蛋白质组学是基因组时代产生的一门重要学科,是从整体水平对蛋白质的综合分析。阿尔采末病(A lzheimer's d isease,AD)是常见而复杂的神经退行性疾病之一。应用蛋白质组学对AD进行研究,不仅可在蛋白质水平上揭示疾病的本质,还有助...蛋白质组学是基因组时代产生的一门重要学科,是从整体水平对蛋白质的综合分析。阿尔采末病(A lzheimer's d isease,AD)是常见而复杂的神经退行性疾病之一。应用蛋白质组学对AD进行研究,不仅可在蛋白质水平上揭示疾病的本质,还有助于全面探讨AD的病理机制,建立诊断标准,发现药物治疗靶点。本文从病理机制(特别是蛋白质翻译后修饰)、发现临床生物标签及治疗药物三个方面,对蛋白质组学在AD中的研究进展进行了综述。展开更多
Molecular biomarkers are the important maturity parameters for sedimentary organic matter. They have also been widely used for determining the maturity of organic matter in ore deposits. However, during the study of o...Molecular biomarkers are the important maturity parameters for sedimentary organic matter. They have also been widely used for determining the maturity of organic matter in ore deposits. However, during the study of organic matter in the Kupferschiefer from the Lubin mine, it had been found that the biomarkers were influenced by sulfide formation. In order to probe into the degree of influence on biomarkers, seven samples collected from a Kupferschiefer section from the Lubin mine were analyzed by various geochemical methods. The results indicated that in the samples with higher copper contents, the values of biomarkers are lower than in the samples with lower copper contents. In highly mineralized samples, hydrogen donation for thermochemical sulfate reduction (TSR) occurred in alkylated phenanthrenes and naphthalenes, leading to the decrease of 12 biomarker parameters during the Kupferschiefer mineralization.展开更多
Abstract： The objective of this study was to provide insights on the motivations behind the decision of biodynamic producers in the United States to shift to biodynamic practices in the vineyard and winemaking proces...Abstract： The objective of this study was to provide insights on the motivations behind the decision of biodynamic producers in the United States to shift to biodynamic practices in the vineyard and winemaking process. The second objective of the study was to evaluate the perceptions of the consumers regarding eco-label wines, with a direct focus on the attitudes of millennial generation. Two surveys, one directed to biodynamic producers in the United States and the other to a group of Californian wine consumers were distributed during spring and fall of 2010. Findings from the first survey reveal that biodynamic producers are engaged in biodynamic practices out of socially motivated reasons rather than economic factors. Results from the consumer survey suggest a generally uninformed generation of consumers about eco-label wines and particularly about the practices associated with these products. This lack of information heavily influences their behavioral decisions and attitudes and points out to the critical need for consumer education.展开更多
Nanoparticles have enormous potential for bioimaging and biolabeling applications, in which conventional organically based fluorescent labels degrade and fail to provide long-term tracking. Thus, the development of ap...Nanoparticles have enormous potential for bioimaging and biolabeling applications, in which conventional organically based fluorescent labels degrade and fail to provide long-term tracking. Thus, the development of approaches to make fluorescent probes water soluble and label cells efficient is desirable for most biological applications. Here, we report on the fabrication and charac- terization of self-assembled nanodots （SANDs） from 3-aminopropyltriethoxysilane （APTES） as a probe for protein labeling. We show that fluorescent SAND probes exhibit both bright photoluminescence and biocompatibility in an aqueous environment. Selective in vitro imaging using protein and carbohydrate labeling of hepatoma cell lines are demonstrated using biocompatible SANDs conjugated with avidin and galactose, respectively. Cytotoxicity tests show that conjugated SAND particles have negligible effects on cell proliferation. Unlike other synthetic systems that require multistep treatments to achieve robust surface functionalization and to develop flexible bioconjugation strategies, our results demonstrate the versatility of this one-step SAND fabrication method for creating multicolor fluorescent probes with the tailored functionalities, effident emission, as well as excellent biocompatibility, required for broad biological use.展开更多
基金Supported by National Natural Science Foundation ofChina(No.31070698)Shanghai Key Projects of Basic Research(No.10JC1400300)Innovation Foundation of Donghua University for PhD Candidates(No.12D11320)~~
文摘蛋白质组学是基因组时代产生的一门重要学科,是从整体水平对蛋白质的综合分析。阿尔采末病(A lzheimer's d isease,AD)是常见而复杂的神经退行性疾病之一。应用蛋白质组学对AD进行研究,不仅可在蛋白质水平上揭示疾病的本质,还有助于全面探讨AD的病理机制,建立诊断标准,发现药物治疗靶点。本文从病理机制(特别是蛋白质翻译后修饰)、发现临床生物标签及治疗药物三个方面,对蛋白质组学在AD中的研究进展进行了综述。
文摘Molecular biomarkers are the important maturity parameters for sedimentary organic matter. They have also been widely used for determining the maturity of organic matter in ore deposits. However, during the study of organic matter in the Kupferschiefer from the Lubin mine, it had been found that the biomarkers were influenced by sulfide formation. In order to probe into the degree of influence on biomarkers, seven samples collected from a Kupferschiefer section from the Lubin mine were analyzed by various geochemical methods. The results indicated that in the samples with higher copper contents, the values of biomarkers are lower than in the samples with lower copper contents. In highly mineralized samples, hydrogen donation for thermochemical sulfate reduction (TSR) occurred in alkylated phenanthrenes and naphthalenes, leading to the decrease of 12 biomarker parameters during the Kupferschiefer mineralization.
文摘Abstract： The objective of this study was to provide insights on the motivations behind the decision of biodynamic producers in the United States to shift to biodynamic practices in the vineyard and winemaking process. The second objective of the study was to evaluate the perceptions of the consumers regarding eco-label wines, with a direct focus on the attitudes of millennial generation. Two surveys, one directed to biodynamic producers in the United States and the other to a group of Californian wine consumers were distributed during spring and fall of 2010. Findings from the first survey reveal that biodynamic producers are engaged in biodynamic practices out of socially motivated reasons rather than economic factors. Results from the consumer survey suggest a generally uninformed generation of consumers about eco-label wines and particularly about the practices associated with these products. This lack of information heavily influences their behavioral decisions and attitudes and points out to the critical need for consumer education.
文摘Nanoparticles have enormous potential for bioimaging and biolabeling applications, in which conventional organically based fluorescent labels degrade and fail to provide long-term tracking. Thus, the development of approaches to make fluorescent probes water soluble and label cells efficient is desirable for most biological applications. Here, we report on the fabrication and charac- terization of self-assembled nanodots （SANDs） from 3-aminopropyltriethoxysilane （APTES） as a probe for protein labeling. We show that fluorescent SAND probes exhibit both bright photoluminescence and biocompatibility in an aqueous environment. Selective in vitro imaging using protein and carbohydrate labeling of hepatoma cell lines are demonstrated using biocompatible SANDs conjugated with avidin and galactose, respectively. Cytotoxicity tests show that conjugated SAND particles have negligible effects on cell proliferation. Unlike other synthetic systems that require multistep treatments to achieve robust surface functionalization and to develop flexible bioconjugation strategies, our results demonstrate the versatility of this one-step SAND fabrication method for creating multicolor fluorescent probes with the tailored functionalities, effident emission, as well as excellent biocompatibility, required for broad biological use.
