Microbial fuel cell(MFC)is a kind of promising clean power supply energy equipment,but serious nonlinearities and disturbances exist when the MFC runs,and it is an important topic to guarantee that the output voltage ...Microbial fuel cell(MFC)is a kind of promising clean power supply energy equipment,but serious nonlinearities and disturbances exist when the MFC runs,and it is an important topic to guarantee that the output voltage reaches the setting value quickly and smoothly.Regulating the feeding flow is an effective way to achieve this goal,and especially,the satisfactory results can be achieved by regulating anode feeding flow.In this work,a feedforward fuzzy logic PID algorithm is proposed.The fuzzy logic system is introduced to deal with the non-linear dynamics of MFC,and corresponding PID parameters are calculated according to defuzzification.The magnitude value of the current density is used to simulate the value of the external load.The simulation results indicate that the MFC output voltage can track the setting value quickly and smoothly with the proposed feedforward fuzzy logic PID algorithm.The proposed algorithm is more efficient and robust with respect to anti-disturbance performance and tracking accuracy than other three control methods.展开更多
Aptamers are molecular recognition elements with high specificity that are selected from deoxyribonucleic acid/ribonucleic acid (DNA/RNA) library. Compared with the traditional protein recognition elements,aptamers ha...Aptamers are molecular recognition elements with high specificity that are selected from deoxyribonucleic acid/ribonucleic acid (DNA/RNA) library. Compared with the traditional protein recognition elements,aptamers have excellent properties such as cost-effective,stable,easy for synthesis and modification. In recent years,electrochemistry plays an important role in biosensor field because of its high sensitivity,high stability, fast response and easy miniaturization. Through the combination of these two technologies and our rational design,we constructed a series of biosensors and biochips that are simple,fast,cheap and miniaturized. Firstly,we designed an adenosine triphosphate (ATP) electrochemical biosensor based on the strand displacement strategy. We can detect as low as 10 nmol/L of ATP both in pure solution and complicated cell lysates. Secondly,we creatively split the aptamers into two fragments and constructed the sandwich assay platform only based on single aptamer sequence. We successfully transferred this design on biochips with multiple micro electrodes (6×6) and accomplished multiplex detection. In the fields of biochips and biocomputers,we designed several DNA logic gates with electric (electrochemical) signal as output which paves a new way for the development of DNA computer.展开更多
基金Project(61563032)supported by the National Natural Science Foundation of ChinaProject(18JR3RA133)supported by Gansu Basic Research Innovation Group,China
文摘Microbial fuel cell(MFC)is a kind of promising clean power supply energy equipment,but serious nonlinearities and disturbances exist when the MFC runs,and it is an important topic to guarantee that the output voltage reaches the setting value quickly and smoothly.Regulating the feeding flow is an effective way to achieve this goal,and especially,the satisfactory results can be achieved by regulating anode feeding flow.In this work,a feedforward fuzzy logic PID algorithm is proposed.The fuzzy logic system is introduced to deal with the non-linear dynamics of MFC,and corresponding PID parameters are calculated according to defuzzification.The magnitude value of the current density is used to simulate the value of the external load.The simulation results indicate that the MFC output voltage can track the setting value quickly and smoothly with the proposed feedforward fuzzy logic PID algorithm.The proposed algorithm is more efficient and robust with respect to anti-disturbance performance and tracking accuracy than other three control methods.
基金100 Talents Program of Chinese Academy of SciencesNational Key Basic Research Program of China ("973"Program) (No. 2012CB932600)
文摘Aptamers are molecular recognition elements with high specificity that are selected from deoxyribonucleic acid/ribonucleic acid (DNA/RNA) library. Compared with the traditional protein recognition elements,aptamers have excellent properties such as cost-effective,stable,easy for synthesis and modification. In recent years,electrochemistry plays an important role in biosensor field because of its high sensitivity,high stability, fast response and easy miniaturization. Through the combination of these two technologies and our rational design,we constructed a series of biosensors and biochips that are simple,fast,cheap and miniaturized. Firstly,we designed an adenosine triphosphate (ATP) electrochemical biosensor based on the strand displacement strategy. We can detect as low as 10 nmol/L of ATP both in pure solution and complicated cell lysates. Secondly,we creatively split the aptamers into two fragments and constructed the sandwich assay platform only based on single aptamer sequence. We successfully transferred this design on biochips with multiple micro electrodes (6×6) and accomplished multiplex detection. In the fields of biochips and biocomputers,we designed several DNA logic gates with electric (electrochemical) signal as output which paves a new way for the development of DNA computer.
