A reversed-phase high-performance liquid chromatography (RP-HPLC) method was established for the simultaneous determination of three major constituents,/-calycanthine, quercetin, and kaempferol, in Chimonanthi Niten...A reversed-phase high-performance liquid chromatography (RP-HPLC) method was established for the simultaneous determination of three major constituents,/-calycanthine, quercetin, and kaempferol, in Chimonanthi Nitentis Folium. The RP-HPLC analysis was carried out using an Agilent HC-C18 (4.6 mm×250 mm, 5 μm) column and a gradient mobile phase consisting of methanol and phosphate buffer (10:90→90:10, v/v) at a flow rate of 1.0 mL/min. The column temperature was 25 ℃ and the detection wavelength was set at 239 nm from 0 to 15 min and 365 nm from 16 to 45 min. All calibration curves showed good linearity (R2〉0.9991) within test ranges. Relative standard deviations of repeated analyses were less than 2.64% (n = 6), and the recovery of this method was 97.47%-98.26%. The contents of these three analytes were determined for the samples from different harvest times. The results showed that the/-calycanthine content in herbs increases from 99.94 μg/g to 468.0μg/g from April to July, whereas the content of quercetin and kaempferol was higher in April and May than that in June and July, which were consistent with the Chinese traditional medicine's use of Chimonanthi Nitentis Folium in Spring. Herein, a simple, rapid and accurate analytical method is presented and successfully applied for the simultaneous quantitative analysis of alkaloids and flavones from Chimonanthus nitens Oliv.展开更多
Chuanxiong Rhizoma is a widely used Chinese herbal medicine in the past 2000 years.Chuanxiong Rhizoma is composed of volatile oils,phthalide lactones,phenolic acids,polysaccharides and other compounds.To date,more tha...Chuanxiong Rhizoma is a widely used Chinese herbal medicine in the past 2000 years.Chuanxiong Rhizoma is composed of volatile oils,phthalide lactones,phenolic acids,polysaccharides and other compounds.To date,more than 149 compounds in Chuanxiong Rhizoma have been isolated and identified,and some of them have been reported to possess promising biological properties on cardiovascular and central nervous system disorders besides their anti-cancer and antioxidant effects.Modulation of inflammatory mediators and apoptotic factors are believed to contribute to its bioactivities.Analytical methods,such as HPLC,GC and UPLC,are employed for qualitative evaluation of Chuanxiong Rhizoma.In this work,harvest period,growing habitat,processing method and storage,which can affect the quality of Chuanxiong Rhizoma,were also discussed.Comprehensive quality control methods should be developed to ensure the safety,quality and efficacy use of Chuanxiong Rhizoma.Herein,we collected and analyzed the literature of Chuanxiong Rhizoma published on CNKI,ScienceDirect,Springer link,Wiley and PubMed in past two decades,and up-to-date information of Chuanxiong Rhizoma was provided in this paper.We suggested ligustilide,butylidenephthalide and total senkyunolides as the chemical markers to evaluate the quality of Chuanxiong Rhizoma.Additionally,the influences of soil conditions and processing methods on Chuanxiong Rhizoma as future research perspectives should also be further assessed.展开更多
基金National Natural Science Foundation of China(Grant No.81360631)
文摘A reversed-phase high-performance liquid chromatography (RP-HPLC) method was established for the simultaneous determination of three major constituents,/-calycanthine, quercetin, and kaempferol, in Chimonanthi Nitentis Folium. The RP-HPLC analysis was carried out using an Agilent HC-C18 (4.6 mm×250 mm, 5 μm) column and a gradient mobile phase consisting of methanol and phosphate buffer (10:90→90:10, v/v) at a flow rate of 1.0 mL/min. The column temperature was 25 ℃ and the detection wavelength was set at 239 nm from 0 to 15 min and 365 nm from 16 to 45 min. All calibration curves showed good linearity (R2〉0.9991) within test ranges. Relative standard deviations of repeated analyses were less than 2.64% (n = 6), and the recovery of this method was 97.47%-98.26%. The contents of these three analytes were determined for the samples from different harvest times. The results showed that the/-calycanthine content in herbs increases from 99.94 μg/g to 468.0μg/g from April to July, whereas the content of quercetin and kaempferol was higher in April and May than that in June and July, which were consistent with the Chinese traditional medicine's use of Chimonanthi Nitentis Folium in Spring. Herein, a simple, rapid and accurate analytical method is presented and successfully applied for the simultaneous quantitative analysis of alkaloids and flavones from Chimonanthus nitens Oliv.
基金The 12thinnovation activity plan-of undergraduates in the Shanghai University of Traditional Chinese Medicine(Grant No.2019SHUTCM060)the Natural Science Foundation of Shanghai(Grant No.19ZR1457700)+1 种基金2020 New Agricultural Science Research and Reform Practice Project of the Ministry of Education2020 Key Undergraduate Education Reform Project of Shanghai Colleges。
文摘Chuanxiong Rhizoma is a widely used Chinese herbal medicine in the past 2000 years.Chuanxiong Rhizoma is composed of volatile oils,phthalide lactones,phenolic acids,polysaccharides and other compounds.To date,more than 149 compounds in Chuanxiong Rhizoma have been isolated and identified,and some of them have been reported to possess promising biological properties on cardiovascular and central nervous system disorders besides their anti-cancer and antioxidant effects.Modulation of inflammatory mediators and apoptotic factors are believed to contribute to its bioactivities.Analytical methods,such as HPLC,GC and UPLC,are employed for qualitative evaluation of Chuanxiong Rhizoma.In this work,harvest period,growing habitat,processing method and storage,which can affect the quality of Chuanxiong Rhizoma,were also discussed.Comprehensive quality control methods should be developed to ensure the safety,quality and efficacy use of Chuanxiong Rhizoma.Herein,we collected and analyzed the literature of Chuanxiong Rhizoma published on CNKI,ScienceDirect,Springer link,Wiley and PubMed in past two decades,and up-to-date information of Chuanxiong Rhizoma was provided in this paper.We suggested ligustilide,butylidenephthalide and total senkyunolides as the chemical markers to evaluate the quality of Chuanxiong Rhizoma.Additionally,the influences of soil conditions and processing methods on Chuanxiong Rhizoma as future research perspectives should also be further assessed.