[Objective] The paper aims to provide references to cultivate I.galbana in a large scale and explore its medical value.[Method] I.galbana had been cultivated in different light-path flat plate bioreactors,and the grow...[Objective] The paper aims to provide references to cultivate I.galbana in a large scale and explore its medical value.[Method] I.galbana had been cultivated in different light-path flat plate bioreactors,and the growth condition and content of organic matter within cells of I.galbana had been analyzed.[Result] Growth rate and volumetric productivity of I.galbana cells increased as light-path of flat plate bioreactors decreased,however,daily areal output rate rose with the increasing of light path.The smaller the light path of flat plate bioreactors was,the more content of total lipids,protein and polysaccharide it had in I.galbana cells.[Conclusion] It is an effective way to improve production efficiently and reduce the cost by selecting the suitable bioreactors.展开更多
Calopogonium mucunoides (calopo) is one of forage legumes as protein and mineral sources for ruminant livestock in the tropics. However, most of the land in Indonesia that used for forage crops production characteri...Calopogonium mucunoides (calopo) is one of forage legumes as protein and mineral sources for ruminant livestock in the tropics. However, most of the land in Indonesia that used for forage crops production characterized by acid pH, low in nitrogen and phosphorus. Phosphorus fertilizers have been used widely to improve soil fertility in Indonesia. As the high cost of superphosphate (SP), the focus is now on rock phosphate (RP) fertilizer which relatively cheaper than SP, and it has high solubility in acid condition. Combination between nitrogen and phosphorus fertilizer from difference sources may be a promising technique to overcome those problems. A field experiment was conducted on a latosolic soil (low pH, nitrogen and low available Bray II extractable P). The objective of the research is to evaluate the effect of combination between nitrogen and phosphorus fertilizer from difference sources on dry matter production and digestibility, nitrogen and phosphorus uptake of Calopogonium mucunoides (calopo). Completely randomized block design with 9 treatments in 3 replicates was used in this experiment. The treatments were TO (control), TI (SP), T2 (RP), T3 (urea), T4 (ZA), T5 (SP + urea), T6 (SP + ZA), T7 (RP + urea), T8 (RP + ZA). Size of plot was 2 m × 3 m, fertilized with 50 kg N/ha and 200 kg P2O5/ha, according to the assigned treatment. All plot received basal fertilizer of KCI (100 kg KzO/ha) and goat manure (5 ton/ha). Calopogonium mucunoides was defoliated and nutritive value. Result showed that dry matter production on 90 days after planting and analyzed for dry matter production and nutritive value significantly influenzed by the treatments. Combination between N (ZA, urea) and P (SP, RP) fertilizer resulted dry matter production, nutrient uptake, and dry matter digestibility significantly higher compared to control, and NP fertilization separatedly. Combination of RP and N (urea, ZA) resulted in dry matter production, nutrient uptake, and dry matter digestibility non-significantly different compared to SP in combination with N (urea, ZA). Therefore, RP could replace SP if combined with urea or ZA to increase production and nutritive value of Calopogonium mucunoides in acid latosolic soil.展开更多
A differential fatherhood premium exists among selected groups of fathers of various socio-economic backgrounds. Besides marital status, residential arrangement, biological paternity, and other demographic dimensions,...A differential fatherhood premium exists among selected groups of fathers of various socio-economic backgrounds. Besides marital status, residential arrangement, biological paternity, and other demographic dimensions, the mechanisms associated with fathers' structural characteristics of work that produce divergent labor market outcomes are less well understood. The authors leveraged the cases of productivity and specialization, family and responsibility, and compensating differentials and examined the impacts of work context and occupational characteristics on the pay gap between fathers and non-fathers Based on joint data from the 1997-2015 National Longitudinal Survey of Youth (NLSY97) and Occupational Information Network (O'NET) (n -- 37,138), the authors used fixed-effects models to estimate the associations between job features and the fatherhood premium. The regression results showed that occupations entailing competition and requiring on-the-job training are associated with more wage bonus; occupations offering inflexible schedule are associated with less wage gain These work-related characteristics further emphasize fathers' work structure and context, human capital accumulation, and work-family conflict. Overall, these findings are consistent with the theoretical perspectives of productivity and specialization and compensating differentials, adding new evidences to the structural explanations of the fatherhood premium.展开更多
Microalgae lipids/oils are a promising feedstock for biodiesel production. The desired lipids are triacylglycerols. These can either be transesterified to biodiesel or decarboxylated to "green diesel". Increasing mi...Microalgae lipids/oils are a promising feedstock for biodiesel production. The desired lipids are triacylglycerols. These can either be transesterified to biodiesel or decarboxylated to "green diesel". Increasing microalgae lipids production by thermal stressing is important in improving the economics of biodiesel production, but its effectiveness needs to be determined. This paper focuses on the effect of cooling stressing lipid triggering on the microalgae production and lipids yield. Two microalgae species were studied, Chlorella sp. and Dunaliella. In each case, microalgae were grown in two identical 2 L PBRs (photobiorectors) at room temperature. At the end of the exponential growth phase, one PBR was placed in a cold environment while the other PBR was left at room temperature. Microalgae was harvested, freeze dried and the algae oil was extracted. Measurements show that cooling stressing slightly increased the biomass of algae (11% for C2 and 13% for Dunaliella), but it decreased the lipids content of the microalgae, 62% for Chlorella sp. and 13% for Dunaliella. The net effect is a decrease in the lipid production rate (mg lipid/L-day) 58% in case of Chlorella sp., and 2% in case of Dunaliella.展开更多
A highly sensitive and selective 3D excitation-emission fluorescence method has been proposed to rapidly quantify the combined antidiabetics Repaglinide(Re) and Irbesartan(Ir) in rat and human plasmas with the aid of ...A highly sensitive and selective 3D excitation-emission fluorescence method has been proposed to rapidly quantify the combined antidiabetics Repaglinide(Re) and Irbesartan(Ir) in rat and human plasmas with the aid of second-order calibration method based on alternating trilinear decomposition(ATLD) method. Re and Ir with weak fluorescence can be endowed with strong fluorescent property by changing the microenvironment in samples and improving the fluorescence quantum yield by using an appropriate micellar enhanced surfactant. The enhanced excitation-emission matrix fluorescence of Re and Ir can be accurately resolved and can simultaneously attain the optimal concentration even in the presence of a potentially strong intrinsic fluorescence from complex biological matrices, such as rat and human plasmas, by using the ATLD method, which completely exploits the "second-order advantage". The average recoveries of Re and Ir obtained from ATLD with the factor number of 3(N=3) were 101.0%±4.3% and 99.1%±4.1% for rat plasma and 100.5%±5.4% and 97.1%±3.6% for human plasma. Several statistical methods, including Student's t-test, figures of merit, and elliptical joint confidence region, have been utilized to evaluate the accuracy of the proposed method. Results show that the developed method can maintain second-order advantage in simultaneous determinations of the weak fluorescent analytes of interest in different biological plasma matrices.展开更多
基金Supported by National Ministry of Science and Technology Marine‘863’Marine Life Technology Plan Project(2008AA09Z403)Shangdong High School Technological Plan Project(J05I03)~~
文摘[Objective] The paper aims to provide references to cultivate I.galbana in a large scale and explore its medical value.[Method] I.galbana had been cultivated in different light-path flat plate bioreactors,and the growth condition and content of organic matter within cells of I.galbana had been analyzed.[Result] Growth rate and volumetric productivity of I.galbana cells increased as light-path of flat plate bioreactors decreased,however,daily areal output rate rose with the increasing of light path.The smaller the light path of flat plate bioreactors was,the more content of total lipids,protein and polysaccharide it had in I.galbana cells.[Conclusion] It is an effective way to improve production efficiently and reduce the cost by selecting the suitable bioreactors.
文摘Calopogonium mucunoides (calopo) is one of forage legumes as protein and mineral sources for ruminant livestock in the tropics. However, most of the land in Indonesia that used for forage crops production characterized by acid pH, low in nitrogen and phosphorus. Phosphorus fertilizers have been used widely to improve soil fertility in Indonesia. As the high cost of superphosphate (SP), the focus is now on rock phosphate (RP) fertilizer which relatively cheaper than SP, and it has high solubility in acid condition. Combination between nitrogen and phosphorus fertilizer from difference sources may be a promising technique to overcome those problems. A field experiment was conducted on a latosolic soil (low pH, nitrogen and low available Bray II extractable P). The objective of the research is to evaluate the effect of combination between nitrogen and phosphorus fertilizer from difference sources on dry matter production and digestibility, nitrogen and phosphorus uptake of Calopogonium mucunoides (calopo). Completely randomized block design with 9 treatments in 3 replicates was used in this experiment. The treatments were TO (control), TI (SP), T2 (RP), T3 (urea), T4 (ZA), T5 (SP + urea), T6 (SP + ZA), T7 (RP + urea), T8 (RP + ZA). Size of plot was 2 m × 3 m, fertilized with 50 kg N/ha and 200 kg P2O5/ha, according to the assigned treatment. All plot received basal fertilizer of KCI (100 kg KzO/ha) and goat manure (5 ton/ha). Calopogonium mucunoides was defoliated and nutritive value. Result showed that dry matter production on 90 days after planting and analyzed for dry matter production and nutritive value significantly influenzed by the treatments. Combination between N (ZA, urea) and P (SP, RP) fertilizer resulted dry matter production, nutrient uptake, and dry matter digestibility significantly higher compared to control, and NP fertilization separatedly. Combination of RP and N (urea, ZA) resulted in dry matter production, nutrient uptake, and dry matter digestibility non-significantly different compared to SP in combination with N (urea, ZA). Therefore, RP could replace SP if combined with urea or ZA to increase production and nutritive value of Calopogonium mucunoides in acid latosolic soil.
文摘A differential fatherhood premium exists among selected groups of fathers of various socio-economic backgrounds. Besides marital status, residential arrangement, biological paternity, and other demographic dimensions, the mechanisms associated with fathers' structural characteristics of work that produce divergent labor market outcomes are less well understood. The authors leveraged the cases of productivity and specialization, family and responsibility, and compensating differentials and examined the impacts of work context and occupational characteristics on the pay gap between fathers and non-fathers Based on joint data from the 1997-2015 National Longitudinal Survey of Youth (NLSY97) and Occupational Information Network (O'NET) (n -- 37,138), the authors used fixed-effects models to estimate the associations between job features and the fatherhood premium. The regression results showed that occupations entailing competition and requiring on-the-job training are associated with more wage bonus; occupations offering inflexible schedule are associated with less wage gain These work-related characteristics further emphasize fathers' work structure and context, human capital accumulation, and work-family conflict. Overall, these findings are consistent with the theoretical perspectives of productivity and specialization and compensating differentials, adding new evidences to the structural explanations of the fatherhood premium.
文摘Microalgae lipids/oils are a promising feedstock for biodiesel production. The desired lipids are triacylglycerols. These can either be transesterified to biodiesel or decarboxylated to "green diesel". Increasing microalgae lipids production by thermal stressing is important in improving the economics of biodiesel production, but its effectiveness needs to be determined. This paper focuses on the effect of cooling stressing lipid triggering on the microalgae production and lipids yield. Two microalgae species were studied, Chlorella sp. and Dunaliella. In each case, microalgae were grown in two identical 2 L PBRs (photobiorectors) at room temperature. At the end of the exponential growth phase, one PBR was placed in a cold environment while the other PBR was left at room temperature. Microalgae was harvested, freeze dried and the algae oil was extracted. Measurements show that cooling stressing slightly increased the biomass of algae (11% for C2 and 13% for Dunaliella), but it decreased the lipids content of the microalgae, 62% for Chlorella sp. and 13% for Dunaliella. The net effect is a decrease in the lipid production rate (mg lipid/L-day) 58% in case of Chlorella sp., and 2% in case of Dunaliella.
基金supported by the National Natural Science Foundation of China (21205145, 21575039)the Open Funds of State Key Laboratory of Chemo/Biosensing and Chemometrics of Hunan University (201111)The Open Research Program (2015ZD001, 2015ZD002) from the Modernization Engineering Technology Research Center of Ethnic Minority Medicine of Hubei province
文摘A highly sensitive and selective 3D excitation-emission fluorescence method has been proposed to rapidly quantify the combined antidiabetics Repaglinide(Re) and Irbesartan(Ir) in rat and human plasmas with the aid of second-order calibration method based on alternating trilinear decomposition(ATLD) method. Re and Ir with weak fluorescence can be endowed with strong fluorescent property by changing the microenvironment in samples and improving the fluorescence quantum yield by using an appropriate micellar enhanced surfactant. The enhanced excitation-emission matrix fluorescence of Re and Ir can be accurately resolved and can simultaneously attain the optimal concentration even in the presence of a potentially strong intrinsic fluorescence from complex biological matrices, such as rat and human plasmas, by using the ATLD method, which completely exploits the "second-order advantage". The average recoveries of Re and Ir obtained from ATLD with the factor number of 3(N=3) were 101.0%±4.3% and 99.1%±4.1% for rat plasma and 100.5%±5.4% and 97.1%±3.6% for human plasma. Several statistical methods, including Student's t-test, figures of merit, and elliptical joint confidence region, have been utilized to evaluate the accuracy of the proposed method. Results show that the developed method can maintain second-order advantage in simultaneous determinations of the weak fluorescent analytes of interest in different biological plasma matrices.