[Objective] The paper was to explore the regularity between heat shock protein expression and the healthiness changes of Bombyx moil materials. [Method] The representative heat shock protein gene Bmhsp24.3 was screene...[Objective] The paper was to explore the regularity between heat shock protein expression and the healthiness changes of Bombyx moil materials. [Method] The representative heat shock protein gene Bmhsp24.3 was screened by bioinfor- matic analysis method, and carried out real-time PCR expression analysis. [Result] The target gene Bmhsp24.3 expressed in different B. mori materials, but the expres- sion level in different materials significantly varied. The relative expression level of the gene had different degrees of changes under different rearing conditions. With the increase of rearing temperature, the gene expression was upregulated. The ma- terials with better healthiness had remarkable increase in expression of target gene, while the materials with poorer healthiness had less increase in expression of target gene. The expression difference of target gene Bmhsp24.3 was exactly consistent with the healthiness of breeds. [Conclusion] The healthiness of materials had rela- tionship with expression of target gene Bmhsp24.3. the higher the expression of tar- get gene Bmhsp24.3 was, the better the healthiness of materials was; conversely, the lower the expression of target gene Bmhsp24.3 was, the poorer the healthiness of materials was.展开更多
AIM： To elucidate the role of Rab23 in hepatocellular carcinoma （HCC） by assessing the expression of Rab23 in HCC tissue and in HCC cell lines. METHODS： Primary tumors （n = 100） were stained with Rab23 antibodie...AIM： To elucidate the role of Rab23 in hepatocellular carcinoma （HCC） by assessing the expression of Rab23 in HCC tissue and in HCC cell lines. METHODS： Primary tumors （n = 100） were stained with Rab23 antibodies using immunohistochemistry and in situ hybridization in tissue microarrays. Relationships between gene expression and pathology parameters were analysed. The biological significance of Rab23 in Hep-3B cells was examined by knocking down Rab23 gene expression. We designed a pair of doublestranded RNAs against human rab23 and transfected siRNA into Hep-3B cells. Rab23 expression in these cells was examined using RT-PCR and Western blots. We investigated cell growth by MTT assays and fluorescenceactivated cell sorting. RESULTS： High cytoplasmic and nuclear expression of Rab23 was found in 38 of 71 （53.5%） and in 49 of 68 HCC patients （72%） respectively, which correlated with tumor size. HCC cell lines expressed Rab23. In Hep3B cells, siRNA for Rab23 decreased Rab23 mRNA by 4.5-fold and protein expression by 2-fold. Survival rates at 24 and 48 h for Hep-3B cells tTansfected with siRNA were lower and about 30% Hep-3B cells were apoptotic. Knocking down rab23 suppressed Hep3B cell growth, suggesting that rab23 could play an important role in Hep3B cell growth. CONCLUSION： Rab23 is overexpressed and/or activatedin HCC. Rab23 may be both a HCC predictor and a target for treating HCC.展开更多
[Objective] This research aimed at constructing two gateway-compatible plant expression vectors for functional genomics of abiotic stress in tomato.[Method] pK2GW7I was generated from the plant expression vector pK2GW...[Objective] This research aimed at constructing two gateway-compatible plant expression vectors for functional genomics of abiotic stress in tomato.[Method] pK2GW7I was generated from the plant expression vector pK2GW7,0 by replacing the CaMV 35S promoter with abiotic-stress inducible plant promoter pRD29A,which was derived from the promoter of Arabidopsis gene RD29A.pKGW121 was generated by replacing the CaMV 35S of the plant expression vector pRD410 with the gateway recombinant cassette(attR1-cmR-ccdB-attR2)from pK2GW7,0.Constitutive and root-specific promoters were tested on pKGW121.[Result] Two gateway-compatible destination vectors,pK2GW7I and pKGW121,were successfully constructed;practicability test proved that pKGW121 was applicable for promoter analysis.[Conclusion] With the incorporation of gateway cloning technology or abiotic-stress inducible promoter,pKGW121 and pK2GW7I will be promising in large-scale investigation of tomato functional genes associated with various abiotic stresses.A high-throughput workflow for the construction of plant transformation vector was proposed and validated.展开更多
The present study achieves the biochemistry partial characterization of the chitinolytic extract produced by an endophytic Streptomyces sp. strain （A8）. This extract was also tested against Anthonomus grandis, the c...The present study achieves the biochemistry partial characterization of the chitinolytic extract produced by an endophytic Streptomyces sp. strain （A8）. This extract was also tested against Anthonomus grandis, the cotton boll weevil aiming its control. The chitinase crude extract from the A8 strain was cultured for five days in a minimum liquid media supplemented with chitin. The extract was partially characterized by standard methods. The chitinolytic extract had an optimum temperature of 66 ＂C and an optimum pH between 4 to 9 （around 80% of relative activity）. We also characterized the temperature and pH stability and measured the effects of enzyme inhibitors. The filtered chitinolytic extract was added to an artificial boll weevil diet. Boll weevil development from the egg stage to the adult stage was prolonged, and the percentage of adults that emerged was approximately 66% less than on control diet. This study showed that the.larval development of A. grandis was inhibited by the presence of characterized chitinolytic extract in artificial diet. This work provides an experimental basis for using the chitinase from an endophytic bacterium Streptomyces sp. as a biocontrol alternativeto controlling the plant pest A. grandis.展开更多
Chaperonin-containing tailless complex polypeptide 1(CCT) is a group of genes involved in protein folding. It has been reported to be associated with the genesis and development of multiple tumors. However, the expres...Chaperonin-containing tailless complex polypeptide 1(CCT) is a group of genes involved in protein folding. It has been reported to be associated with the genesis and development of multiple tumors. However, the expression levels and functions of distinct CCTs involved in carcinogenesis and progression of hepatocellular carcinoma(HCC) have not been systematically analyzed. In the present study, we aimed to investigate the expression and mutation patterns, diagnostic and prognostic value, and functional enrichment of CCTs in HCC using ONCOMINE, GEPIA, the Human Protein Atlas, cBioPortal, Kaplan-Meier Plotter, and R language. The transcriptional and translational levels of all CCT family members were remarkably higher in HCC patients and related to the tumor stage. All CCT family members, especially CCT2 and CCT8, might serve as promising diagnostic, prognostic markers as well as therapeutic targets for HCC.展开更多
Natural products have been extensively used to treat diseases throughout human history. These are mainly because natural products normally target biological macromolecules selectively. Target identification could help...Natural products have been extensively used to treat diseases throughout human history. These are mainly because natural products normally target biological macromolecules selectively. Target identification could help us to develop new therapeutic agents and discover new biological pathways underlying human diseases. Herein, we highlight some recent examples of using natural products and their derivatives as chemical probes to identify the molecular targets and elucidate mode of action.展开更多
基金Supported by Youth Fund of Provincial Finance for Breeding Project(2010QNJJ-023)Fund Project of Agricultural Science and Technology Achievement Conversion(2010F00080)Science and Technology Supporting Project of Nanchong City(11A0016)~~
文摘[Objective] The paper was to explore the regularity between heat shock protein expression and the healthiness changes of Bombyx moil materials. [Method] The representative heat shock protein gene Bmhsp24.3 was screened by bioinfor- matic analysis method, and carried out real-time PCR expression analysis. [Result] The target gene Bmhsp24.3 expressed in different B. mori materials, but the expres- sion level in different materials significantly varied. The relative expression level of the gene had different degrees of changes under different rearing conditions. With the increase of rearing temperature, the gene expression was upregulated. The ma- terials with better healthiness had remarkable increase in expression of target gene, while the materials with poorer healthiness had less increase in expression of target gene. The expression difference of target gene Bmhsp24.3 was exactly consistent with the healthiness of breeds. [Conclusion] The healthiness of materials had rela- tionship with expression of target gene Bmhsp24.3. the higher the expression of tar- get gene Bmhsp24.3 was, the better the healthiness of materials was; conversely, the lower the expression of target gene Bmhsp24.3 was, the poorer the healthiness of materials was.
文摘AIM： To elucidate the role of Rab23 in hepatocellular carcinoma （HCC） by assessing the expression of Rab23 in HCC tissue and in HCC cell lines. METHODS： Primary tumors （n = 100） were stained with Rab23 antibodies using immunohistochemistry and in situ hybridization in tissue microarrays. Relationships between gene expression and pathology parameters were analysed. The biological significance of Rab23 in Hep-3B cells was examined by knocking down Rab23 gene expression. We designed a pair of doublestranded RNAs against human rab23 and transfected siRNA into Hep-3B cells. Rab23 expression in these cells was examined using RT-PCR and Western blots. We investigated cell growth by MTT assays and fluorescenceactivated cell sorting. RESULTS： High cytoplasmic and nuclear expression of Rab23 was found in 38 of 71 （53.5%） and in 49 of 68 HCC patients （72%） respectively, which correlated with tumor size. HCC cell lines expressed Rab23. In Hep3B cells, siRNA for Rab23 decreased Rab23 mRNA by 4.5-fold and protein expression by 2-fold. Survival rates at 24 and 48 h for Hep-3B cells tTansfected with siRNA were lower and about 30% Hep-3B cells were apoptotic. Knocking down rab23 suppressed Hep3B cell growth, suggesting that rab23 could play an important role in Hep3B cell growth. CONCLUSION： Rab23 is overexpressed and/or activatedin HCC. Rab23 may be both a HCC predictor and a target for treating HCC.
基金Supported by the Natural Science Foundation of China(30771461)the Wuhan Chenguang Program of Hubei Provicne of China(20055003059-24)
文摘[Objective] This research aimed at constructing two gateway-compatible plant expression vectors for functional genomics of abiotic stress in tomato.[Method] pK2GW7I was generated from the plant expression vector pK2GW7,0 by replacing the CaMV 35S promoter with abiotic-stress inducible plant promoter pRD29A,which was derived from the promoter of Arabidopsis gene RD29A.pKGW121 was generated by replacing the CaMV 35S of the plant expression vector pRD410 with the gateway recombinant cassette(attR1-cmR-ccdB-attR2)from pK2GW7,0.Constitutive and root-specific promoters were tested on pKGW121.[Result] Two gateway-compatible destination vectors,pK2GW7I and pKGW121,were successfully constructed;practicability test proved that pKGW121 was applicable for promoter analysis.[Conclusion] With the incorporation of gateway cloning technology or abiotic-stress inducible promoter,pKGW121 and pK2GW7I will be promising in large-scale investigation of tomato functional genes associated with various abiotic stresses.A high-throughput workflow for the construction of plant transformation vector was proposed and validated.
文摘The present study achieves the biochemistry partial characterization of the chitinolytic extract produced by an endophytic Streptomyces sp. strain （A8）. This extract was also tested against Anthonomus grandis, the cotton boll weevil aiming its control. The chitinase crude extract from the A8 strain was cultured for five days in a minimum liquid media supplemented with chitin. The extract was partially characterized by standard methods. The chitinolytic extract had an optimum temperature of 66 ＂C and an optimum pH between 4 to 9 （around 80% of relative activity）. We also characterized the temperature and pH stability and measured the effects of enzyme inhibitors. The filtered chitinolytic extract was added to an artificial boll weevil diet. Boll weevil development from the egg stage to the adult stage was prolonged, and the percentage of adults that emerged was approximately 66% less than on control diet. This study showed that the.larval development of A. grandis was inhibited by the presence of characterized chitinolytic extract in artificial diet. This work provides an experimental basis for using the chitinase from an endophytic bacterium Streptomyces sp. as a biocontrol alternativeto controlling the plant pest A. grandis.
基金National Natural Science Foundation of China (Gr ant No. 81803535)Natural Science Foundation of Hunan Province of China (Grant No. 2018JJ6070)。
文摘Chaperonin-containing tailless complex polypeptide 1(CCT) is a group of genes involved in protein folding. It has been reported to be associated with the genesis and development of multiple tumors. However, the expression levels and functions of distinct CCTs involved in carcinogenesis and progression of hepatocellular carcinoma(HCC) have not been systematically analyzed. In the present study, we aimed to investigate the expression and mutation patterns, diagnostic and prognostic value, and functional enrichment of CCTs in HCC using ONCOMINE, GEPIA, the Human Protein Atlas, cBioPortal, Kaplan-Meier Plotter, and R language. The transcriptional and translational levels of all CCT family members were remarkably higher in HCC patients and related to the tumor stage. All CCT family members, especially CCT2 and CCT8, might serve as promising diagnostic, prognostic markers as well as therapeutic targets for HCC.
基金supported by the National Basic Research Program of China (2015CB856200)the National Natural Science Foundation of China (21472010, 21561142002)
文摘Natural products have been extensively used to treat diseases throughout human history. These are mainly because natural products normally target biological macromolecules selectively. Target identification could help us to develop new therapeutic agents and discover new biological pathways underlying human diseases. Herein, we highlight some recent examples of using natural products and their derivatives as chemical probes to identify the molecular targets and elucidate mode of action.
