are disposed in zones of permafrost in shelf and continental fields. In Europe the subarctic and arctic tundra are abundant only in Nenetsky autonomous area and in the north-east of the republic of Komi. Oil and gas production activity has threatened richest biological resources and health of the population of the region. A singularity of petroliferous fields of the north of Russia is the existence of the thick strata of permafrost. On top of them different heat-insulating overlying layers （snow, biogenic covers） reside. State and dynamics of permafrost cause progressing dangerous exogenic geoecological processes, which are exhibited completely. State of permafrost is instituted by exchange of heat in near-surface strata of a geological section. During the development of oil fields there is a disturbance of a temperature schedule of permafrost. For estimation of technical attack the analytical computational method of influence of the different factors on value of annual heat exchange and prediction of geocryological conditions is proposed. It is shown, that such dangerous phenomena as degradation of permafrost and thermokarst would develop in the first place, which would cause the destruction of natural ecosystems.

The biomechanical study of lower limb during human walking

The coordinates of the marked points of joints and the changes of foot force in the human walk motion are obtained with systems of motion image acquisition and force measuring.The effects of kinematics and dynamics on the human lower limb in different loads(0,10,20,30 kg) and walking speeds(0.8,1.3,1.7 m/s) have been investigated using the human dynamics model,.The results were as follows.First,with the increasing load,the dorsiflexion angle of ankle joint increased during the procedure of toe-off,the range of motion of the knee joint decreased,the flexion angle of the hip joint increased,and oppositely,the extension angle of the hip joint decreased.Second,with the increasing speed,the range of motion of the ankle joint,the maximum flexion angle of the knee joint,the flexion angle,extension angle of the hip joint and the range of motion of hip joint increased.Finally,the torque and power of the ankle joint,the knee joint and the hip joint increased with the increasing speed and load.The results can provide an important basis for the mechanism design of lower limb power-assisted exoskeleton.

Ideas, properties, and standards of fracture repositioning with osteopathy in traditional Mongolian medicine in China

OBJECTIVE: To explore the unique ideas, properties, and standards of fracture repositioning with osteopathy in traditional Mongolian medicine in China.METHODS: Based on the natural life concept of "integration of universe and man," osteopathy in traditional Mongolian medicine in China uses the modern principles and methods of physiology, psychol-ogy, and biomechanics. Against this background,we explored the unique ideas, properties, and standards of fracture repositioning in traditional Mongolian medicine.RESULTS: Fracture treatment with osteopathy in traditional Mongolian medicine in China is based on(a) the ideas of natural, sealed, self and dynamic repositioning of fractures;(b) the properties of structural continuity and functional completeness;(c) the standards of "integration of movement and stillness" and "force to force." CONCLUSION: The unique ideas, properties, and standards of fracture repositioning with osteopathy in traditional Mongolian medicine in China have resulted in the widespread use of such techniques and represents the future direction of the development of fracture repositioning.

Effect of reinforcing kidney-essence, removing phlegm, and promoting mental therapy on treating Alzheimer's disease

OBJECTIVE: To explore the mechanism of reinforcing kidney-essence, removing phlegm, and promoting mental therapy in treating Alzheimer's disease (AD). METHODS: Sixty patients with AD in Wuhan No.1 Hospital of Traditional Chinese Medicine for Geriatrics from May 2009 to April 2011 were randomly divided into two groups, with 30 in each group. Patients in Bushenhuatanyizhi group (BHY group) took BHY instant granules (6 g, twice per day). Patients in the control group took piracetam (0.8 g, 3 times per day). There were twelve weeks in a course. Changes in the mini-mental state examination (MMSE) score and Activity of Daily Living Scale (ADL) score were analyzed before and after treatment. RESULTS: MMSE scores in the two groups in-creased and ADL scores decreased after treatment, compared with those before treatment (both P< 0.05).The total effective rate was 86.67% in the BHY group (10 very effective, 13 effective, 7 non-responsive). The control group was 57.69% (5 very effective, 16 effective, 9 non-responsive) (P<0.05). Superoxide dismutase levels were increased, lipid peroxide and triglyceride levels decreased after treatment in the BHY group as compared with the control group. CONCLUSION: Reinforcing kidney-essence, removing phlegm, and promoting mental therapy can improve cognitive function and daily life of AD patients. The mechanism of the therapy might be related to improving blood fat, scavenging free radicals, and inhibiting lipid peroxides.