微波消解法测定胎儿大脑组织中生物必需元素

目的 建立胎儿大脑组织中生物必需元素K、Na、Mg、P、Cu、Fe、Mn、Zn的分析方法。方法 样品经HNO3 +H2 O2 微波消解体系消解后 ,用电感耦合等离子体发射光谱法 (ICP -AES)同时测定K、Na、Mg、P、Cu、Fe、Mn、Zn含量。结果 元素的检出限分别在 0 0 0 7mg/L～ 0 1mg/L之间 ;线性良好 ,线性相关系统均≥ 0 999;重现性好 ,变异系统RSD <2 % ;准确度高 ,回收率在 93 7%～ 16 1%之间 ,用猪肝 (GBW 0 8551)及人发 (GBW 0 760 1)标样质控 ,分析结果与标准值吻合。结论 本法快速灵敏 ,精密度及准确度均符合要求 。

胎儿小脑组织中Cu、Fe、Mn、Zn的测定研究

目的 建立胎儿小脑组织中生物必需微量元素Cu、Fe、Mn、Zn的分析方法。方法 样品经HNO3 +H2 O2 微波消解体系消解后 ,用电感耦合等离子体发射光谱法ICP -AES ,同时测定Cu、Fe、Mn、Zn含量。结果 元素的检出限分别在 0 0 0 0 7mg/L～ 0 0 3mg/L之间 ;线性良好 ,线性相关系数均≥ 0 999;重现性好 ,变异系数RSD >2 % ;准确度高 ,回收率在 93 7%～ 116 1%之间 ,用猪肝GBW0 85 5 1及人发GBW0 76 0 1标样质控 ,分析结果与标准值吻合。样品分析结果显示Cu、Mn呈正态分布 ,Fe、Zn呈正偏态分布。结论 本法快速灵敏 ,精密度及准确度均符合要求 。

Investigation of Essential or Non-essential Character of Some Hardly Known Trace Elements for Plants

The concentration distribution of essential and non-essential micro-elements of the tissues of healthy organisms shows significant difference. This fact can be used for clarification of the biological role of hardly known trace elements, as well, proving the essential or non-essential character. In case of essential elements the concentration range is rather narrow, the distribution is normal. In case of non-essential elements the interval is wide, the concentration-distribution is log-normal. Measurements and mathematical calculations were carried out for determination of the concentrations and concentration-distributions of some micro-elements, trying to clarify the essentiality or non-essential character of the investigated elements from point of view of plant physiology. Based on the measurements for radish Li and Ni seem to be non-essential trace elements, however in case of Sr the distribution curve suggests an essential character, because the concentration range is not wide.