通过脂质体介导基因转染的方法,获得了稳定携带TFAR19基因的小鼠红白血病细胞系MEL-TF19.经 Western blot证实该细胞系有 TFAR19蛋白表达.生长曲线及流式细胞仪检测结果表明,转染TFAR19基因后MEL细胞生长受到抑制,并且在凋亡诱因的存在...通过脂质体介导基因转染的方法,获得了稳定携带TFAR19基因的小鼠红白血病细胞系MEL-TF19.经 Western blot证实该细胞系有 TFAR19蛋白表达.生长曲线及流式细胞仪检测结果表明,转染TFAR19基因后MEL细胞生长受到抑制,并且在凋亡诱因的存在下使细胞凋亡率增高.流变学研究结果显示,转染TFAR19基因在加速MEL细胞凋亡的同时,使细胞对低渗的抵抗力降低,渗透脆性加大,细胞表面电荷减少,电泳率降低;与细胞最大变形能力呈负相关的弹性模量K1;增加,K2无明显变化,μ明显减小.以上结论从生物流变学角度为全面、深入地认识凋亡新相关基因TFAR19的功能提供了理论基础.展开更多
Herein the biowaste by-product spent coffee grounds(SCGs)from coffee industry were incorporated into asphalt binders for performance enhancement.From the analysis of Fourier transform infrared spectroscopy(FTIR),diffe...Herein the biowaste by-product spent coffee grounds(SCGs)from coffee industry were incorporated into asphalt binders for performance enhancement.From the analysis of Fourier transform infrared spectroscopy(FTIR),differential scanning calorimetry(DSC),dynamic shear rheometer(DSR),and Brookfield viscosity rheometer,it is confirmed that SCGs have potential prospects as bio-waste modifiers in the application of sustainable pavements.Results demonstrated that the modification process was mainly based on physical reinforcement.Compared with that of the neat asphalt,the shearing stress-resistant ability and high-temperature performance of the SCGs modified binders with the appropriate addition presented a bit of improvement;whereas the binders with 1%and 3%SCGs exhibited remarkably enhanced low-temperature stability.However,notable weaknesses of practical performance were shown for the binder with excessive content of SCGs,indicating the necessity of proportion selecting before application.展开更多
文摘通过脂质体介导基因转染的方法,获得了稳定携带TFAR19基因的小鼠红白血病细胞系MEL-TF19.经 Western blot证实该细胞系有 TFAR19蛋白表达.生长曲线及流式细胞仪检测结果表明,转染TFAR19基因后MEL细胞生长受到抑制,并且在凋亡诱因的存在下使细胞凋亡率增高.流变学研究结果显示,转染TFAR19基因在加速MEL细胞凋亡的同时,使细胞对低渗的抵抗力降低,渗透脆性加大,细胞表面电荷减少,电泳率降低;与细胞最大变形能力呈负相关的弹性模量K1;增加,K2无明显变化,μ明显减小.以上结论从生物流变学角度为全面、深入地认识凋亡新相关基因TFAR19的功能提供了理论基础.
文摘Herein the biowaste by-product spent coffee grounds(SCGs)from coffee industry were incorporated into asphalt binders for performance enhancement.From the analysis of Fourier transform infrared spectroscopy(FTIR),differential scanning calorimetry(DSC),dynamic shear rheometer(DSR),and Brookfield viscosity rheometer,it is confirmed that SCGs have potential prospects as bio-waste modifiers in the application of sustainable pavements.Results demonstrated that the modification process was mainly based on physical reinforcement.Compared with that of the neat asphalt,the shearing stress-resistant ability and high-temperature performance of the SCGs modified binders with the appropriate addition presented a bit of improvement;whereas the binders with 1%and 3%SCGs exhibited remarkably enhanced low-temperature stability.However,notable weaknesses of practical performance were shown for the binder with excessive content of SCGs,indicating the necessity of proportion selecting before application.
