AIM: To choose at least one kind of better derivate from 81 novel compounds for further structure-function relationship researches by contrast study on antibacterial activity of three kinds of derivates developed from...AIM: To choose at least one kind of better derivate from 81 novel compounds for further structure-function relationship researches by contrast study on antibacterial activity of three kinds of derivates developed from decanoyl acetaldehyde, a certain kind of ethnodrug and pure synthetics. METHODS: Three quality control strains, staphylococcus aureus ATCC25923, bacillus coli ATCC25922 and bacillus aeruginosus ATCC27853 were used in the preliminary screening in vitro. Some active derivates were selected to carry out the minimal bactericidal concentration test, killing curve test, and the effect of cultivation parameters on minimal inhibitory concentration on clinical separated staphylococcus aureus and MRSA. Acute toxicity test of a few promising derivates were tested preliminarily by intragastric administration to mice. RESULTS: There were significant differences of the antibacterial activity for the three different kinds of derivatives compared to the quality control groups. Results shown that 15 percent of the 53 compounds developed from decanoyl acetaldehyde had special activity of inhibiting staphylococcus aureus ATCC25923. Some optimized derivatives had better activity to clinical separated staphylococcus aureus and MRSA compared to the positive control drug decanoyl acetaldehyde. And the derivates showed stronger inhibitive action to the staphylococcus aureus in basylous circumstance than in acid circumstance. Meanwhile, among 20 derivates developed from a certain kind of ethnodrug, there were 90 percents shown fairly strong and broad antibacterial activity to staphylococcus aureus ATCC25923 and bacillus coli ATCC25922 with high toxicity in mice. And 8 samples of pure synthetics showed weak antibacterial activity. CONCLUSION: The derivates developed from natural products showed fairly well antibacterial activity. And the derivates related to decanoyl acetaldehyde had lower toxcity than that of derivates obtained from the ethnodrug. The pure synthetic compounds had weak activity. It is preferable to do the structure-function relationship research of the derivates related to decanoyl acetaldehyde, and the exploratory study for ethnodrug derivates should be continued if experimental condition permitted. This research has optimized the lead compounds and the result is underway of applying national patent. This study does benefit to the innovative antibacterial drug research that has intellectual property rights. The in-depth systematic researches are continuing.展开更多
基于厌氧氨氧化（anaerobic ammonium oxidation,Anammox）开发的相关工艺在污水脱氮处理中具有重要作用;但Anammox微生物是一类目前尚不能分离和纯培养的微生物,分析其功能微生物的类型,对于研究其生物学机理并改良其工艺具有重要意义...基于厌氧氨氧化（anaerobic ammonium oxidation,Anammox）开发的相关工艺在污水脱氮处理中具有重要作用;但Anammox微生物是一类目前尚不能分离和纯培养的微生物,分析其功能微生物的类型,对于研究其生物学机理并改良其工艺具有重要意义。本研究对变性梯度凝胶电泳（denaturing gradient gel electrophoresis,DGGE）在Anammox微生物应用中的分析条件进行了优化,以提高PCR-DGGE的分析效果。结果表明,采用针对16S r DNA V3区的GC-F341/R518引物组合,PCR最佳退火温度为64℃;DGGE电泳中,最佳上样量为5-7μL,最佳胶浓度为8%,最佳电压/时间为80 V/16 h。优化后的PCR-DGGE具有准确性高和灵敏度好的特点。展开更多
文摘AIM: To choose at least one kind of better derivate from 81 novel compounds for further structure-function relationship researches by contrast study on antibacterial activity of three kinds of derivates developed from decanoyl acetaldehyde, a certain kind of ethnodrug and pure synthetics. METHODS: Three quality control strains, staphylococcus aureus ATCC25923, bacillus coli ATCC25922 and bacillus aeruginosus ATCC27853 were used in the preliminary screening in vitro. Some active derivates were selected to carry out the minimal bactericidal concentration test, killing curve test, and the effect of cultivation parameters on minimal inhibitory concentration on clinical separated staphylococcus aureus and MRSA. Acute toxicity test of a few promising derivates were tested preliminarily by intragastric administration to mice. RESULTS: There were significant differences of the antibacterial activity for the three different kinds of derivatives compared to the quality control groups. Results shown that 15 percent of the 53 compounds developed from decanoyl acetaldehyde had special activity of inhibiting staphylococcus aureus ATCC25923. Some optimized derivatives had better activity to clinical separated staphylococcus aureus and MRSA compared to the positive control drug decanoyl acetaldehyde. And the derivates showed stronger inhibitive action to the staphylococcus aureus in basylous circumstance than in acid circumstance. Meanwhile, among 20 derivates developed from a certain kind of ethnodrug, there were 90 percents shown fairly strong and broad antibacterial activity to staphylococcus aureus ATCC25923 and bacillus coli ATCC25922 with high toxicity in mice. And 8 samples of pure synthetics showed weak antibacterial activity. CONCLUSION: The derivates developed from natural products showed fairly well antibacterial activity. And the derivates related to decanoyl acetaldehyde had lower toxcity than that of derivates obtained from the ethnodrug. The pure synthetic compounds had weak activity. It is preferable to do the structure-function relationship research of the derivates related to decanoyl acetaldehyde, and the exploratory study for ethnodrug derivates should be continued if experimental condition permitted. This research has optimized the lead compounds and the result is underway of applying national patent. This study does benefit to the innovative antibacterial drug research that has intellectual property rights. The in-depth systematic researches are continuing.
文摘基于厌氧氨氧化（anaerobic ammonium oxidation,Anammox）开发的相关工艺在污水脱氮处理中具有重要作用;但Anammox微生物是一类目前尚不能分离和纯培养的微生物,分析其功能微生物的类型,对于研究其生物学机理并改良其工艺具有重要意义。本研究对变性梯度凝胶电泳（denaturing gradient gel electrophoresis,DGGE）在Anammox微生物应用中的分析条件进行了优化,以提高PCR-DGGE的分析效果。结果表明,采用针对16S r DNA V3区的GC-F341/R518引物组合,PCR最佳退火温度为64℃;DGGE电泳中,最佳上样量为5-7μL,最佳胶浓度为8%,最佳电压/时间为80 V/16 h。优化后的PCR-DGGE具有准确性高和灵敏度好的特点。
