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生长素测定方法的研究进展 被引量:1
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作者 谢德玉 《吉首大学学报》 1996年第1期94-96,共3页
本文比较地介绍了有关生长素的测定方法及其研究进展。重点阐述了在分子水平建立起来的生物试法和ELISA法。
关键词 生长素 生物试法 ELISA
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16种海藻中植物生长素的提取、分离纯化和含量测定 被引量:12
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作者 韩丽君 范晓 袁兆惠 《海洋与湖沼》 CAS CSCD 北大核心 2005年第2期167-171,共5页
以荧光分光光度检测法和小麦胚芽鞘切段生物试法对 1 6种中国东部沿海生长的海洋藻类中的生长素含量进行了定量分析。结果表明 ,所测定的 1 6种海藻 ,包括红、绿及褐藻中均有生长素 (IAA)存在。海藻不同种类之间的含量变化较大 ,褐藻中... 以荧光分光光度检测法和小麦胚芽鞘切段生物试法对 1 6种中国东部沿海生长的海洋藻类中的生长素含量进行了定量分析。结果表明 ,所测定的 1 6种海藻 ,包括红、绿及褐藻中均有生长素 (IAA)存在。海藻不同种类之间的含量变化较大 ,褐藻中的含量低于红藻和绿藻。小麦胚芽鞘切段生物试法对IAA测定的含量变化为 5 .3— 1 1 0 .2ng/gFW之间 ,荧光分光光度检测法测定的含量变化为 1 .1— 46.9ng/gFW。小麦胚芽鞘切段生物试法对IAA测定的含量明显高于荧光分光光度检测法测定的相同种含量。讨论结果认为 ,小麦胚芽鞘切段生物试法的测定结果虽然灵敏度高 ,但易受实验材料不均一性的制约 ,试验周期长 ,并受环境因子的干扰 ,适用于海藻中生长素的半定量分析 ;荧光分光光度检测法相对适合于海藻中生长素的定量分析 ,有很好的可行性。 展开更多
关键词 海藻 植物生长素 荧光分光光度检测 小麦胚芽鞘切段生物试法 含量分布
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Purification of Crude Glycerol from Waste Cooking Oil Based Biodiesel Production by Orthogonal Test Method 被引量:4
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作者 Cai Tianfeng Li Huipeng +1 位作者 Zhao Hua Liao Kejian 《China Petroleum Processing & Petrochemical Technology》 SCIE CAS 2013年第1期48-53,共6页
Purification of original crude glycerol obtained from biodiesel production was conducted in a laboratory scale equipment by means of a combined chemical and physical treatment method based upon repeated cycles of acid... Purification of original crude glycerol obtained from biodiesel production was conducted in a laboratory scale equipment by means of a combined chemical and physical treatment method based upon repeated cycles of acidification of liquid phase to the desired pH value by using 5.85% H3PO4 solution for pH value adjustment, and the mixture was kept at 70 ℃ for 60 rain to make phase separation for obtaining a glycerol-rich middle phase. The yield of crude glycerol reached 81.2%. Subsequently, upon reaction of the obtained glycerol phase with 0.03% of sodium oxalate at 80 ℃ for 30 min the impurity removal rate was equal to 19.8%. The fraction boiling between 164 ℃ and 200 ℃ was collected by vacuum distil- lation followed by decolorization with 2% of active carbon at 80 ℃ for two times to yield the product glycerol with an ac- ceptable purity of 98.10%. 展开更多
关键词 crude glycerol sodium oxalate BIODIESEL PURIFICATION glycerol-rich layer
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Role of conventional therapies in the era of biological treatment in Crohn’s disease
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作者 Paolo Gionchetti Carlo Calabrese +7 位作者 Rosy Tambasco Ramona Brugnera Giulia Straforini Giuseppina Liguori Giulia Spuri Fornarini Donatella Riso Massimo Campieri Fernando Rizzello 《World Journal of Gastroenterology》 SCIE CAS CSCD 2011年第14期1797-1806,共10页
Outstanding progress regarding the pathophysiology of Crohn's disease (CD) has led to the development of innovative therapeutic concepts. Numerous controlled trials have been performed in CD. This review concentra... Outstanding progress regarding the pathophysiology of Crohn's disease (CD) has led to the development of innovative therapeutic concepts. Numerous controlled trials have been performed in CD. This review concentrates on the results of randomized,placebo-controlled trials,and meta-analyses when available,that provide the highest degree of evidence. Current guidelines on the management of CD recommend a step-up approach to treatment involving the addition of more powerful therapies as the severity of disease and refractoriness to therapy increase. The advent of biological drugs has opened new therapeutic horizons for treating CD,modifying the treatment goals. However,the large majority of patients with CD will be managed through conventional therapy,even if they are a prelude to biological therapy. 展开更多
关键词 Crohn’s disease SULFASALAZINE 5-Aminosalicylic acid AZATHIOPRINE 6-MERCAPTOPURINE Biological therapies Anti-tumor necrosis facto-α
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In Vitro Activity of Lawsonia inermis (Henna) on Some Pathogenic Fungi
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作者 Elham Abdelbasit Suleiman Elbasheir Ahmmed Mohamed 《Journal of Agricultural Science and Technology(A)》 2014年第8期657-662,共6页
The present study was conducted to investigate antifungal activity ofLawsonia inermis (Henna plant). Leaf samples of the plants were collected from Eastern Nile of Khartoum state, Sudan. Ethanol and petroleum ether ... The present study was conducted to investigate antifungal activity ofLawsonia inermis (Henna plant). Leaf samples of the plants were collected from Eastern Nile of Khartoum state, Sudan. Ethanol and petroleum ether extracts in various concentrations were obtained by maceration (cold method). The extracts were bioassay in vitro to know their bioactivity to inhibit the growth of tested fungi. The cup plate agar diffusion method was adopted to assess the antifungal activity of the extracts against tested yeasts while agar incorporated method was used for other molds. Both extracts revealed anti fungal activity against all yeast strains except Pichiafabianii which was found resistant to both ethanol and ether extracts. The results displayed antifungal activity against tested fungi. Minimum mould concentration (MMC) of the extracts was determined. The obtained results revealed antifungal activity of henna leaves extracts which support the traditional use of the Henna in therapy of fungal infections. The possibility of therapeutic use of Sudanese henna as antifungal agents is recommended. 展开更多
关键词 Lawsonia inermis ANTIFUNGAL ETHER ethanol extracts inhibition zone.
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