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肝癌细胞对成熟树突状细胞生物物理学特性的影响
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作者 曾柱 龙金华 张芳 《重庆医科大学学报》 CAS CSCD 北大核心 2010年第4期481-484,共4页
目的:研究肝癌细胞微环境成熟树突状细胞(Mature dendritic cells,mDCs)的生物物理学特性的影响,从交叉学科的角度来探索肿瘤细胞的免疫逃逸机制。方法:用免疫磁珠从人外周血分离CD14+单核细胞,加入rhGM-CSF和rhIL-4将单核细胞诱导分化... 目的:研究肝癌细胞微环境成熟树突状细胞(Mature dendritic cells,mDCs)的生物物理学特性的影响,从交叉学科的角度来探索肿瘤细胞的免疫逃逸机制。方法:用免疫磁珠从人外周血分离CD14+单核细胞,加入rhGM-CSF和rhIL-4将单核细胞诱导分化为imDCs,利用TNF-α将未成熟树突状细胞(Immature dendritic cells,imDCs)诱导为mDCs,mDCs与HCCs在Transwell中共培养48h,分别利用微吸管法、荧光偏振法和Transwell法研究细胞粘弹性、膜流动性和迁移能力。结果:与肝癌细胞(Hepa tocellular carcinoma cells,HCCs)共培养后,mDCs的粘弹性和膜流动性显著下降,细胞的迁移能力受到显著的损伤。结论:HCCs可能能够损伤mDCs的生物物理学特性来影响其迁移能力,这可能是肿瘤逃脱机体免疫监视的方式之一,这对进一步深入理解肿瘤的免疫逃逸机制具有重要意义。 展开更多
关键词 树突状细胞 肝癌细胞 生物物理学特性 迁移能力
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ω-3多不饱和脂肪酸对树突状细胞生物物理学特性及迁移能力的影响 被引量:1
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作者 王欢欢 曾柱 +1 位作者 胡祖权 宋萍萍 《医用生物力学》 CAS CSCD 北大核心 2022年第3期433-440,共8页
目的从生物物理学与免疫学交叉角度,分析二十碳五烯酸(eicosapentaenoic acid,EPA)、二十二碳六烯酸(docosahexaenoic acid,DHA)对鼠源树突状细胞(dendritic cells,DCs)生物物理学特性、细胞骨架及迁移能力的影响,探讨ω-3多不饱和脂肪... 目的从生物物理学与免疫学交叉角度,分析二十碳五烯酸(eicosapentaenoic acid,EPA)、二十二碳六烯酸(docosahexaenoic acid,DHA)对鼠源树突状细胞(dendritic cells,DCs)生物物理学特性、细胞骨架及迁移能力的影响,探讨ω-3多不饱和脂肪酸(ω-3 polyunsaturated fatty acids,ω-3 PUFAs)对DCs免疫功能的影响及其潜在作用机制。方法分离C57BL/6J小鼠骨髓来源单核细胞,经20 ng/mL重组鼠集落刺激因子(recombinant mouse granulocyte macrophage colony stimulating factor,rmGM-CSF)和10 ng/mL重组鼠白介素-4(recombinant mouse interleukin-4,rmIL-4)诱导分化为未成熟树突状细胞(immature dendritic cells,imDCs),第6天加入100 ng/mL脂多糖诱导为成熟树突状细胞(mature dendritic cells,mDCs),进一步对imDCs及mDCs进行形态学观察及CD11c阳性率分析;通过细胞增殖检测试剂盒(cell counting kit-8,CCK-8)及流式细胞仪检测不同浓度EPA和DHA(浓度均为0~60μmol/L)作用下DCs细胞活力及凋亡情况;在确定最佳作用浓度后分别通过荧光偏振法、细胞电泳法及浓度梯度法检测分析DCs的膜流动性、电泳迁移率(electrophoretic mobility,EPM)及渗透脆性变化,并用免疫荧光法检测其细胞骨架纤维状肌动蛋白(filamentous actin,F-actin)表达,最后利用Transwell系统检测DCs迁移能力。结果imDCs及mDCs的CD11c阳性率均在80%左右;不同浓度EPA和DHA均呈剂量依赖性降低DCs细胞活力,但并没有诱导其凋亡。在50μmol/LEPA和DHA作用下,DCs生物物理学特性均发生改变,其中渗透脆性和EPM明显下降,膜流动性明显增大。DCs细胞骨架F-actin含量表达均明显上升,迁移率显著下降。结论ω-3 PUFAs可能会通过改变DCs细胞骨架结构及生物物理学特性,抑制细胞迁移能力,进而影响其免疫功能。 展开更多
关键词 Ω-3多不饱和脂肪酸 树突状细胞 生物物理学特性 细胞迁移 细胞骨架
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黄曲霉毒素B1对肝癌细胞HepG2生物物理学特性及细胞骨架结构的影响 被引量:1
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作者 于欢 喻艳琴 +4 位作者 钱天宝 刘清玉 王赟 曾柱 胡祖权 《安徽医科大学学报》 CAS 北大核心 2023年第1期10-14,共5页
目的研究黄曲霉毒素B1(AFB1)对人肝癌细胞(HCCs)生物物理学特性及细胞骨架结构的影响。方法利用0、0.01、0.1、1、5、10μmol/L AFB1分别处理HepG2细胞24 h和48 h,采用CCK-8试剂盒检测细胞活力。在此基础上,分析10μmol/L AFB1对细胞渗... 目的研究黄曲霉毒素B1(AFB1)对人肝癌细胞(HCCs)生物物理学特性及细胞骨架结构的影响。方法利用0、0.01、0.1、1、5、10μmol/L AFB1分别处理HepG2细胞24 h和48 h,采用CCK-8试剂盒检测细胞活力。在此基础上,分析10μmol/L AFB1对细胞渗透脆性、膜流动性、细胞电泳率和F-actin骨架结构的影响。随后,提取细胞总RNA,通过实时荧光定量PCR检测12种主要细胞骨架结合蛋白的转录水平变化。结果AFB1处理48 h时HepG2细胞的活力呈剂量依赖性增强。10μmol/L AFB1处理能够增强HepG2细胞抗低渗能力及细胞电泳率,细胞骨架F-actin含量明显增加,主要细胞骨架结合蛋白的mRNA表达发生改变。结论AFB1能够影响HepG2细胞的生物物理学特性、细胞骨架结构及其结合蛋白,这可能与其毒性作用直接相关。 展开更多
关键词 黄曲霉毒素 肝癌细胞 生物物理学特性 细胞骨架
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急性缺血性卒中栓子生物物理学特性研究进展
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作者 李婧 王怀明 《中国医学装备》 2023年第7期150-156,共7页
急性缺血性卒中(AIS)是导致全球范围内人类死亡和残疾的主要疾病之一,而快速恢复血流以降低脑组织缺血性损伤,是取得更好临床治疗结果的重要原则。机械取栓技术的临床应用显著提升了AIS患者的治疗效果,也开启了以回收栓子为突破点的优... 急性缺血性卒中(AIS)是导致全球范围内人类死亡和残疾的主要疾病之一,而快速恢复血流以降低脑组织缺血性损伤,是取得更好临床治疗结果的重要原则。机械取栓技术的临床应用显著提升了AIS患者的治疗效果,也开启了以回收栓子为突破点的优化治疗策略和设计改进取栓装置的研究途径。回收栓子的组成、结构、硬度和摩擦力等性质与病因诊断、治疗结果及患者预后密切相关。综述研究者利用生物力学、显微结构分析、神经影像学、光谱分析以及“组学”技术等方法分析回收栓子生物物理学特性的成果,旨在深入认识回收栓子的构成和性质,指导卒中患者的精准化及个体化治疗。 展开更多
关键词 急性缺血性卒中(AIS) 栓子 生物物理学特性
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热色液晶热像法客观探究经络的在表征象 被引量:2
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作者 刘骥 张棣 《中国医学创新》 CAS 2012年第26期143-145,共3页
目前,经络的本质以及现象仍然无一个公认的实验证实。本文旨在中医经典理论指导下,利用经络的生物物理特性、经络的热辐射特性与新型技术-热色液晶技术,拟在体表对经络的循经感传现象进行直观、客观的显示。
关键词 经络现象 生物物理学特性 液晶热像法 应用价值
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毫针针刺足三里对失神经肌萎缩大鼠穴区电阻、温度和能量代谢变化的影响
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作者 赖清 王鼎 +4 位作者 吴永强 陈波 张婷 司原成 莫曦雅 《云南中医中药杂志》 2023年第3期82-86,共5页
目的研究大鼠胫前肌失神经萎缩对毫针针刺腧穴穴区电阻、温度及能量代谢的影响,为不同机能状态影响毫针针刺作用差异的生物医学机制提供实验依据。方法将32只清洁级SD大鼠随机分为正常组和肌萎缩组,每组大鼠再配对分入针刺组和非针刺组... 目的研究大鼠胫前肌失神经萎缩对毫针针刺腧穴穴区电阻、温度及能量代谢的影响,为不同机能状态影响毫针针刺作用差异的生物医学机制提供实验依据。方法将32只清洁级SD大鼠随机分为正常组和肌萎缩组,每组大鼠再配对分入针刺组和非针刺组2个亚组。使用A型肉毒素肌肉注射建立大鼠胫前肌化学性失神经支配肌萎缩模型,针刺组针刺“足三里”穴,捻转频率60次/min,行针5 min,留针10 min,共操作3轮后出针,每日操作1次,连续3 d。针刺结束后,测量穴区电阻和温度,切取以穴点为圆心,直径0.5 cm,深度0.5 cm区域的组织块,采用酶联免疫法检测组织中ATP、ADP的含量并计算ATP/ADP。结果针刺正常组大鼠可以降低腧穴的电阻、温度,提高穴区ATP/ADP,差异均有统计学意义(P<0.01);针刺肌萎缩组大鼠能降低穴区电阻、温度,差异均具有统计学意义(P<0.01),且电阻降低幅度显著大于正常组(P<0.01);针刺肌萎缩组大鼠不能提高穴区ATP/ADP,与正常针刺组比较,差异具有显著统计学意义(P<0.01)。结论肌萎缩状态下毫针针刺激活腧穴的作用与正常机体状态存在不同,肌萎缩状态可明显减弱针刺对腧穴ATP/ADP的调节幅度,针刺对肌萎缩的治疗作用可能主要通过增强腧穴电敏特性达成。 展开更多
关键词 毫针针刺 腧穴区域 失神经肌萎缩 生物物理学特性 能量代谢特性
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Wastewater Treatment in the Oasis of Figuig (Morocco) by Facultative Lagoon System: Physico-Chemical and Biological Aspect
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作者 Ouafae El Hachemi Hassan Elhalouani +1 位作者 Antonina Torrens Armengol Miquel Salgot 《Journal of Life Sciences》 2012年第5期543-549,共7页
The community of Figuig is located in a desert area, east of Morocco. It is characterized by an arid climate. Part of the aquifers of the area has a high salinity which creates even worst conditions for the use of the... The community of Figuig is located in a desert area, east of Morocco. It is characterized by an arid climate. Part of the aquifers of the area has a high salinity which creates even worst conditions for the use of the scarce water available in the area. Therefore, it is essential to develop new water resources, especially for agriculture. For this reason, reclaiming wastewater for agricultural use is an essential strategy to increase water resources. The studied system is a wastewater treatment plant (WWTP) with stabilisation ponds; it treats a part of the wastewater of Figuig. The monitoring of the WWTP will allow to the physico-chemical and biological characterization of the treated water, and then, to verify two principal points: (1) the physico-chemical and pathogen parasites elimination; (2) the possibility of the treated water reuse in agricultural irrigation. The reductions recorded at the WWTP show a relatively preferment operation with an average decrease of 3.17 Ulog for faecal coliforms and 50-60% for organic matter. It was observed that the taxonomic density is slow in the WWTP; observed species are generally Cyanobacteria, Euglénophycea and Chlorophycea characteristic of eutrophic water sources. 展开更多
关键词 Wastewater treatment RECLAMATION reuse phytoplankton.
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Phosphorus Use Efficiency of Bio-Based Fertilizers:Bioavailability and Fractionation 被引量:3
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作者 Céline VANEECKHAUTE Joery JANDA +2 位作者 Peter A.VANROLLEGHEM Filip M.G.TACK Erik MEERS 《Pedosphere》 SCIE CAS CSCD 2016年第3期310-325,共16页
Although to date some technologies producing bio-based phosphorus(P) fertilizers have been proposed and implemented, the efficient use of the recovered products is still limited due to legislative constraints and lack... Although to date some technologies producing bio-based phosphorus(P) fertilizers have been proposed and implemented, the efficient use of the recovered products is still limited due to legislative constraints and lack of insights in the P release with time and in the corresponding mechanisms. The aim of this work was to evaluate the fertilizer performance in terms of P release and use efficiency of recovered struvite, FePO_4-sludge, digestate, and animal manure as compared to fossil reserve-based mineral triple superphosphate(TSP). First, product physicochemical characteristics and P fractions in the context of European fertilizer legislation were assessed. Next, a controlled greenhouse experiment was set up to evaluate plant reactions as well as changes of P availability in a sandy soil with high P status and a Rheinsand soil with low P status. Soil P fractions were determined in the extracts with water, ammonium lactate and CaCl_2, and in soil solution sampled with Rhizon soil moisture samplers. Based on all results, it is worth conducting long-term field trials to evaluate the P release effect of struvite and digestate as compared to animal manure and TSP on different soil types with varying P status. These products showed promise as sustainable substitutes for conventional P fertilizers and could contribute to a more efficient use of P in agriculture. A refined classification of P application standards/recommendations in terms of soil P status, soil texture, and fertilizer characteristics, next to the crop P demand, is recommended. Moreover, the additional use of Rhizon samplers for determination of direct available P, including dissolved organic P, is proposed for better understanding and categorization of different P fertilizers in environmental and fertilizer legislations. 展开更多
关键词 chemical soil analysis digestate nutrient recovery Rhizon samplers struvite
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Rheology and microstructure effects of waste spent coffee grounds in modifying asphalt binder
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作者 Mingjun Xie Linglin Xu +3 位作者 Kai Wu Yutong Wen Hongmi Jiang Zhengwu Jiang 《Low-carbon Materials and Green Construction》 2023年第1期21-33,共13页
Herein the biowaste by-product spent coffee grounds(SCGs)from coffee industry were incorporated into asphalt binders for performance enhancement.From the analysis of Fourier transform infrared spectroscopy(FTIR),diffe... Herein the biowaste by-product spent coffee grounds(SCGs)from coffee industry were incorporated into asphalt binders for performance enhancement.From the analysis of Fourier transform infrared spectroscopy(FTIR),differential scanning calorimetry(DSC),dynamic shear rheometer(DSR),and Brookfield viscosity rheometer,it is confirmed that SCGs have potential prospects as bio-waste modifiers in the application of sustainable pavements.Results demonstrated that the modification process was mainly based on physical reinforcement.Compared with that of the neat asphalt,the shearing stress-resistant ability and high-temperature performance of the SCGs modified binders with the appropriate addition presented a bit of improvement;whereas the binders with 1%and 3%SCGs exhibited remarkably enhanced low-temperature stability.However,notable weaknesses of practical performance were shown for the binder with excessive content of SCGs,indicating the necessity of proportion selecting before application. 展开更多
关键词 Spent coffee grounds Asphalt binder Bio-waste management Rheology properties MICROSTRUCTURE
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NONCONSTANT PREY HARVESTING IN RATIO-DEPENDENT PREDATOR-PREY SYSTEM INCORPORATING A CONSTANT PREY REFUGE 被引量:1
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作者 SAPNA DEVI 《International Journal of Biomathematics》 2012年第2期85-104,共20页
This paper deals with the problem of nonconstant harvesting of prey in a ratio-dependent predator-prey system incorporating a constant prey refuge. Here we use the reasonable catch-rate function instead of usual catch... This paper deals with the problem of nonconstant harvesting of prey in a ratio-dependent predator-prey system incorporating a constant prey refuge. Here we use the reasonable catch-rate function instead of usual catch-per-unit-effort hypothesis. The existence, as well as the stability of possible equilibria, is carried out. Bionomic equilibrium of the system is determined and optimal harvest policy is studied with the help of Pontryagin's maximum principle. The key results developed in this paper are illustrated using numer- ical simulations. Our results indicate that dynamic behavior of the system very much depends on the prey refuge parameter and increasing amount of refuge could increase prey density and may lead to the extinction of predator population density. 展开更多
关键词 PREDATOR-PREY prey refuge TAXATION RATIO-DEPENDENT stability bionomic equilibrium HARVESTING optimal equilibrium.
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