Bioactive scaffolds with interconnected porous structures are essential for guiding cell growth and new bone formation. In this work, we successfully fabricated three-dimensional （3D） porous β-tricalcium phosphate...Bioactive scaffolds with interconnected porous structures are essential for guiding cell growth and new bone formation. In this work, we successfully fabricated three-dimensional （3D） porous β-tricalcium phosphate （β-TCP）/calcium silicate （CS） composite scaffolds with different ratios by 3D printing technique and further investigated the physiochemical properties, in vitro apatite mineralization properties and degradability of porous β-TCP/CS scaffolds. Moreover, a series of in vitro cell experiments including the attachment, proliferation and osteogenic differentiation of mouse bone marrow stromal cells were conducted to testify their biological performances. The results showed that 3D printed β-TCP/CS scaffolds possessed of controllable internal porous structures and external shape. Furthermore, the introduction of CS decreased the shrinkage of scaffolds and improved the in vitro apatite formation activity and degradation rate. Meanwhile, compared with pure β- TCP scaffold, the β-TCP/CS composite scaffolds were more conducive to promote cell adhesion, spread and osteogenesis differentiation. However, when the content of CS was increased to 45%, the ions dissolution rate of the composite scaffolds was so high that leaded to the increase in pH value, which inhibited the proliferation of cells. Our results suggested that the introduction of appropriate CS into β-TCP bioceramic is an effective strategy to prepare bioactive 3D printed bioceramic scaffolds for hard tissue regeneration.展开更多
Biomimetic scaffolds are appealing products for the repair of bone defects using tissue engineering strategies.In the present study,novel biomimetic composite scaffolds,with similar properties to natural bone,were pre...Biomimetic scaffolds are appealing products for the repair of bone defects using tissue engineering strategies.In the present study,novel biomimetic composite scaffolds,with similar properties to natural bone,were prepared,blended and cross-linked with bioactive glass,type I collagen and phosphatidylserine.When exposed to cell culture solution in the absence of a cellular source,the composite scaffolds form crystals with octahedral structure.These crystals are similar to the products derived from MC3T3-E1 cell mineralization within the composite scaffolds,with respect to both composition and morphology.Furthermore,crystals with octahedral structure were observed to develop into plate-like hydroxyapatite.The bio-mineralization behavior of the composite scaffolds is likely influenced by inorganic components.Finally,a rabbit tibia defect model shows that the highly bioactive properties of the investigated composites result in excellent bone repair.展开更多
文摘Bioactive scaffolds with interconnected porous structures are essential for guiding cell growth and new bone formation. In this work, we successfully fabricated three-dimensional （3D） porous β-tricalcium phosphate （β-TCP）/calcium silicate （CS） composite scaffolds with different ratios by 3D printing technique and further investigated the physiochemical properties, in vitro apatite mineralization properties and degradability of porous β-TCP/CS scaffolds. Moreover, a series of in vitro cell experiments including the attachment, proliferation and osteogenic differentiation of mouse bone marrow stromal cells were conducted to testify their biological performances. The results showed that 3D printed β-TCP/CS scaffolds possessed of controllable internal porous structures and external shape. Furthermore, the introduction of CS decreased the shrinkage of scaffolds and improved the in vitro apatite formation activity and degradation rate. Meanwhile, compared with pure β- TCP scaffold, the β-TCP/CS composite scaffolds were more conducive to promote cell adhesion, spread and osteogenesis differentiation. However, when the content of CS was increased to 45%, the ions dissolution rate of the composite scaffolds was so high that leaded to the increase in pH value, which inhibited the proliferation of cells. Our results suggested that the introduction of appropriate CS into β-TCP bioceramic is an effective strategy to prepare bioactive 3D printed bioceramic scaffolds for hard tissue regeneration.
基金supported by the Science and Technology Plan Project of Fujian Department of Education,China (Grant No. JK2009021)
文摘Biomimetic scaffolds are appealing products for the repair of bone defects using tissue engineering strategies.In the present study,novel biomimetic composite scaffolds,with similar properties to natural bone,were prepared,blended and cross-linked with bioactive glass,type I collagen and phosphatidylserine.When exposed to cell culture solution in the absence of a cellular source,the composite scaffolds form crystals with octahedral structure.These crystals are similar to the products derived from MC3T3-E1 cell mineralization within the composite scaffolds,with respect to both composition and morphology.Furthermore,crystals with octahedral structure were observed to develop into plate-like hydroxyapatite.The bio-mineralization behavior of the composite scaffolds is likely influenced by inorganic components.Finally,a rabbit tibia defect model shows that the highly bioactive properties of the investigated composites result in excellent bone repair.
