球孢白僵菌对油桐尺蛾的生物效力(英文)

分别在2005年和2006年的5、6月份,在Kamalpur和Hunterpara茶园、孟加拉西部以及印度地区对一种昆虫致病真菌球孢白僵菌对油桐尺蛾Buzura(=Biston)suppressaria Guen.的生物效力进行了评估。以农场主常用的化学杀虫剂40%SP灭多虫和25%EC氯氰菊酯作为标准检测物。试验中球孢白僵菌浓度为1.50 g,2.00 g和2.5 g/lit.of water;灭多虫和氯氰菊酯浓度分别为0.75 mL,1.00 mL和1.50 mL,和0.50 mL,1.00 mL和1.50 mL/lit.of water。在喷洒2.5 g/lit.of water球孢白僵菌3天后,Kamalpur和Hunterpara茶园中油桐尺蛾种群个体数分别降低了88.00%和84.00%。死的毛虫变成黑色,垂悬在叶片上。并且发现球孢白僵菌的杀虫活性与高剂量的灭多虫和氯氰菊酯的相当。

Response Ability to External Signal Enhanced by Biological Spatial Configuration in Coupled Hindmarsh-Rose Neural System

The effect of realistic topology configuration of intercellular connections on the response ability in coupled cell system is numerically investigated by using the Hindmarsh-Rose model. For the proper coupling intensity, we set the control parameter to be near the critical value, and the external stimulus is introduced to the first cell in coupled system. It is found that, on one hand, when the cells are coupled with some proper topological structures, the external stimulus could transmit through the system, and shows better response ability and higher sensitivity. On the other hand, the influence of topological configuration on the synchronous ability and selection effect of neural system are also discussed. Our results display that the topology of coupled system may play an important role in the process of signal propagation, which could help us to understand the coordinated performance of cells in tissue.

Estimation of Net Primary Productivity of Terrestrial Vegetation in China by Remote Sensing

Among the many approaches for studying the net primary productivity (NPP), a new method by using remote sensing was introduced in this paper. With spectral information source (the visible band, near infrared band and thermal infrared band) of NOAA-AVHRR, we can get the relative index and parameters, which can be used for estimating NPP of terrestrial vegetation. By means of remote sensing, the estimation of biomass and NPP is mainly based on the models of light energy utilization. In other words, the biomass and NPP can be calculated from the relation among NPP, absorbed photosynthetical active radiation (APAR) and the rate (epsilon) of transformation of APAR to organic matter, thus: NPP = ( FPAR x PAR) x [epsilon * x sigma (T) x sigma (E) x sigma (S) x (1 - Y-m) x (1 - Y-g)]. Based upon remote sensing ( RS) and geographic information system (GIS), the NPP of terrestrial vegetation in China in every ten days was calculated, and the annual NPP was integrated. The result showed that the total NPP of terrestrial vegetation in China was 6.13 x 10(9) t C . a(-1) in 1990 and the maximum NPP was 1 812.9 g C/m(2). According to this result, the spatio-temporal distribution of NPP was analyzed. Comparing to the statistical models, the RS model, using area object other than point one, can better reflect the distribution of NPP, and match the geographic distribution of vegetation in China.

Pharmacokinetics and bioequivalence of ranitidine and bismuth derived from two compound preparations

AIM： To evaluate the bioequivalence of ranitidine and bismuth derived from two compound preparations. METHODS： The bioavailability was measured in 20 healthy male Chinese volunteers following a single oral dose （equivalent to 200 mg of ranitidine and 220 mg of bismuth） of the test or reference products in the fasting state. Then blood samples were collected for 24 h. Plasma concentrations of ranitidine and bismuth were analyzed by high-performance liquid chromatography and inductively coupled plasma-mass spectrometry （ICPMS）, respectively. The non-compartmental method was used for pharmacokinetic analysis. Log-transformed Cmax, AUC（0-t） and AUC（0-∞） were tested for bioequivalence using ANOVA and Schuirmann two-one sided t-test. Tmax was analyzed by Wilcoxon＇s test. RESULTS： Various pharmacokinetic parameters of ranitidine derived from the two compound preparations, including Cmax, AUC（0-t）, AUC（0-∞）, Tmax and T1/2, were nearly consistent with previous observations. These parameters derived from test and reference drug were as follows： Cmax（0.67 ± 0.21 vs 0.68 ± 0.22 mg/L）, AUC（0-t）（3.1 ± 0.6 vs 3.0 ± 0.7 mg/L per hour）, AUC（0-∞）（3.3 ± 0.6 vs 3.2 ± 0.8 mg/L per hour）, Tmax （2.3 ± 0.9 VS 2.1 ± 0.9 h） and T1/2 （2.8 ± 0.3 vs 3.1 ± 0.4 h）. In addition, double-peak absorption profiles of ranitidine were found in some Chinese volunteers. For bismuth, those parameters derived from test and reference drug were as follows： Cmax （11.80 ± 7.36 vs 11.40 ± 6.55 μg/L）, AUC（0-t） （46.65 ± 16.97 vs 47.03 ± 21.49 μg/L per hour）, Tmax （0.50 ± 0.20 vs 0.50 ± 0.20 h） and T1/2 （10.2 ± 2.3 vs 13.0 ± 6.9 h）. Ninety percent of confidence intervals for the test/reference ratio of Cmax, AUC（0-t） and AUC（0-∞） derived from both ranitidine and bismuth were found within the bioequivalence acceptable range of 80%-125%. No significant difference was found in Tmax derived from both ranitidine and bismuth. CONCLUSION： The two compound preparations are bioequivalent and may be prescribed interchangeably.

Pharmacokinetic Study of a Novel Recombinant Human Granulocyte Colony-stimulating Factor in Rats

Objective To study the pharmacokinetics of a novel recombinant human granulocyte colonystimulating factor (rhG-CSFa) in rats and to determine the proteolytic rates of rhG-CSFa in the whole blood and serum of rats in vitro. Methods The pharmacokinetics of rhG-CSFa and conventional (wild type,WT) granulocyte colonystimulating factor (G-CSF) were investigated in Sprague-Dawley rats which received either intravenous or subcutaneous injection of rhG-CSFa or WT G-CSF at three different doses (20,50,or 100 μg/kg). The blood samples of rats were collected at multiple time points (from 0.08 to 12 h) and the concentrations of rhG-CSFa and WT G-CSF in serum were determined with a sandwich enzyme-linked immunosorbent assay (ELISA). For the study of proteolytic rates in vitro,the concentrations of rhG-CSFa or WT G-CSF were determined at 3-minute intervals after addition of the respective drug to rat’s whole blood or serum. Results Pharmacokinetic analysis of serum rhG-CSFa or WT G-CSF levels indicated that,at each dose tested,for either route of drug administration,the area under concentration-time curve values and the maximum serum concentration of rhG-CSFa were higher than those of WT G-CSF,and the serum half life of rhG-CSFa was longer than that of WT G-CSF. Subsequent in vitro whole blood and serum stability study showed that the rates of drug degradation in WT G-CSF were 1.8 folds and 1.5 folds higher than those in rhG-CSFa,respectively. Conclusion rhG-CSFa has better serum and whole blood stability in vitro and higher bioavailability in vivo as compared to WT G-CSF.

Addition of Urban Waste to Semiarid Degraded Soil:Long-term Effect

The addition of municipal solid wastes （MSW） is considered as a possible strategy for soil rehabilitation in southeast Spain. The objective of this study was to evaluate the long-term （17 years） effect of five doses of MSW addition on the microbiological, biochemical, and physical properties of semiarid soil. Increased values of several parameters that serve as indicators of general microbiological activity, such as, basal respiration, adenosine triphosphate （ATP） or dehydrogenase activity; microbial population size （microbial biomass C）, and extracellular hydrolase activity related to macronutrient cycles, such as, urease, 3-glucosidase, and N-a-benzoyl-L-argininamide protease, were observed in the amended soils. The highest MSW doses showed the highest values in these hydrolase activities. The incorporation of municipal waste resulted in a more dense development of the plant cover, 50% greater in higher doses than in the control treatment, which generated a substantial increase in several C fractions. Total organic carbon reached 12 g kg^-1 soil with the highest MSW doses, compared to 4.30 g kg^-1 soil in the control treatment. The physical properties of the soil were also improved, showing greater percentage of stable aggregates and water holding capacity. Positive correlation coefficients between C fractions and parameters related to microbial activity and aggregate stability were observed. Although these improvements were greater in the soils receiving the highest doses of organic amendment, the increases were not proportional to the amount added, demonstrating the existence of a threshold, above which an increase in the amount of organic matter added is not reflected in an increase in the soil＇s physical, biochemical, and microbiological properties. However, the addition of municipal solid wastes proved its suitability for improving soil quality, thereby indicating the potential of such an amendment, to prevent desertification in Mediterranean areas such as those studied.

Effects of improved fallow with Sesbania sesban on maize productivity and Striga hermonthica infestation in Western Kenya

Striga hermonthica is a major constraint to smallholder sub-sistence agriculture production in the sub-Saharan African region. Low soil fertility and overall environmental degradation has contributed to the build-up of the parasitic weed infestation. Improved cropping systems have to be introduced to address the interrelated problems of S. her-monthica and soil fertility decline. Thus, the effects of improved fallow with leguminous shrub Sesbania sesban on maize yields and levels of S. hermonthica infestation on farm land in the bimodal highlands of western Kenya were investigated. The experimental treatments were arranged in a phased entry, and randomized complete block scheme were six months Sesbania fallow, 18 months Sesbania fallow, six months natural fallow consisting of regrowth of natural vegetation without cultivation, 18 months natural fallow, continuous maize cropping without fertilizer application, and continuous maize cropping with P and N fertilization. Results show that Sesbania fallows significantly （p0.05） increase maize yield relative to continuous unfertilized maize. S. hermonthica plant populations decrease in continuous maize between the first season （mean = 428 000 ± 63 000 ha-1） and second season （mean=51 000 ± 15 000 ha-1）, presumably in response to good weed management. S. hermonthica seed populations in the soil decrease throughout the duration of the experi-ment in the continuous maize treatments. Short-duration Sesbania fal-lows can provide modest yield improvements relative to continuous unfertilized maize, but short-duration weedy fallows are ineffective. Continuous maize cultivation with good weed control may provide more effective S. hermonthica control than fallowing.

Non-Isothermal Desolvation Kinetics of Erythromycin A Acetone Solvate

The desolvation of erythromycin acetone solvate was investigated under non-isothermal conditions by a thermogravimetric analyzer. This paper emphasized the kinetic analysis of non-isothermal TG-DTA data by Achar method and Coats-Redfern method to fit various solid-state reaction models, and to achieve kinetic parameters of desolvation. The mechanism of thermal desolvation was evaluated using the kinetic compensation effect. The results show that kinetics of desolvation of erythromycin acetone solvate was compatible with the mechanism of a two-dimensional diffusion controlled and was best expressed by Valensi equation. Corresponding to the integral method and the differential method, the activation energy of desolvation of erythromycin acetone solvate was estimated to be 51.26—57.11 kJ/mol, and the pre-exponential factor was 8.077×106 s-1—4.326×107 s-1, respectively.

硒元素在动物体内的吸收代谢研究进展

硒是动物必需的营养矿物质,具有抗氧化、提高免疫力、预防疾病等重要生理功能.文章从硒活性形式和转运方式、代谢模式、吸收器官和体内分布、排泄途径及生物效力影响因素等方面对硒元素在动物体内的吸收代谢研究进展进行了综述,并对硒元素在产业中的应用进行了展望.

重组糖蛋白激素类药物的质量控制和评价关注点:糖基化修饰的“宏观”和“微观”异质性

糖蛋白激素包括促卵泡激素、促黄体生成素、绒毛膜促性腺激素和促甲状腺激素,是由非共价结合的α亚基和β亚基组成的异源二聚体。糖基化复杂性和结构不均一性是糖蛋白激素类药物结构表征和质量控制的关注点。糖基化修饰位点、修饰类型、唾液酸含量与糖蛋白激素在机体的受体结合、信号转导、生物功能和清除速率密切相关。本文将梳理重组糖蛋白激素类药物的上市产品概况,解析该类药物糖基化修饰的“宏观”和“微观”复杂性,阐明结构异质性与生物功能的关系,梳理用于结构确证和糖链鉴定的分析技术,结合其质量控制难点提出研发路径和药学研究的相关考虑。

The clinical pharmacokinetics of osmotic pump controlled release tablets of terazosin hydrochloride in healthy volunteers

The clinical pharmacokinetics of osmotic pump controlled release tablets of terazosin hydrochloride in healthy volunteers was studied.A sensitive and rapid HPLC method was used to determine the terazosin plasma concentrations,and single and multiple doses of terazosin hydrochloride regular tablets（reference tablets）and osmotic pump controlled release tablets were orally administrated in randomized crossover design.The results showed that the C_（max）of the reference tablets after single oral dose（（120.56±23.15）ng/mL）in 20 healthy volunteers was significantly higher than that of controlled release tablets （（95.27±16.35）ng/mL）.The T_（max）of the controlled release tablets（（2.65±0.82）h）was significantly longer than that of reference tablets（（1.27±0.61）h）（P0.05）.The relative bioavailability of the controlled release tablets was found to be（105.85±6.12）%. The multiple oral dose pharmacokinetic parameters of the regular tablets and controlled release tablets were as follows：AUC_（SS） were（1275.17±175.35）and（1382.65±205.31）ng·h/mL respectively,C_（max）were（128.15±22.37）and（98.57±18.16）ng/mL respectively,T_（max）were（1.35±0.71）and（2.76±0.85）h respectively,C_（av）were（53.13±9.12）and（57.61±9.25）ng/mL respectively, and DF were（2.25±0.26）%and（1.62±0.25）%respectively.The relative bioavailability of the controlled release tablets to the reference tablets was（108.43±6.26）%.The controlled release tablet of terazosin hydrochloride was bioequivalent to the reference tablet.The controlled release tablet exhibited a sustained-release property with a significantly longer T_（max）and lower C_（max）.

Advances in environmental behaviors and effects of dissolved organic matter in aquatic ecosystems

Dissolved organic matter(DOM) is a group of compounds that have complex chemical structures and multiple interactions with their surrounding materials. More than one trillion tons of DOM are stocked in the world's aquatic ecosystems. DOM is a very important part of aquatic ecosystem productivity and plays a crucial role in global carbon cycling. DOM has rich environmental behaviors and effects such as influencing the bioavailability of contaminants, serving as an important inducer of reactive oxygen species(ROS), and protecting aquatic organisms from the harm of dangerous ultraviolet radiation. There have been many systematic studies on the composition, structure, and sources of DOM because such studies are much easier to conduct than studies on the environmental behaviors and effects of DOM. Due to many factors, the research systems of DOM's environmental behaviors and effects are still being developed and have become a hotspot of environmental science. This review paper focuses on some critical progress, problems, and trends of DOM's environmental behaviors and effects in aquatic ecosystems, including mutual exchange mechanisms between DOM and particulate organic matter(POM) with influencing factors, photochemical behaviors of DOM especially inducing ROS, binding interactions between DOM and anthropogenic organic contaminants(AOC), interactions between DOM and microorganisms, effects of DOM on pollutants' bioavailability, ecotoxicity, and ecological risks. Hopefully, this paper will contribute to a more systematic understanding of the DOM environmental behaviors and effects and to promoting further relevant studies.

Relative abundance of invasive plants more effectively explains the response of wetland communities to different invasion degrees than phylogenetic evenness

Native plant communities are commonly invaded by invasive plants to different degrees.However,the relative contribution of the invasive plant abundance vs.phylogenetic evenness to the responses of wetland communities to different degrees of invasion is still unclear.In addition,whether such contribution varies with environmental conditions such as flooding is also unclear.To address these questions,we chose Alternanthera philoxeroides as the invasive plant,and set up four invasive degrees by changing the community species composition under both flooding and non-flooding conditions.The relative abundance of A.philoxeroides and phylogenetic evenness changed simultaneously with the change in the community invasion degree.The invasion degree significantly affected the individual biomass of A.philoxeroides and some native species.Variation partitioning showed that the relative abundance of A.philoxeroides contributed more to variation in community indicators than phylogenetic evenness,regardless of flooding.Spearman rank correlation test showed that the relative abundance of A.philoxeroides was negatively correlated with the individual biomass of A.philoxeroides and some native species,while the phylogenetic evenness was positively correlated with only a few native species.And their correlation strength and significance were all affected by specific species and flooded environment.In conclusion,our results suggest that the relative abundance of A.philoxeroides can more effectively explain the wetland community response to different invasion degrees than phylogenetic evenness,regardless of flooding.